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Enhancement of potency and stability of human extracellular superoxide dismutase
( Sung Hwan Kim ),( Hae Young Kim ),( Jung Ho Kim ),( Jung Hye Choi ),( Won Kook Ham ),( Yoon Jae Jeon ),( Hara Kang ),( Tae Yoon Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.2
Cells express several antioxidant enzymes to scavenge reactive oxygen species (ROS) responsible for oxidative damages and various human diseases. Therefore, antioxidant enzymes are considered biomedicine candidates. Among them, extracellular superoxide dismutase (SOD3) had showed prominent efficacy against asthma and inflammation. Despite its advantages as a biomedicine, the difficulty in obtaining large quantity of active recombinant human SOD3 (rhSOD3) has limited its clinical applications. We found that a significant fraction of overexpressed rhSOD3 was composed of the inactive apo-enzyme and its potency against inflammation depended on the rate of metal incorporation. Also, purified rhSOD3 was unstable and lost its activity very quickly. Here, we suggest an ideal preparative method to express, purify, and store highly active rhSOD3. The enzymatic activity of rhSOD3 was maximized by incorporating metal ions into rhSOD3 after purification. Also, albumin or polyethylene glycol prevented rapid inactivation or degradation of rhSOD3 during preparative procedures and long-term storage. [BMB Reports 2015; 48(2): 91-96]
Bahk, S. Y.,Chang, S. D.,Cheon, B. G.,Cho, J. H.,Jang, H. I.,Hahn, C. H.,Hara, T.,Lim, G. Y.,Kang, J. S.,Kim, C. O.,Kim, J. Y.,Kim, K. Y.,Kim, S. N.,Kim, T. I.,Kim, T. Y.,Koo, D. G.,Lee, S. B.,Lim, I. 慶尙大學校 기초과학연구소 1991 基礎科學硏究所報 Vol.7 No.-
An angular method of identifying diffractive excitation(DE) events for interactions of a hadron beam in nuclear emulsion is applied to identifying DE events in interactions of heavy ions beams. The "apparent" mean free paths (MEP) of DE processes for ^16O(^28Si) beams are 1.00±0.12, 2.4^_1.6_-0.7, and 2.2±0.4(1.5±0.2)m, respectively, at 200, 60 and 14.6 GeV/nucleon, which corresponds to 20-10% fo the MFP for total inelastic interactions. Distinctive features of diffractively excited nuclei are discussed.
Kim, Dae Hwan,Choi, Sungju,Jang, Juntae,Kang, Hara,Kim, Dong Myong,Choi, Sung-Jin,Kim, Yong-Sung,Oh, Saeroonter,Baeck, Ju Heyuck,Bae, Jong Uk,Park, Kwon-Shik,Yoon, Soo Young,Kang, In Byeong SOCIETY FOR INFORMATION DISPLAY 2017 Journal of the Society for Information Display Vol. No.
<P>Proposed decomposition is validated by reproducing the PBTS time-evolution of I-V characteristics through the technology computer-aided design simulation into which the extracted DOS and charge trapping are incorporated. It is also found that the quantitative decomposition of PBTS-induce Delta V-T accompanied with the multiple stretched-exponential models enables an effective assessment of the complex degradation nature of multiple PBTS physical processes occurring simultaneously. Our results can be easily applied universally to any device with any stress conditions, along with guidelines for process optimization efforts toward ultimate PBTS stability.</P>
Ultralight shape memory actuator from wire-woven Kagome truss
Kim, Hara,Kang, Kiju Elsevier 2017 International journal of mechanical sciences Vol.134 No.-
<P><B>Abstract</B></P> <P>In this paper, a novel actuator in the form of a Kagome truss composed of shape memory alloy (SMA) wires and common metallic wires is described. In the actuator, the SMA wires play dual roles as load supporters and actuating elements. The intersections between the SMA wires and between the SMA wires and the common metallic wires were joined by using shrinkable tubes, which induced the kinematically compatible buckling of the columns of SMA wires under compression, consequently enabling the large actuation displacement of up to 30% of the height with some sacrifice of the load capacity. An analytic solution was derived using the elementary mechanics of buckling and geometric relation of curved column, which gave fairly good estimation of the behavior of the Kagome actuator for the deformation and actuation stages, based on the behavior of a single SMA wire. The lightness and large actuation range of this actuator appear to be useful for practical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An actuator of a Kagome truss composed of shape memory alloy wires is described. </LI> <LI> This actuator produced a large actuation of 30% under compression with reproducibility. </LI> <LI> An analytic solution was derived using the geometry and mechanics of buckling. </LI> <LI> The behavior could be estimated well based on the behavior of a single SMA wire. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
One-Dimensional Molecular Zippers
Kim, Hyo Won,Jung, Jaehoon,Han, Mina,Lim, Seongjoon,Tamada, Kaoru,Hara, Masahiko,Kawai, Maki,Kim, Yousoo,Kuk, Young American Chemical Society 2011 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.133 No.24
<P>We synthesized an azobenzene derivative to demonstrate a one-dimensional molecular zipper. The formation and underlying mechanism of the molecular zipper formed by combined hydrogen-bonding and van der Waals interactions between adjacent molecules were investigated on a Au(111) surface using scanning tunneling microscopy and density functional theory calculations.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2011/jacsat.2011.133.issue-24/ja2031486/production/images/medium/ja-2011-031486_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja2031486'>ACS Electronic Supporting Info</A></P>
Hara Kang,Jun Tae Jang,Jonghwa Kim,Sung-Jin Choi,Dong Myong Kim,Dae Hwan Kim 대한전자공학회 2015 Journal of semiconductor technology and science Vol.15 No.5
Positive bias stress-induced instability in amorphous indium-gallium-zinc-oxide (a-IGZO) bottom-gate thin-film transistors (TFTs) was investigated under high VGS/low VDS and low VGS/high VDS stress conditions through incorporating a forward/reverse VGS sweep and a low/high VDS readout conditions. Our results showed that the electron trapping into the gate insulator dominantly occurs when high VGS/low VDS stress is applied. On the other hand, when low VGS/high VDS stress is applied, it was found that holes are uniformly trapped into the etch stopper and electrons are locally trapped into the gate insulator simultaneously. During a recovery after the high VGS/low VDS stress, the trapped electrons were detrapped from the gate insulator. In the case of recovery after the low VGS/high VDS stress, it was observed that the electrons in the gate insulator diffuse to a direction toward the source electrode and the holes were detrapped to out of the etch stopper. Also, we found that the potential profile in the a-IGZO bottom-gate TFT becomes complicatedly modulated during the positive VGS/VDS stress and the recovery causing various threshold voltages and subthreshold swings under various read-out conditions, and this modulation needs to be fully considered in the design of oxide TFT-based active matrix organic light emitting diode display backplane.
Reproductive Cycle of the Female Grey Mullet, Mugil cephalus, on the Coast of Jeju Island, Korea
Kim, Sung Jun,Lee, Young Don,Yeo, In Kyo,Baek, Hea Ja,Kim, Hyung Bae,Nagae, Masaki,Soyano, Kiyoshi,Hara, Akihiko 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.1
숭어의 생식주기를 밝히기 위해 2002년 2월부터 2003년 3월까지 제주도 동남부 연안에서 채집하였고, 난모세포의 발달과정을 조직학적 방법으로 관찰하였다. 난모세포의 발달과정은 GSI 변화와 밀접하였다. GSI는 9월에 급격히 증가하여 12월에 최고 값을 나타내었다. 난소의 조직학적 관찰결과, 염색인기와 주변인기의 난모세포는 연중 관찰되었으며, 8월에는 일부에서 난황을 포함하는 난모세포가 관찰되었다. 대부분의 난모세포가 10월과 12월에는 난황구가 단계의 난모세포로 발달했다. 10월과 12월에 난황구기 난모세포들을 가진 개체의 출현이 가장 높았으며, 잔존여포와 퇴화 난모세포는 2월부터 관찰되었다. 숭어의 주산란기는 11월에서 1월이고, 난군동기발달형의 난소를 가지며 외양으로 이동하여 산란하는 어류에 속한다.
Kang, Hara,Jang, Jun Tae,Kim, Jonghwa,Choi, Sung-Jin,Kim, Dong Myong,Kim, Dae Hwan The Institute of Electronics and Information Engin 2015 Journal of semiconductor technology and science Vol.15 No.5
Positive bias stress-induced instability in amorphous indium-gallium-zinc-oxide (a-IGZO) bottom-gate thin-film transistors (TFTs) was investigated under high $V_{GS}$/low $V_{DS}$ and low $V_{GS}$/high $V_{DS}$ stress conditions through incorporating a forward/reverse $V_{GS}$ sweep and a low/high $V_{DS}$ read-out conditions. Our results showed that the electron trapping into the gate insulator dominantly occurs when high $V_{GS}$/low $V_{DS}$ stress is applied. On the other hand, when low $V_{GS}$/high $V_{DS}$ stress is applied, it was found that holes are uniformly trapped into the etch stopper and electrons are locally trapped into the gate insulator simultaneously. During a recovery after the high $V_{GS}$/low $V_{DS}$ stress, the trapped electrons were detrapped from the gate insulator. In the case of recovery after the low $V_{GS}$/high $V_{DS}$ stress, it was observed that the electrons in the gate insulator diffuse to a direction toward the source electrode and the holes were detrapped to out of the etch stopper. Also, we found that the potential profile in the a-IGZO bottom-gate TFT becomes complicatedly modulated during the positive $V_{GS}/V_{DS}$ stress and the recovery causing various threshold voltages and subthreshold swings under various read-out conditions, and this modulation needs to be fully considered in the design of oxide TFT-based active matrix organic light emitting diode display backplane.
다문화 가족 어머니들의 유아기 자녀양육 경험에 대한 해석현상학적 연구
강하라 ( Hara Kang ),이운경 ( Woon Kyung Lee ),김수지 ( Soojee Kim ),윤기봉 ( Kibong Yun ),김민정 ( Min-jung Kim ),도현심 ( Hyun-sim Doh ) 한국가족복지학회 2017 한국가족복지학 Vol.22 No.2
This study aimed to explore parenting experiences of preschool-age children`s mothers from multi-cultural families. An interpretive phenomenological approach was applied to better understand how the mothers perceive and describe their parenting experiences as marriage immigrant women. Data were collected through two semi-structured, in-depth interviews for 10 participants and analyzed by the interpretive phenomenological method suggested by Smith and Osborn<sup>[52].</sup> Results showed interconnected relations among the participants` parental beliefs based on mother country`s culture, acculturation to Korean culture, parenting behaviors, and self-awareness/evaluation as a parent. Two overarching themes, `comparisons of parenting behaviors between Korean mothers and multi-cultural mothers` and `parenting experience of multi-cultural family mothers as immigrants in Korea society,` emerged. With understanding of multi-cultural family mother`s parenting as an organic whole, proper and systematic intervention approaches can be developed.