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한국 달맞이꽃의 Isozyme Analysis 및 Fatty Acid Composition에 관한 연구
허홍욱,허경혜 釜山大學校 師範大學 1993 교사교육연구 Vol.27 No.-
The genetic variation in the Korean populations of Everning Primrose, Oenothera odorata L., was examined to check out the frequency of allozyme variation. The allozyme variation of ADH, MDH, 6-PGDH, PGM, and IDH were assayed by means of starh gel electrophoresis. The ADH, 6-PGDH and PGM loci were almost monomorphic which was under control of one locus. Only Seol-ag population shows slow allele(3.0%) at the ADH-locus. At the MDH-2 locus, the frequency of slow and fast allele were 5.9% and 4.5% on the average, this represented a single locus with two alleles for a dimeric enzyme. And also IDH-locus were 0.7% and 3.6% respectively. Fatty acid comosition were investigated by means of capillary column gas-chromatography. The fatty acid of Everning Primrose oil was composed of palmitic(5.79-7.48), stearic (1.51-1.79%), oleic(4.15-7.81%), and linoleic(71.37-76.38%) acid. Particularly, GLA which is a precursor of prostaglandin was 10.15-12.51% among total fatty acid.
Genetic Studies of Oenothera odorata Populations in Korea Based on Isozyme Analysis
Huh, Hong Wook,Huh, Gyung Hye,Huh, Man Kyu 한국식물학회 1996 Journal of Plant Biology Vol.39 No.3
The genetic variation in Korean evening primrose (Oenothera odorata L.) populations was examined to estimate the level of allozyme variation within populations using starch gel electrophoresis. 7 of 13 loci (Adh, Est-1, Est-2, Mdh-2, Pgd-2, Pgm-1, and Idh) revealed (Ps=43.2%) were polymorphic. The mean number of alleles per locus (A) and polymorphic locus (Ap) for populations were 1.64 and 2.46, respectively. The effective number of alleles (Aep) within populations relatively was low ranging from 1.08 to 1.22 with a mean of 1.14. Within populations, the mean number of allele per polymorphic loci (Ap) was 2.46, the mean number of alleles per locus (A) was 1.64, and the mean genetic diversity was 0.093. About 2.7% of the total allozyme diversity resided among populations (Mean G_ST=0.0274). F_ts, a measure of the deviation from random mating within 13 populations, was relative low (mean F_ts=-0.03636). The indirect estimate of gene flow, based on the mean G_ST, was high (Nm=8.88). Estimates of gene flow were consistent with low levels of genetic differentiation among populations.
Kim, Young-Hwa,Huh, Gyung Hye The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.1
Ran is a small GTP-binding protein that binds and subsequently hydrolyzes GTP. The functions of Ran in nuclear transport and mitotic progression are well conserved in plants and animals. In animal cells, stress treatments cause Ran relocalization and slowing of nuclear transport, but the role of Ran proteins in plant cells exposed to stress is still unclear. We have therefore compared Ran genes from three EST libraries construed from different cell types of sweetpotato and the distribution pattern of Ran ESTs differed according to cell type. We further characterized two IbRan genes. IbRan1 is a specific EST to the suspension cells and leaf libraries, and IbRan2 is specific EST to the root library. IbRan1 showed 94.6 % identity with IbRan2 at the amino acid level, but the C-terminal region of IbRan1 differed from that of IbRan2. These two genes showed tissue-specific differential regulation in wounded tissues. Chilling stress induced a similar expression pattern in both IbRan genes in the leaves and petioles, but they were differently regulated in the roots. Hydrogen peroxide treatment highly stimulated IbRan2 mRNA expression in the leaves and petioles, but had no significant effect on IbRan1 gene expression. These results showed that the transcription of these two IbRan genes responds differentially to abiotic stresses and that they are subjected to tissue-specific regulation. Plant Ran-type small G-proteins are a multigenic family, and the characterization of each Ran genes under various environmental stresses will contribute toward our understanding of the distinctive function of each plant Ran isoform.
Regulation of Cinnamyl Alcohol Dehydrogenase (CAD) Gene Family in Lignin Biosynthesis
Young-Hwa Kim(김영화),Gyung-Hye Huh(허경혜) 한국생명과학회 2021 생명과학회지 Vol.31 No.10
리그닌은 식물의 세포벽에 풍부하게 존재하는 복잡한 phenylpropanoid 중합체이다. 주로 물 수송과 기계적 강도를 유지하는 조직에 존재하며 수분을 운반하거나, 기계적인 지지를 담당한다. 또한, 리그닌은 병원균의 감염이나 상처에 대한 물리적인 장벽으로 작용함으로써 방어 기작에 관여한다. 리그닌을 생성하는 모노리그놀 전구체는 cinnamyl alcohol dehydrogenase (CAD) 유전자에 의해 합성된다. CAD는 cinnamaldehyde를 cinnamyl alcohol(p-coumaryl, coniferyl, sinapyl alcohol)로 전환하는 효소이다. CAD는 속씨식물에서 multigenic family로 존재하며 여러 식물 종에서 다른 기능을 가진 CAD isoform이 밝혀졌다. CAD 유전자의 여러 isoform은 식물의 발달 및 환경 신호에 따라 다르게 발현되었다. 하나의 isoform이 발달 리그닌화에 관여하는 반면, 다른 isoform은 방어 리그닌 및 기타 세포벽에 결합된 페놀의 구성에 영향을 미칠 수 있음을 보여주었다. CAD isoform에 따라 기질 특이성이 다르게 나타나고, 이는 리그닌 합성을 조절하는 CAD 단백질의 생화학적 특성을 나타내는데 기여한다. 본 논문에서는 리그닌 생합성에서 CAD multigenic family 유전자의 발현과 조절에 대하여 설명하였다. CAD multigenic family의 isoform들은 유전적 조절이 복잡하고, 식물 발달 과정의 신호 경로와 스트레스 반응이 밀접하게 연동되어 있다. CAD 유전자에 의한 모노리그놀 합성은 발달 및 환경 신호에 의해 조절될 가능성이 높다. Lignin is a complex phenylpropanoid polymer abundant in the cell walls of vascular plants. It is mainly presented in conducting and supporting tissues, assisting in water transport and mechanical strength. Lignification is also utilized as a defense mechanism against pathogen infection or wounding to protect plant tissues. The monolignol precursors of lignin are synthesized by cinnamyl alcohol dehydrogenase (CAD). CAD catalyzes cinnamaldehydes to cinnamyl alcohols, such as p-coumaryl, coniferyl, and sinapyl alcohols. CAD exists as a multigenic family in angiosperms, and CAD isoforms with different functions have been identified in different plant species. Multiple isoforms of CAD genes are differentially expressed during development and upon environmental cues. CAD enzymes having different functions have been found so far, showing that one of its isoforms may be involved in developmental lignification, whereas others may affect the composition of defensive lignins and other wall-bound phenolics. Substrate specificity appears differently depending on the CAD isoform, which contributes to revealing the biochemical properties of CAD proteins that regulate lignin synthesis. In this review, details regarding the expression and regulation of the CAD family in lignin biosynthesis are discussed. The isoforms of the CAD multigenic family have complex genetic regulation, and the signaling pathway and stress responses of plant development are closely linked. The synthesis of monolignol by CAD genes is likely to be regulated by development and environmental cues as well.