Flooding tolerance evaluation of corn inbred lines during early vegetative stages for adaptation to paddy field soil

Seonghyu Shin(신성휴),Jiyoung Shon(손지영),Shingu Kang(강신구),Kang Bo Shim(심강보),Woonho Yang(양운호) 한국토양비료학회 2015 한국토양비료학회 학술발표회 초록집 Vol.2015 No.12

Mapping of SSR markers closely linked to a bean rust resistant gene in common bean following bulked segregant analysis with whole genome SNP genotyping

Seonghyu Shin,Marcial Pastor-Corrales,Qijiang Song,Gaofeng Jia,Miok Woo,Perry Cregan 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

Recently whole genome SNP genotyping has been used to do association analysis and to map a gene of interest. Here we report application of bulked segregant analysis(BSA) using Infinium HD assay with ‘BARC Bean6K_3’, a SNP genotyping beadchip containing 5,399 SNPs for common bean to locate a target gene. We used BSA using Infinium HD assay was performed to find the candidate region of a single dominant rust resistant gene in PI310762, a common bean cultivar. And SSR markers were identified and mapped on the candidate region using F2 population derived from the cross of susceptible Pinto114 x resistant PI310762. BSA revealed the candidate region of the resistant gene is on chromosome 4 where we developed nine SSR markers. Three SSR markers (beanssr1170, beanssr1168, and beanssr1167) of them appeared closely linked to the resistant gene which is located between beanssr1167 at 0.1cM and beanssr1170 at 0.5cM on chromosome 4. This study showed BSA using high-throughput whole genome SNP genotyping is a very fast and efficient method to locate a gene of interest on chromosome.

Evaluation of Waterlogging Tolerance with the Degree of Foliar Senescence at Early Vegetative Stage of Maize

Seonghyu Shin,Seong-Guk Kim,Gun Ho Jung,Chung-Guk Kim,Beom-Young Son,Jeong Tae Kim,Sang Gon Kim,Woonho Yang,권영업,Kang-Bo Shim,우미옥 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.5

Maize is highly susceptible to waterlogging, which is becoming one of worldwide abiotic threats in many agricultural areas. This study was evaluated to establish the screening method and to find tolerant maize genotypes. Six Korean maize inbred lines were subjected to waterlogging at V3 for 15 days using a big size pot with single maize plant (big pot method) and a box containing 31 maize plants at a time (box method). The degree of foliar senescence and the number of senescent leaves were better indicators for selecting waterlogging tolerant maize genotypes than SPAD value and plant height. The degree of foliar senescence revealed that KS124, KS140, and KS141 are tolerant, and KS85 is susceptible to waterlogging at the early growth stage. These responses of foliar senescence were in clear accordance with those of plant grain yield, which was supported by stress tolerance index for grain yield. The box method also showed the similar response of foliar senescence to the big pot method. Therefore, this box method based on foliar senescence may be simple and efficient for large-scale screening of maize germplasm against waterlogging stress. It was concluded that foliar senescence can be a good indicator for selecting tolerant maize genotypes against waterlogging at the early growth stage.

Differentially expressed proteins between two Korean inbred lines under drought stress at vegetative stage

Sang Gon Kim,Seonghyu Shin,Hwan Hee Bae,Jin-Seok Lee,Jung-Tae Kim,Min Jung Seo,Beom-Young Son,Jeom Ho Lee,Seong-Bum Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Understanding the response of a crop to water deficiency is the first step towards breeding drought-tolerant varieties. In this study, inbred maize (Zea mays L.) lines KS140 and KS141 were subjected to drought stress by withholding water for 10 days at the V5 or V6 leaf stage. Water-deficient plants experienced a decrease in relative leaf water content, stomatal conductance, net CO2 assimilation rate, and water use efficiency compared to well-watered plants. This was accompanied by a decrease in the relative leaf water content that resulted in severe growth retardation in KS140 and KS141. However, leaf chlorophyll content in KS140 was unchanged. To understand the proteome dynamics during the 10-day drought stress in maize leaves, comparative proteome analysis was carried out between the well-watered and water-withheld leaves. Differential expression was observed for 29 protein spots from KS140 and 14 protein spots from KS141, and these were identified using MALDI-TOF mass spectrometry. Among identified proteins, metabolism and stress related proteins were highly were increased by drought stress. This study provides a protein profile of a Korean maize inbred line during drought stress, which will be valuable for future studies of the molecular mechanisms underlying drought resistance and for development of selective breeding markers for drought tolerance in maize.

[CE-0004] Development of waterlogging tolerance corn variety using wild corn Teosinte

Jae-Han Son(Jae-Han Son),Young-Sam Go(Young-Sam Go),Hwan-Hee Bae(Hwan-Hee Bae),Beom-Young Son(Beom-Young Son),Seonghyu Shin(Seonghyu Shin),Tae-Wook Jung(Tae-Wook Jung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Seven Days of Consecutive Shade during the Kernel Filling Stages Caused Irreparable Yield Reduction in Corn (Zea mays L.)

Sang Gon Kim,Seonghyu Shin,Gun-Ho Jung,Seong-Guk Kim,Chung-Guk Kim,Mi-Ok Woo,Min Ju Lee,Jin-Seok Lee,Beom-Young Son,Woon-Ho Yang,Young-up Kwon,Kang-Bo Shim 한국작물학회 2016 한국작물학회지 Vol.61 No.3

In monsoon climates, persistent shade is a troublesome weather condition with an impact on the growth and yield of corn (Zea mays L.). We imposed 7, 14, 21, and 28 days of consecutive shade (CS) on Gwangpyeongok and P3394 corn hybrids at the beginning of the kernel filling stages. Shade had little impact on leaf area and dry matter accumulation in the stem and leaves. However, dry matter accumulation in the ear was severely reduced by approximately 28% and 53% after 14 and 28 days of CS, respectively. For the components of grain yield, 7 and 14 days of shade did irreparable damage to the number of filled kernels, the kernel number per ear row, and the percent of filled kernels, but did little damage or reversible damage after removal of the shade to the 100-grain weight and the row number per ear. Shade significantly reduced the relative growth rate (RGR) due to a decrease in the net assimilation rate (NAR). These results suggest that source activity limitation by shade during the kernel filling stages leads to the inhibition of sink activity and size. The yield of biomass, ear, and grain logistically declined as the length of CS increased. Probit analysis revealed that the number of days of CS needed to cause 25% and 50% reductions in grain yield were 3.7 and 23.1, respectively. These results suggest that the plant yield loss induced by shade at the beginning of the kernel filling stages is mainly achieved within the first 7 days of consecutive shade.

Developing PCR-Based SNP Markers for Distinguishing Korean Waxy Corn F₁ Hybrids

( Sang Gon Kim ),( Jin-seok Lee ),( Seonghyu Shin ),( Hwan Hee Bae ),( Jung-tae Kim ),( Beom-young Son ),( Seong-bum Baek ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.3

Single nucleotide polymorphisms (SNPs) are abundantly and evenly distributed throughout the genomes of most plant species. These markers have become popular for use in genetic research in many crops. SNP markers can be used to screen maize cultivars rapidly during the early growth stages. In this study, to develop additional SNP markers for maize, we chose 20 SNP sites per chromosome from the maizeGDB website (www.maizegdb.org) and designed primers with two base pair mismatches using Primer Designer 4 based on putative SNP sites of the B73 genome sequence. The polymerase chain reaction (PCR) products ranged from 200 to 500 bp in size, whereas no PCR product was detected when the SNP site was present in Korean waxy corn. Using nine Korean commercial F₁ hybrids of waxy corn, including Chalok 1, Chalok 4, Ilmichal, Eolrukchal 1, Heukjinjuchal, Hayanchal 95, Mibaekchal, Mibaek 2, and Miheukchal, we selected 16 primer sets showing clear bands or no bands. Based on cluster analysis, we confirmed that the nine Korean waxy corn hybrids could clearly be distinguished. The SNP marker sets are easy to utilize through simple PCR and agarose gel electrophoresis. These results suggest that analysis using the SNP marker set designed in this study would be faster, cheaper, and more reproducible than that using other genotyping tools, such as cleaved amplified polymorphic sequence markers, which require the use of restriction enzymes.

옥수수 생육 초기 과습 처리에 따른 효소활성 변화

김정태, 손범영, 신성휴, 이진석, 배환희, 김상곤, 정광호, 이재은, 정건호, 우관식, 백성범, 권영업 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

This study was carried out to evaluate the change of four type enzyme (ADH activity, LDH activity, α-amylase activity, β-amylase activity) activity under the flooding condition at maize seedling. At maize seeding, two hybrid maize (Kwangpyeongok and Gangdaok) showed that plant height and fresh weight was decreased when flooding periods increased. Especially, plant height and fresh weight of Gangdaok was the rapidly decline than these of Kwanpyeonok. In analyse of enzyme, ADH and α-amylase activity showed the difference of enzyme activity betweem Kwangpyeongok and Gangdaok. Kwangpeonok showed that ADH and α-amylase activity under the flooding condition were the high enzyme activity compared as non flooding treatment.

남부산간지에서 봄과 여름 조사료 귀리의 파종과 수확 시기에 따른 조사료 품질과 생산성 변화

신성휴,이현정,구자환,박명렬,라경윤,김병주,Shin, Seonghyu,Lee, Hyunjung,Ku, Jahwan,Park, Myungryeong,Rha, Kyungyoon,Kim, Byeongju 한국작물학회 2021 한국작물학회지 Vol.66 No.2

본 연구는 조사료 연중생산이 불리한 남부산간지역(전북 장수군)에서 봄과 여름에 재배하는 조사료 귀리의 적정 파종과 수확시기를 구명하고자 하였다. 시험품종은 여름 재배용 국산 조사료 귀리 품종 '하이스피드'와 '다크호스'이었다. 남부산간지에서 봄과 여름 조사료 귀리의 적정 파종기와 수확시기를 구명하기 위하여 파종과 수확시기를 달리하여 각각 재배하고 조사료 수량과 품질 변화 양상을 분석하였다. 시험장소는 전북 장수군 소재 농가 논 포장이었다. 2015년과 2016년에 2년간 수행한 연구결과를 요약하면 다음과 같다. 1. 조사료 귀리를 2월 하순, 3월 상순과 중순에 파종하였을 때, 출수까지 적산온도가 각각 690℃, 724℃, 743℃이었고, 8월 상순, 중순, 하순에 파종하였을 때, 출수까지 적산온도는 각각 1,449℃, 1,091℃, 888℃이었다. 2. 조사료 귀리 품종(하이스피드, 다크호스)을 2월 하순부터 9일 간격으로 3월 중순까지 파종하면 출수기는 모두 5월 10일~5월 16일이었고 평균 3일 정도만 늦었고, 8월 상순부터 10일 간격으로 8월 하순까지 파종하면 출수기는 모두 10월 13일~10월 15일이었고 평균 1~2일 정도만 늦어지는 것으로 나타났다. 특히 9월 상순에 파종한 조사료 귀리는 출수하지 않았다. 3. 봄과 여름 조사료 귀리의 건물수량과 품질은 모두 파종 시기에 유의미한 영향을 받지 않았다. 4. 봄 조사료 귀리의 조단백 함량은 5월 하순에 수확한 것이 12.0%로 가장 높았고 그 이후 수확시기가 10일씩 늦을수록 급격히 감소하여 6월 상순에는 8.2%, 6월 중순에는 단백질 함량이 6.5%까지 감소하였고, TDN 함량(59~62%)도 비슷한 경향이었지만 감소폭은 작았다. 5. 여름 조사료 귀리는 파종시기가 늦을수록 단백질과 TDN 함량이 증가하는 경향이었고, 10월 하순부터 11월 중순까지 10일 간격으로 수확한 조사료 귀리의 조단백 함량이 8.4~8.7%, TDN 함량이 59.0~60.1% 정도이었다. 6. 봄 조사료 귀리는 6월 상순과 6월 중순에 수확한 건물수량이 각각 18.7톤/ha와 19.5톤/ha으로 5월 하순에 수확한 것보다 각각 83%와 91% 정도 높았다. 7. 여름 조사료 귀리는 11월 상순과 중순에 수확한 건물수량이 각각 12.5톤/ha와 12.1톤/ha으로 10월 하순에 수확한 것보다 각각 75%와 71% 정도 높았다. 8. 봄과 여름 귀리의 조사료 수량은 출수 이후 수확까지 적산온도와 높은 정의 상관관계를 나타내었다. 9. 봄과 여름 조사료 귀리의 건물수량과 품질을 고려하였을 때, 남부산간지에서 조사료 귀리를 봄에 재배할 경우에 3월 15일까지 파종하고 6월 10일경에 수확하고, 여름에 재배할 경우에는 8월 25일경까지 파종하고 11월 상순 이후 수확하면 단백질 함량이 8% 이상인 고품질 조사료의 최대 수량을 얻을 수 있는 것으로 판단되었다. Oats (Avena sativa L.) represent a good forage crop for cultivation in regions with short growing periods and/or cool weather, such as the mountainous areas of southern Korea. In this study, using the Korean elite summer oat varieties 'High speed' and 'Dark horse', we aimed to determine the optimal time to plant and harvest forage oats seeded in spring and summer in a mountainous area. Seeds were planted three times from late February and early August at 9- or 10-days intervals, respectively, and plants were harvested three times from late May to October at 10-day intervals. The experiment was carried out in an upland field (Jangsu-gun Jeonbuk) in 2015 and 2016. We investigated the changes in forage yield (FY) and quality [crude protein (CP) and total digestible nutrient (TDN) contents] based on the time of planting and harvest. Neither the forage quality nor yield of either spring and summer oats was significantly influenced by the time of planting. The CP of spring oats harvested three times at 10-day intervals from late May was 12.0%, 8.2%, and 6.5%, thereby indicating a reduction with a delay in the time of harvest. In summer oats, CP ranged from 8.4% to 8.7%, although unlike CP in spring oats, was not significantly influenced by the time of harvest. For both forage types, harvest time had no significant effect on TDN. The FY of spring oats harvested in late May and early and mid-June was 10.2, 18.7, and 19.5 ton ha^-1, respectively, with that of oats harvested on the latter two dates being significantly increased by 83% and 91%, respectively, compared with that in late May. Similarly, the FY of spring oats harvested in late October and early and mid-November was 7.1, 12.5, and 12.1 ton ha^-1, respectively, with that of oats harvested on the latter two dates being significantly increased by 75% and 71%, respectively, compared with that in late October. Taking into consideration forage yield and quality (not less than 8% CP), it would be profitable to plant spring oats in the mountainous areas of southern Korea until March 15 and harvest around June 10, whereas summer oats could be beneficially planted until August 25 and harvested from early November.

Measuring antioxidant activity in yellow corn (Zea mays L.) inbreds from three different geographic regions

Bae Hwan-Hee,이기범,Go Young Sam,Ha Jun Young,Choi Yuchan,Son Jae-Han,Shin Seonghyu,Jung Tae-Wook,Lee Sungwoo 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.S

Corn (Zea mays L.) provides a major source of calories for human consumption and therefore, the nutritional components of corn have a large impact on human health. For example, corn kernels contain antioxidants, such as polyphenols (including anthocyanins and other flavonoids) and carotenoids. Such compounds represent useful targets for biofortification breeding. In this study, we used 34 corn inbred lines from three different regions (East Asia, Southern Asia, and subtropical regions) and 11 F1 hybrids derived from the inbreds to investigate antioxidant activity in yellow corn. We compared different methods for measuring antioxidant activity to test their consistency and to determine whether color could be used as an indicator of antioxidant activity. We also measured carotenoid levels in yellow corn. No difference in antioxidant activity was detected between inbred corn lines from temperate vs. tropical regions. We determined that carotenoid is a major contributor to antioxidant activity in yellow corn and that kernel color, especially yellowness, could be used as an indicator of antioxidant activity in yellow corn. These findings lay the foundation for the biofortification of yellow corn by providing information about the correlations among kernel color, carotenoid contents, and antioxidant activity and by identifying an easy method to assess antioxidant activity in yellow corn.