Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

Capsaicin 식이가 당뇨쥐의 대사에 미치는 영향

전성현,박병주 전남대학교 치과대학 2001 구강과학 Vol.13 No.2

The effects of dietary capsaicin--the pungent principle of red papper--on glucose and lipid metabilsm were studied in diabetic rats. Diabetes was induced by intraperitioneal injection of streptozotocin (30 mg/kg body weight) into Wistar female rats weighing up to 200 g. Capsaicin was then used as a dietary supplement at concentrations of 0.014% or 0.028% for a period of 4 weeks. Blood glucose and insulin levels were determined during an intravenous glucose tolerance test. Our result show that dietary capsaicin did not make any difference in the ingested food amounts and body weights of the experimental animals compared to the diabetic control or the control group. At the 4th week, capsaicin (0.028%) fed daibetic rats exhibited significantly decreased blood glucose levels on 0, 10, and 20 minutes after an intravenous glucose loading. There were no significant changes in the plasma concentrations of triglyceride and HDL-cholesterol between various groups, but there were decreasing tendency in the plasma concentrations of LDL-cholesterol, phosholipid, and free fatty acids in capsaicin-fed group. The plasma concentrations of total cholesterol and lipoprotein(a) were significantly decreased in capsaicin-fed group compared to diabetic control group. These results show that dietary capsaicin improves the glucose and lipid metabilsm in diabetes mellitus, suggesting that capsaicin may be useful for the treatment of diabetes and for the interention of diabetic complications.

韓國의 食糧自給을 위한 提言 1980-1990年 計劃

田相浩 경성대학교 1983 부산수산대학 논문집 Vol.4 No.4

임플란트 주위 조직 보존을 위한 임플란트 경부의 디자인에 관한 고찰

김홍준,김지환,김성태,이재훈,박영범 大韓齒科保存學會 2011 Restorative Dentistry & Endodontics Vol.36 No.6

연구 목적: 임플란트 식립 후 변연골 흡수에 따라 임플란트 주위 연조직이 재구성되며, 이에 따라 치료의 예후 및 심미성 등에 영향을 주게 된다. 그러므로 임플란트 경부 주위 골조직 보존을 위한 임플란트 경부에 다양한 디자인이 연구되고 있다. 본 고찰의 목적은 초기 변연골 흡수의 원인과 이에 따른 임플란트 주위의 연조직 변화에 대해 고찰하고, 어떠한 임플란트 경부 디자인이 임플란트 주위 조직의 보존에 유리한 지 알아보고자 한다. 연구 재료 및 방법: Pubmed database에서 임플란트 초기 변연골 흡수의 원인과 관련된 논문과 임플란트 경부의 여러 디자인에 관한 논문을 검색하여 분석하였다. 임플란트 경부 디자인은 one piece implant, two piece implant, internal hex abutment, external hex abutment, taper joint connection, butt joint connection, scalloped design abutment, platform switching concept에 관해 검토하였다. 결과: 초기의 임플란트 주위 조직 보존에 대하여 one piece implant가 two piece implant보다 유리한 것으로 여러 임상적, 실험적 연구가 있다. Two piece implant에서는 internal hex abutment가 external hex abutment보다, taper joint connection가 butt joint connection보다 유리할 것으로 보여진다. Scalloped design abutment에 대해서는 논쟁의 여지가 있어 더 많은 연구가 필요할 것으로 판단된다. Platform switching concept은 그 원인이 명확히 밝혀지지는 않았으나 임상적, 실험적으로 초기 임플란트 주위 조직 보존에 대해 유리한 것으로 판단된다. 결론: 임플란트 경부의 디자인마다 각각의 장단점이 있고 추가적인 연구가 더 필요한 제한이 있지만 현재까지의 선행 연구들을 분석 종합해 보면 초기 임플란트 주위 조직 보존을 고려한다면 가능한 경우 one piece implant가 유리할 것으로 판단되며, 보철적인 문제나 다른 이유로 인하여 two piece implant를 고려할 경우 platform switching concept, internal connection abutment, taper joint connection을 이용하는 것이 임플란트 주위 조직 보존에 좀더 유리할 것으로 사료된다 Purpose: The peri-implant soft tissue is remodeled by the initial marginal bone resorption affecting the prognosis and esthetic result of treatment. Thus various designs on implant neck design are studied to preserve peri-implant bone. The purpose of this study is to review on the causes of initial marginal bone resorption, the configuration of peri-implant soft tissue, and the implant crestal module favorable in preserving peri-implant tissue. Materials and methods: The studies on the causes of initial marginal bone resorption and the implant crestal modules are researched and reviewed using Pubmed database. The implant crestal modules including one piece and two-piece implant, internal and external hex abutment, taper and butt joint connection, scalloped design abutment, and platform switching concept are reviewed. Results: A number of clinical and experimental studies preferred one piece implant to two-piece in preserving initial peri-implant tissue. For two piece implants, internal hex abutment and taper joint connection appear more favorable than external hex abutment and butt joint connection relatively. Controversial issues still exist on scalloped design requiring more studies on it. Although the rationale is not certain, the concept of platform switching seems favorable in preserving initial peri-implant tissue based on clinical and experimental studies. Conclusion: Each implant crestal module contains its own advantages and disadvantages with various controversial issues. In the aspect of preservation of initial peri-implant tissue, however, one-piece implant seems beneficial. In cases when two-piece implant is more appropriate due to prosthodontic concerns or any other problems, the application of platform switching concept, internal connection abutment, and taper joint connection may be favorable for the preservation of peri-implant tissues.

組織의 意思決定過程에 關한 硏究

田相浩 경성대학교 1984 부산수산대학 논문집 Vol.5 No.1

문자영상 紬線化에서의 雜音除去 알고리즘에 관한 연구

전병민,박규태,김상운,이기돈 연세대학교 산업기술연구소 1984 논문집 Vol.16 No.2

In this paper, we study thinning algorithms which are peeling off successive contours of the character image to be thinned while keeping the connectivity of the skeleton in the process. Noise rejection algorithm is proposed for the purpose of removing the common short-comings of thinning algorithms when applied to hand-printed data. The algorithm consists of the three processings: preprocessing, thinning, and postprocessing. The preprocessing smooths down the originally-thick image and the postprocessing leads to the well-groomed skeleton. The noise rejection algorithm is implemented with FORTRAN language and tested through a CYBER170-825 computer system.

조업 조건에 따른 이트륨 옥살레이트 결정의 성장 메카니즘 및 속도

성민현,김우식,김준수 慶熙大學校 材料科學技術硏究所 1998 材料科學技術硏究論集 Vol.11 No.-

Crystal growth of Yttrium oxalate seed crystals in supersaturated solutions has been studied EDTA titration method. Growth rate for Yttrium oxalate crystals was determined from de-supersaturation curve of solution in agitated reactor. Change of growth mechanism and rate of yttrium oxalate crystals on agitation speed and concentration of additive were investigated. Experimental method are suggested to determin the parameters in the simple two-step model and factors which influence the mass-transfer and surface-reaction coefficients are identified. Then the controlling step of crystal growth process is judged from the effectiveness factor. Firstly, growth rate was increased with increasing agitation speed, but exponential constant wasn't change with increasing agitation speed. As this result, linear growth rate were first-order at high supersaturation, that is, growth rate controlled due to mass trasfer step in high supersaturation solution. Finally, studing on effect of additive, growth rate were decreased with increasing concentration of additive, but exponential constant were increased. Then the individual rate constants, kd and kr, were determined from the simple two-step model. In addition, using effectiveness factors, growth rate were measured of the degree of mass-trasfer or surface-reaction control.

진로미결정 문제와 심리적 변인의 관계

김동준, 임성문 忠北大學校 學生生活硏究所 1997 學生生活硏究 Vol.22 No.-

본문참고

소양호 퇴적물에 함유된 인의 존재형태와 용출가능성에 대하여

전상호,박용안 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Fractional composition and mobility of sediment phosphorus in Lake Soyang are investigated. The fractional scheme was made for four chemically defined hposphorus forms as adsorbed phosphorus, non apatite inorganic phosphorus(NAI-P), apatite phosphorus and residual phosphorus. These forms were measured by chemical extraction techmique. The most abundant fraction is residual phosphorus and the secondly abundant form NAI-P, and thirdly apatite phosphorus, and finally adsorbed phosphorus. The most efficient processes of scavening of dissolved phosphours in the lake are biological uptake and chemical complexation. To evaluate the eggects of pH on the mobility of sediment phosphorus, incubation experiments under aerobic and different pHcondition of leaching solution are accomplished. The results show the pH of the leaching solution has strong influence on the release of sediment phosphorus and increases of leaching in high pH aremore apparent in the NAI-P rich sediments than those in the residual P rich sediments.