Lysine deacetylase inhibition attenuates hypertension and is accompanied by acetylation of mineralocorticoid receptor instead of histone acetylation in spontaneously hypertensive rats

Seok, Y. M.,Lee, H. A.,Park, K. M.,Hwangbo, M. H.,Kim, I. K. Springer Science + Business Media 2016 Naunyn-Schmiedeberg's archives of pharmacology Vol.389 No.7

<P>Inhibition of lysine deacetylase (KDAC) attenuated development of hypertension in spontaneously hypertensive rats (SHRs). We hypothesized that KDAC inhibition attenuates hypertension and is accompanied by acetylation of mineralocorticoid receptors (MR) instead of histone acetylation in SHRs. Valproate (VPA, 0.71 % wt/vol), an inhibitor of class I KDACs, was administered in drinking water to 7-week-old SHRs and Wistar Kyoto rats for 11 weeks. MR acetylation was determined by immunoprecipitation with anti-MR antibody followed by western blot with anti-acetyl-lysine antibody. Expression levels of acetylated histone H3, KDACs, MR target genes, or MR corepressors in the kidney cortex were measured by using western blot analysis or real-time PCR. Recruitment of MR and RNA polymerase II (Pol II) and histone modifications on promoters of target genes were analyzed by performing a chromatin immunoprecipitation (ChIP) assay. Treatment of SHR with VPA increased MR acetylation without affecting MR expression, which attenuated development of hypertension in SHR VPA decreased expression of KDAC class I but globally increased acetylated histone H3. Although VPA treatment increased histone 3 acetylation (H3Ac) and trimethylation of the fourth lysine (H3K4me3) in the promoter regions of MR target genes, it decreased the expression of target genes as well as recruitment of MR and Pol II. These results suggest that KDAC inhibition attenuates the development of hypertension in SHRs and is accompanied by acetylation of MR that is independent of histone acetylation.</P>

Dynamic Mesh 기법을 통한 X-형 제어판 배치 잠수함의 가상구속모형시험

천기현(K.H. Cheon),석우찬(W.C. Seok),박종열(J.Y. Park),서정화(J.H. Seo),이신형(S.H. Rhee) 한국전산유체공학회 2018 한국전산유체공학회지 Vol.23 No.2

X-form configuration of a submarine has advantages of better manoeuvrability than the cruciform configuration, which has been traditionally used. The X-form configuration has a much longer span of control planes than those of the cruciform configuration, so it has the advantage of generating larger control force. Its control system, however, is complex, because horizontal and vertical forces induced by control planes were coupled. For the reason above, its dynamic characteristics should be analysed by identifying a dynamic modelling and deriving hydrodynamic derivatives. The present study carried out for a submarine with an X-form configuration. The test model was BB2, of which design was open most recently by Maritime Research Institute Netherlands. The hydrodynamic force on the BB2 model was estimated using virtual Planar Motion Mechanism test using Computational Fluid Dynamics(CFD). To to this, SNUFOAM, an object-oriented open source CFD tool-kit, was used as a solver for Unsteady Reynolds-Averaged Navier-Stokes simulation. Standard k-ε was selected for turbulence model and dynamic mesh methods was used for dynamic PMM tests.

Upregulation of NAD(P)H:quinone oxidoreductase by radiation potentiates the effect of bioreductive beta-lapachone on cancer cells.

Choi, Eun K,Terai, Kaoru,Ji, In-Mi,Kook, Yeon H,Park, Kyung H,Oh, Eun T,Griffin, Robert J,Lim, Byung U,Kim, Jin-Seok,Lee, Doo S,Boothman, David A,Loren, Melissa,Song, Chang W,Park, Heon Joo Stockton Press 2007 Neoplasia Vol.9 No.8

<P>We found that beta-lapachone (beta-lap), a novel bioreductive drug, caused rapid apoptosis and clonogenic cell death in A549 human lung epithelial cancer cells in vitro in a dose-dependent manner. The clonogenic cell death caused by beta-lap could be significantly inhibited by dicoumarol, an inhibitor of NAD(P)H:quinone oxido-reductase (NQO1), and also by siRNA for NQO1, demonstrating that NQO1-induced bioreduction of beta-lap is an essential step in beta-lap-induced cell death. Irradiation of A549 cells with 4 Gy caused a long-lasting upregulation of NQO1, thereby increasing NQO1-mediated beta-lap-induced cell deaths. Although the direct cause of beta-lap-induced apoptosis is not yet clear, beta-lap treatment reduced the expression of p53 and NF-kappaB, whereas it increased cytochrome C release, caspase-3 activity, and gammaH2AX foci formation. Importantly, beta-lap treatment immediately after irradiation enhanced radiation-induced cell death, indicating that beta-lap sensitizes cancer cells to radiation, in addition to directly killing some of the cells. The growth of A549 tumors induced in immunocompromised mice could be markedly suppressed by local radiation therapy when followed by beta-lap treatment. This is the first study to demonstrate that combined radiotherapy and beta-lap treatment can have a significant effect on human tumor xenografts.</P>

In vivo absorption and disposition of α-cedrene, a sesquiterpene constituent of cedarwood oil, in female and male rats

Kim, T.H.,Yoo, S.D.,Lee, H.S.,Lee, K.M.,Seok, S.H.,Kim, M.G.,Jung, B.H.,Kim, M.G.,Shin, B.S. 日本藥物動態學會 2015 DRUG METABOLISM AND PHARMACOKINETICS Vol.30 No.2

This study aimed to evaluate the potential of α-cedrene as a new anti-obesity drug by characterizing absorption, metabolism and pharmacokinetics in rats. α-Cedrene was administered intravenously (10 and 20 mg/kg) and orally (50 and 100 mg/kg) to female and male Sprague-Dawley rats. Blood, tissues, urine, and feces were collected at predetermined times. α-Cedrene concentrations were determined by a validated gas chromatography-tandem mass spectrometry (GC-MS/MS). A gas chromatography-mass selective detection (GC-MSD) method was used to identify the major metabolite. After i.v. injection, α-cedrene exhibited a rapid clearance (98.4-120.3 ml/min/kg), a large distribution volume (35.9-56.5 l/kg), and a relatively long half-life (4.0-6.4 h). Upon oral administration, it was slowly absorbed (T<SUB>max</SUB> = 4.4 h) with bioavailability of 48.7-84.8%. No gender differences were found in its pharmacokinetics. Upon oral administration, α-cedrene was highly distributed to tissues, with the tissue-to-plasma partition coefficients (K<SUB>p</SUB>) far greater than unity for all tissues. In particular, its distribution to lipid was notably high (K<SUB>p</SUB> = 132.0) compared to other tissues. A mono-hydroxylated metabolite was identified as a preliminary metabolite in rat plasma. These results suggest that α-cedrene has the favorable pharmacokinetic characteristics to be further tested as an anti-obesity drug in clinical studies.

혈중 갑상선자극홀몬 측정의 진단적 의의에 관한 연구

이종석,이학중,문성수,석광호,박우한,한창순 대한핵의학회 1980 핵의학 분자영상 Vol.14 No.2

저자들은 혈중 TSH의 방사면역측정을 정상인과 각종 갑상선질환 환자들에게 실시하여 의미있는 결과를 얻었기에 이에 보고하는 바이다. 환자들은 1978년 1월부터 1980년 8월까지 국립경찰병원 내과에 내원한 각종 갑상선환자 41예와 정상인 22예를 대상으로 하였다. 1) 정상인 22예의 혈충 TSH치는 평균 4.2∼1.7μU/ml이고, 그 범위는 1.9∼7.4μU/ml이었다. 2) 원발성 갑상선기능저하증 9예에 있는 혈중 TSH의 평균치가 97.1±116.4μU/ml이고 그 범위는 14.0±300μU/ml였다. 3) Graves씨병 17예는 혈중 TSH치의 평균이 1.5±0.6μU/ml였다. 4) 갑상선염 8예는 갑상선기능상태에 따라 혈중 TSH치의 변화가 많았으며 그 범위는 1.6∼220μU/ml이었다. 5) 갑상선결절 7예는 혈중 TSH치의 평균이 2.4±2.0μU/ml이었다. 6) Graves씨병에 Taoazole치료후 TSH치가 상승하는 것을 볼 수 있었고, 이것이 병경과를 판정하는데 아주 유용하였다. 이상의 결과로써 혈중 TSH의 측정은 갑상선질환의 진단뿐 아니라 치료경과를 판정하는데도 좋은 수단이 될 수 있다고 하겠다. The radioimmunoassay of TSH (human thyrotropin) was performed by utilizing anti-h-TSH antibody and purified human thyrotropin supplied from Daiichi from Jan. 1978 through Aug. 1980 the serum concentration of TSH was measured on 41 cases with various thyroid diseases, and 22 normal persons. Among 41 cases, 9(22%) were primary hypothyroitlism, 17(41%) Graves' disease, 8(20%) subacute or chronic lymphocytic thyroiditis, and 7(17%) nodular goiter. The results were as follows:1) The normal values of serum TSH in 22 cases of control group were 4.2±1.7μU/ml, (1.9∼7.4μU/ml), which were within normal range in kit used in this study. 2) The serum TSH concentration in 9 cases with primary hypoehroidism were 97.1±116.4μU/ml(14. 0∼300μU/ml), which were significantly elevated as compared, with normal control values. 3) The serum TSH concentration in 17 cases with Graves' disease were 1.5±0.6μU/ml(1.0∼2.5μU/ml), which were below than normal control. 4) The serum TSH concentration in 8 cases with subacute or chrotic thyroiditis revealed wide ranges(1.6∼220μU/ml), according to the state of thyroid function. 5) The serum TSH values in 7 cases with nodular goiters were 2.3±2.0μU/ml, which were strictly within normal levels. 6) The serum TSH levels were elevated during prolonged treatment with Tapazole(Methimazole) without serial check of the serum TSH concentration in Graves' disease, so the serial measurement of serum TSH concentration was considered of available index of thyroid states.

초음파 측정에 의한 한우의 도체육량 예측

이용준,전기준,최성복,석홍기,김석중,이성기,송영한 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.2

본 시험은 초음파를 이용한 한우의 도체육량등급 예측율을 향상시키기 위하여 24개월령 거세한우 573두를 대상으로 출하직전에 체중측정과 함께 실시간 초음파 진단기를 이용하여 도형체질을 측정하였다. 초음파 측정치와 도체실측치와의 비교분석에서 도출된 다양한 육량예측 기법을 이용하여 도체육량등급을 예측하였고, 그 결과를 요약하면 다음과 같다. 등지방 두께와 배최장근 단면적의 도체 실측치와 초음파 측정치간의 차이는 각각 0.6±1.65mm와 0.7±5.56㎡로 나타났다. 등지방 두께 및 배최장근 단면적의 초음파 측정치와 도체 실측치간의 상관계수는 각각 0.86 및 0.82로 높게 나타났다(p<0.001). 도체육량등급 예측율은 육량지수 산식, 등지방 보정식, 중회귀 분석 및 의사결정나무 분석에서 각각 80.3%, 81.3%, 80.1% 및 81.8%를 보여, 의사결정나무 분석이 예측율과 활용성이 가장 높은 것으로 나타났다. This study was conducted to predict the carcass yield traits using ultrasound before slaughter and to enhance the prediction accuracy of carcass yield grade by applying various strategies. For this experiment, five hundred seventy three Hanwoo steers of 24 months of age were used. Difference between ultrasound results and carcass measure of BFT and LMA was 0.6±1.65㎜ and 0.7±5.56㎤, respectively. Correlation coefficient between ultrasound result and carcass measure of BFT and LMA was 0.86 and 0.82 respectively (p<0.001). Results for improving predictions of yield grade by four methods0the Korean yield grade index equation, fat depth alone, regression and decision tree methods were 80.3%, 81.3% 80.1% and 81.8%, respectively. We conclude that the decision tree method can easily predict yield grade and is also useful for increasing prediction accuracy rate.

장티푸스의 Cefotaxime의 치료효과

박승철,이미안,최혜란,최한상,김성윤,정선근,박찬현,김태화,석성억,이창홍 대한감염학회 1984 감염 Vol.16 No.1

Cefotaxime은 반합성 Cephalosporin 제제로서 광범위항생제이며 β-lactamase에 안정성을 갖고 있으며 S. typhi에 대해서는 최저저지농도가 0.04㎍/dl로서 매우 유요한 것으로 나타나 있다. Mekendrik은 2예의 장티푸스환자에서 Cefotaxime 4.0gm/day 치료로 임상적인 효과를 못보았다고 한다. 그 이유는 Cefotaxime이 포유동물의 세포내로 침투하기 어려운 때문이라고 하였으며 또다른 임상연구에서는 Cefotaxime 투여 24시간후 혈액배양에서 S. typhi가 자란다고 한다. 본 연구에서는 45명의 많은 환자에서 Cefotaxime을 투여한 결과 비교적 양호한 치료효과를 얻었다. Table2에서 보는 바와 같이 45명중 31명이 7일이내에 치료 효과를 보였다. 평균 치료기간이 6.9일로서 Chloram-phenicol 투여시의 5.4일 보다는 길으나 임상적으로는 치료제로서 사용할만한 약제로 볼 수 있다. Santiago 에서의 연구결과는 Chloramphenicol 5.1일, Ampicillin은 6.7일이었으며 Co-trimoxazole은 7일이었다. 31명의 환자에서 Cefotaxime 2.0 gm/day의 용량으로는 해열이 되지않으므로 1 일 4.0 gm으로 중량하여 평균 3.2일만에 해열이 되었다. 그러나 초기치료부터 1일 4.0 gm 으로 시작한 자료가 없으므로 용량간에 효과를 비교할만한 근거는 없다. 3예의 재발과 5예의 단기보균자수는 Amoxycillin 치료시와 유사하다. 이들은 Co-trinoxazole로 치료하였다. 3예의 장출혈환자는 Cefortaxime의 부작용은 아니며 장티푸스환자에서 통상적으로 볼 수 있는 합병증이라고 사료된다. Cefotaxime 투여시 약간의 피부발진 이외의 부작용을 발견할 수 없었다. Cefotaxime은 가격이 비싸고 경구적 투여라는 단점이 있으나 골수장애를 일으킬 수 있는 Chloramphenicol에 대신해서 장티푸스치료에 사용할 수 있는 약제다.

철강산업 용융로의 대기오염물질 배출계수 산정 연구

석광설,방선애,홍지형,이석조,김대곤,이대균,허정숙,이은정 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.4

The purpose of this study is 10 estimate of emission factors of the air pollutants for the melting furnaces for the iron and steel industry. The result of this study is able to obtain the emission factor of particulate matters (PM), sulfur dioxide, nitrogen oxides for melting furnace. The emission factors of each pollutants were as follows: - the emission factor varied between 6.13E-03 ∼ 6.12E-01kg/ton for PM - 1.59E-01 ∼ 2.45E+00kg/ton for S0₂ - 6.82E-02 ∼ 6.88E-01kg/ton for NOx, respectively. Analysis of the differences in the emission factors of ours and U.S. EPA's yielded the following results for the Wilcoxon method: p>0.05. The statistical analysis showed no differences in the our emission factors and U.S. EPA's

마이크로밀링에서 피에조 구동기의 전압제어를 이용한 원주가공의 성능향상

석진우,정병묵,고태조,김희술,박종권 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

Recently, there are many studies for the micro-machining using Piezo actuator. However, because of its step by step motion, it is nearly impossible to increase the machining accuracy for a circular path. To increase the accuracy, it is well known that it is necessary the finer and synchronous movement for x-y axes. Therefore, this paper proposes a voltage control for finer movement of the actuator, and realizes a synchronous control for the x-y axes. The experimental results show that the machining accuracy is remarkably improved.

Transparent superhydrophobic surface by silicone oil combustion

Seo, K.,Kim, M.,Seok, S.,Kim, D.H. Elsevier 2016 Colloids and surfaces. A, Physicochemical and engi Vol.492 No.-

<P>A transparent superhydrophobic coating can be easily created through the use of commercial silicone oil and controlled combustion. In this simple fabrication, silicone oil is sprayed onto a hot glass heated to about 550 degrees C, resulting in the transparent superhydrophobic coating on the glass. The coating is stable at temperature up to about 450 degrees C, and against a saline solution and acidic or basic solutions with pH from 4 to 10. This silicone oil-based process does not require any additional solvent, further surface treatment, drying process or post-treatment process. Several applications of this process are exemplified through the proof-of-concept demonstrations. (C) 2015 Elsevier B.V. All rights reserved.</P>