Poster Session 1 : Oral Presentation ; Metabolism and Enzymology : Development of cosmeceutical cosmetics using enzyme bio-conversion system

( Ghang Tai Lee ),( Ji Youn Kwon ),( Dong Jun Bae ),( Yun Ju Jung ),( Myoung Hee Lee ),( Sei Ryang Oh ),( Dong Il Jang ) 한국생화학분자생물학회 (구 한국생화학회) 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-

Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes

LEE, GHANG TAI,CHA, HWA JUN,LEE, KWANG SIK,LEE, KUN KOOK,HONG, JIN TAE,AHN, KYU JOONG,AN, IN-SOOK,AN, SUNGKWAN,BAE, SEUNGHEE UNKNOWN 2014 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.33 No.3

Ultraviolet (UV) radiation induces severe alterations in the molecular and cellular components of normal human dermal fibroblast (NHDF) cells by disrupting many intracellular transduction cascades. Although UV responses have been well documented at the genome and proteome levels, UV protective effects have not been elucidated at these levels. The aim of the present study was to demonstrate that arctiin, a phytochemical isolated from the plant Arctium lappa, induced a protective effect against UVB radiation by changing microRNA (miRNA) expression profiles. Using flow cytometry, and water-soluble tetrazolium salt (WST-1)-based cell viability, wound healing, and DNA repair assays we showed that pretreatment with arctiin prior to UVB irradiation reduced UVB-induced apoptosis, cell migration defects, and DNA damage in NHDF cells. It was also found that arctiin-induced UVB protection is associated with altered miRNA expression profiles. Bioinformatic analysis revealed that the deregulated miRNAs were functionally involved in mitogen-activated protein kinase (MAPK) signaling and cancer signaling pathways. The results suggest that arctiin acts as a UVB protective agent by altering specific miRNA expression in NHDF cells.

Inhibitory Effects of Ramulus Mori Extracts on Melanogenesis

( Ghang-tai Lee ),( Bong-soo Shin ),( Beom-jun Kim ),( Jeong-ha Kim ),( Byoung-kee Jo ) 대한화장품학회 1997 대한화장품학회지 Vol.23 No.2

It has been observed that local increase in melanin synthesis or uneven distribution can cause local hyperpigmentation or spot. Pigmentary disorders are caused by various factors, including inflammation, imbalance of hormones, and genetic disorder[1]. Recently the harmfulness of Ultraviolet(UV) radiation is increasing due to destruction of ozone layer. Excessive exposure to UV radiation caused post-inflammatory pigmentation[2,3]. Most women want to avoid uneven skin pigmentation. To satisfy this desire many cosmetic companies have been developing melanogenesis inhibitors and finding promising active agents for use in cosmetic preparations for skin whitening. In cosmetic preparations, many inhibitors such as kojic acid[4], arbutin[5], ascorbic acid, and licorice extracts6 have been used as whitening purpose. Plant extracts having an inhibitory effect on melanin formation may be a good choice for cosmetic purpose because of their relatively lower side effects. Therefore, we screened 285 plant extracts for their inhibitory activity in tyrosinase[7]. Of the plant extracts, ramulus mori(the young twig of Morus alba L.) extracts showed potent tyrosinase inhibition activity. We also identified the active compound in the extract.

Poster Session 1 : Development of cosmeceutical cosmetics using enzyme bio-conversion system

( Ghang Tai Lee ),( Ji Youn Kwon ),( Dong Jun Bae ),( Yun Ju Jung ),( Myoung Hee Lee ),( Sei Ryang Oh ),( Dong Il Jang ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

효소 생전환 시스템을 이용한 기능성 화장품 개발

이강태 ( Ghang Tai Lee ),권지연 ( Ji Youn Kwon ),배동준 ( Dong Jun Bae ),유창선 ( Chang Seon Yu ),이명희 ( Myoung Hee Lee ),오세량 ( Sei Ryang Oh ),장동일 ( Dong Il Jang ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.1

본 논문은 효소 생전환 시스템을 적용, 안정한 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA2G)를 ascorbic acid (AA)와 glucose로 분해하여 이 물질의 피부에서의 효과를 분석한 것에 관한 것이다. 일반적으로 AA는 높은 환원력을 가지고 있으며, 화장품에서 다양한 용도로 사용할 수 있지만 수용액 상에서 불안정하기 때문에 사용에 제약이 많이 있다. AA2G는 AA를 안정화시킨 미백 기능성 원료이지만 AA와 비교해서 효과가 낮은 것으로 알려져 있다. 이에 본 연구자들은 AA2G의 안정성과 AA의 효과를 동시에 갖는 효소 생전환 시스템을 이용한 기능성 제품을 개발하였다. AA2G는 당 분해 효소인 amigase에 의해 30min 경과 후에 약 80%가 AA로 전환되며 이 시스템의 미백 효과를 측정한 결과 같은 농도의 AA와 비교해 유사한 티로시나제 활성 억제 효과가 나타나는 것으로 확인되었다(AA2G는 타이로시나아제 활성 억제 효과가 없음). 또한 AA2G와 amigase를 함유한 제형은 인공 색소 침착 후의 미백 효과 시험에서 AA2G 만을 함유한 제형과 비교하여 높은 미백효과가 나타나는 것으로 확인되었다. 이와 같은 효소 생전환 시스템은 불안정한 AA를 보다 효과적으로 피부에 적용할 수 있는 새로운 방법이라 할 수 있다. This study is about the cosmeceutical products using enzyme induced bio-conversion system. In general, ascorbic acid (AA) has the higher reducing activity and can be used for various purpose in the cosmetics. But it is very unstable in the aqueous system and difficult to maintain its stability in the cosmetics product. 2-O-α-D-Glucopyranosyl-L-ascorbic acid (AA2G) is the stabilized form of AA and showed the less whitening activity than AA. In this study, we developed bio-conversion system improving the stability and efficacy of AA2G and AA, respectively. In this system, AA2G (over 80%) can be converted to AA and glucose within 30 min. The converted product showed higher anti-tyrosinase activity like AA (AA2G showed no anti tyrosinase activity) and depigmenting activity in the artificial tanning test. From these results, we could conclude this system is a brand new method to increase the activity of AA and maintain its stability.

天精氣保丹의 자외선에 의한 세포 손상 억제 효과

이강태(Ghang Tai Lee),박시준(Si Jun Park),이정로(Jung No Lee),이광식(Kwang Sik Lee),김대성(Dae Sung Kim),문연자(YeunJa-Mun),이건국(Kun Kuk Lee),우원홍(Won Hong Woo) 한의병리학회 2010 동의생리병리학회지 Vol.24 No.6

천연한방소재인 연교 추출물의 미백 효과에 관한 연구

이정노 ( Jung Noh Lee ),박재희 ( Jea Hee Park ),김상우 ( Sang Woo Kim ),유영경 ( Young Kyoung Yoo ),이강태 ( Ghang Tai Lee ),이건국 ( Kun Kook Lee ) 대한화장품학회 2007 대한화장품학회지 Vol.33 No.2

본 연구는 연교 추출물과 연교 추출물에서 분리한 활성물질의 멜라닌 생성 억제 효과에 관한 것이다. 연교 추출물은 0.1~1.0% (v/v)에서 세포독성이 없는 것을 확인하였으며 이를 바탕으로 멜라닌 생성억제 실험결과 1.0 %에서 63.1±3.1% 저해 효과를 확인하였다. 또한 활성물질을 분리하여 ¹H-NMR, ¹³C-NMR, Mass analysis 등의 기기분석 결과 리그난 계열의 활성물질인 4-[(3,4-Dimethoxyphenyl)methyl)]dihydro-3-[(4-hydroxy-3-methoxyphenyl)methyl]-2(3H)-furanone (arctigenin)으로 동정되었다. 활성물질에 대한 멜라닌 저해효과를 확인한 결과 농도 의존적으로 3.0 μg/mL에서 52.7±3.1%를 저해하는 것을 확인하였다. 또한 연교 추출물을 3 % 함유한 크림의 미백효과에 대한 임상실험을 진행한 결과 전문의의 육안평가와 기기평가 모두 통계적으로 유의한 결과를 얻을 수 있었다. 결론적으로 연교 추출물은 미백 효능을 갖는 화장품 소재로서의 개발 가능성이 클 것으로 기대된다. The aim of this study was to investigate the inhibitory effects of Forsythia suspensa fruit extracts (FSfE) (0.1~1.0%) and their active component on melanogenesis. FSfE dose-dependently inhibited melanin synthesis (up to 63.1±3.1% at the concentration of 1.0%) without cell cytotoxicity. We purifed one active compound from FSfE and identified its structure. It was identified as 4-[(3,4-Dimethoxyphenyl)methyl)]dihydro-3-[(4-hydroxy-3-methoxyphenyl)methyl]-2(3H)-furanone (arctigenin) by ¹H-NMR, ¹³C-NMR, and Mass analysis. Arctigenin also inhibited melanin synthesis in a dose dependent manner (up to 52.7±3.1% at the concentration of 3.0 μg/mL). In order to verify the whitening activity of the cream containing 3% FSfE, we performed the clinical test with twenty five female volunteers for 8 weeks. Bioengineering analyses and visual assessment by doctors at the initial time point and 8 weeks after applications showed that cream containing FSfE have significant whiteining effect (paired t-test). From the results, we conclude that the FSfE can be used as a useful whitening agent.

노니 추출물의 주름개선 효과연구

이정노 ( Jung Noh Lee ),김상우 ( Sang Woo Kim ),유영경 ( Young Kyoung Yoo ),이강태 ( Ghang Tai Lee ),이건국 ( Kun Kook Lee ) 대한화장품학회 2006 대한화장품학회지 Vol.32 No.4

주름생성의 주요 원인은 자외선, 공기 오염, 담배 연기, 스트레스 등을 들 수 있다. 특히, 자외선은 피부에 다양한 형태로 영향을 주어 깊은 주름, 잔주름, 피부거침, 피부건조와 같은 현상을 발생시켜 피부노화를 유발시킨다. 이러한 주름 생성 해결 및 피부 노화 문제의 해결은 화장품에서 가장 활발하게 연구되는 분야 중 하나이다. 최근에 열대 식물인 노니로부터 추출한 유효성분이 주름 개선에서 가장 중요한 콜라겐 생합성을 촉진하는 효과를 확인하였으며, 특히 노니 추출물로부터 분리한 스코폴레틴(scopoletin)이 이러한 효과를 주는 활성 성분임을 확인하였다. 스코폴레틴은 인체 섬유아 세포를 이용한 콜라겐 합성 촉진 효과 시험에서 농도 의존적으로 콜라겐 합성을 촉진하는 활성을 보였다(0.2 μg/mL-89.5% 콜라겐 생합성 촉진. 또한, 주름 개선 효과를 확인하기 위해 3% (v/v) 노니 추출물을 함유한 크림을 이용하여 12주 동안 임상연구를 수행하여, 안면의 주름 감소 정도를 측정하였으며 이 제품이 주름을 감소시켜주는 뛰어난 효과가 있다는 것을 확인하였다. 이러한 결과들은 노니 추출물이 주름 개선효과가 매우 높으며, 노화 방지 원료로서 이용 가능성이 매우 높다는 것을 보여주는 것이라 할 수 있다. Wrinkle formation is mainly attributed to the environmental factors such as UV rays, air pollution, smoking and stress etc. Especially, UV rays induce premature skin aging which is characterized by deep wrinkle, leathery dryness etc. Recently, researches on the wrinkle formation and its prevention have been the main theme in cosmetics fields. We have studied the various plant extracts having anti-wrinkle effects and finally showed that Noni (Morinda citrifolia) extracts have the efficacy of promoting the type I collagen synthesis in normal human fibroblast, using PICP assay. We purified one active compound from Noni extracts and identified its structure. It was identified as 6,7-Dimethoxy-2H-1-Benzopyran-2-one; scopoletin by ¹H-NMR, ¹³C-NMR, IR, Mass analysis. Scopoletin increased collagen synthesis in a dose dependent manner (89.5% at 0.2 μg/mL). In order to verify the anti-aging effectiveness of the cream containing 3% noni extracsts, we performed the in vivo test with some female volunteers for 12 weeks. It reduced the signs of aging, especially face wrinkles. From these results, we conclude that the noni extracts could be used as an useful anti-wrinkle agent.

항균활성을 갖는 천연물 개발과 화장품 응용에 관한 연구

이지영 ( Ji Young Lee ),이정노 ( Jung No Lee ),이강태 ( Ghang Tai Lee ),이건국 ( Kun Kook Lee ) 대한화장품학회 2012 대한화장품학회지 Vol.38 No.2

본 연구는 항균 활성을 지니는 식물추출물을 찾고 화장품에서 방부제로서 적용하기 위해 진행되었다. 디스크 확산법(disk diffusion method)을 통해 3가지 식물 추출물, 함박꽃나무(천녀목란, Magnolia sieboldii), 오배자(Rhus chinensis), 메타세콰이어(Metasequioa glyptostroboides)가 항균활성을 지니고 있음을 확인하였다. 세 물질의 최소저해농도(MIC)를 측정한 결과 메타세콰이어는 0.3 ∼ 0.35 %, 함박꽃나무는 0.35 ∼ 0.4 % 농도에서 곰팡이의 생장을 억제하고 오배자는 0.45 ∼ 0.5 % 농도에서 세균의 생장을 억제함을 확인하였다. 또한 추출물 내의 항균 활성을 지니는 성분을 분리하여 분석한 결과 메타세콰이어에서 분리한 caryophyllene oxide와 caryophyllene, 함박꽃나무에서 분리한 costunolide와 dehydrocostus lactone, 오배자에서 분리한 ethyl gallate, ethyl-3-gallate 등이 항균활성을 지닌 물질임을 확인하였다. O/W 에멀션 제형에서 식물 추출물을 넣고 방부력을 확인한 결과 혼합사용 시 세균과 곰팡이 모두에 대한 방부효과가 있음을 확인하였다. 따라서 함박꽃나무, 오배자, 메타세콰이어 추출물의 혼합물은 기존의 화학 방부제를 대체할 천연 방부제로서 화장품에서 응용할 수 있을 것으로 기대된다. This study is focused on finding new natural materials that have antimicrobial activity. We found that 3 plants extracts, Magnolia sieboldii K. Koch (M. sieboldii K. Koch), Rhus chinensis (R. chinensis) and Metasequioa glyptostroboides (M. glyptostroboides), have antimicrobial activities by disk diffusion method. We determined the Minimum Inhibitory Concentation (MIC) of each and found that 0.3 ∼ 0.35 % of M. glyptostroboides essential oil and 0.35 ∼ 0.4 % of M. sieboldii K. Koch extracts inhibited fungal growth and 0.45 ∼ 0.5 % of R. chinensis extracts inhibited bacterial growth. We isolated compounds from extracts and verified what have antimicrobial activity. As a result we found that caryophyllene oxide and caryophyllene isolated from M. glyptostroboides, costunolide and dehydrocostus lactone from M. sieboldii K. Koch and ethyl gallate, ethyl-3-gallate from R. chinensis have antimicrobial activities. In accordance with antimicrobial activity, O/W cosmetic emulsion containing mixture of 3 plants extracts showed preservative efficacy against both bacteria and fungi. Based on the above data we suggest that extracts from M. siebodii K. Koch, R. chinensis, and M. glyptostroboides, replace chemical synthetic preservatives and be applied as a natural preservative.

Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

LIM, KYUNG MI,AN, SUNGKWAN,LEE, OK-KYU,LEE, MYUNG JOO,LEE, JEONG PYO,LEE, KWANG SIK,LEE, GHANG TAI,LEE, KUN KOOK,BAE, SEUNGHEE SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.2

<P>Dermal papilla (DP) cells function as important regulators of the hair growth cycle. The loss of these cells is a primary cause of diseases characterized by hair loss, including alopecia, and evidence has revealed significantly increased levels of reactive oxygen species (ROS) in hair tissue and DP cells in the balding population. In the present study, troxerutin, a flavonoid derivative of rutin, was demonstrated to have a protective effect against H<SUB>2</SUB>O<SUB>2</SUB>-mediated cellular damage in human DP (HDP) cells. Biochemical assays revealed that pretreatment with troxerutin exerted a protective effect against H<SUB>2</SUB>O<SUB>2</SUB>-induced loss of cell viability and H<SUB>2</SUB>O<SUB>2</SUB> induced cell death. Further experiments confirmed that troxerutin inhibited the H<SUB>2</SUB>O<SUB>2</SUB>-induced production of ROS and upregulation of senescence-associated β-galactosidase activity. Using microRNA (miRNA) microarrays, the present study identified 24 miRNAs, which were differentially expressed in the troxerutin pretreated, H<SUB>2</SUB>O<SUB>2</SUB>-treated HDP cells. Subsequent prediction using bioinformatics analysis revealed that the altered miRNAs were functionally involved in several cell signaling pathways, including the mitogen-activated protein kinase and WNT pathways. Overall, these results indicated that ROS-mediated cellular damage was inhibited by troxerutin and suggested that the use of troxerutin may be an effective approach in the treatment of alopecia.</P>