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Ahn, Dalrae,Lee, Eun Byeol,Kim, Ban Ji,Lee, So Yeon,Lee, Tae Gwan,Ahn, Min-Sil,Lim, Hye Won,Cha, Dong Seok,Jeon, Hoon,Kim, Dae Keun The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.6
Quercetin-3-O-${\alpha}$-$\small{L}$-rhamnopyranosyl($1{\rightarrow}2$)-O-${\alpha}$-$\small{L}$-rhamnopyranoside (QDR) was isolated from the remaining underground parts of Curcuma longa after harvesting the medicinal parts, and the antioxidant activities in vitro and lifespan-extending effect of QDR were elucidated using the Caenorhabditis elegans model system. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of QDR showed similar potent activities in comparison with vitamin C. QDR also showed strong superoxide quenching activities as measured by the riboflavin- and xanthine-originated superoxide quenching activities. QDR demonstrated potent lifespan extension of worms under normal culture condition. Subsequently, the protective effect of QDR on the stress conditions such as thermal and oxidative stresses was determined. In the case of heat stress, QDR-treated worms exhibited enhanced survival rate, as compared to control worms. In addition, QDR-fed worms lived longer than control worms under oxidative stress induced by paraquat. To verify the possible mechanism of QDR-mediated increased lifespan and stress resistance of worms, we investigated whether QDR might alter superoxide dismutase (SOD) activity and intracellular reactive oxygen species (ROS) levels. Our results showed that QDR was able to elevate SOD activity of worms and reduce intracellular ROS accumulation in a dose-dependent manner.
Superoxide Radical Scavengers from the Whole Plant of Veronica peregrina
Dalrae Ahn,Sa Im Lee,Jae Heon Yang,Chong Hyeon Cho,Yong-Hun Hwang,Jong-Han Park,김대근 한국생약학회 2011 Natural Product Sciences Vol.17 No.2
In the course of screening for antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl- 2-picrylhydrazyl (DPPH), a total extract from the whole plant of Veronica peregrina (Scrophulariaceae) was found to show potent antioxidant activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of six phenolic compounds including chrysoeriol (1), diosmetin (2), 4-hydroxybenzoic acid (3), apigenin (4), caffeic acid methylester (5) and protocatechuic acid (6). Their structures were elucidated by spectroscopic studies. Compounds 1-5 were isolated for the first time from this plant. Compounds 5 and 6 showed significant antioxidative effects in DPPH free radical scavenging and superoxide quenching activity assays.
Radical Scavenging Constituents of Cyperus flaccidus
Dalrae Ahn,Hyunjin Lee,Ming Ming Xing,Chao Tao,Eun Byeol Lee,Tae Gwan Lee,신태용,은재순,전훈,임종필,김대근 한국생약학회 2012 Natural Product Sciences Vol.18 No.2
In the course of screening for antioxidant compounds by measuring the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging effect and superoxide quenching activity, methanol extract of Cyperus flaccidus (Cyperaceae) was found to show potent antioxidant activities. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of ten compounds, kaempferol-3,7-O-α-L-dirhamnopyranoside (1),caffeic acid (2), quercetin-3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (3), kaempferol-3-O-α-Lrhamnopyranoside (4), quercetin-3-O-α-L-rhamnopyranoside (5), luteoiln-7-O-β-D-glucopyranoside (6), kaempferol-3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (7), luteoiln (8), quercetin (9) and quercetin-3-O-β-Dglucuronide (10). Their structures were elucidated by spectroscopic studies. Compounds 1 - 10 were isolated for the first time from this plant. Among them, compounds 2, 8 and 9 showed the significant radical scavenging effects on DPPH, and the potent xanthine-originated superoxide quenching activities.
Iridoid Glycosides from the Aerial Parts of Galium spurium L.
Dalrae Ahn,김대근 한국생약학회 2012 Natural Product Sciences Vol.18 No.3
Nine iridoid glycoside derivatives were elucidated from the methanolic extract of the aerial parts of Galium spurium (Rubiaceae) through repeated column chromatography. Their chemical structures were characterized as 10-O-trans-p-coumaroylscandoside (1a), 10-O-cis-p-coumaroylscandoside (1b), 10-O-trans-p-coumaroyl-10-O-deacetyldaphylloside (2), 10-O-cis-p-coumaroyl-10-O-deacetyldaphylloside (3), asperulic acid methylester (4), asperuloside (5), asperulosidic acid (6), scandoside (7), and deacetyl asperulosidic acid (8) by spectroscopic analysis. This is the first report of the characterization of compounds 1a, 1b, 2, 3 and 7 from this plant.
Genistein from Vigna angularis Extends Lifespan in Caenorhabditis elegans
( Eun Byeol Lee ),( Dalrae Ahn ),( Ban Ji Kim ),( So Yeon Lee ),( Hyun Won Seo ),( Youn Soo Cha ),( Hoon Jeon ),( Jae Soon Eun ),( Dong Seok Cha ),( Dae Keun Kim ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.1
The seed of Vigna angularis has long been cultivated as a food or a folk medicine in East Asia. Genistein (4′,5,7-trihydroxyisoflavone), a dietary phytoestrogen present in this plant, has been known to possess various biological properties. In this study, we investigated the possible lifespan-extending effects of genistein using Caenorhabditis elegans model system. We found that the lifespan of nematode was significantly prolonged in the presence of genistein under normal culture condition. In addition, genistein elevated the survival rate of nematode against stressful environment including heat and oxidative conditions. Further studies demonstrated that genistein-mediated increased stress tolerance of nematode could be attributed to enhanced expressions of stress resistance proteins such as superoxide dismutase (SOD-3) and heat shock protein (HSP-16.2). Moreover, we failed to find genistein-induced significant change in aging-related factors including reproduction, food intake, and growth, indicating genistein exerts longevity activity independent of affecting these factors. Genistein treatment also led to an up-regulation of locomotory ability of aged nematode, suggesting genistein affects healthspan as well as lifespan of nematode. Our results represent that genistein has beneficial effects on the lifespan of C. elegans under both of normal and stress condition via elevating expressions of stress resistance proteins.