Synergistic Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma in Streptozotocin-Induced Diabetic Rats

( Zhen Zhen Lian ),( Xiao Jing Yin ),( Hua Li ),( Li Li Jia ),( Xiu Zhen He ),( Yong Bo Yan ),( Nai Hua Liu ),( Ka Yiu Wan ),( Xiao Kun Li ),( Shao Qiang Lin ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.1

Background: Diabetic wounds are a major clinical challenge, because minor skin wounds can lead to chronic, unhealed ulcers and ultimately result in infection, gangrene, or even amputation. Studies on bone marrow derived mesenchymal stem cells (BMSCs) and a series of growth factors have revealed their many benefits for wound healing and regeneration. Platelet-rich plasma (PRP) may improve the environment for BMSC development and differentiation. However, whether combined use of BMSCs and PRP may be more effective for accelerating diabetic ulcer healing remains unclear. Objective: We investigated the efficacy of BMSCs and PRP for the repair of refractory wound healing in a diabetic rat model. Methods: Forty-eight rats with diabetes mellitus induced by streptozotocin were divided into four groups: treatment with BMSCs plus PRP, BMSCs alone, PRP alone, phosphate buffered saline. The rate of wound closure was quantified. A histopathological study was conducted regarding wound depth and the skin edge at 7, 14, and 28 days after surgery. Results: Wound healing rates were significantly higher in the BMSC plus PRP group than in the other groups. The immunohistochemistry results showed that the expression of platelet/endothelial cell adhesion molecule 1, proliferating cell nuclear antigen, and transforming growth factor-β1 increased significantly in the BMSC plus PRP group compared to the other treatment groups. On day 7, CD68 expression increased significantly in the wounds of the BMSC plus PRP group, but decreased markedly at day 14 compared to the controls. Conclusion: The combination of BMSCs and PRP aids diabetic wound repair and regeneration. (Ann Dermatol 26(1) 1∼10, 2014)

Human Papillomavirus Vaccine Awareness, Acceptability, and Decision-Making Factors among Chinese College Students

Wang, Shao-Ming,Zhang, Shao-Kai,Pan, Xiong-Fei,Ren, Ze-Fang,Yang, Chun-Xia,Wang, Zeng-Zhen,Gao, Xiao-Hong,Li, Man,Zheng, Quan-Qing,Ma, Wei,Zhao, Fang-Hui,Qiao, You-Lin,Sivasubramaniam, Priya Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: College students are recommended as the target groups for catch-up human papillomavirus (HPV) vaccination. Systematical exploration of awareness, acceptability, and decision-making factors of HPV vaccination among Chinese college students has been limited. Materials and Methods: A multi-center survey was conducted in mainland China between November 2011 and May 2012. College students aged 18-22 years were stratified by their grade, gender, and major for sampling. Socio-demographic and HPV-related information such as knowledge, perceptions, acceptability, and attitudes were collected through a questionnaire. Results: A total of 3,497 undergraduates completed the questionnaire, among which 1,686 were males. The acceptability of the HPV vaccine was high (70.8%). Undergraduates from high-level universities, at lower grade, or with greater prior knowledge of HPV vaccines showed higher acceptability of HPV vaccination ($p_{trend}$ <0.001). Additionally, undergraduates with vaccination experience outside the National Expanded Program on Immunization (OR=1.29; 95%CI: 1.10-1.51) or fear of HPV-related diseases (OR=2.79; 95%CI: 2.28-3.41) were more willing to accept HPV vaccination. General knowledge of HPV vaccine was low among undergraduates, and safety was a major concern (71.05%). The majority of students wished to pay less than 300RMB for HPV vaccine and chose the Chinese Center for Disease Control and Prevention as the most appropriate venue for vaccination. Conclusions: Although most undergraduates demonstrate positive attitudes towards HPV vaccination, challenges pertaining to introduction exist in China. Corresponding proactive education and governmental subsidy to do so are urgently needed by this age-group population. Suggestions and potential strategies indicated may help shape the future HPV vaccination program in China.

A Novel Form Analysis Method Considering Pretension Process for Suspen-dome Structures

Zhen Zhou,Yu-long Feng,Shao-ping Meng,Jing Wu 대한토목학회 2014 KSCE JOURNAL OF CIVIL ENGINEERING Vol.18 No.5

Suspen-dome is a kind of new prestressed space grid structure which is formed by combing a single-layer reticulated shell andtensegrity system. For the existence of lower flexible cable-strut system, form analysis is crucial for the mechanical performance ofsuspen-domes. This paper develops a novel form analysis method considering the influence of pretension process for suspen-domestructures. Some definitions of form analysis for suspen-dome structures are first expounded. Then an iterative method is presentedfor form analysis, and a sequential analysis method is proposed for pretension process simulation. By combining these two methods,a form analysis method considering pretension process is constructed for suspen-dome structures. Two examples are employed toverify the proposed method. Numerical example results show that both the error of nodal coordinates and cable forces can stablyconverge to set tolerances. According to a scheduled pretension scheme, form analysis results can accurately achieve the expectedinitial state. Engineering example results show that without considering the influence of pretension process, the form analysis wouldbe error and the final state after all cables tensioned deviates greatly from the expected initial state. However, this can be achievedaccurately through the proposed method in this paper.

Longitudinal anti-cracking analysis for post-tensioned voided slab bridges

Zhen Zhou,Shao-ping Meng,Zhao Liu 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.43 No.4

Post-tensioned concrete voided slab girders are widely used in highway bridge constructions. To obtain greater section hollow rate and reduce the self-weight, the plate thickness of slab girders are designed to be small with the adoption of flat anchorage system. Since large prestress is applied to the anchor end section, it was found that longitudinal shear cracks are easy to occur along the voided slab girder. The reason is the existence of great shearing effect at the junction area between web and bottom (top) plate in the anchor end section. This paper focuses on the longitudinal anti-cracking problem at the anchor end of post-tensioned concrete voided slab girders. Two possible models for longitudinal anticracking analysis are proposed. Differential element analysis method is adopted to derive the solving formula of the critical cracking state, and then the practical analysis method for longitudinal anti-cracking is established. The influence of some factors on the longitudinal anti-cracking ability is studied. Results show that the section dimensions (thickness of bottom, web and top plate) and prestress eccentricity on web plate are the main factors that influence the anti-cracking ability. Moreover, the proposed method is applied into three engineering examples to make longitudinal anti-cracking verification for the girders. According to the verification results, the design improvements for these girders are determined.

Pretension process control based on cable force observation values for prestressed space grid structures

Zhen Zhou,Shao-ping Meng,Jing Wu 국제구조공학회 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.34 No.6

Pointing to the design requirement of prestressed space grid structure being the target cable force, the pretension scheme decision analysis method is studied when there’s great difference between structural actual state and the analytical model. Based on recursive formulation of cable forces, the simulative recursive system for pretension process is established from the systematic viewpoint, including four kinds of parameters, i.e., system initial value (structural initial state), system input value (tensioning control force scheme), system state parameters (influence matrix of cable forces), system output value (pretension accomplishment). The system controllability depends on the system state parameters. Based on cable force observation values, the influence matrix for system state parameters can be calculated, making the system controllable. Next, the pretension scheme decision method based on cable force observation values can be formed on the basis of iterative calculation for recursive system. In this way, the tensioning control force scheme that can meet the design requirement when next cyclic supplemental tension finished is obtained. Engineering example analysis results show that the proposed method in this paper can reduce a lot of cyclic tensioning work and meanwhile the design requirement can be met.

Semi-active control on long-span reticulated steel structures using MR dampers under multi-dimensional earthquake excitations

Zhen Zhou,Shao-ping Meng,Jing Wu,Yong Zhao 국제구조공학회 2012 Smart Structures and Systems, An International Jou Vol.10 No.6

This paper focuses on the vibration control of long-span reticulated steel structures under multidimensional earthquake excitation. The control system and strategy are constructed based on Magneto- Rheological (MR) dampers. The LQR and Hrovat controlling algorithm is adopted to determine optimal MR damping force, while the modified Bingham model (MBM) and inverse neural network (INN) is proposed to solve the real-time controlling current. Three typical long-span reticulated structural systems are detailedly analyzed, including the double-layer cylindrical reticulated shell, single-layer spherical reticulated shell, and cable suspended arch-truss structure. Results show that the proposed control strategy can reduce the displacement and acceleration effectively for three typical structural systems. The displacement control effect under the earthquake excitation with different PGA is similar, while for the cable suspended arch-truss, the acceleration control effect increase distinctly with the earthquake excitation intensity. Moreover, for the cable suspended arch-truss, the strand stress variation can also be effectively reduced by the MR dampers, which is very important for this kind of structure to ensure that the cable would not be destroyed or relaxed.

Downregulated MicroRNA-133a in Gastric Juice as a Clinicopathological Biomarker for Gastric Cancer Screening

Shao, Juan,Fang, Peng-Hua,He, Biao,Guo, Li-Li,Shi, Ming-Yi,Zhu, Yan,Bo, Ping,Zhang, Zhen-Wen Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5

Circulatory miR-133a is a marker shared by several types of cancer. In this study we evaluated the feasibility of using miR-133a levels in gastric juice to screen for gastric cancer. A total of 204 samples of gastric juice and mucosa from gastric cancer, atrophic gastritis, gastric ulcer, superficial gastritis and healthy cases were collected by gastroscopy. The results showed that miR-133a levels in gastric juice and carcinoma tissues of patients with gastric cancer were significantly downregulated and positively correlated. Moreover, miR-133a in gastric juice has high operability, high reliability, high sensitivity, high specificity and relative stability, fit for clinical diagnosis of gastric cancer.

Mechanical analysis for prestressed concrete containment vessels under loss of coolant accident

Zhen Zhou,Chang Wu,Shao-ping Meng,Jing Wu 사단법인 한국계산역학회 2014 Computers and Concrete, An International Journal Vol.14 No.2

LOCA (Loss Of Coolant Accident) is one of the most important utmost accidents for Prestressed Concrete Containment Vessel (PCCV) due to its coupled effect of high temperature and inner pressure. In this paper, heat conduction analysis is used to obtain the LOCA temperature distribution of PCCV. Then the elastic internal force of PCCV under LOCA temperature is analyzed by using both simplified theoretical method and FEM (finite element methods) method. Considering the coupled effect of LOCA temperature, a nonlinear elasto-plasitic analysis is conducted for PCCV under utmost internal pressure considering three failure criteria. Results show that the LOCA temperature distribution is strongly nonlinear along the shell thickness at the early time; the moment result of simplified analysis is well coincident with the one of numerical analysis at weak constraint area; while in the strong constrained area, the value of moments and membrane forces fluctuate dramatically; the simplified and numerical analysis both show that the maximum moment occurs at 6hrs after LOCA.; the strain of PCCV under LOCA temperature is larger than the one of no temperature under elasto-plastic analysis; the LOCA temperature of 6hrs has the greatest influence on the ultimate bearing capacity with 8.43% decrease for failure criteria 1 and 2.65% decrease for failure criteria 3.

Wind-Induced Vibration Responses of Prestressed Double-Layered Spherical Latticed Shells

Zhen Zhou,Zhi-ming Li,Shao-ping Meng,Jing Wu 한국강구조학회 2011 International Journal of Steel Structures Vol.11 No.2

This paper focuses on the wind-induced vibration response of prestressed double-layered spherical latticed shell (PDSLS)structures by adopting time-domain analysis method. Welch spectrum analysis method is used to make precision evaluation of power spectrum of fluctuating wind speed time history simulated by weighted amplitude wavelet superposition (WAWS)method and linear filtering method of auto-regression (AR) model. Results show that the two methods produce little precision difference, but AR method is far more efficient than WAWS and is more suitable for wind speed simulation of PDSLSs. The effect of various parameters on the wind-induced vibration response of PDSLS structures are comprehensively investigated,including rise-span ratio, span, shell thickness, elastic constraint stiffness, prestress value, with or without cables and cable layout scheme. Results show that rise-span ratio and span are the major factors that affect wind-induced vibration response of PDSLSs. When cables are set, the wind vibration coefficient of nodal vertical displacement becomes smaller and more equally distributed, which demonstrates that PDSLSs are less sensitive to fluctuating wind effect than common latticed shell structures without cables. Finally, based on the envelopment concept and with the maximum dynamic and average wind-induced displacement responses as control indicators, the calculating method for global wind vibration coefficient (GWVC) of PDSLSs is proposed and the value with usual design parameters is given. Meanwhile, when the structure is made static analysis by means of the equivalent static wind load obtained from GWVC, the obtained internal member force response is relatively accordant with the actual response got from time-history analysis, and is a little safer.

Stability Analysis of Prestressed Space Truss Structures Based on the Imperfect Truss Element

Zhen Zhou,Shao-ping Meng,Jing Wu 한국강구조학회 2009 International Journal of Steel Structures Vol.9 No.3

Prestressed space truss structures have been widely used in various public buildings in recent years. However, because of its large span, the length of its members also increases. Therefore the member initial curvature will probably affect the stability of the structure. This paper focuses on the truss element with initial geometrical imperfection under high axial load. The relation between axial force and deformation, the expression of axial tangent stiffness, and the concept of initial curvature coefficient for truss element are established. With the example of prestressed cable-stayed arch-truss structure, the influence of member initial curvature on nonlinear stability performance of the structure under various prestress values is studied. The result indicates that member initial curvature has little influence on global stiffness of the structure before buckling, but has great influence on the performance of the structure after buckling and on limit load of the structure. Under different prestress values, the limit load–member initial curvature curves are parallel and almost linear. The proposed method in this paper can be widely applied into nonlinear stability analysis of various prestressed space truss structures with large span, so that the influence of member initial curvature on structural stability can be estimated. Prestressed space truss structures have been widely used in various public buildings in recent years. However, because of its large span, the length of its members also increases. Therefore the member initial curvature will probably affect the stability of the structure. This paper focuses on the truss element with initial geometrical imperfection under high axial load. The relation between axial force and deformation, the expression of axial tangent stiffness, and the concept of initial curvature coefficient for truss element are established. With the example of prestressed cable-stayed arch-truss structure, the influence of member initial curvature on nonlinear stability performance of the structure under various prestress values is studied. The result indicates that member initial curvature has little influence on global stiffness of the structure before buckling, but has great influence on the performance of the structure after buckling and on limit load of the structure. Under different prestress values, the limit load–member initial curvature curves are parallel and almost linear. The proposed method in this paper can be widely applied into nonlinear stability analysis of various prestressed space truss structures with large span, so that the influence of member initial curvature on structural stability can be estimated.