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- 저자 : ( Ali Ozant ) (검색결과 1 건)
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Efficacy of Platelet-Rich Fibrin on Bile Duct Anastomosis in a Rat
( Necdet Ozcay ),( Ali Ozant ),( Hanife Ozkayalar ),( Hasan Besim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Promoting the healing process and increasing the regen erative capacity of the biliary system might play a crucial role in preventing biliary complications following hepatopancreaticobi liary operations and liver transplantation. Aim of the study is to evaluate the effect of platelet-rich-fibrin (PRF) concentrate on the bile duct anastomosis healing process in rats. Methods: Thirty male Sprague Dawley (SD) rats were used for the study. The animals were allocated into three groups: Group I Control Group (n=10): Anastomosis to the common bile duct (CBD) with a stent. Group II PRF Group (n=10): Anastomosis to the CBD with a stent and covered with PRF. Group III Sham Group (n=10): Preparation of the common bile duct, no anas tomosis. The animals were followed up for 1 month, then sac rificed. Study parameters were adhesions around the anasto mosis, bridging the bile duct tissue thickness over the stent and histopathologic examination of the bridging bile duct tissue. Results: CBD anastomosis using a stent caused severe adhesion around the anastomosis, bridging bile duct tissues were weak and histopathologically, healing was incomplete in most of the control animals. However, PRF application significantly reduced the adhesions, increased the quality of the bridging bile duct tissues and caused complete healing histologically. Conclusions: PRF is a safe, autologous, easily prepared mem brane. The present study findings shows that PRF prevents local complications, and increases the healing capacity of the bile duct after CBD anastomosis. Therefore, it might be a new treatment option for preventing bile duct complications in liver transplantation patients.
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