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Transfer of Listeria monocytogenes between Abiotic Surfaces under Different Weights
Hai Yen Lee,Lay Ching Chai,Nor Ainy Mahyudin,Mitsuaki Nishibuchi,Radu Son 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
Cross contamination of Listeria monocytogenesfrom two different surfaces (plastic wrappers and stainlesssteel coupons) was simulated using a series of differentweights. Enumeration of transfer was based on surfacebacterial counts of biofilm stained surfaces. Direct contactbetween 2 surfaces has a constant rate of transfer that isindependent of the pressure applied. This is the first reporton the study of cross contamination between surfaces usingpressure to illustrate transfer of bacteria in a foodprocessing line.
( Hai Yen Lee ),( Lay Ching Chai ),( Chai Fung Pui ),( Woan Chwen Wong ),( Shuhaimi Mustafa ),( Yoke Kqueen Cheah ),( Zuraini Mat Issa ),( Mitsuaki Nishibuchi ),( Ra Du Son ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.9
There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures (60oC and -20oC) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to induce a severe cell injury (up to 100% injury). The injured cells were then subjected to the US Food and Drug Administration (FDA), the ISO-11290, and the modified United States Department of Agriculture (mUSDA) protocols, and plated on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar Listeria for 24 h or 48 h. The evaluation of the total recovery of injured cells was also calculated based on the costs involved in the preparation of media for each protocol. Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze-injured, and heat-freeze-injured cells and was shown to be the most cost effective for heat-freeze-injured cells.
마이해엔,이두형,Mai, Hai Yen,Lee, Du-Hyeong The Korean Academy of Prosthodonitics 2019 대한치과보철학회지 Vol.57 No.3
목적:컴퓨터 가이드 임플란트 수술에서 방사선 영상과 디지털 영상과의 정합은 필수적인 과정이다. 본 연구의 목적은 마이크로스크류의 사용이 가이드 수술을 위한 영상정합 과정에서 작업시간과 술자편의성에 미치는 영향을 조사하는 것이다. 재료 및 방법: 연구모형의 제작을 위해 Kennedy class I 하악 덴티폼에 마이크로스크류 2개를 후구치 삼각 부위에 식립 후 컴퓨터 단층촬영과 디지털 스캔을 통해 방사선 영상과 스캔 영상을 획득하였다. 영상들은 잔존 치아 부위만을 이용하는 방법과 치아와 마이크로스크류를 이용하는 방법을 이용하여 12명의 술자에 의해 중첩되었다. 이 중첩된 영상에 대하여 작업시간, 술자편의성, 만족도의 자료가 수집되고, Mann-Whitney U test을 통해 분석되었다. 결과: 작업시간은 영상정합 조건 사이에 통계학적인 차이가 없었다 (P > .05). 술자편의성과 만족도는 치아와 마이크로스크류를 이용하는 방법에서 잔존 치아 부위만을 이용하는 방법보다 높았다 (P < .001). 결론: 영상정합에서 마이크로스크류의 이용은 작업시간의 단축에는 영향을 미치지 않지만 술자편의성과 만족도는 향상시킨다. Purpose: The image registration of radiographic image and digital surface data is essential in the computer-guided implant guide system. The purpose of this study was to examine the effects of using micro-screw on the working time and convenience of operators in the process of image matching for guided implant surgery. Materials and methods: A mandibular dental model was prepared in partial edentulism for Kennedy class I classification. Two micro-screws were placed on the each side of retromolar area. Radiographic and scan images were taken using computed-tomography and digital scanning. The images were superimposed by 12 operators in software in two different conditions: using remaining teeth image alone and using teeth and micro-screws images. Working time, operator convenience and satisfaction were obtained, and analyzed using the Mann-Whitney U test (${\alpha}=.05$). Results: The working time was not statistically different between image registration conditions (P>.05); however, operator convenience and satisfaction were higher in the teeth and micro-screw assisted condition than in the teeth-alone assisted condition (P<.001). Conclusion: The use of micro-screw for the image registration has no effect in working time reduction, but improves operator convenience and satisfaction.
마이해엔,최용도,이두형,Mai, Hai Yen,Choi, Yong-Do,Lee, Du-Hyeong The Korean Academy of Prosthodonitics 2019 대한치과보철학회지 Vol.57 No.4
본 임상증례는 전치부 영역에서 안면영상, 콘빔 컴퓨터 단층촬영 그리고 구내 스캔영상을 이용하여 보철물을 시각화하는 과정을 보여준다. 이러한 접근 방식은 임상과 기공 그리고 환자 측과의 소통을 향상시킨다. 환자가 상악 중절치의 외상 후 보철치료를 위해 내원하였고, 전신 건강 상태는 양호하였다. 안면연상, 콘빔 컴퓨터 단층촬영 그리고 구내 스캔영상을 이용하여 가상 환자 모형을 제작하였다. 최종 보철물의 제작 전 보철물 디자인 영상이 환자에게 제시되었고, 환자의 의견을 반영하여 형태를 수정하였다. 이러한 디지털 작업 과정은 최적의 심미 보철물의 제작을 용이하게 하고, 보철물의 예측성을 향상시킨다. This clinical case report describes the digital workflow that combines a face scan, cone beam computed tomography and an intraoral scan to visualize the outcome of prosthodontic treatment in the anterior region. This approach improves communication between clinic, laboratory and patients. A patient with healthy general condition came for a restorative treatment to treat post-traumatic central incisors of maxilla. A virtual patient replica was made by incorporating a face scan, cone beam computed tomography and an intraoral scan. Design mockup of definitive restorations was shown to the patient and modified according to the patient's desire. This digital workflow facilitates the fabrication of optimal esthetic restorations, and enhances the predictability of outcome of restorations.
Chang, Chih-Chun,Lee, Yi-Chen,Tsai, Huang-Wen,Yii, Shyi-Chun,Yen, Tzung-Hai,Chu, Fang-Yeh Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13
Purpose: To examine the effectiveness of serum free-to-total prostate specific antigen ratio (%fPSA) for the detection of prostate cancer (PCa) in men with different serum total PSA (tPSA) categories. Materials and Methods: From January 2010 to December 2013, a total of 225 patients with lower urinary tract symptoms (LUTS) underwent tPSA and %fPSA measurements. Histological examination with calculation of Gleason score and whole body bone scans were performed in identified cases of PCa. Results: PCa was diagnosed in 44 (19.6%) patients and the remaining 181 patients had benign prostate disease. PCa was detected in 5 (23.8%), 13 (8.7%) and 26 (47.3%) cases with tPSA level ranges ${\leq}4ng/ml$, 4 to 10 ng/ml and >10 ng/ml, respectively. The average Gleason score was $7.2{\pm}0.2$. Some 6 (13.6%) out of 44 PCa patients had bone metastases. The sensitivity was 80% and specificity was 81.3% at the cut-off %fPSA of 15% in PCa patients with a tPSA level below 4 ng/mL. A lower %fPSA was associated with PCa patients with Gleason score ${\geq}7$ than those with Gleason score ${\leq}6$ ($11.7{\pm}0.98$ vs. $16.5{\pm}2.25%$, P=0.029). No obvious relation of %fPSA to the incidence of bone metastasis was apparent in this study. Conclusions: The clinical application of %fPSA could help to discriminate PCa from benign prostate disease in men with a tPSA concentration below 4 ng/mL.
Fatty acids fouling on forward osmosis membrane: impact of pH
Ruengruehan, K.,Kim, H.,Hai Yen, L.T.,Jang, A.,Lee, W.,Kang, S. Balaban Publishers 2016 Desalination and Water Treatment Vol.57 No.16
<P>The fouling propensity of the fatty acids is investigated during the operation of the osmotically driven membrane process. Experiments were performed at various pH values ranged from 4.0 to 9.0 in the presence of the octanoic acid as the model fatty acids. Results demonstrated that the fatty acid fouling was significantly dependent on the changes of pH. In all tested pH values, permeate flux was sharply decreased during the initial coverage of fatty acids on the membrane surface, then slowly decreased as the fatty acid layer became developed. At pH 9.0, fouling propensity was less than those of pH values around 4.9 (pK(a) of octanoic acid) due to the protonation of the carboxylic group of octanoic acid molecules, which resulted in electrostatic repulsion between octanoic acid molecules and membrane surfaces. The measurement of contact angle and FTIR spectra also supports that more octanoic acid molecules are adsorbed on FO membrane surfaces at the low pH than at the high pH.</P>