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Shin, D.Y.,Sung Kang, H.,Kim, G.Y.,Kim, W.J.,Yoo, Y.H.,Choi, Y.H. Masson Pub. USA, Inc 2013 Biomedicine & pharmacotherapy Vol.67 No.4
Decitabine (5-aza-2'-deoxycytidine), an inhibitor of DNA methyltransferases, has a wide range of anti-metabolic and anti-cancer activities. Decitabine also induces cell cycle arrest at G2/M phase and apoptosis in human cancer cells. However, the cellular and molecular mechanisms of this cell cycle arrest are poorly understood. In the present study, we investigated the roles of the tumor suppressor p53 and the cyclin-dependent kinase (Cdk) inhibitor p21 following decitabine-induced G2/M arrest in human cancer cells. DNA flow cytometric analyses indicated that decitabine induced a G2/M arrest in AGS gastric and A549 lung carcinoma cell lines, which have wild type p53. Western blot analyses using whole cell lysates from AGS cells demonstrated that decitabine treatment did not change the steady-state level of Cdks and Cdk inhibitor p27, but it partially inhibited expression of cyclin A, cyclin B1, and Cdc25C proteins. However, similar results were found using the A549 cell line, where decitabine induced a dramatic up-regulation of both p53 and p21 expression, and the increased levels of p21 were associated with increased binding of p21 with Cdks, cyclin A, and cyclin B1. Knockdown of p53 by small interfering RNA (siRNA) markedly abolished p53 induction by decitabine in AGS cells, yet p53 siRNA had no attenuating effect on p21 induction. In addition, depletion of p21 expression with siRNA, but not p53, significantly attenuated decitabine-induced G2/M arrest. We also observed that decitabine strongly induced G2/M arrest associated with p21 induction in both p53 allele-null (-/-) HCT116 and wild type p53 (+/+) HCT116 cell lines. Therefore, our data indicated that p21 plays a crucial role in decitabine-induced G2/M arrest and operates in a p53-independent manner.
Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene
Kim, J.Y.,Yoon, D.H.,Park, B.L.,Kim, L.H.,Na, K.J.,Choi, J.G.,Cho, C.Y.,Lee, H.K.,Chung, E.R.,Sang, B.C.,Cheong, I.J.,Oh, S.J.,Shin, Hyoung Doo Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.1
The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5'UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G-854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their frequencies were estimated by EM algorithm. Six haplotypes were constructed with five SNPs and linkage disequilibrium coefficients (|D'|) between SNP pairs were also calculated. The information on SNPs and haplotypes in IGFBP3 gene could be useful for genetic studies of this gene.
위상형 CGH(computer generated hologram)를 이용한 비구면 파면 및 Bessel beam 제작
성기영,경천수,이영락,이정훈,이상조,곽종훈 영남대학교 기초과학연구소 2000 基礎科學硏究 Vol.20 No.-
We have designed and fabricated computer generated holograms(CGH) to generate ashepheric wavefront and Bessel beam. Designed CGH is recorded onto the photopolymer which is used as a volume hologram material mating CGHOE(computer generated holographic optical element). To investigate properties of fabricated CGHOE, intensities of Bessel beam are measured as a function of propagation(z-axis) and transverse (x-axis) distances.
農村學生과 都市學生의 運動適性에 關한 調査硏究 : 運動適性 要因分析을 中心으로
成鍾林,申吉洙,李商鍾 圓光大學校 1973 論文集 Vol.7 No.-
The present study attempted to know the next two things by administering physical fitness tests to students. First, physical fitness for high school boys in rural and urban area. Second, factors attributable to regional differences in fphysical fitness for high school boys. Number of subjects were 274 boy students, 156 of Daw Kwang High School and 118 of Iun Buk Young Sun High School. Results of the study can be summarized as follows. 1. Physique of students in rural area showed bigger than that of students in urban area. 2. Development of the body continued from 16 years of age to 19 years ooage. However, a little development showed between age of 19 and age of 20. 3. Strength of muscles increased from 16 years of age to 20 years of age. Measurement scores indicated that rural students were stronger than urban students. 4. Flexibliity decreased as students grew older. Urban students showed high tests scores in flexibility than rural students. 5. Rural students showed much better in cardio-pulmonary function than urban students. 6. Urban students showed better in agility and starting power than rural stul dents. 7. In analizing results above, rural students showed better tests scores in physique, cardio-pulmonary function, and muscle strength. On the other hand, Urban students showed better tests scores in agility, starting power, and balance than rural students.
녹용분말 급여가 성장기별 흰쥐의 성장 및 장기발육에 미치는 효과
성하균,김동균,신형태 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5
The velvet antler of Korean sika deer has been used to the important resources for human health care with ginseng in Korea and Chinese. For studying on biological function of deer velvet being recorded in many ancient literatures, this experiment was conducted to evaluate the effects of powdered velvet antler on growth, feed intake, feed efficiency and intestinal organ development in growing and adult Sprague-Dawley Rats. Experiments were designed by adding amount of powdered velvet antler such as control (non-supplementation), powdered velvet antler Ⅰ (PVA Ⅰ, recommended dos) and powdered velvet antler Ⅱ (PVA Ⅱ, thrice recommended dose). The recommended dose of powdered velvet in this experiment was calculated with metabolic body weight of rats, which based on the recommended amounts for 70㎏ of human. The growths of growing and adult rats generally appeared advantage in PVA supplementation. The final body weight of control, PVA Ⅰ and Ⅱ were respectively 470±39.00g, 478±3033g and 475±22.72g in growing rats, and 485±38.50g, 521±38.67g and 508±34.44g in adult rats. The average daily feed intake were not significantly influenced but the feed efficiency ratios (feed/gain) were improved by PVA supplementation. The feed/gain ratios of control, PVA Ⅰ and Ⅱ were respectively 5.99, 5.47 and 5.54 in growing rats, and 9.04m 7.73 and 8.18 in adult rats. In case of developments of liver, heart, kidney and stomach, we obtained favorable results in both PAC Ⅰ and Ⅱ, but their results were not significantly different according to amount of PVA supplementation. Because liver and heart are important organs in the circulation of blood, their favorable effects suggest that velvet antler may have factors contributing hematopoiesis. Conclusively, supplementation of that velvet antler may have factors contributing hematopoiesis. Conclusively, supplementation of powdered velvet antler resulted in an improved growth, feed efficiency and development of some intestinal organs in growing and adult rat. Even if further investigation of dose in human health care are should be performed, this experiment appeared the best desirable results in PVA Ⅰ, recommended dose of powdered velvet antler.
2종의 엽록소 측정기를 이용한 포장내 벼의 엽록소 함량 분포 측정
성제훈,서상룡,박우풍,정인규,김상철,이충근 한국농업기계학회 2003 바이오시스템공학 Vol.28 No.3
This study was investigated the possibility of measuring chlorophyll in paddy crops using two kinds of chlorophyll meters(SPAD-502, Field Scout). The results of correlation analysis showed the 0.7758(r^2) relationship between the chlorophyll contents of paddy leaves measured by SPAD and spectro-photometer. It indicates that chlorophyll content in paddy crops could be measured using the SPAD. Considering the data of the SPAD and Field Scout, there was significant difference between their chlorophyll contents measured by the SPAD and Field Scout. Likewise, such results were consistent with the corrected data using light intensity. According to the results, it can be concluded that it is difficult to describe the rice chlorophyll measured by the Field Scout more accurately than the SPAD when using one standard spectrum and another spectrum for reflectance measuring. The chlorophyll variance measured at a research institute and a farmer's field revealed that the output of SPAD was more reliable than that of the Field Scout.