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( Gyong Moon Kim ),( Han Mi Jung ),( Ju Hee Lee ),( En Ah Cho ),( Young Min Park ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: The concept of induced pluripotent stem cells ( IPS) might suggest that genetically programmed or modified somatic cells such as IPS from somatic cells or somatic cells themselves as lipocytes, fibroblasts could be another source of cell therapy, especially in the poor diabetic wound healing. Objectives: The aim of this study was to evaluate the effectiveness about the autologous fibroblasts of diabetic mouse themselves as a alternatives of complex stem cell transplantation therapy. Methods: We chose diabetic wound model as a chronic nonhealing wound. We sampled and cultured dermal fibroblasts from each diabetic mouse. After 3rd passage of cell culture, each pellet of fibroblasts cultured autologously was transplanted into each wound of diabetic mouse themselves. Results: Wound healing of autologous diabetic dermal fibroblast-transplanted group showed better wound healing than that of allogeneic normal fibroblast-transplanted group. Induced diabetic dermal tissues showed similar growth factor activity with previously normal conditions. Immunohistochemical and real-time PCR about TGF-β and VEGF also showed better wound healing for the autologous wound group than that of the allogeneic normal fibroblasts cell transplanted group with statistical significance. Conclusion: Autologously cultured and transplanted wounds were seemed to show better healing in clinical, histochemical, immunohistochemical (TGF-beta, VEGF) and quantitative assays (TGF-beta, VEGF for the RT-PCR). Furthermore, diabetic fibroblasts can be expected to show similar effectiveness with normal conditions.
김미리(Kim, Mi-Ri),김태윤(Kim, Tae-Yun),박주아(Park, Joo-Ah),서예진(Seo, Ye-Jin),정은서(Jung, En-Seo),허윤서(Huh, Yoon-Seo),조운행(Cho, Un-Haing) 한국과학영재교육학회 2020 과학영재교육 Vol.12 No.2
To detect the water stress early before the appearance of morphological stress symptoms, the morphological changes, chlorophyll content, chlorophyll fluorescence and spectral reflectance of leaves were analyzed in Arabidopsis thaliana and Glycine max growing in water stress conditions. While the morphological changes including wilting, stem thinning and leaf rolling appeared clearly after 5 days exposure to drought, the increase of chlorophyll content and the decrease of chlorophyll fluorescence and spectral reflectance at 450-700 nm wavelength of leaves appeared after 3 days exposed to drought. The results obtained in this study indicate that drought enhances local chlorophyll content temporarily and reduces chlorophyll fluorescence and spectral reflectance early before the appearance of morphological changes. The light-related physiological parameters used in this experiment might tell us when the plants feel thirsty and could be used for us to decide when the water supply is not too late to minimize plant damage and to reduce yield loss. 외형적인 증상이 나타나기 전에 수분 스트레스의 조기 탐지가 가능한지를 알아보기 위해 가뭄에 노출하여 성장시킨 Arabidopsis thaliana와 Glycine max의 형태적 변화, 엽록소 농도, 엽록소 형광 및 반사율을 분석해 보았다. 시듦, 줄기 가늘어짐 및 잎 말림을 포함하는 형태적 변화가 가뭄 노출 5일 째부터 일어나는 반면 잎의 국부적인 엽록소 농도 증가와 엽록소 형광 및 500~600nm 영역에서의 반사율 저하는 가뭄 노출 2일째부터 나타났다. 본 실험 결과는 수분 스트레스가 형태적인 증상들이 나타나기 전에 일시적으로 엽록소 농도를 증가시키며 엽록소 형광과 반사율은 감소시킴을 보여준다. 본 실험에서 사용된 빛과 관련된 생리적 지표들은 식물이 물 부족을 언제 느끼며, 우리가 언제 물을 줘야 식물의 피해를 최소화하여 생산성 감소를 줄일 수 있는지를 알려줄 것이다.