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ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light
Kim, Woe-Yeon,Fujiwara, Sumire,Suh, Sung-Suk,Kim, Jeongsik,Kim, Yumi,Han, Linqu,David, Karine,Putterill, Joanna,Nam, Hong Gil,Somers, David E. Nature Publishing Group 2007 Nature Vol.449 No.7160
The circadian clock is essential for coordinating the proper phasing of many important cellular processes. Robust cycling of key clock elements is required to maintain strong circadian oscillations of these clock-controlled outputs. Rhythmic expression of the Arabidopsis thaliana F-box protein ZEITLUPE (ZTL) is necessary to sustain a normal circadian period by controlling the proteasome-dependent degradation of a central clock protein, TIMING OF CAB EXPRESSION 1 (TOC1). ZTL messenger RNA is constitutively expressed, but ZTL protein levels oscillate with a threefold change in amplitude through an unknown mechanism. Here we show that GIGANTEA (GI) is essential to establish and sustain oscillations of ZTL by a direct protein–protein interaction. GI, a large plant-specific protein with a previously undefined molecular role, stabilizes ZTL in vivo. Furthermore, the ZTL–GI interaction is strongly and specifically enhanced by blue light, through the amino-terminal flavin-binding LIGHT, OXYGEN OR VOLTAGE (LOV) domain of ZTL. Mutations within this domain greatly diminish ZTL–GI interactions, leading to strongly reduced ZTL levels. Notably, a C82A mutation in the LOV domain, implicated in the flavin-dependent photochemistry, eliminates blue-light-enhanced binding of GI to ZTL. These data establish ZTL as a blue-light photoreceptor, which facilitates its own stability through a blue-light-enhanced GI interaction. Because the regulation of GI transcription is clock-controlled, consequent GI protein cycling confers a post-translational rhythm on ZTL protein. This mechanism of establishing and sustaining robust oscillations of ZTL results in the high-amplitude TOC1 rhythms necessary for proper clock function.
Kim, Woe-Yeon,Cheong, Na-Eun,Lee, Hoon-Sil,Lee, Dong-Chul,Je, Dae-Teop,Bahk, Jeong-Dong,Lee, Sang-Yeol Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
A cDNA clone encoding an α subunit of guanine nucleotide-binding protein (G protein), SGA1, was isolated from a soybean cDNA library and its nucleotide sequence was analyzed. The cDNA encodes a distinct protein which shows considerable amino acid sequence identity to the plant G protein α subunits of Arabidopsis thaliana (82.1%) and tomato(81.3%). However, it shares low sequence homology between 30% and 40% with the yeast and mammalian G protein α subunits. SGA1, a 1,624bp long, encodes a 44.2kD protein which has several conservative peptide domains appeared in all eucaryotic G protein α subunits. Even though the expression level of SGA1 gene is relatively low, it is predominantly transcribed in the actively proliferating elongation region of soybean seedlings. Southern blot analysis using the cDNA insert of SGA1 as a probe suggested that the α subunits of soybean G protein consisted a multigene family in soybean genome.
LNG 선의 1차 방벽 스테인리스 스틸 멤브레인 주름부의 내압 성능에 관한 연구
박성연(Seong Yeon Park),김외태(Woe Tae Kim),김성수(Seong Su Kim) 대한기계학회 2019 대한기계학회 춘추학술대회 Vol.2019 No.11
Corrugated stainless-steel membranes have been used to reduce thermal stress at cryogenic temperature as the primary barrier for LNG containment systems to contain the cargo of LNG. As the capacity of LNG cargo increases, failure of the corrugated stainless-steel membranes in the form of buckling and collapsing can be occurred due to increased sloshing pressure of the LNG cargo. The failure of the primary barrier results in LNG leakage, which lead to tremendous economic loss. Therefore, it is necessary to analyze the pressure-resisting capability of the corrugated structure of the primary membrane accurately. In this work, metal forming analysis by using a finite element method was performed to calculate residual stress generated during the fabrication of the corrugated structure. Thereafter, the pressure-resisting capability was evaluated by considering the result of forming analysis. To verify the numerical results, a testing device was developed to measure the pressure-resisting capability of the corrugated structure and the experimental results are compared with the numerical results.
A role for GIGANTEA : Keeping the balance between flowering and salinity stress tolerance
Park, Hee Jin,Kim, Woe-Yeon,Yun, Dae-Jin Landes Bioscience 2013 Plant signaling & behavior Vol.8 No.7
<P>The initiation of flowering in <I>Arabidopsis</I> is retarded or abolished by environmental stresses. Focusing on salt stress, we provide a molecular explanation for this well-known fact. A protein complex consisting of GI, a clock component important for flowering and SOS2, a kinase activating the [Na<SUP>+</SUP>] antiporter SOS1, exists under no stress conditions. GI prevents SOS2 from activating SOS1. In the presence of NaCl, the SOS2/GI complex disintegrates and GI is degraded. SO2, together with the Ca<SUP>2+</SUP>-activated sensor of sodium ions, SOS3, activates SOS1. In <I>gi</I> mutants, SOS1 is constitutively activated and <I>gi</I> plants are more highly salt tolerant than wild type <I>Arabidopsis.</I> The model shows GI as a transitory regulator of SOS pathway activity whose presence or amount connects flowering to environmental conditions.</P>