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Influence of Tyrosol on Cell Growth Inhibition of KB Human Oral Cancer Cells
Ue-Kyung Lee,Su-Gwan Kim,Dae-San Go,Sun-Kyoung Yu,Chun Sung Kim,Jeongsun Kim,Do Kyung Kim 대한구강생물학회 2016 International Journal of Oral Biology Vol.41 No.4
Tyrosol, a phenylethanoid and a derivative of phenethyl alcohol, possesses various biological properties, such as anti-oxidative and cardioprotective activity. Olive oil is the principal source of tyrosol in the human diet. However, so far the anti-cancer activity of tyrosol has not yet been well defined. This study therefore undertakes to examine the cytotoxic activity and the mechanism of cell death exhibited by tyrosol in KB human oral cancer cells. Treatment of KB cells with tyrosol induced the cell growth inhibition in a concentration- and a time-dependent manner. Furthermore, the treatment of tyrosol induced nuclear condensation and fragmentation of KB cells. Tyrosol also promoted proteolytic cleavage of procaspase-3, -7, -8 and –9, increasing the amounts of cleaved caspase-3, -7, -8 and –9. In addition, tyrosol increased the levels of cleaved PARP in KB cells. These results suggest that tyrosol induces the suppression of cell growth and cell apoptosis in KB human oral cancer cells, and is therefore a potential candidate for anti-cancer drug discovery.
김의경(Ue Kyung Kim) 대한공업교육학회 1988 대한공업교육학회지 Vol.13 No.1
The purpose of this study is to investigate learning effects by computer assisted instruction in industrial education and vocational training. This study is a experimental reserch through the two groups which has the significant condition, the one was control group, and the other was experimental group. The major findings of the study were as follows: 1. If we take advantage of computer in industrial education and vocational training, we can reduce expenses for equipments and facilities. 2. Because teacher and instructor can simply present intricate structure and operation method to student in CAI, teacher and instructor can reduce learning hours and skill training is easy. 3. Because we can use simulation in CAI, actively we can cope with change of education and training equipments, and practice with dangerous and expensive equipments, too. 4. If we make full use of CAI, we can do a theory and practice learning at the same time.
Parkin induces apoptotic cell death in TNF-α-treated cervical cancer cells
( Kyung Hong Lee ),( Min Ho Lee ),( Yeo Wool Kang ),( Ki Jong Rhee ),( Tae Ue Kim ),( Yoon Suk Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.9
Many malignant tumors become resistant to tumor necrosis factor-alpha (TNF-α)-induced cell death during carcinogenesis. In the present study, we examined whether parkin acts as a tumor suppressor in HeLa cells, a human cervical cancer cell line resistant to TNF-α-induced cell death. TNF-α-treatment alone did not affect HeLa cell viability. However, expression of parkin restored TNF-α-induced apoptosis in HeLa cells. Increased cell death was due to the activation of the apoptotic pathway. Expression of parkin in TNF-α-treated HeLa cells stimulated cleavage of the pro-apoptotic proteins caspase-8, -9, -3, -7 and poly ADP ribose polymerase (PARP). In addition, parkin expression resulted in decreased expression of the caspase inhibitory protein, survivin. These results suggest that parkin acts as a tumor suppressor in human cervical cancer cells by modulating survivin expression and caspase activity. We propose that this pathway is a novel molecular mechanism by which parkin functions as a tumor suppressor. [BMB Reports 2012; 45(9): 526-531]
A Biochemical Study on the Cytolytic Substance of Acanthamoeba culbertsoni
Im,Kyung-Il,Cho,Young-Dong,Kim,Tae-Ue INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1989 YONSEI REPORTS ON TROPICAL MEDICINE Vol.20 No.1
Cytolytic substance of pathogenic free living amoeba, Acanthamoeba culbertsoni, has been claimed to be closely related to meningoencephalitis. To characterize and reveal properties and action mechanism of cytolytic substance, the following methods were used. Identification of A. Culbertsoni causing death was made by detecting the amoeba from the brain of the dead mouth which was sacrified by infection of the amoeba. For cytolytic assay of cytolytic substance, Chinese hamster ovary cell line was used. The isolation and purification of cytolytic substance involves ultrasonication, ultracentrifugation, ammonium sulfate fraction, and column chromatography such as CM-Trisacryl M, DEAE-Trisacryl M, Ultrogel AcA 54, hydroxyapatite and Con, A sepharose 4B. Purification fold and yield was 40 times and 11% and purity was determined by observing one protein band on gel electrophoresis. Molecular weight and subunit of the cytolytic substance were turned out to be 33,000 dalton and monomer by gel filteration and SDS PAGE.
이의진(Ue Jin Lee),김명기(Myung-ki Kim),이재관(Jae Kwan Lee),이상호(Sang Ho Lee),강경태(Kyung-Tae Kang) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.11
An inkjet printing among these printing techniques has been extensively demonstrated with attractive advantages regarding high registration accuracy of direct writing in targeted active area without unnecessary waste of functional materials. The 3% of highefficiency in inkjet printed organic bulk heterojunction solar cell using P3HT:PCBM composite ink in mixed solution of 68% of ortho-dichlorobenzene (oDCB) and 32% of mesitylene has been recently reported.³ However, although a high boiling ink formulation with oDCB or its mixed solvent was irresistibly applied to prevent vaporization of the solvent at the nozzle orifice, its use seems to be hard to control the morphology of BHJ film on PEDOT:PSS underlayer compared to that spin-cast. This paper presents that direct writing of P3HT:PCBM composite in BHJ solar cell can be efficiently addressed by inkjet printing technology using conventional chlorobenzene ink solution.
Study on Autogenous Behaviour of Aedes togoi, the Vector of Brugian Filariasis in Korea
Im, Kyung-lI,Shin, Ho-Joon,Kim, Tae-Ue,Ree, Han-lI INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1988 YONSEI REPORTS ON TROPICAL MEDICINE Vol.19 No.1
마레이 사상충증 매개종인 토고숲모기(Aedes togoi)의 비흡혈산란 습성에 관한 연구를 수행하기 위하여 1988년 예비조사를 실시하여 다음과 같은 결과를 얻었다. 1. 해안가 서식처에서 채집한 토고숲모기 제4령기 유충과 번데기를 실험실에서 우화시켜 얻은 자충을 10% 설탕물로 사육하면서 산란 유뮤를 관찰한 바 비흡혈산란률은 일광(부산근처) 41.6%, 흑산도 3.1%, 제주도 24.8% 및 완도 3.1%로 지역에 따라 심한 차이를 보였다. 2. 우화 10여일후 난소를 해부하여 뇨포의 발육상태를 관찰한 바 흡혈산란 개체(anautogeny strain)들은 대부분 Christophers 제IIa기에서 중지되어 있고 극히 소수의 개체에서 뇨포의 일부가 Christophers 제IIb기까지 발육이 진행되어 있었다. 반면에 비흡혈산란 개체 (autogeny strain)의 경우 일부 뇨포가 Christophers 제IV~V기까지 발육한 것을 관찰할 수 있었다. 3. 토고숲모기의 나소내 총 뇨포 수는 최저 102개, 최고 207개였고 평균 160.8개였다. 무흡혈로 산란한 알수는 제주도 22.0±11.9, 흑산도 28.1±15.0, 일광 46.5±26.8로 총 뇨포중 평균 20.1%가 완전하게 발육하였다. 반면에 흡혈로 산란한 알수는 107.4 ±15.5로 총 뇨포의 66.8%에 해당한다. 4. SDS polyacrylamide gel을 사용한 전기영동법으로 단백질 양상을 비교한 바 뇨포의 발육 상태가 다른 세 실험군, 즉 Christophers I-II~IIa기, III기 및, IV-V기간에 차이를 관찰할 수 없었다. The prelominary study on the autogenous behaviour of Aedes togoi, the vector species of Brugia malayi was carried out in 1988, and the results are summarized as follows: (1) The autogeny rate among the matural populations of Aedes togoi was 24.8% in Chejudo in May, 3.1% in Daehugsando in May-June, 41.6% at llkwang(near Pusan) in June and 3.1% in Wando in September. (2) Ovary dissection of the females after two weeks of emergence showed that the development of the follicles was stopped at Christophers stage lla(in a few females, small proportion of the follicles were stage llb) in the anautogenous strain, and 20.1% of the total follicles was developed to the stages IV-V in the autogeny strain. (3) The total number of the follicles of Aedes togoi was 164.0±25.0 and number of eggs laying in the autogenous condition was 22.0±11.9 in Chejudo strain, 28.1±15.0 on Daehugsando strain, 46.5±26.8 in llkwang strain and 8.5 (n=2) in Wando strain. Number of eggs laid after a blood meal was 107.4±15.5. (4) The perliminary result of the SDSpolyacrylamide gel electrophoresis indicated that there was no difference in the protein patterns between the autogeny and the anautogeny strains.