A Novel Flexible Color Display Using Two-Step UV Exposure Method

Jin, Yan,Hong, Zhe,Jeon, Chang Woo,Lee, Burm Young,Kim, Kyoung-Sun,Kwon, Soon-Bum Informa UK (TaylorFrancis) 2014 Molecular Crystals and Liquid Crystals Vol.595 No.1

Cross-reactivity of Toxocariasis with Crude Antigen of Toxascaris leonina Larvae by ELISA

JIN YAN,Chenghua Shen,허선,최민호,홍성태 대한의학회 2015 Journal of Korean medical science Vol.30 No.5

Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world. Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers. Recently, patients of clinically diagnosed toxocariasis are increasing and require correct diagnosis in Korea. The present study investigated serological cross-reactivity between crude antigens of T. canis (TCLA) and T. leonina (TLLA) larvae. We collected serum specimens from 177 toxocariasis patients who were clinically suspected in the Seoul National University Hospital and 115 healthy controls. An ELISA method for toxocariasis was used to evaluate diagnostic efficacy of TLLA for serodiagnosis of human toxocariasis. The IgG ELISA using TLLA gave 14 (14.3%) positives of 98 TCLA positive specimens among 177 suspected toxocariasis patients. Most of them showed high absorbances with TCLA. In conclusion, there is a partial cross reaction between serum specimens of toxocariasis and TLLA.

MET Gene Copy Number Gain is an Independent Poor Prognostic Marker in Korean Stage I Lung Adenocarcinomas

Jin, Yan,Sun, Ping-Li,Kim, Hyojin,Seo, An Na,Jheon, Sanghoon,Lee, Choon-Taek,Chung, Jin-Haeng Springer - Society of Surgical Oncology 2014 Annals of Surgical Oncology Vol.21 No.2

Continuous Production of Immunoliposomes using a Microvalve-controlled Microfluidic Device (μFD)

Jin, Yan,Kim, So Hyun,Kim, Myunghee,Park, Sungsu Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.10

Immunoliposomes (antibody-conjugated liposomes) are highly useful as both a drug carrier in drug delivery and as a reporting probe in immunodiagnostics. However, antibody conjugation is lengthy and cumbersome, because this includes several steps such as derivatization of the antibody, conjugation of the derivatized antibody to liposomes, and separation of the unbound antibodies from immunoliposomes. Recently, liposome preparation steps have simplified by using microfluidic devices (${\mu}FDs$) where liposomes are formed when a stream of lipids in solvent is hydrodynamically focused between two oblique buffer streams in a microchannel. Herein, we report a simple method for the production of immunoliposomes (rabbit IgG-conjugated liposomes) using microvalve-controlled ${\mu}FD$. The presence of antibody on the liposome was verified by observing the binding of immunoliposomes to rabbit IgG on the surface. The results suggest that immunoliposomes can be easily prepared through sequential mixing of antibody, conjugation reagents, preformed liposomes using microvalve-controlled ${\mu}FD$.

Flavobacterium panaciterrae sp. nov., a β-glucosidase producing bacterium with ginsenoside-converting activity isolated from the soil of a ginseng field.

Jin, Yan,Kim, Yeon-Ju,Hoang, Van-An,Young Jung, Sun,Nguyen, Ngoc-Lan,Woo Min, Jin,Wang, Chao,Yang, Deok-Chun Institute of Applied Microbiology, University of T 2014 The Journal of general and applied microbiology Vol.60 No.2

<P>The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY69(T) is JX233806. A Gram-reaction-negative, oxidase- and catalase-positive, non-gliding motile strain, designated strain DCY69(T), was isolated from the soil of a ginseng field in the Republic of Korea. Colonies of strain DCY69(T) were circular, 0.5-1.5 mm diameter, yellow, and convex on an R2A agar plate after 2 days. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DCY69(T) belonged to the genus Flavobacterium with 90.5-98.3% gene sequence similarity. The major predominant quinone was MK-6. The major cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH and summed feature 3 (containing C16:1ω7c and/or C16:1ω6c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and unidentified polar lipids (L1, L2). The genomic DNA G+C content of strain DCY69(T) was 35.0mol%. The strain DCY69(T) transformed ginsenoside Rb1 into Rd and F2. Based on the polyphasic taxonomic data, strain DCY69(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium panaciterrae sp. nov. is proposed. The type strain is DCY69(T)(= KCTC 32392(T) = JCM 19161(T)), isolated from the soil of a ginseng field in the Republic of Korea.</P>

Regulation of anti-inflammatory cytokines IL-10 and TGF-b in mouse dendritic cells through treatment with Clonorchis sinensis crude antigen

JIN YAN,위혜주,최민호,홍성태,배영미 생화학분자생물학회 2014 Experimental and molecular medicine Vol.46 No.-

Dendritic cells (DCs), which are regarded as the most potent antigen-presenting cells, are involved in innate and adaptiveimmunity. Upon uptake of pathogens, DCs express cell surface markers and secrete cytokines. In this study, we analyzedproduction of cytokines and found that interleukin (IL)-10 and transforming growth factor (TGF)-b production significantlyincreased in bone marrow-derived DCs and a mouse DC line, DC2.4, after treatment with crude antigen (CA) from liver fluke,Clonorchis sinensis. However, expression patterns of several activation molecules did not change. In addition, followingtreatment of DC2.4 cells with antigen from the lung fluke, Paragonimus westermani, production of IL-10 and TGF-bsignificantly increased compared with groups treated with other parasite antigens, Spirometra erinacei plerocercoid CA andEchinococcus granulosus hydatid cystic fluid. We also found that treatment of DC2.4 cells with C. sinensis CA resulted in rapidand significant phosphorylation of extracellular signal-regulated kinase 1/2, a mitogen-activated protein kinase. Followingtreatment of DC2.4 cells with C. sinensis CA, treatment with an inhibitor specific to an extracellular signal-regulated kinaseinhibited production of IL-10 and TGF-b. Our results suggest that CA from C. sinensis has a role in the anti-inflammatoryfunction of DC cells by inducing IL-10 and TGF-b through activation of extracellular signal-regulated kinase 1/2.

ROS1 gene rearrangement and copy number gain in non-small cell lung cancer.

Jin, Yan,Sun, Ping-Li,Kim, Hyojin,Park, Eunhyang,Shim, Hyo Sup,Jheon, Sanghoon,Kim, Kwhanmien,Lee, Choon-Taek,Chung, Jin-Haeng Springer International 2015 Virchows Archiv Vol.466 No.1

<P>ROS1 has attracted much attention as a possible oncogenic driver and ROS1-rearranged tumors show sensitivity to most ALK inhibitors. We aimed to clarify the prevalence of ROS1 gene rearrangement and investigate the clinical implications of ROS1 gene copy number gain (CNG) in non-small cell lung cancer (NSCLC) patients. We carried out fluorescent in situ hybridization with ROS1 and centromere enumeration 6 probes and immunohistochemistry for ROS1 protein expression. ROS1 rearrangement was detected in 3 of 375 samples (0.8 %); all of whom were female, never-smokers, and harbored an adenocarcinoma component. ROS1 gene CNG was found in 18 cases (4.8 %). ROS1 gene CNG was significantly associated with shorter disease-free survival (DFS, 12 vs. 58 months; p = 0.003) and shorter overall survival (OS, 40 vs. 67 months; p <0.001) than the group without CNG. Multivariate analysis confirmed that ROS1 gene CNG was significantly associated with poorer DFS (hazard ratio [HR]=2.16, 95 % confidence interval [CI] = 1.22-3.81, p = 0.008), and OS ([HR] = 2.53, 95 % [CI] = 1.31-4.89, p = 0.006). ROS1 protein overexpression was observed in 5.0 % (18 out of 357), of which 2 cases harbored ROS1 gene rearrangement. There was no statistically significant correlation between ROS1 gene CNG and protein overexpression. This study demonstrated ROS1 gene rearrangement was detected in 0.8 % of surgically resected NSCLC; and ROS1 gene CNG is an independent poor prognostic factor. This survival analyses may contribute to future studies on the utility of ROS1-targeted therapy for patients.</P>

Significance of Serology by Multi-Antigen ELISA for Tissue Helminthiases in Korea

JIN YAN,김은민,최민호,오명돈,홍성태 대한의학회 2017 Journal of Korean medical science Vol.32 No.7

It is clinically important to differentiate tissue-invading helminthiasis. The purpose of this study was to assess the specific immunoglobulin G (IgG) antibody positive rates for clonorchiasis, paragonimiasis, cysticercosis, and sparganosis 4 helminthiases from 1996 to 2006 using multi-antigen enzyme-linked immunosorbent assay (ELISA) in Korea. Results of 6,017 samples, which were referred to our institute for serodiagnosis, were analyzed. The subjects with positive serum IgG antibodies were 1,502 (25.0%) for any of the 4 helminthiases. The overall positive numbers for clonorchiasis, paragonimiasis, cysticercosis, and sparganosis were 728 (12.1%), 166 (2.8%), 729 (12.1%), and 263 (4.4%), respectively. The positive serologic reaction to multi-antigens was determined in 309 (20.6%) of the 1,502 total seropositive subjects. Those with multi-antigen positivity were regarded as positive for the antigen of strongest reaction but cross-reaction to others with weak positive reaction. Annual seropositive rates for those 4 tissue helminthiases ranged from 12.1% to 35.7%. The highest rate was observed in age from 60 to 69 years old and prevalence of men (27.4%; 1,030/3,763) was significantly higher than of women (19.1%; 332/1,741). Hospital records of 165 ELISA positive patients were reviewed to confirm correlation with their clinical diagnosis. Paragonimiasis was highly correlated as 81.8% (9/11), cysticercosis 29.9% (20/67), clonorchiasis 29.0% (20/69), and sparganosis 11.1% (2/18). In conclusion, the multi-antigen ELISA using 4 helminth antigens is useful to differentiate suspected tissue-invading helminthiases, especially ELISA diagnosis of paragonimiasis is reliable. The seropositivity is still high among suspected patients in Korea.

Parasitological Observation in Schoolchildren with Urogenital Schistosomiasis Following Treatment with Three Different Brands of Praziquantel

JIN YAN,차승만,Lee Jinmoo,Elhag Mousab Siddig,홍성태,이영하 대한의학회 2020 Journal of Korean medical science Vol.35 No.46

This study compared the anthelminthic effects of three different brands of praziquantel being used in Sudan against Schistosoma haematobium (S. haematobium) infection. We enrolled 1,286 schoolchildren from six primary schools and examined their urine samples for eggs of S. haematobium at the baseline survey and follow-up two weeks after administering the medication. The schoolchildren were divided into three groups based on the three brands of praziquantel (different material production), with two school children for one brand. The overall baseline prevalence of S. haematobium infection was 15.5%. Two weeks after treatment with brands A, B, and C of praziquantel, cure rates were 87.1%, 82.4% and 83.8% respectively, and the egg-reduction rates were 69.0%, 81.0% and 70.6% respectively. There was no statistically significant difference in cure rates and egg-reduction rates between the three brands. We conclude that the three different commercial brands of praziquantel used in Sudan have similar anthelminthic effects on S. haematobium.

College English Teaching and Testing in China : Achievements and Problems in the Past Two Decades

Jin Yan 서울대학교 2006 외국어교육연구 Vol.9 No.-

In the paper, the development of college English teaching and testing in China in the past two decades will be reviewed focusing on the promulgation and implementation of the National College English Teaching Syllabuses since the 1980s and the launch of the National College English Test (CET) in the late 1980s. The second part of the paper focuses on the achievements made and problems encountered in the development of college English teaching and testing. The English proficiency of college students in China will be presented by referring to the CET Syllabus. Examples of students’ performance in the CET will be given to illustrate their communicative competence. The imbalanced development of the English proficiency of college students across the country will also be discussed and possible explanations given for the discrepancy. The paper will then move on to a discussion on the impact of language testing on teaching with special reference to the washback of the CET on college English teaching in China. Both beneficial and detrimental effects of the CET on teaching and learning will be presented and the social consequences of the large-scale standardized test will be discussed in some detail. The paper ends with a brief introduction to the recent reform of the college English teaching and testing in China.