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( Gil Myeong Seong ),( Jae Chun Lee ),( Jong Hoo Lee ),( Jeong Hong Kim ),( Mi Ok Kim ) 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.76 No.4
Background: Mycobacterial identification in active pulmonary tuberculosis (APTB) is confirmative, even though successful rates using self-expectorated sputum are limited. Sputum specimens collected by hypertonic saline nebulization showed higher bacteriologic diagnostic sensitivities over those of self-expectoration, mostly studied in smear-negative or sputum-scarce patients. The efficacy of induced sputum was rarely assessed in real clinical settings. Methods: A prospective randomized case-control study was performed in one hospital. The subjects highly suspicious of APTB were asked to provide 3 pairs of sputum specimens in 3 consecutive days. The first pairs of the specimens were obtained either by self-expectoration (ES) from the next day of the visit or sputum induction with 7% saline nebulization in clinic (SI), and the other specimens were collected in the same way. The samples were tested in microscopy, culture, and polymerase chain reaction (PCR). The outcomes of the bacteriological diagnosis were compared. Results: Seventy six patients were assigned to either ES (38 subjects, median age of 51, 65.8% male) or SI (38 subjects, median age of 55, 52.6% male). APTB was clinically confirmed in 51 patients (70.8%), 27 in ES and 24 in SI. Among the APTB, more adequate specimens were collected from SI (41/65, 63.1%) than ES (34/80, 42.5%) (p=0.01). Bacteriological confirmation was achieved in 14 (58.3%) patients in SI, and 13 (48.1%) in ES (p=0.46). In the same-day bacteriological diagnosis with microscopy and PCR, there were positive results for 9 patients (37.5%) in SI and 7 patients (25.9%) in ES (p=0.37). Conclusion: Sputum induction improves sputum specimen adequacy. It may be useful for the same-day bacteriological diagnosis with microscopic examination and PCR.
Seong, Gil Myeong,Lee, Jaechun,Lee, Jong Hoo,Kim, Jeong Hong,Kim, Miok The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.76 No.4
Background: Mycobacterial identification in active pulmonary tuberculosis (APTB) is confirmative, even though successful rates using self-expectorated sputum are limited. Sputum specimens collected by hypertonic saline nebulization showed higher bacteriologic diagnostic sensitivities over those of self-expectoration, mostly studied in smear-negative or sputum-scarce patients. The efficacy of induced sputum was rarely assessed in real clinical settings. Methods: A prospective randomized case-control study was performed in one hospital. The subjects highly suspicious of APTB were asked to provide 3 pairs of sputum specimens in 3 consecutive days. The first pairs of the specimens were obtained either by self-expectoration (ES) from the next day of the visit or sputum induction with 7% saline nebulization in clinic (SI), and the other specimens were collected in the same way. The samples were tested in microscopy, culture, and polymerase chain reaction (PCR). The outcomes of the bacteriological diagnosis were compared. Results: Seventy six patients were assigned to either ES (38 subjects, median age of 51, 65.8% male) or SI (38 subjects, median age of 55, 52.6% male). APTB was clinically confirmed in 51 patients (70.8%), 27 in ES and 24 in SI. Among the APTB, more adequate specimens were collected from SI (41/65, 63.1%) than ES (34/80, 42.5%) (p=0.01). Bacteriological confirmation was achieved in 14 (58.3%) patients in SI, and 13 (48.1%) in ES (p=0.46). In the same-day bacteriological diagnosis with microscopy and PCR, there were positive results for 9 patients (37.5%) in SI and 7 patients (25.9%) in ES (p=0.37). Conclusion: Sputum induction improves sputum specimen adequacy. It may be useful for the same-day bacteriological diagnosis with microscopic examination and PCR.
Myeong Seok Kim,Ho Sub An,Gil Ja Kim,Yong Soon Kim,Gyung Choi,Heung Gyu Park,Hyun Woo Kim,Seong Il Kim,Young Guk Kim,Chun Geun Park,Jae Gi Chang 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05
Background : The purpose of this study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Angelica acutiloba K. And it accomplished one’s purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm’s income. Methods and Results : Method of fertilization application were conducted under four condition compose to standard application (N-P-K-Compost: fermented mixed oil cake fertilizer applied at 14-10-10-600 ㎏․10 a−1cultivation. Compost and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application (N-K) applied at 100%, 50%, 40%, 30% of basal fertilizer in April 14, 50%, 40% and 30% of top dressing were two, three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Sowing dates with Jangsung varieties in April 28 of the year 2015. Planting density were spaced 30 ㎝ apart in rows 15 ㎝ apart with black color non-woven cloth covering open cultivation by level row 90 ㎝. Plot design randomized block 3 repetition. Conclusion : Growth characteristics by 50%, 70% and 60% of top dressing were two, three times application (N-K) in June 1, July 15, August 15 compared to standard application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of leaf and main root of aboveground, subterranean part growth increment rising highest. Dried roots yields compared to standard fertilizer 100%application (217 kg․10 a−1) treatment. 50%, 70% and 60% of top dressing were two, three times application (N-K) increased by 6%, 14%, and 18%. respectively.