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Gamze ER,Alexander JAHN,Shah Abid ALI,Man-Gi CHO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
Biofilm-based Photobioreactors (PBRs) use microalgal biomass immobilized on a substrate with reduced water content and consumption when compared to conventional suspended cultivation. CO2 and carbonate are used by microalgae as inorganic carbon source and their consumption is an indicator for metabolic activity and biomass accumulation. Here CO2 uptake, holdup and release in and from the culture were measured and correlated with gravimetrically determined biofilm mass. For this, a wicking based flat panel biofilm PBR including CO2/O2 gas control was developed, and the CO2 uptake and holdup determined by CO2 NIR off-gas analysis. The cyanobacteria Chlamydomonas asymmetrica were cultivated on hydrophilic modified expanded UHMWPE plates over circulating AF6 medium at 30°C and fluorescent light illumination about 710.01 µW/cm<sup>2</sup>. CO2 holdup in the system was subtracted from values determined during biofilm cultivation at stationary and growth conditions and correlated with measured biomass. From the gas holdup a minimum feasible cultivation size, dependent on the NIR-CO2 sensor sensitivity, for the use of the developed method was estimated. Results suggest the potential use of gas holdup measurement for biofilm growth assessment in medium and large-scale closed cultivations.
Moment-curvature hysteresis model of angle steel frame confined concrete columns
Chong Rong,Wenkai Tian,Qingxuan Shi,Bin Wang,Abid Ali Shah 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.1
The angle steel frame confined concrete columns (ASFCs) are an emerging form of hybrid columns, which comprise an inner angle steel frame and a concrete column. The inner angle steel frame can provide axial bearing capacity and well confining effect for composite columns. This paper presents the experimental and theoretical studies on the seismic behaviour of ASFCs. The experimental study of the 6 test specimens is presented, based on the previous study of the authors. The theoretical study includes two parts. One part establishes the section analysis model, and it uses to analyze section axial force-momentcurvature. Another part establishes the section moment-curvature hysteresis model. The test and analysis results show that the axial compression ratio and the assembling of steel slabs influence the local buckling of the angle steel. The three factors (axial compression ratio, content of angle steel and confining effect) have important effects on the seismic behaviour of ASFCs. And the theoretical model can provide reasonably accurate predictions and apply in section analysis of ASFCs.
Estimation of Antibacterial Properties of Chlorophyta, Rhodophyta and Haptophyta Microalgae Spe
( Khawaja Muhammad Imran Bashir ),( Jae-hyeon Lee ),( Maike Julia Petermann ),( Abid Ali Shah ),( Su-jin Jeong ),( Moo-sang Kim ),( Nam-gyu Park ),( Man-gi Cho ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 한국미생물·생명공학회지 Vol.46 No.3
In this exploratory study, eight types of microalgae from different phyla (Chlamydomonas reinhardtii, Chlorella species, Haematococcus pluvialis, Porphyridium purpureum, Porphyridium cruentum, Isochrysis species, Isochrysis galbana, and Pavlova lutheri) were tested for their antibacterial activities against eight target pathogenic bacterial strains. The agar well diffusion method and broth micro dilution assay were conducted to estimate the antibacterial activity. Microalgae cell-free supernatants, exopolysaccharides (EPS), water, and organic solvent extracts were used for inhibition analysis. EPS extracted from P. lutheri showed activity against Bacillus subtilis and Pseudomonas aeruginosa. Inhibition zone diameters of 14- 20 mm were recorded on agar plates, while the minimum inhibitory concentrations in the broth micro dilution assay were 0.39-25 mg ml<sup>-1</sup>. During this study, haptophyte microalgae, Isochrysis species, and P. lutheri extracts showed the highest activity against most of the tested pathogenic bacterial strains, while most of the extracts were active against the important foodborne pathogen P. aeruginosa. This study showed promising results regarding important microalgae phyla, which will further aid research related to extracts and exploitation of bioactive metabolic compounds in the food and pharmaceutical industries.
Estimation of Antibacterial Properties of Chlorophyta, Rhodophyta and Haptophyta Microalgae Species
Imran Bashir, Khawaja Muhammad,Lee, Jae-Hyeon,Petermann, Maike Julia,Shah, Abid Ali,Jeong, Su-Jin,Kim, Moo-Sang,Park, Nam-Gyu,Cho, Man-Gi The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.3
In this exploratory study, eight types of microalgae from different phyla (Chlamydomonas reinhardtii, Chlorella species, Haematococcus pluvialis, Porphyridium purpureum, Porphyridium cruentum, Isochrysis species, Isochrysis galbana, and Pavlova lutheri) were tested for their antibacterial activities against eight target pathogenic bacterial strains. The agar well diffusion method and broth micro dilution assay were conducted to estimate the antibacterial activity. Microalgae cell-free supernatants, exopolysaccharides (EPS), water, and organic solvent extracts were used for inhibition analysis. EPS extracted from P. lutheri showed activity against Bacillus subtilis and Pseudomonas aeruginosa. Inhibition zone diameters of 14-20 mm were recorded on agar plates, while the minimum inhibitory concentrations in the broth micro dilution assay were $0.39-25mg\;ml^{-1}$. During this study, haptophyte microalgae, Isochrysis species, and P. lutheri extracts showed the highest activity against most of the tested pathogenic bacterial strains, while most of the extracts were active against the important foodborne pathogen P. aeruginosa. This study showed promising results regarding important microalgae phyla, which will further aid research related to extracts and exploitation of bioactive metabolic compounds in the food and pharmaceutical industries.
Abdul Haleem,Sidra Bibi Syaal,Muhammad Ajmal,Jaweria Ambreen,Sajid Rauf,Nasir Ali,Saz Muhammad,Afzal Shah,Muhammad Abid Zia,Muhammad Siddiq 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.4
Here in, we demonstrate facile fabrication of silver and palladium nanoparticles in dual responsive poly(Nisopropylacrylamide- co-2-Acrylamido-2-methylpropane sulfonic acid) microgel with temperature- and pH-dependent catalytic potential. Palladium-based catalyst showed better catalytic efficiency as compared to silver-based catalyst for degradation of Rhodamine-B and P-Nitrophenol in aqueous medium under the same set of reaction conditions. The responsive nature of the microgel was found to be useful to tune the catalytic activity of the as-prepared catalysts, and reduction rate was enhanced with the pH and temperature elevation of the reaction medium; however, the increasing trend was slowed in the volume phase transition region of the microgel. Under a specific set of reaction conditions, the reduction of Rhodamine-B was as fast as 0.968 and 0.571 min1 when catalyzed with palladium and silver based catalysts, respectively. The hydrodynamic radius of the particles of microgel support was found to be in the range of 65- 180 nm when pH and temperature of the medium were varied in the range of 2-12 and 25-45, respectively. The estimated diameter of silver and palladium nanoparticles fabricated in the microgel support under the same set of reaction conditions was 9-15 and 7-11 nm, respectively.