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Yongqin Pan,Genlong Jiao,Jingge Yang,Rui Guo,Jinyi Li,Cunchuan Wang 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.3
Recurrent laryngeal nerve (RLN) injury can result in unilateral or bilateral vocal cords paralysis, thereby causing a series of complications, such as hoarseness and dyspnea. However, the repair of RLN remains a great challenge in current medicine. This study aimed to develop human umbilical mesenchymal stem cells (HuMSCs) and nerve growth factor (NGF)-loaded heparinized collagen scaffolds (HuMSCs/NGF HC-scaffolds) and evaluate their potential in the repair of RLN injury. HuMSCs/NGF HC-scaffolds were prepared through incorporating HuMSCs and NGF into heparinized collagen scaffolds that were prefabricated by freeze-drying in a template. The resulting scaffolds were characterized by FTIR, SEM, porosity, degradation in vitro, NGF release in vitro and bioactivity. A rabbit RLN injury model was constructed to appraise the performance of HuMSCs/NGF HC-scaffolds for nerve injury repair. Electrophysiology, histomorphology and diagnostic proteins expression for treated nerves were checked after application of various scaffolds. The results showed that the composite scaffolds with HuMSCs and NGF were rather helpful for the repair of broken RLN. The RLN treated with HuMSCs/NGF HC-scaffolds for 8 weeks produced a relatively normal electromyogram, and the levels of calciumbinding protein S100, neurofilament and AchE pertinent to nerve were found to be close to the normal ones but higher than those resulted from other scaffolds. Taken together, HuMSCs/NGF HC-scaffolds exhibited a high score on the nerve injury repair and may be valuable for the remedy of RLN injury.
Yongqin Zhou,Bo Hu,Hang Wang,Ningzhi Jin,Meilan Zhou 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.2
In this paper, a flux linkage model based on four magnetization curves fitting is proposed for three-phase 12/8 switched reluctance motor (SRM), with the analysis of the basic principle of flux detection method and function analysis method. In the model, the single value function mapping relationship between position angle and flux is established, which can achieve a direct estimation of rotor position. The realization scheme of SRM indirect position detection system is presented. It is proved by simulation and experiment that the proposed scheme is suitable for rotor position detection of SRM, and has high accuracy of position estimation.
Zhou Yongqin,Huang Ce,Chen Junjie,Shi Guijun,Zhou Meilan 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.6
Due to synchronous rectification based on Phase-shift full bridge converter is difficult to work in a state of soft switch, to solve this problem, this paper sets out an control approach of synchronous rectification based on the ZVZCS phase-shifted full-bridge, and the drive signal of synchronous rectification side uses leading-leg drive waveform after a certain time delay and applies the resonant principle in the switching process,not only keeping full bridge ZVZCS soft switching but also realizing MOSFET of the synchronous rectification ZVS. The simulation of saber and experimental research show that the converter has a good performance.
Zhou, Yongqin,Hu, Bo,Wang, Hang,Jin, Ningzhi,Zhou, Meilan The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.2
In this paper, a flux linkage model based on four magnetization curves fitting is proposed for three-phase 12/8 switched reluctance motor (SRM), with the analysis of the basic principle of flux detection method and function analysis method. In the model, the single value function mapping relationship between position angle and flux is established, which can achieve a direct estimation of rotor position. The realization scheme of SRM indirect position detection system is presented. It is proved by simulation and experiment that the proposed scheme is suitable for rotor position detection of SRM, and has high accuracy of position estimation.
Xianglin Hou,Yongqin Qi,Xingang Qiao,Guofu Wang,Zhangfeng Qin,Jianguo Wang 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.2
acid catalysts are active for both esterification and transesterification, but the reaction is very slowdue to mass-transfer limitations betwen methanol and oil phase. Because oil, FFA and Lewis acid catalysts are allsoluble in the subcritical methanol phase, the esterification and transesterification will be enhanced when they are carriedout under subcritical conditions. In this work, the esterification and transesterification of high FFA oil to biodiesel viaLewis acid catalysts such as Pb(OOCH3)2, Cd(OOCCH3)2 and Zn(OOCCH3)2 were carried out in the subcritical meth-anol phase (2 MPa, 180oC, reaction time 30 min). The results show that the esterification conversion reaches 79.8-96.4% with Palmitic acid as feedstock, and the transesterification conversion reaches 56.8-73.4% with soybean oil asfeedstock. With the mixture of Soybean oil and Palmitic acid (FA content of 20.3 wt% ) as feedstock, the content offatty acid methyl esters (FAME) in products reaches 67.3-83.4% .
Intensifying sulforaphane formation in broccoli sprouts by using other cruciferous sprouts additions
Hao Liang,Yongqin Wei,Ruimin Li,Li Cheng,Qipeng Yuan,Fuping Zheng 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
Sulforaphane is a significant chemopreventive compound which is the predominant glucosinolate in broccoli sprouts. However, the existence of the epithiospecifier protein could direct the hydrolysis of glucosinolates toward sulforaphane nitrile formation instead of sulforaphane. Therefore, the study aimed on improving the yielding of sulforaphane in broccoli sprouts with a new method of the united hydrolysis of cruciferous sprouts. According to the results, the addition of radish, rocket and rape sprouts to broccoli sprouts could promote the hydrolysis of the glucoraphanin to anticancer effective sulforaphane to 2.03, 2.32 and 1.95-fold, respectively, compared to single broccoli sprouts. Meanwhile, the formation of non-bioactive sulforaphane nitrile in these three groups decreased greatly. However, the addition of mustard sprouts had no positive effect. These observations could make a contribution to the potential chemoprotective effects of broccoli sprouts.