http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Rice mutant resources for gene discovery
Hirohiko Hirochika,,Emmanuel Guiderdoni,An, Gyn-heung,Yue-ie Hsing,Eun, Moo-Young,Han, Chang-deok,Narayana Upadhyaya,Hei Leung,Srinivasan Ramachandran,Qifa Zhang,Andy Pereira,Venkatesan Sundaresan Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional analyses, extensive international collaboration is needed for this next goal. The aims of this review are to summarize the current status of rice mutant resources, key tools for functional analysis of genes, and our perspectives on how to accelerate rice gene discovery through collaboration.
LEE, SANG-KYU,JEON, JONG-SEONG,BÖ,RNKE, FREDERIK,VOLL, LARS,CHO, JUNG-IL,GOH, CHANG-HYO,JEONG, SUK-WON,PARK, YOUN-IL,KIM, SUNG JIN,CHOI, SANG-BONG,MIYAO, AKIO,HIROCHIKA, HIROHIKO,AN, GYNHEUNG,CHO, Blackwell Publishing Ltd 2008 Plant, cell and environment Vol.31 No.12
<P>ABSTRACT</P><P>During photosynthesis, triose-phosphates (trioseP) exported from the chloroplast to the cytosol are converted to sucrose via cytosolic fructose-1,6-bisphosphatase (cFBPase). Expression analysis in rice suggests that OscFBP1 plays a major role in the cytosolic conversion of trioseP to sucrose in leaves during the day. The isolated <I>OscFBP1</I> mutants exhibited markedly decreased photosynthetic rates and severe growth retardation with reduced chlorophyll content, which results in plant death. Analysis of primary carbon metabolites revealed both significantly reduced levels of sucrose, glucose, fructose and starch in leaves of these mutants, and a high accumulation of sucrose to starch in leaves of rice plants. In the <I>oscfbp1</I> mutants, products of glycolysis and the TCA cycle were significantly increased. A partitioning experiment of <SUP>14</SUP>C-labelled photoassimilates revealed altered carbon distributions including a slight increase in the insoluble fraction representing transitory starch, a significant decrease in the neutral fraction corresponding to soluble sugars and a high accumulation of phosphorylated intermediates and carboxylic acid fractions in the <I>oscfbp1</I> mutants. These results indicate that the impaired synthesis of sucrose in rice cannot be sufficiently compensated for by the transitory starch-mediated pathways that have been found to facilitate plant growth in the equivalent <I>Arabidopsis</I> mutants.</P>