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Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection
Wang, Yiming,Kwon, Soon Jae,Wu, Jingni,Choi, Jaeyoung,Lee, Yong-Hwan,Agrawal, Ganesh Kumar,Tamogami, Shigeru,Rakwal, Randeep,Park, Sang-Ryeol,Kim, Beom-Gi,Jung, Ki-Hong,Kang, Kyu Young,Kim, Sang Gon,K The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.4
Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.
김정아,조경원,Raksha Singh,Young-Ho Jung,Seung-Hee Jeong,So-Hee Kim,Jae-eun Lee,Yoon-Seong Cho,Ganesh K. Agrawal,Randeep Rakwal,Shigeru Tamogami,Birgit Kersten,전종성,Gynheung An,Nam-Soo Jwa 한국분자세포생물학회 2009 Molecules and cells Vol.28 No.5
Rice Oryza sativa accelerated cell death and resistance 1 (OsACDR1) encodes a putative Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of-function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal patho-gen (Magnaporthe grisea) and showed inhibition of appres-sorial penetration on the leaf surface. In contrast, loss-of-function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1:GFP fusion protein in rice protoplast and onion epidermal cells revealed its local-ization to the nucleus. These results indicate that OsACDR1 plays an important role in the positive regulation of disease resistance in rice.