http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
후오밍쿤(Huo Ming-Kun) 경북대학교 인문학술원 2009 동서사상 Vol.6 No.-
당대 중말기 도교의 전환 시기에 열광적으로 단을 수련하던 기술적 고리가 실패로 끝나자, 심성을 중시하는 성선(成仙)이론이 성립하였다. 이 심성수선관(心性修仙觀)은 당송의 사이(唐宋之際)에 도교가 외단(外丹)으로부터 내단(內丹) 및 신선사상(神仙思想)으로 넘어가는 변화과정 중의 중요한 고리이다. 이것은 도교 전환시기의 이론 가운데 중요한 위치를 차지할 뿐만이 아니라 당대 중말기 이후의 종교, 철학, 문학에 대하여 심원한 영향을 미쳤다.
?明琨(Huo Ming-Kun) 경북대학교 인문학술원 2009 동서사상 Vol.6 No.-
During the transformations in Daoism occurring in the Mid and Late Tang period, the obsession over techniques for the concoction of medicines and elixirs towards the achievement of immortality had ended in failure, and attention became focused on the formation of theories involving the cultivation of inner nature and temperament’s role in this quest. This concept of inner nature leading to immortality constituted a key phase in the Tang Song era process whereby external techniques purportedly offering immortal status gave way to internal methods, standing not only in a crucial position within the theoretical background during this phase of transition within Daoism, but exerting a deep influence on religion, philosophy and literature in general from the Mid Late Tang era forward.
m6A writer gene fl(2)d regulates the metamorphosis of silkworm Bombyx mori
Huang Qunxia,Huo Chunyue,Qian Yuxin,Zhao Chenyue,Cai Zhiyan,Zhang Kun,Zhao Xuhui,Guo Shigang,Kan Yunchao,Li Dandan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
The N6-methyladenosine (m6A) is one of the most abundant posttranscriptional modifications on RNAs, which is mediated by its effector proteins—writers, readers, and erasers. Female-Lethal(2)D (Fl(2)d) is one of the key writers to stabilize the interaction between another two writers Methyltransferase-like 3 (METTL3) and Methyltransferase-like 14 (METTL14). Here with dsRNA transfection, fl(2)d was knocked down in the BmN4 cell lines of silkworm, the expression of 61 genes were significantly increased, and 86 genes were significantly decreased after fl(2)d repression, which encoded proteins related to catalytic, hydrolase activity or in metabolism of nucleic acid. To elucidate the functional role of fl(2)d during silkworm development, dsRNA of which was injected into the hemolymph of the 2-d-old fourth instar larvae, the mounting process of silkworm was delayed, the expression level of genes in metabolism, RNA processing and ecdysone signaling pathway decreased significantly after fl(2)d knockdown, such as genes encoded Ubiquitin protein ligase E3C, the nuclear pore complex protein Nup98-Nup96, and the m6A reader YTHDF3, as well as Broad Complex isoform 2 (BRC-Z2), Fushi Tarazu Factor-1 (FTZ-F1), lactate dehydrogenase (LDH) and the ecdysone-induced protein 74EF isoform A (E74A). Total m6A level of RNAs also decreased after fl(2)d repression. These indicated that decreased level of fl (2)d and YTHDF3 decay the m6A level and translation of their target genes, which might lead to the retardation of metamorphosis, and resulted in the delayed mounting of silkworm.
Wang, Li-Meng,Xie, Kun-Peng,Huo, Hong-Nan,Shang, Fei,Zou, Wei,Xie, Ming-Jie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
The growth of many breast tumors is stimulated by IGF-1, which activates signal transduction pathways inducing cell proliferation. $ER{\alpha}$ is important in this process. The aim of the study was to investigate relationships in vitro among inhibitory effects of luteolin on the growth of MCF-7 cells, IGF-1 pathway and $ER{\alpha}$. Our results showed that luteolin could effectively block IGF-l-stimulated MCF-7 cell proliferation in a dose- and time-dependent manner and block cell cycle progression and induce apoptosis evidenced by the flow cytometric detection of sub-G1DNA content. Luteolin markedly decreased IGF-l-dependent IGF-IR and Akt phosphorylation without affecting Erk1/2 phosphorylation. Further experiments pointed out that $ER{\alpha}$ was directly involved in IGF-l induced cell growth inhibitory effects of luteolin, which significantly decreased $ER{\alpha}$ expression. Knockdown of $ER{\alpha}$ in MCF-7 cells by an $ER{\alpha}$-specific siRNA decreased the IGF-l induced cell growth inhibitory effects of luteolin. $ER{\alpha}$ is thus a possible target of luteolin. These findings indicate that the inhibitory effect of luteolin on the growth of MCF-7 cells is via inhibiting IGF-l mediated PI3K-Akt pathway dependent of $ER{\alpha}$ expression.
( Yan Yu Zhao ),( Kun Meng ),( Hui Ying Luo ),( Pei Long Yang ),( Peng Jun Shi ),( Huo Qing Huang ),( Ying Guo Bai ),( Bin Yao ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.8
A xylanase gene, xyn7c, was cloned from Paenibacillus sp. 12-11, an alkalophilic strain isolated from the alkaline wastewater sludge of a paper mill, and expressed in Escherichia coli. The full-length gene consists of 1,296 bp and encodes a mature protein of 400 residues (excluding the putative signal peptide) that belongs to the glycoside hydrolase family 10. The optimal pH of the purified recombinant XYN7C was found to be 8.0, and the enzyme had good pH adaptability at 6.5-8.5 and stability over a broad pH range of 5.0-11.0. XYN7C exhibited maximum activity at 55℃ and was thermostable at 50℃ and below. Using wheat arabinoxylan as the substrate, XYN7C had a high specific activity of 1,886 U/mg, and the apparent Km and Vmax values were 1.18 mg/ml and 1,961 ?mol/mg/min, respectively. XYN7C also had substrate specificity towards various xylans, and was highly resistant to neutral proteases. The main hydrolysis products of xylans were xylose and xylobiose. These properties make XYN7C a promising candidate to be used in biobleaching, baking, and cotton scouring processes.
Yin, Xiao-Yan,Zhong, Wu-Kun,Huo, Jiao,Chang, Xu,Yang, Zhong-Hua 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
In this paper, an optimal semi-continuous process for vinegar production from edible alcohol through biotransformation by acetic acid bacteria (AAB) WUST-01 was developed. The optimized medium composition for the starting-up stage was glucose 5.1 g/L, yeast extract 26.2 g/L, and ethanol 11.9 mL/L, and the optimal ethanol for the following semi-continuous stage was 50 mL/L. In the semi-continuous biotransformation process, the optimal withdraw ratio was 50% of working volume with 12 h cycle time. With these conditions, the total acidity could reach to 77.3 g/L and the acidity productivity could reach to 3.0 g/(L h) in a 5 L reactor. Furthermore, it was investigated to strengthen vinegar synthesis through enhancing alcohol dehydrogenase and aldehyde dehydrogenase activity in AAB by ferrous ion and pueraria flower extract as the enzyme regulators. With these regulators, the vinegar synthesis efficiency can be improved 16.3 and 13.2% respectively.
Gao Liai,Zhou Yongjie,Su Kun,Hou Chenwei,Huo Limin 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.10
It is one of the effective methods to improve the reliability of the distribution system by using looped network power supply. With the development of new energy technology, distributed generation has been more widely used. But a small power system named micro-grid which is assembled by the device such as distributed generation, energy storage device and controllable load in a certain operating rules catches the attention of people. It can operate in islanded mode with the power grid failure, which can greatly improve the reliability of the system. Traditional analytical method and Monte Carlo method are difficult to evaluate its reliability accurately. In this paper, a new artificial intelligence method based on Bayesian networks is used for the system reliability analysis and evaluation. The corresponding Bayesian networks are established for the reliability test bus 2 which containing looped network and the improve reliability test bus 6 which containing micro-grids separately. With the help of causal reasoning and diagnostic reasoning, the supply influence of different types of DG output intermittent, operation mode on loads are detected, the key factors which have an impact on important load power supply reliability are found out, which is the theoretical foundation for practical engineering decision, so as to improve system reliability, identify failures and make maintenance scheduling.
Xiao-Yan Yin,Xu Chang,Wu-Kun Zhong,Jiao Huo,Zhong-Hua Yang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
In this paper, an optimal semi-continuous process for vinegar production from edible alcohol through biotransformation by acetic acid bacteria (AAB) WUST-01 was developed. The optimized medium composition for the starting-up stage was glucose 5.1 g/L, yeast extract 26.2 g/L, and ethanol 11.9 mL/L, and the optimal ethanol for the following semi-continuous stage was 50 mL/L. In the semi-continuous biotransformation process, the optimal withdraw ratio was 50% of working volume with 12 h cycle time. With these conditions, the total acidity could reach to 77.3 g/L and the acidity productivity could reach to 3.0 g/(L h) in a 5 L reactor. Furthermore, it was investigated to strengthen vinegar synthesis through enhancing alcohol dehydrogenase and aldehyde dehydrogenase activity in AAB by ferrous ion and pueraria flower extract as the enzyme regulators. With these regulators, the vinegar synthesis efficiency can be improved 16.3 and 13.2% respectively.