High expression of keratin 6C is associated with poor prognosis and accelerates cancer proliferation and migration by modulating epithelial–mesenchymal transition in lung adenocarcinoma

Hai‑Bo Hu,Xiao‑Ping Yang,Pei‑Xia Zhou,Xin‑Ai Yang,Bin Yin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background Lung adenocarcinoma (LUAD) is a more frequent subtype of lung cancer and most cases are discovered in the late stages. The proliferation and metastasis of LUAD are pivotal for disease progression. Despite unremitting deeper understanding of LUAD biology, the mechanisms involved in the proliferation and metastasis of LUAD remain unclear. The objective of our article was to inquiry the expression and the function of keratin 6C (KRT6C) in LUAD cells. Methods First, the expression level and prognostic value of KRT6C in LUAD tissues were analyzed on the basis of the data acquired from TCGA database. Through qRT-PCR, the expression level of KRT6C on LUAD cell lines (A549, H1299, PC-9) and human normal lung cell line MRC-5 was tested. After that, CCK8 and colony formation assays was utilized to detect cell proliferation. In addition, to explore the influence of KRT6C on LUAD migration and invasion ability, scratch wound healing and transwell assays were utilized. Through western blotting, the protein expression levels of KRT6C, PCNA, E-cadherin, N-cadherin, Snail and Vimentin were detected. Results The outcomes revealed that KRT6C was highly expressed in LUAD tissues and cell lines. Besides, elevated level of KRT6C was related to worse prognosis in LUAD patients. Ablation of KRT6C restrained proliferation, migration and invasion of A549 cells. KRT6C deficiency augmented the expression of E-cadherin as well as reduced the expression of N-cadherin, Snail and Vimentin. Conclusion Above all, these consequences indicated that depletion of KRT6C suppressed A549 cell proliferation, migration and invasion, which might be achieved by regulating EMT. In general, KRT6C is identified as a potential therapeutic target for LUAD.

Effect of Ausforming on the Stability of Retained Austenite in a C-Mn-Si Bainitic Steel

Hai-jiang Hu,Guang Xu,Li Wang,Ming-xing Zhou,Zheng-liang Xue 대한금속·재료학회 2015 METALS AND MATERIALS International Vol.21 No.5

The effect of ausforming on the stability of retained austenite in a C-Mn-Si bainitic steel was investigated through metallography, X-ray diffraction and dilatometry. The geometrical relationships of the amount of bainite transformation and the volume fractions of retained austenite with deformation strains were studied. The results show that the degree of promotion of small strains on bainite transformation is nonlinear because of the dual effects of accelerated nucleation and retarded growth caused by ausforming. The transformed bainite fraction first increased and then decreased with increased small strains. It indicates that there is a maximum degree of the promotional function corresponding to a certain small strain at low temperature. Although small strains promote bainite transformation, a larger quantity of retained austenite exists at room temperature due to the suppressed martensite transformation during the cooling process after bainite transformation. The carbon content in retained austenite increases with the amount of baintie transformation, which contributes to the stability of austenite. Compared with the stabilizing effect due to carbon enrichment, mechanical stabilization caused by ausforming has a decisive effect on determining the volume fraction of retained austenite after isothermal bainite transformation.

METRIC THEOREM AND HAUSDORFF DIMENSION ON RECURRENCE RATE OF LAURENT SERIES

Hu, Xue-Hai,Li, Bing,Xu, Jian Korean Mathematical Society 2014 대한수학회보 Vol.51 No.1

We show that the recurrence rates of Laurent series about continued fractions almost surely coincide with their pointwise dimensions of the Haar measure. Moreover, let $E_{{\alpha},{\beta}}$ be the set of points with lower and upper recurrence rates ${\alpha},{\beta}$, ($0{\leq}{\alpha}{\leq}{\beta}{\leq}{\infty}$), we prove that all the sets $E_{{\alpha},{\beta}}$, are of full Hausdorff dimension. Then the recurrence sets $E_{{\alpha},{\beta}}$ have constant multifractal spectra.

The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins

Hu, Jian-hong,Li, Qing-Wang,Li, Gang,Chen, Xiao-Yu,Hai-Yang, Hai-Yang,Zhang, Shu-Shan,Wang, Li-Qiang Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.4

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been made to evaluate the effect of LDL on boar sperm motility and other characteristics. The experiment was carried out to investigate the effect of LDL on the freezing of boar sperm in 0.25 ml straws. The aim was to evaluate the quality of boar spermatozoa cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Tris-citric acid-glucose (TCG) and Tris-citric acid-fructose (TCF), two basic freezing extenders containing egg yolk. Similarly, acrosome and plasma membrane integrity were also evaluated and compared to semen cryopreserved with TCG and TCF. Analysis of sperm quality after freeze-thaw showed that the motility, acrosome and plasma membrane integrity were improved with LDL in the extender, as compared to the TCG and TCF. The highest post-thaw integrity of acrosome and plasma membrane and motility were obtained with 9% LDL (w/v). Consequently, the optimum LDL concentration in the extender was 9%. It is also suggested that the concentration of LDL addition is important for the effect on boar sperm protection during freezing and thawing. The percentage of motile spermatozoa was significantly higher after freezing in 9% LDL than in TCG and TCF 54.4% versus 30.4% and 30.1% (p<0.05), respectively. The integrity of acrosome and plasma membrane were also significantly higher at 70.3% and 50.5% respectively with semen frozen in 9% LDL extender compared to TCG at 37.8% and 30.3% and TCF at 36.4% and 29.9%, respectively (p<0.05),. In conclusion, we propose that extender containing LDL extracted from hen egg yolk could be used as a cryoprotective media with a better efficiency than TCG and TCF. LDL improved boar semen quality, allowing better spermatozoa motility, acrosome and plasma membrane integrity after the freeze-thaw process. Furthermore, we found out that the extender with 9% LDL concentration significantly enhanced motility, acrosome and plasma membrane integrity of boar sperm after freezing and thawing.

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement under Static Cultivation

( Hai-feng Hu ),( Hai-yan Zhou ),( Xian-lin Wang ),( Yuan-shan Wang ),( Ya-ping Xue ),( Yu-guo Zheng ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H₂O₂ addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H₂O₂, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H₂O₂ 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03- fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

New Insights to the Promoted Bainitic Transformation in Prior Deformed Austenite in a Fe-C-Mn-Si Alloy

Hai-jiang Hu,Guang Xu,Ming-xing Zhou,Qing Yuan 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.2

The varying trends of the amount and rate of bainitic transformation with strains at low temperature were investigated through metallography, X-ray diffraction and dilatometry. The results show that deformation at 573 K promotes bainitic transformation, whereas the promotion degree on bainite transformation by ausforming is nonlinear with strains. The amount of bainite in deformed austenite first increases and then decreases with the increase of strains. There exists a maximum value of the promotion effect corresponding to a critical small strain at a low temperature. Bainitic transformation rate can be increased by ausforming at low temperature, whereas a large strain weakens the acceleration effect. The amount of bainite in deformed materials is synthetically depended on the effect of enhanced nucleation and repressed growth. In addition, the volume fraction of retained austenite is not completely consistent with carbon content, indicating that ausforming plays a important role in determining the amount of austenite.

Cementing failure of the casing-cement-rock interfaces during hydraulic fracturing

Hai Y. Zhu,Jin G. Deng,Jun Zhao,Hu Zhao,Hai L. Liu,Teng Wang 사단법인 한국계산역학회 2014 Computers and Concrete, An International Journal Vol.14 No.1

Using the principle of damage mechanics, zero-thickness pore pressure cohesive elements(PPCE) are used to simulate the casing-cement interface (CCI) and cement-rock interface (CRI). The traction-separation law describes the emergence and propagation of the PPCE. Mohr-coulomb criteria determines the elastic and plastic condition of cement sheath and rock. The finite element model (FEM) of delamination fractures emergence and propagation along the casing-cement-rock (CCR) interfaces during hydraulic fracturing is established, and the emergence and propagation of fractures along the wellbore axial and circumferential direction are simulated. Regadless of the perforation angle (the angle between the perforation and the max. horizontal principle stress), mirco-annulus will be produced alonge the wellbore circumferential direction when the cementation strength of the CCI and the CRI is less than the rock tensile strength; the delamination fractures are hard to propagate along the horizontal wellbore axial direction; emergence and propagation of delamination fractures are most likely produced on the shallow formationwhen the in-situ stresses are lower; the failure mode of cement sheath in the deep well is mainly interfaces seperation and body damange caused by cement expansion and contraction, or pressure testing and well shut-in operations.

Overexpressed Derlin-1 Inhibits ER Expansion in the Endothelial Cells Derived from Human Hepatic Cavernous Hemangioma

Hu, Dong,Ran, Yu-Liang,Zhong, Xing,Hu, Hai,Yu, Long,Lou, Jin-Ning,Sun, Li-Xing,Yang, Zhi-Hua Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6

Proteins that are unfolded or misfolded in the endoplasmic reticulum (ER) must be targeted for refolding or degradation to maintain the homeostasis of the ER. Derlin-1 was reportedly implicated in the retro-translocation of misfolded proteins from the ER to the cytosol for degradation. In this report, we showed that Derlin-1 was down-regulated in the endothelial cells derived from human hepatic cavernous hemangioma (CHEC) compared with other tested cells. Electron microscopy analysis showed that ER was aberrantly enlarged in CHEC cells, but not in other tested cells. When overexpressed, Derlin-1 induced the dilated ER to return normal size. This ER dynamic was associated with the activation of unfolded protein response (UPR). In CHEC cells where Derlin-1 was down-regulated, increased expression of the immunoglobulin heavy chain-binding protein (Bip) and UPR-specific splicing of X-box DNA-binding protein 1 (XBP1) mRNA were detected, as compared with that in other tested cells, indicating that UPR was activated. After Derlin-1 overexpression, the extent of UPR activation diminished, as evidenced by decreased expression of Bip, reduced amount of the spliced form of XBP1 ($XBP1_S$), and elevated expression of the unspliced form of XBP1 ($XBP1_U$). Taken together, these findings provide another example of a single protein being able to affect ER dynamic in mammalian cells, and an insight into the possible molecular mechanism(s).

Hydraulic fracturing experiments of highly deviated well with oriented perforation technique

Zhu, Hai Y.,Deng, Jin G.,Liu, Shu J.,Wen, Min,Peng, Cheng Y.,Li, Ji R.,Chen, Zi J.,Hu, Lian B.,Lin, Hai,Guang, Dong Techno-Press 2014 Geomechanics & engineering Vol.6 No.2

In order to investigate the effect of different perforation angles (the angle between the perforation direction and the maximum horizontal principal stress) on the fracture initiation and propagation during hydraulic fracturing of highly deviated well in oil & gas saturated formation, laboratory experiments of the hydraulic fracturing had been carried out on the basis of non-dimensional similar criteria by using 400^3 $mm^3$ cement cubes. A plane fracture can be produced when the perforations are placed in the direction of the maximum horizontal principal stress. When the perforation angle is $45^{\circ}$, the fractures firstly initiate from the perforations at the upper side of the wellbore, and then turn to the maximum horizontal principal stress direction. When the well deviation angle and perforation angle are both between $45^{\circ}$ and $90^{\circ}$, the fractures hardly initiate from the perforations at the lower side of the wellbore. Well azimuth (the angle between the wellbore axis and the maximum horizontal principal stress) has a little influence on the fracture geometries; however it mainly increases the fracture roughness, fracture continuity and the number of secondary fractures, and also increases the fracture initiation and propagation pressure. Oriented perforating technology should be applied in highly deviated well to obtain a single plane fracture. If the well deviation angle is smaller, the fractures may link up.

Overexpressed Derlin-1 Inhibits ER Expansion in the Endothelial Cells Derived from Human Hepatic Cavernous Hemangioma

( Dong Hu ),( Yu Liang Ran ),( Xing Zhong ),( Hai Hu ),( Long Yu ),( Jin Ning Lou ),( Li Xing Sun ),( Zhi Hua Yang ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.6