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Enhanced bone formation by transforming growth factor-β1-releasing collagen/chitosan microgranules
Lee, Jue-Yeon,Kim, Kyoung-Hwa,Shin, Seung-Yoon,Rhyu, In-Chul,Lee, Yong-Moo,Park, Yoon-Jeong,Chung, Chong-Pyoung,Lee, Seung-Jin Wiley Subscription Services, Inc., A Wiley Company 2006 Journal of biomedical materials research. Part A Vol.a76 No.3
<P>Collagen/chitosan composite microgranules were fabricated as bone substitutes for the purpose of obtaining high bone-forming efficacy. The microgranules have the flexibility to fill various types of defect sites with closer packing. The interconnected pores formed spaces between the microgranules, which allowed new bone ingrowth and vascularization. In addition, the transforming growth factor-beta 1 (TGF-β1) was incorporated into the microgranules in order to improve bone-healing efficacy. The collagen/chitosan microgranules were fabricated by dropping a mixed solution into a NaOH/ethanol solution. TGF-β1 was loaded into the collagen/chitosan microgranules by soaking the microgranules in a TGF-β1 solution. Scanning electron microscopy (SEM) observations and experiments examining the release of TGF-β1 from chitosan and the collagen/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules and cell proliferation was evaluated using a dimethylthiazole tetrazolium bromide assay. The differentiated cell function was assessed by measuring the alkaline phosphatase (ALPase) activity as well as detecting an osteocalcin assay. The in vivo bone-regeneration experiments were performed using a rabbit calvarial defect model. TGF-β1 was released from the collagen/chitosan microgranules at a therapeutic concentration for 4 weeks. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer manner. The proliferation of the osteoblasts on the TGF-β1-loaded microgranules was the highest among the different types of microgranules tested. The ALPase activity and osteocalcin level of all the samples increased during the culture period, and the TGF-β1-loaded microgranules had a significantly higher ALPase activity and osteocalcin content than the other microgranules. The TGF-β1-loaded microgranules demonstrated a higher bone-regenerative capacity in the rabbit calvarial defects after 4 weeks than the TGF-β1-unloaded microgranules. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</P>
Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials
Lee, Jue-Yeon,Nam, Sung-Heon,Im, Su-Yeon,Park, Yoon-Jeong,Lee, Yong-Moo,Seol, Yang-Jo,Chung, Chong-Pyoung,Lee, Seung-Jin 梨花女子大學校 藥學硏究所 2002 藥學硏究論文集 Vol.- No.11
For the pulpose of obtaining high bone forming efficacy. developlment of chitosan was attempted as a tool useful as ascaffolding device. Porous chitosan matrices, chitosan-poiy(L-lactide) (PLLA) composite matrtices and chitosan coated onPLLA matrices were dealt with in this research. Porous chitosan matrix was fabricated by freeze-drying and cross-linkingaqueous chitosan solution. Porous chitosan matrix combined with ceramics and constituents of extracellular matrices wereprepared and examined for their bone regenerative potential. Composite porous matrix of chitosall-PLLA was prepared bymixing polyiactide with chitosan and freeze-drying. Al1 chitosan based devices demonstrated improved bone formingcapacity by increasing mechanical stability and biocompatibility. Release of platelet-derived growth factor-BB (PDGf-BH )from these matrices exerted significant osteoinductive effect in addition to the higf esteocdElducting capacity of the porouschitosan matrices. The hydrophobic surface of PLLA matrices was modified by chitosan to enhance cell affinity andwettability, The chitosan coafed PLLA matrix induced increased osteoblast attachment as compared with intact PLLAsurface. Overall results in this study demonstrated the usefulness of chitosan as drug releasing scaffolds and as modificationtools for currently used biomaterials to enhance tissue regeneration efficacy. These results may expand the feasibilitr ofcombinatfve strategy of controlled locai drug delivery concept and tissue engineered bone formation in reconstructivetherapy in the field of periodontics, orthopedics and plastic surgery.
Biological effects of a root conditioning agent for dentin surface modification in vitro
Lee, Jue-Yeon,Seol, Yang-Jo,Park, Jang-Ryul,Park, Yoon-Jeong,Chung, Chong-Pyoung Korean Academy of Periodontology 2010 Journal of Periodontal & Implant Science Vol.40 No.6
Purpose: Connective tissue reattachment to periodontally damaged root surfaces is one of the most important goals of periodontal therapy. The aim of this study was to develop a root conditioning agent that can demineralize and detoxify the infected root surface. Methods: Dentin slices obtained from human teeth were treated with a novel root planing agent for 2 minutes and then washed with phosphate-buffered saline. Smear layer removal and type I collagen exposure were observed by scanning electron microscopy (SEM) and type I collagen immunostaining, respectively. Cell attachment and lipopolysaccharides (LPS) removal demonstrated the efficiency of the root conditioning agent. Results: SEM revealed that the smear layer was entirely removed and the dentinal tubules were opened by the experimental gel. Type I collagen was exposed on the surfaces of the dentin slices treated by the experimental gel, which were compared with dentin treated with other root planing agents. Dentin slices treated with the experimental gel showed the highest number of attached fibroblasts and flattened cell morphology. The agar diffusion assay demonstrated that the experimental gel also has effective antimicrobial activity. Escherichia coli LPS were effectively removed from well plates by the experimental gel. Conclusions: These results demonstrated that this experimental gel is a useful tool for root conditioning of infected root surfaces and can also be applied for detoxification of ailing implant surface threads.
미국선교치과의사 쉐플리와 세브란스연합의학교 치과학교실 개설의 역사적 의의
이주연(Jue Yeon Lee),권호근(Kwon Ho Keun),박형우(Hyoung Woo Park) 대한치과의사협회 2015 대한치과의사협회지 Vol.53 No.11
This article discusses accomplishments and historical implications of American missionary dentist W.J. Scheifley and the first Korean dental department, which was established in 1915 in Korea. W.J. Scheifley, with Christian service mind and mission as a dentist, applied to American Protestantic missionary dentist overseas . The dental department in the Severance Union Medical College introduced the scientistic dental education of America, facilitated research on the dental condition of the Korean people, and ran independent dental clinic. W.J. Schiefley criticised the profit-seeking attitude of Japanese dentists and denturist(=“IPCHISA”, in Korean pronunciation) and emphasized on the significance of Oral Health. He did all kind of dental treatments with advanced equipments like X-ray machine, and managed the collective oral health care for missionaries overseas. He trained medical students and assistants of the dentists with the goal of producing Korean dentists, but he failed due to the Dentist law introduced by Japanese colonial administration that interfered with producing Korean dentists. However, O.R. Avison’s proposal of the establishment of dental schools stimulated the establishment of Kyungsung dental school, which provided the basis for the Dental department in the Severance Union Medical College becoming special training institution for Korean Dentists.
특집논문 3 : 치과대학 치과의학사 교육의 현황과 과제
이주연 ( Jue Yeon Lee ) 연세대학교 의과대학 의사학과 2010 연세의사학 Vol.13 No.2
The first dental history class took place in 1949 at the College of Dentistry, Seoul National University. By the mid-2000s, eight of the eleven dentistry schools in Korea taught dental history. However, dental history education was dropped from curricula following the requirement by the Korean Institute of Dental Education and Evaluation (http://www.kidee.org) that the course “Recognition Standards for Colleges or Graduate Schools of Dentistry” include dental humanities and social science education and that the subject inculcate communication skills, morality, professionalism, and critical thinking. Currently, eight schools (72%) run classes related to dental history; these are worth on average 0.88 units. Of these schools, only three retain the name “dental history” for such classes; two schools use dental humanities and social science-related names. One school has included dental history in its “Introduction to Dentistry” and “Society and Dentistry” courses, and two other schools have included the topic in “Social Responsibility of Dentists” and “Ethics and Oral Hygienists,” respectively. The classes continue to include more modern than diachronic dental history. The study of dental history seeks to produce dentists who possess a general understanding of humanity as social beings, their illnesses, and the nature of and changes in medical treatment. It also aims to produce dentists capable of reacting creatively to societal changes. To achieve these objectives and to ensure that the management and evaluation of the field are more closely connected and perfected, the term “dental history” must be reinstated. In addition, there is a need for more systematic education and research on modern Korean dental history that is related to dentists’ professionalism. The revival of dental history education should yield a problem-based learning approach to writing and discussion that can eventually aid the search for historical evidence-based solutions to the many difficulties that Korean society and medical treatment face.
Enhanced bone formation by transforming growth factor-β1-releasing collagen/chitosan microgranules
Lee, Jue-Yeon,Kim, Kyoung-Hwa,Shin, Seung-Yoon,Rhyu, In-Chul,Lee, Yong-Moo,Park, Yoon-Jeong,Chung, Chong-Pyoung,Lee, Seung-Jin 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.16
Collagen/chitosan composite microgranules were fabricated as bone substitutes for the purpose of obtaining high bone-forming efficacy. The microgranules have the flexibility to fill various types of defect sites with closer packing. The interconnected pores formed spaces between the microgranules, which allowed new bone ingrowth and vascularization. In addition, the transforming growth factor-beta 1 (TCF-βl) was incorporated into the microsranules in order to improve bone-healing efficacy. The collagen/chitosan microgranules were fabricated by dropping a mixedsolution into a NaOH/ethanol solucon. TCF-β1 was loaded into the collagen/chitosan microgranules by soaking the microgranules in a TCF-βl solution. Scanning electron microscopy (SEM) observations and experiments examining the release of TCF-β1 from chitosan and the collagen/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules and cell proliferation was evaluated using a dimethylthiazole tetrazolium bromide assay. The differentiated cell function was assessed by measuring the alkaline phosphatase (ALPase)activity as well as detecting an osteocalcin assay. The in vivo bone-regeneration experiments were performed using a rabbit calvarial defect model. TCF-β1 was released from the collagen/chitosan microgranules at a therapeutir concentration for 4 weeks. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer manner. The proliferation of the osteoblasts on the TCF-β1-loaded microgranules was the high-est among the different types of microgranules tested. The ALPase activity and osteocalcin level of ail the samples increased during the culture period, and the TCF-β1-loaded microgranules had a significantly higher ALPase activity and osteocalcin content than the other microgranules. The TCF-β1-loaded microgranules demonstrated a higher bone-regenerative capacity in the rabbit calvarial defects after 4weeks than the TCF-01-unloaded microgranules.