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Expression and Secretion of Heterologous Protein in Yeast
Kim, Moo-Kyum,Song, Moo-Young,Yu, Myeong-Hee,Yu, Myeong-Hee,Park, Hee-Moon,Kim, Jinmi The Microbiological Society of Korea 1992 미생물학회지 Vol.30 No.2
To investigate the expression and the secretion of heterologous proteins in yeast, we constructed an yeast secretion vector and produced a human secretory protein, .alpha.-1-antitrypsin (.alpha.-1-AT), from yeast cells. The secretion vector pGAT8 was constructed by inserting the signal sequence of yeast acid phosphatase gene (PH05) into the .alpha.1-AT expression vector pGAT6 which contained .alpha.-1-AT cDNA fused to GAL10-CYC1 promotor. The .alpha.-1-AT was produced efficiently in the yeast cells transformed with plasmid pGAT8, which was onfirmed both by the .alpha.-1-AT activity assay and by the immunoblot method using .alpha.-1-AT antibody. We also showed the secretion of .alpha.-1-AT into the culture media and into the periplasmic space by immunoblot.
連續이온 浮選法을 利用한 重크롬酸이온 含有廢水處理에 關한 硏究
김대겸,박돈희,이승무 연세대학교 산업기술연구소 1978 논문집 Vol.8 No.1
This experimental investigation is presented of the ion ic flotation of dichromate from waste water with a cationic surfactant suchas Dodecyl Dimethyl Benzyl Ammonium (DDBA) Chloride. Dichromate forms a colloidal complex with DDBA ion in a mole ratio of approximately 1:2. Excess surfactant causes the formation of a stable foam to which the complex is adsorbed, providing the separation of dichromate from waste water. The feed streams contain concentations of dichromate (Cr?) ranging from 5 to 50 ppm and feed concentration ratios of surfactant to dichromate ranging from 2.5. to 6 (on a mole base). The effects of feed concentration ratio of surfactant and dichromate, the change of PH, and the positions of feed and reflux, on the removal of dichromate, were investigated. The most efficient operation was carried out by setting the feed concentration ratio of DDBA ion to dichromate ion from 2.5 to 3.5 and the stripping ratio (C?/C?) of 0.47 was than obtained. Decreasing PH of the feed from5 increased the removal of dichromate. Inserting the feed solution into the mid point of foam height produced more collapsed foam streams of dichromate, with slower flow rate of foamate than inserting it into the mid point of solution layer. Positioning reflux inlet abcve H?/H?=0.4 through the foam column increased the flow rate of reflux dichromate.
Lee, Myungjin,Kim, Myung Kyum,Kwon, Mi-Jung,Park, Byeong Deog,Kim, Moo Hoon,Goodfellow, Michael,Lee, Sung-Taik Elsevier 2005 Journal of bioscience and bioengineering Vol.100 No.4
<P>The dynamics of diesel oil biodegradation were previously investigated at initial substrate concentrations of 1000 to 20,000 ppm using <I>Gordonia nitida</I> isolated from wastewater. Following the gas chromatogram profiles of diesel oil degradation, diesel oil with concentrations of up to 15,000 ppm was efficiently degraded by this strain. At a concentrations of 20,000 ppm, however, the degradation by this strain was not effective. The enhancement of the biodegradation of diesel oi1 (at 15,000 and 20,000 ppm) by a synthetic mycolic acid biosurfactant (at 9, 90 and 900 ppm) was also investigated. In <I>G. nitida</I> inoculated cultures, the degradation of diesel oil was enhanced by the biosurfactant. For comparison, diesel oil degradation in batch incubations was measured after the addition of rhamnolipid and other surfactants. Synthetic mycolic acid enhanced the degradation to a greater extent than any other surfactant tested. Additionally, it was demonstrated that the degradation-enhancing property of synthetic mycolic acid was similar to that of rhamnolipid and Tween 80.</P>
Dual Anti-oxidative Effects of Fraxetin Isolated from <i>Fraxinus rhinchophylla</i>
Thuong, Phuong Thien,Pokharel, Yuba Raj,Lee, Moo Yeol,Kim, Sang Kyum,Bae, KiHwan,Su, Nguyen Duy,Oh, Won Keun,Kang, Keon Wook Pharmaceutical Society of Japan 2009 Biological & pharmaceutical bulletin Vol.32 No.9
<P>Atherosclerosis is main cause of arteriosclerosis. The pivotal role of low-density lipoprotein (LDL) oxidation in atherogenesis suggests antioxidants may help prevent cardiovascular disease. <I>Fraxinus rhynchophylla</I> D<SMALL>ENCE</SMALL> (Oleaceae) is a traditional medicinal plant from East Asia. During the course of characterizing potential drug candidates from natural products, we isolated two major coumarins, esculetin and fraxetin and found that fraxetin has dual-antioxidative functions. Low concentrations (1—5 μ<SMALL>M</SMALL>) of fraxetin potently inhibited LDL oxidation induced by metal and free radicals. Moreover, treatment of vascular smooth muscle cells (VSMCs) with higher concentrations (above 30 μ<SMALL>M</SMALL>) of fraxetin significantly increased the protein level of heme oxygenase-1 (HO-1), a key enzyme that inhibits vascular proliferation and atherosclerosis. Subcellular fractionation and reporter gene analysis using an antioxidant response element (ARE) construct revealed that fraxetin increased the level of nuclear factor (NF)-E2-related factor 2 (Nrf2) and reporter activity, and these were associated with the induction of antioxidant enzymes, such as HO-1 and glutathione <I>S</I>-transferase-α. In conclusion, fraxetin has direct protective properties against LDL oxidation at lower concentrations, and higher concentrations of fraxetin induce antioxidant enzymes <I>via</I> Nrf2/ARE activation. These effects suggest potential anti-atherosclerosis effects of <I>Fraxinus rhynchophylla</I> D.</P>
Guillain-Barré syndrome supervening on meningitis in primary Epstein-Barr virus infection
Lee, Jeong-Yoon,Sunwoo, Jun-Sang,Kwon, Kyum-Yil,Lee, Kyung Bok,Ahn, Moo-Young,Roh, Hakjae The Korean Society of Clinical Neurophysiology 2019 Annals of Clinical Neurophysiology Vol.21 No.1
Primary Epstein-Barr virus (EBV) infection can manifest with a broad spectrum of neurological complications. There are only rare reports of Guillain-$Barr{\acute{e}}$ syndrome (GBS) supervening on meningitis in patients with primary EBV infection. Clear evidence of central nervous system infection makes it difficult for the clinicians to consider a diagnosis of GBS. We present a patient with GBS supervening on meningitis in primary EBV infection.