종합효소연쇄반응의 적정화

장숙진 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

Abstract : Polymerase chain reaction (PCR) is the one of the most widely used molecular biological techniques because of it's simplicity, flexibility, sensitivity, and robustness. Although the principle of the PCR is conceptually simple, many attempts to perform PCR fail to yield meaningful results, In those cases of PCR failure, performing control reactions will establish whether or not the PCR conditions are functioning appropriately. PCR optimization and other efforts to seek the causes of the PCR problems and adequate treatments for them will lead to PCR success. Optimization of PCR conditions includes design of nice primers, and fine tuning of the concentrations of MgCl₂, annealing temperature, pH of the reaction, dNTP, and Taq polymerase. For troubleshooting of PCR, some commonly observed problems in the period of setting up PCRs for the first time or in amplification reactions were described. Also the explanation about the possible causes and suggested remedies for them were provided, In addition, methods for improving PCR such as hot start PCR, nested PCR, touch down PCR, and booster PCR were introduced.

Dot-ELISA를 이용한 Chlamydia trachomatis 감염의 혈청학적 진단

장숙진,김덕언,김홍석,최태열 대한임상병리학회 1992 대한임상병리학회지 Vol.12 No.2

간호학생의 임상실습 만족도에 관한 조사 연구 : With special Reference to Soon Cheon Nursing Junior colleage.

장숙희 순천청암대학 1986 論文集-順川看護專門大學 Vol.11 No.-

As it prouides nusing students the opportunity for correlating principle and practice, has been Considered as one of the most important part of fusing education. The purpose of this study is to suggest the improved principles of guidance and curriculum for rousing education by surveying veconigations and attitudes of students of nursing school. This study was performed from November 21 to December 9, and students were given questimnaire. First answer is 143 student, and second is 147 student, and Total answer is 190 student, but first answer and second answer is same person.(150 zinior) Data analysis included percentage, Mean and standard error, p-Value. pearson Correlation Correlation coefficient, t-test, and AZUKCY through computer stratistic disposition. Results are as for lows ; 1. Level of Satisfaction of contents ( mean Score = 2.5 ) revealed to be Low. 2. Level of Satisfaction of environment ( mean Score = 3.21 ) revealed to be high. 3. Level of Satisfaction on hours ( mean Score = 2.77 ), guidance ( mean Score = 2.93). 4. Level of Satisfaction of evaluation ( mean Score = 2.87 ). 5. Level of Satisfaction of relation ship ( mean Score = 2.63 ). 6. Level of Satisfaction revealed to be Low ( total mean Satisfa ction Score = 2.88).

컴퓨터 하드웨어 학습을 위한 CAI 도구 설계

장숙현,임인택 釜山外國語大學校 敎育大學院 2005 敎育論叢 Vol.6 No.-

In this paper, CAI program using Macromedia's Director MX was developed. The analyzed educational processing and dynamic method are added to this software to accomplish the object of teaching for 5th grade of elementary. Through the development of CAI program, several results are accomplished as follows. At first, the CAI program should gain the interest from students and it should answer to various questions so that students can study for themselves. At second, before developing the CAI program, sufficient pre-researches are needed about choosing the suitable contents, object of the study, and the right time to use the CAI program.

Ribbon형 Co1-x Crx (x=0.27, 0.29, 0.30, 0.32, 0.35) 합금의 자기특성

장현숙,김미양,이장로,이용호 숙명여자대학교 자연과학연구소 1994 자연과학논문집 Vol.- No.5

급냉법으로 리본형 결정질 Co1-x Crx (x=0.27, 0.29, 0.30, 0.32, 0.35)합금을 제조하여 Cr의 조성에 따른 자기적 특성을 조사하였다. 포화자화값은 벌크값보다 컸지만 박막의 경우보다는 작았다. Cr의 함량이 증가함에 따라 포화자화값은 감소하였다. 27 at.% Cr의 경우를 예외로 하면 Cr 함량 증가에 따라 결정립의 크기는 감소하였고, 항자력은 증가하였다. 리본형 Co1-x Crx 는 포화자화값의 온도 의존성이 비정질 시료인 경우와 유사한 경향을 나타내었으며 Cr의 함량이 증가할수록 Curie온도(Tr)는 감소하였다. Cr 함량의 증가에 따라 exchange interaction의 평균자승거리<r²>와 spin wave stiffness 상수D는 감소하는 경향을 나타내었다. 천이금속(Co)의 원자당평균 자기모멘트는 Cr의 증가에 따라 선형적으로 감소하였다. The magnetic cnaracteristics of crystalline Co1-x Crx (x=0.27, 0.29, 0.30, 0.32, 0.35) ribbons prepared by the rapid solidification were investigated. The saturation magnetization for ribbon was greater than that for bulk alloys containg the same amount of Cr. but smaller for the films. The grain size of ribbon became smaller and coercive force increased with increasing Cr contents except case of 27 at.% Cr. The saturation magnetization decreased with increasing Cr contensts. The temperature dependence of saturation magnetization for Co1-x Crx ribbon showed the similar behavior as the amorphous alloy and the Curie temperatures were decreased with the increase of Cr contents. The spin wave stiffness constant and the mean square range of exchange interaction, also were decreased. The average magnetic moment per transition metal atom was linearly decreased with increasing the contents of Cr.

미생물의 역학적 분석을 위한 검사 과정의기본 개념

장숙진 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

노인의 건강 상태, 사회적 지지와 생활만족도와의 관계

장숙희 노인간호학회 2006 노인간호학회지 Vol.8 No.1

This purpose of this study was to examine the relationship between health status, social support and life satisfaction in the elderly. Data were collected from 82 elderly over 65, from October 1, 2005 to October 30, 2005 in G city. The instrument used were health status scale developed by Young-Hee Choi et al. (1990), social support scale developed by Jee-Won Park (1985) and partially amended by Joo-Woo Hong (1999), and life satisfaction scale developed by Jin Yoon (1982). Data were analysed frequency, percentage, mean, standard deviation, t-test, ANOVA and Person’s correlation coefficient Using SPSS/PC (12.0) program. The results were as follows : 1. In health status of subject, The mean score for physical health status was 2.6± 23, psychological health status was 2.4± 39. The mean score for social support was 3.4± 88. The mean score for life satisfaction was 2.0± 52. 2. There were significant differences in health status according to general characteristics such as education level, perceived health status, share of living expense and illness existence. 3. There were significant differences in social support according to general characteristics such as perceived health status and pocket money. 4. There were significant differences in life satisfaction according to general characteristics such as living together status, perceived health status, illness existence and pocket money. 5. There were significant positively correlated physical health status and psychological health status (r=.662, p=.000), physical health status and social support (r=.303, p=.006), physical health status and life satisfaction (r= .514, p=.000), psychological health status and social support (r=.489, p=.000), psychological health status and life satisfaction (r=.818, p= .000), social support and life satisfaction (r= .375, p=.001). From above result, psychological health status of the elderly themselves are found as important variables to affect life satisfaction Thus, psychological health status should be considered in order to improve nursing intervention for life satisfaction of the elderly.

셰익스피어의 희곡 "Hamlet"의 무대의상 디자인

장무경,박숙현,이순덕 慶星大學校 1999 論文集 Vol.20 No.2

The purposes of this study are 1) to examine the characteristics and types of costume which had a vogue in the Shakespeare's times, 2) to analyze characters' psychological aspect and change it to visual image and then apply to design, 3) to design new stage costume through the symbolism of the colors and the simplication of the costume added modern sense on the basis of the costume in the early Renaissance in Shakespeare's Drama "Hamlet". The results of study are as follows. Hamlet's costume is composed of velvet jacket transformed Houpplande for black mourning clothes and black tight pants. Gertrude's costume is composed of red-purple gown with hanging- sleeves and gray silk underdress. Ophelia's costume is composed of white chemise and green overdress.

임상 증상이 심하지 않은 심낭 삼출 환자의 장기 추적 결과

배장호,김기식,강미숙,남명희,김미정,김민수,김기영,님창욱,김윤년,김권배,정시전,김여희 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.7

간질발작후 MRI 영상에서 뇌 종창을 보인 3예 보고

홍현숙,김대호,이혜경,박재성,박성진,조준희,차장규,백상현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

We report seizure induced brain swelling in three patients. MRI revealed a cortical, subcortical(3/3), and basal ganglia(2/3) high signal intensity on T2- weighted and FLAIR images. The lesion shows low signal on T1-weighted image, and enhanced after contrast administration. Follow up study after 27 days in one case revealed complete resolution of the lesion. The reversible MRI findings may be the results of a local brain swelling, breakdown of blood-brain permeability, and a defect of cerebral autoregulation during seizure activity. The transient nature of such findings have to be considered in the differential diagnosis such as reversible ischemia, migraine, inflammation and infection.