MicroRNA 452 regulates ASB8, NOL8, and CDR2 expression in colorectal cancer cells

Ji‑Su Mo,Soo‑Cheon Chae 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background MicroRNAs play important roles in the pathogenesis of human diseases by regulating target gene expression in specifc cells or tissues. Previously, we identifed microRNA 452 (MIR452), which was specifcally up-regulated in early stage human colorectal cancer (CRC) tissue. Objective The current study aims to identify and verify the target genes of MIR452 associated with CRC. Methods A luciferase reporter system was used to confrm the efect of MIR452 on ASB8, NOL8, and CDR2 expression. The expression levels of MIR452 and the target genes were evaluated by quantitative RT-PCR (qRT-PCR) and western blotting. Results We verifed the association between MIR452 and three genes, ASB8, NOL8, and CDR2, and showed that their transcripts were down-regulated by MIR452. Up-regulated MIR452 also down-regulated ASB8, NOL8, and CDR2 mRNA and protein levels in CRC cells. CDR2 protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. Conclusions These results suggest that ASB8, NOL8, and CDR2 were target genes of MIR452 in CRC cells and that upregulated MIR452 in CRC tissue regulated ASB8, NOL8, and CDR2 expression during colorectal carcinogenesis.

Newcastle Disease Virus의 Fusion 단백 유전자의 제한효소분절형태(RELP)의 특이성

장경수,김지영,김석,김태용,송영환,허지연,김현수,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

To evaluate the specificity of restriction fragment length polymorphism (RFLP) of Newcastle disease virus (NDV) fusion (F) protein gene for various NDV strains. F protein genes of NDV CBP-1 isolate, LaSota, B1 and Kyojungwon (KJW) were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and RFLP were examined. In RT-PCR using F primer sets for 1.7 kb F gene, the amplified products were observed in CBP-1, LaSota and B1 except KJW. However, F2 primer set for 521 bp yielded the amplified bands with all strains. The amplified F genes (1.7 kbp) were treatd with 15 different restriction enzymes to analyze RFLP and the cleavage patterns. CBP-1 showed various cleavage patterns with 8 enzymes, LaSota, with 11 enzymes and B1, with 11 enzymes. According to the cleavage patterns, the physical maps were constructed, and it was found that CBP-1, LaSota and B1 strains had specific cleavage sites with HinfI, XbaI and XhoI. These findings suggest that RFLP are worth applying in the diagnosis and molecular epidemiological studies of Newcastle disease.

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

하수슬러지 발효고화에 의한 Biosoil의 매립장 복토재 Lysimeter 평가

지민구,김규하,이수구 서울産業大學校 2005 논문집 Vol.54 No.3

현재 하수슬러지의 발생양은 매년 증가하는 추세로 함수율이 높은 유기성 하수 슬러지는 2차 오염을 유발시키고 처리, 처분 단계에서 여러 가지 방법이 논의되고 있지만, 합리적인 처리가 어려워 세계적으로 중요한 과제로 떠오르고 있다. 이에 하수슬러지의 최종처분 및 재활용 방안 마련을 위하여, 하수슬러지를 발효·고화시킨 Biosoil을 매립지의 복토재로 사용하기 위해 현재 매립장에서 복토재로 일반적올 사용되고 있는 토양을 비교, 검토하고자 하였다. 하수 슬러지를 고화, 발효시킨 Biosoil과 일반 복토재로 사용하고 있는 흙을 Lysimeter를 통해 비교 연구하여 복토재로서의 재활용 가능성을 평가해 보고자 하였다. 이에 본 연구에서는 실험 재료의 물리, 화학적 성질을 알아보기 위해 매립전후 일반 토양과 Biosoil의 유기물량, pH. 등을 측정하였으며, 동일한 Lysimeter 2기에 각각 일반토양과 Biosoil을 복토하여 비교실험을 하였다. 장치 내부에는 매립장과 동일하게 생활쓰레기를 성상 분석하여 충진 하였고, 인공강우를 주기적으로 살수하였다. 연구 결과 매립전후 일반토양의 유기물량은 일반흙과 비슷하였으나 Biosoil은 매립 후 유기물량은 다소 낮아졌으며 pH는 매립후 일반토양, Biosoil 모두 낮아졌다. 또한 토질시험결과 Biosoil의 투수게수는 5.68 x 10^(-6)cm/sec로 매립장 복토재로서의 조건을 충분히 만족하는 것으로 나타났다. 실제로 복토후의 모습을 관찰하기 위해 실외에서 실시한 기상실험에서도 건조한 하수슬러지에 비해 재슬러지화가 일어나지 않았으며, 일반흙과 비슷하게 다져지게 나타났다. 위와 같이 하수슬러지를 재활용한 복토재로의 가능성을 살펴본 결과 복토재로서의 사용가능성을 보이고 있으며, 더 많은 연구를 통해 재활용 복토재로의 사용가능성을 더높여야 할 것으로 판단된다.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

기술수준에 따른 여자축구 인프론트 킥 동작의 협응구조

지보경,육동원,김지수,신정택 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate coordination structure of infront kick in women soccer as a function of skill level. To succeed this purpose, four girls' high school students who had never experienced soccer before were selected as novices, and four girls' high school students who had played soccer for over three years as experts in this lab. To recognize the change of coordination structure according to practice, novices had practiced infront kick in soccer for two weeks. consequently they were taken films of kicking motion three times -before practice, after one week practice, after two weeks practice. However, experts were taken once. The experimental instruments for this study consisted of a video camera, reference frame. LED and APAS for digitizing. Angle to angle diagrams and phase plan portraits for qualitative analysis of coordination structure were gotten with LabVIEW 6i. The conclusions are as follow: firstly, novices' coordination structure had been changed since practice. Secondly, there were significantly different between coordination structures of experts and pre-practice novices. Lastly, there were similar between coordination structures of experts and post practice novices.

후두부 발작성 간질파와 동반된 양성 소아기 간질의 임상적 연구

유영수,이상도,김지언,임정근,박영춘 대한신경과학회 1996 대한신경과학회지 Vol.14 No.4

소아에서 Fogarty 카테터를 이용한 일측폐 환기 마취 경험

유수봉,강효석,임지훈,김두식,류시정 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Thoracic surgery may be greatly facilitated by causing selective atelectasis of the lung being operated on (one-lung ventilation anesthesia). But the tube used for one-lung ventilation is greater outer diameter than conventional endotracheal tube when compare to each other with same inner diameter, so in some young children it is impossible to one-lung ventilation using double lumen tube or bronchial blocker tube because of narrow vocal cord. We performed one lung ventilation anesthesia using conventional endotracheal tube and Fogarty catheter as a bronchial blocker in 11-year old women who received lung biopsy surgery and left lower lobectomy.

다 파장 광원 개발을 위한 온도 안정화 특성에 관한 연구

지유강,박수봉 동신대학교 2006 論文集 Vol.16 No.-

본 논문은 광 전송 시스템에서 정보를 전송하기 위하여 필요한 안정된 광원을 얻기 위한 것으로써, 광원을 얻기 위한 광원시스템은 광원 모듈부와 디지털 제어부 그리고 온도 안정화부로 구성하였다. 본 논문에서는 레이져 다이오드의 광 출력을 안정화 시키기 위하여 온도센서와 자체개발한 TEC(Thermo Electric Cooler)를 이용하여 온도제어회로를 구성함으로써 안정된 광원을 얻을 수 있었다. 제안된 온도 안정화부의 온도범위는 ±0.5도 이내에서 제어가 가능하며 이와 같이 구성된 광원시스템의 4파장 광원은 ±0.05dB 이내에서 안정화되었다. In this paper, we composed optical source system of optical module section, digital control section, and temperature stability section to acquire optical source and to transmit information in optical transmit system, we could obtaion optical source stabilized as composed of temperature control circuit to take advantage of temperature sensor and ourselves developed TEC (Thermo Electric Cooler) to balance optical output power of laser diode. The temperature range of proposed temperature stability section make control possible within ±0.5 degree, also four wavelength optical source stabilized less than ±0.05dB.

대칭형 복사기 줌 렌즈계의 광학적 성능 분석

지택상 ; 박수봉 ; 김봉환 김천대학교 2001 김천대학교 논문집 Vol.22 No.-

We analyzed to calculate the optical capacity, after we chose the designed symmetric 3 group zoom copier lens system in this paper.