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PDEA-coated Magnetic Nanoparticles for Gene Delivery to Hep G2 Cells
Hanwen Sun,Xin-jun Zhu,Lian-ying Zhang,Xiangling Gu,Jinghe Wang,Jing Li,Yancong Zhang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4
Poly(2-(diethylamino)ethyl methacrylate) coated magnetic nanoparticles (PDEA-MNPs) were synthesized as a new gene nanocarrier to delivery plasmids (pEGFPN1and pRL-TK) into human hepatoma (Hep G2) cells. The PDEA-MNPs shows the pH-sensitive property. These nanoparticles are positively charged at acidic pH and negatively charged at neutral or alkaline pH. The PDEAMNPs exhibited a low cytotoxicity in Hep G2 cells. PDEA-MNPs could bind and protect DNA from DNase I degradation. The transfection study demonstrated that the PDEA-MNPs could carry plasmid into Hep G2 cells and exhibited a high gene transfection efficiency. These results indicated that the novel magnetic nanoparticles could enhance gene transfection in vitro and hold the potential to be a promising non-viral nanodevice.
Liquefaction of Granular Material under Multi-directional Cyclic Loading
( Hanwen Zhang ),( Yunming Yang ),( Hai-sui Yu ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
Liquefaction of soil is one of the major contributor of construction damages caused by earthquake, which contains complicated loading directions and magnitudes. To investigate the liquefaction behavior of granular material during complex earthquake loading, a series of strain-controlled multi-directional cyclic simple shear tests are conducted on uniform-sized glass beads. The imposed cyclic loading includes circular and Figure-8 shape paths. Conventional uni-directional cyclic shearing path has also been conducted for comparison. After liquefaction initiates, the samples are reconsolidated and subjected to monotonic simple shearing to investigate their post-cyclic behavior. The test results indicate that samples subjected to multi-directional cyclic shearing are more prone to liquefy comparing with the conventional cyclic path. Moreover, a linear correlation between the cycle number for liquefaction and the cyclic strain amplitude is postulated for soils under various cyclic shearing conditions (e.g., vertical confining stress, relative density, and cyclic shearing path). In the light of these results, the cyclic shear strain amplitude and the cyclic path have significant influences on the specimen cyclic liquefaction resistance and the post-cyclic monotonic shear strength.
Research on Satisfaction Evaluation Based on Tourist Big Data
( Hanwen Guo ),( Ziyang Liu ),( Zeyu Jiao ) 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.1
With the improvement of people's living standards and the development of tourism, tourists have greater freedom in choosing destinations. Therefore, as an indicator of satisfaction with scenic spots, tourist comments are becoming increasingly prominent. This paper aims to compare and analyze the landscape image of the Five Great Mountains in China and provide specific strategies for its development. The online reviews of tourists on the Online Travel Agency (OTA) website about the Five Great Mountains from 2015 to 2018 are collected as research samples. The text analysis method and R language are used to analyze the content of the tourist reviews, while the high-frequency words in the word cloud are used for visual display. In addition, the entropy weight method is used to determine the index weight and tourist satisfaction is evaluated to understand the weaknesses of those scenic spots. The results of the study show that firstly, the tourist satisfaction with the Five Great Mountains is basically consistent with its popularity. Secondly, through weight analysis, tourists pay special attention to the landscape features and environmental health of the scenic area, so that relevant departments should focus on building the landscape characteristics and improving the environmental health of the scenic area. At the same time, the accommodation and service management of the scenic spot cannot be ignored. Finally, according to the analysis results, suggestions are made on how to improve the tourist satisfaction with the Five Great Mountains.
Competition of sorghum cultivars and densities with Japanese millet (Echinochloa esculenta)
Wu, Hanwen,Walker, Steven R.,Osten, Vikki A.,Robinson, Geoff The Korean Society of Weed Science and The Turfgra 2010 Weed Biology and Management Vol.10 No.3
Field studies were conducted at two locations in southern Queensland, Australia during the 2003-2004 and 2004-2005 growing seasons to determine the differential competitiveness of sorghum (Sorghum bicolor L. Moench) cultivars and crop densities against weeds and the sorghum yield loss due to weeds. Weed competition was investigated by growing sorghum in the presence or absence of a model grass weed, Japanese millet (Echinochloa esculenta). The correlation analyses showed that the early growth traits (height, shoot biomass, and daily growth rate of the shoot biomass) of sorghum adversely affected the height, biomass, and seed production of millet, as measured at maturity. "MR Goldrush" and "Bonus MR" were the most competitive cultivars, resulting in reduced weed biomass, weed density, and weed seed production. The density of sorghum also had a significant effect on the crop's ability to compete with millet. When compared to the density of 4.5 plants per $m^2$, sorghum that was planted at 7.5 plants per $m^2$ suppressed the density, biomass, and seed production of millet by 22%, 27% and 38%, respectively. Millet caused a significant yield loss in comparison with the weed-free plots. The combined weed-suppressive effects of the competitive cultivars, such as MR Goldrush, and high crop densities minimized the yield losses from the weeds. These results indicate that sorghum competition against grass weeds can be improved by choosing competitive cultivars and by using a high crop density of >7.5 plants per $m^2$. These non-chemical options should be included in an integrated weed management program for better weed management, particularly where the control options are limited by the evolution of herbicide resistance.