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Mohamad Hamshou,Chenjing Shang,Jeroen De Zaeytijd,Els J.M. Van Damme,Guy Smagghe 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
Someribosome-inactivating proteins (RIPs) possess insecticidal activity. In this studywe investigated the activity of type 1 and type 2 RIPs fromapple (Malus domestica) against an economically important pest insect belonging to the order of Lepidoptera, the beet armyworm(Spodoptera exigua),with the use of tobacco plants overexpressing the apple RIPs. Insect bioassays using detached leaves fromtransgenic tobacco lines demonstrated that the ectopically expressed type 1 or type 2 RIPs from apple exerted a high entomotoxic activity.We scored up to 78% mortality during the larval stages of S. exigua.Moreover, a significant reduction in the larval weightwas observed in the surviving larvae fed on the transgenic lines at different time points as compared to wild type plants. In addition, apple RIPs also retarded the development of the larvae. These results provide further support for RIPs having a potential role in plant defense. It can be envisaged that these RIPs could be used in genetically engineered crops to enhance resistance against agronomically important insect pests.
Luo, Yongting,Xu, Xiaochao,Liu, Jiwei,Li, Jian,Sun, Yisheng,Liu, Zhen,Liu, Jinzhi,Damme, Els Van,Balzarini, Jan,Bao, Jinku Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3
A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.