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차월석,이동병,나재운 한국공업화학회 1999 응용화학 Vol.3 No.1
In this study, we examined about effectives of the release drug by deacetylation and molecular weight varieties of chitosan in DDS. The release experiments of chitosan tablets containing norfloxacin were operated in the phosphate buffer solutions of pH 7.4 and 37℃ and its results were as follows. In the linearly release time of drug, chitosan tablet of molecular weight 300,000 of 228 hr and molecular weight 220,000 of 145 hr showed effectives of deacetylation, their higher deacetylation rate were delayed longer than their lower deacetylation rate. In short, the formulation allows chitosan tablets to suppress the burst effect of the drug release mechanism, which led to the controlled release pattern and micelles.
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車月石,金千奎,金娟順 조선대학교 동력자원연구소 1985 動力資源硏究所誌 Vol.7 No.1
Crude protein and amino acids contents of three different rice brans from Milyang-23, Milyang-30, and Iri-342, new rice products in Korea were determined. Amino acids analysis was performed with an automatic amino acid analyzer, and crude protein content was determined by using a micro Kjeldahl apparatus 1. They include 13.58% to 15.65% of crude protein, especially the rice bran from Iri-342 has the richest. 2. Total amino acid contents of these brans are 10.94% for Milyang23, 10.68% for Milyang-30, and 12.21% for Iri-342 respectively. 3. Seven essential amino acids, lysine, isoleucine, leucine, plenylalanine, methionine threonine, valine are contained in the rice brans, especially total amounts of these ones is 14.1372% for I-Ri-342.
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Paramecium bursaria와 Chlorella의 共生體에 관한 硏究
차월석,김종수,김광욱 조선대학교 기초과학연구소 1988 自然科學硏究 Vol.11 No.1
In order to investigate the symbiosis of Paramecium bursaria and chlorella, changes of the chlorella amounts and the number of P.bursaria, and coating states inside the cytoplasm of the chlorella disappeared were studied with relation to the amounts of light irradiated. The results were as follows; (1) In continuous light, P.bursaria was separated and breeded in main, chlorella was breeded rapidly in the pure medium, on the other hand, in continuous darkness, Chlorella was breeded in half. (2) Chlorella in P.bursaria was essentially effected to the increase and growth of P.bursaria numbers and also was high in separation capacity. (3) P.bursaria was breeded by the separation according to the contacting process as well as the biseparation, that is, chlorella was not disappeared in P.bursaria, but it had a closed symbiotic relations with P.bursaria. (4) The symbiotic process was explained that chlorella moved into the medium by it self according to P.bursaria followed by over coming the enviromeatal conditions and making an appropriate condition.
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Studies on the Optimization of Culture Conditions and Antitumor Effects of Pleurotus ferulae
차월석 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.1
The objectives of this work are to determine the optimal culture conditions in liquid-state fermentation for production of P. ferulae, and to investigate the effects of P. ferulae extracts on viability of human cancer cell lines for screening the antitumor substances contained in P. ferulae. The optimal medium composition was glucose 5%, polypeptone 1%, yest extract 0.8%, K2HPO4 0.12%, and MgSO4 ·7H2O 0.12% (w/v). By using the optimized medium, mycelial and exopolysaccharide concentrations after 10 days with a 5-L jar fermenter were 13.2 g/L and 4.95 g/L,respectively. We proposed logistic model to describe the mycelial growth and Leudecking-Piret model for exo-polysaccharide formation in P. ferulae. As the results, developed model showed good agreement mycelial growth and exo-polysaccharide production. Ethanol extracts of P. ferulae fruiting body(PFF) showed strong cytotoxuicity against A549 cells at concentrations over 10 μg/mL. Also, PFF ethanol extracts induced synergistic effect on TRAIL-induced apoptosis in A549 cells, which were strong resistant to TRAIL. These results indicated that ethanol extracts of PFF were the most prominent antitumor agents for lung cancer cells (A549).
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아가리쿠스(Agaricus brasiliensis) 추출물의 항암 및 항산화 효과
차월석,정길록,김천환 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3
버섯에서 유효성분의 추출은 Dong 등(Dong et al., 2002)의 방법에서 최초 추출방법을 변형하여 실시하였는바, Dong 등 은 100℃에서 물중탕하여 추출하였으나 Guterrez 등(Guterrez et al., 2004)은 60℃에서 추출하는 것이 가장 바람직하다 고 보고한 바 있어, 본 연구에서는 Guterrez 등의 방법에 따라 60℃에서 물중탕하였으며, 다른 과정은 Dong 등의 방법에 준하여 유효성분을 추출하였다. 당 성분의 검정은 Lane 등의 방법에 따라 phenol-sulfuric acid 법(Chaplin and Kennedy, 1994)을 이용하였다. 항암 기능성물질은 추출, 분리 및 정제과정에서 수집된 각 분획을 취하여 syringe filter (0.22μm)를 통과시킨 후 항암성 물질 검색시료로 사용하였다. 항산화 효과 test는 아가리쿠스버섯 자실체 50g에 증류수 500ml를 가하 고 100℃에서 1시간동안 열수 처리하여 얻은 물추출액과 메탄올 500ml를 가하여 상온에서 36시간 동안 방치한 후 얻어 진 메탄올 추출액을 시료로 사용하였다. 항암 기능성물질은 배양 암세포에 첨가하여 나타나는 세포독성을 사립체 탈수소 효소에 의해서 MTT가formazan을 형성하는 반응원리에 의한 MTT법으로 측정하였다. 아가리쿠스버섯에 함유된 기능성 물질의 항산화 효과를 보기 위하여 흔히 사용되는 항산화 시험법 중의 하나인 Lipid peroxdation(지질과산화) Test인 TBA Test를 시행하였다. 폐암세포(A549)에 대한 2종의 버섯 추출물 분리, 정제 분획을 투여하여 세포독성을 관찰한 결 과 ethanol의 DEAE-cellulose 크로마토그래피 물추출분획(F4)의 세포독성 효과가 가장 큰 것으로 나타났다. 또한 아가 리쿠스의 메탄올 추출액은 현재 가장 널리 사용하는 항산화제인 BHA와 비슷한 수준의 항산화 효과를 보여주었으며 메탄 올 추츨액이 물 추출액보다 항산화 효과가 더 우수하였다.
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차월석,이동병,강시형,오동규 한국생명과학회 2003 생명과학회지 Vol.13 No.4
This Study was carried out to investigate the optimal mycelial growth of Pholiota nameko. The optimal medium for the mycelial growth was ME medium. The optimal temperature and pH were $25^{\circ}C$$\pm$1 and 5.5, respectively. The modified optimal medium compositions were glucose 3% (w/v), malt extract 0.25% (w/v), yeast extract 0.25% (w/v), $KH_2PO_4$ 0.046% (w/v), $K_2HPO_4$ 0.1% (w/v), $MgSO_4$ㆍ$7H_2O$ 0.05% (w/v). From the result of experiments on the optimal temperature, pH and nutritional requirements, the mycelial growth of modified optimal medium was higher than that of ME medium. 맛버섯( Pholiota nameko KACC50453)의 균사체 배양 특성에 관한 연구 결과 맛버섯 균사체의 배양에 적합한 기본 배지는 ME였으며, 최적 배양 온도는 $25^{\circ}C$, 최적 초기 pH는 5.5였다. 맛버섯 균사체 생장을 위한 최적 배지 조성은 glucose 3%(w/v),malt extrac 0.25%(w/v), yeast extract 0.25%(w/v),$KH_2PO_4$ 0.046%(w/v),$K_2HPO_4$ 0.1%(w/v),$MgSO_4$ $.$$7H_2O$0.05% (w/v)로 나타났다.기본배지(ME)와 최적배지로 액체 배양한 결과 최적 배지에서 균사생장이 양호하였다.
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