Genetic Studies of Response to Mature Embryo Culture and Relationship with Agro-Morphological Traits and Molecular Markers in Wheat

( Bahaa El-dein El-sayd Abd El-fatah ) 한국육종학회 2018 Plant Breeding and Biotechnology Vol.6 No.3

Five concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and three combinations of indole-3-acetic acid (IAA) and benzyl-amino purine (BA) were used to analyze response to mature-embryo culture for six genotypes of wheat (Triticum aestivum L.) The differences between genotypes as well as between the concentrations of hormones were significant for three tissue culture traits, namely callus formation (C.%), embryogenic calli (E.C.%) and plant regeneration per embryogenic callus (No. Sh/E.C.). Murashige and Skoog basal medium (MS) supplemented with 7 mg/L 2,4-D showed the highest percentage of callus formation while the highest percentage of embryogenic calli (% of E.C.) was obtained on MS medium supplemented with 4 mg/L 2,4-D. MS medium containing 0.1 mg/L IAA and 1 mg/L BA exhibited the highest number of plant regeneration (No. Sh/E.C.). A half diallel mating was designed to produce 15 crosses among six genotypes. Mean square of the diallel analysis of variance indicated highly significant differences among the parents and F1 hybrids for E.C.% and No. Sh/E.C. traits. The results revealed that Lin-6 (P4) was the best combiner for E.C.% and No. Sh/E.C. Estimates of narrow-sense heritability were 83.1% and 69.0% for E.C.% and No. Sh/E.C., respectively. The genetic diversity among six wheat genotypes was analyzed using three tissue culture traits, 10 agro-morphological traits and three molecular marker systems. The Mantel test showed a positive and significant correlation between tissue culture traits studied and each of agro-morphological traits and molecular marker systems.

Effect of Sewage Wastewater Treated by Nostoc muscorum and Anabaena subcylindrica on the Growth of Zea mays and Phaseolus vulgaris

Mohamed El Anwar Hosain Osman,Wagih Abd EL Fatah EI Shouny,Mostafa Mohamed El Sheekh,Eman Wahba El Sayed El Gammal 한국식물학회 2006 Journal of Plant Biology Vol.49 No.5

Synthesis and Characterization of New Transition Metal Complexes of Schiff-base Derived from 2-Aminopyrimidine and 2,4-Dihydroxybenzaldehyde and Its Applications in Corrosion Inhibition

Ouf, Abd El-Fatah M.,Ali, Mayada S.,Soliman, Mamdouh S.,El-Defrawy, Ahmed M.,Mostafa, Sahar I. Korean Chemical Society 2010 대한화학회지 Vol.54 No.4

New complexes cis-[$Mo_2O_5(Hapdhba)_2$], trans-[$UO_2(Hapdhba)_2$], [Pd(Hapdhba)Cl($H_2O$)], [Pd(bpy)(Hapdhba)]Cl, [Ag(bpy)(Hapdhba)], [$Ru(Hapdhba)_2(H_2O)_2$], [$Rh(Hapdhba)_2Cl(H_2O)$] and [Au(Hapdhba)$Cl_2$] are reported, where $H_2$apdhba is the Schiff-base derived from 2-aminopyrimidine and 2,4-dihydroxy benzaldehyde. The complexes were characterized by IR, electronic, $^1H$ NMR and mass spectra, conductivity, magnetic and thermal measurements. The inhibitive effect of $H_2$apdhba for the corrosion of copper in 0.5 M HCl was also determined by potentiodynamic polarization measurements. 새로운 착물인 cis-[$Mo_2O_3(Hapdhba)_2$], trans-[$UO_2(Hapdhba)_2$], [Pd(Hapdhba)Cl($H_2O$)], [Pd(bpy)(Hapdhba)]Cl, [Ag(bpy) (Hapdhba)], [$Ru(Hapdhba)_2(H_2O)_2$], [$Rh(Hapdhba)_2Cl(H_2O)$] 및 [Au(Hapdhba)$Cl_2$]를 보고한다. 여기서 $H_2$apdhba는 2-aminopyrimidine 및 2,4-dihydoxybenzaldehyde에서 비롯된 Schiff-염기이다. 이들 착물은 IR, UV-Vis 그리고 질량 스펙트럼을 비롯하여 전기전도도, 자기 및 열 분석을 통해 특성을 조사하였다. 구리의 부식에 대한 $H_2$apdhba의 방해효과는 0.5 M HCl에서 potiodynamic polarization 측정을 통해 조사하였다.

Biocomponent-based microalgal transformations into biofuels during the pretreatment and fermentation process

Ha, Geon-Soo,El-Dalatony, Marwa M.,Kim, Do-Hyeon,Salama, El-Sayed,Kurade, Mayur B.,Roh, Hyun-Seog,El-Fatah Abomohra, Abd,Jeon, Byong-Hun Elsevier 2020 Bioresource technology Vol.302 No.-

<P><B>Abstract</B></P> <P>Microalgal cell wall integrity and composition have a significant impact on the fermentation process and biofuel recovery. In this study, various biofuels (bioethanol, higher alcohols (C3-C5), and biodiesel) were produced by the fermentation of carbohydrates and proteins, and transesterification of lipids from three different microalgal strains (<I>Pseudochlorella</I> sp., <I>Chlamydomonas mexicana</I>, and <I>Chlamydomonas pitschmannii</I>), each possessing different proportions of bioconstituents (carbohydrates, proteins, and lipids). Changes in the cell wall structure and thickness were observed before and after fermentation using transmission electron microscopy. <I>Pseudochlorella</I> sp. showed the highest yields of bioethanol (0.45 g-ethanol/g-carbohydrates), higher alcohols (0.44 g-higher alcohols/g-proteins), and biodiesel (0.55 g-biodiesel/g-lipids), which consequently revealed a maximum energy recovery (42%) from whole constituents. This study suggests that different physiological properties, including cell wall thickness and the proportion of bioconstituents in microalgae, could have a significant impact on the pretreatment and fermentation efficiencies for biofuels production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Algal biocomponents has an influence on pretreatment and fermentation efficiency. </LI> <LI> Highest biofuels yield (0.44–0.55 g/g) was obtained from <I>Pseudochlorella</I> sp. </LI> <LI> Cell wall thickness dependent on bioconstituents and affected biofuels yield. </LI> <LI> Highest total energy recovery (42%) was achieved using suitable <I>Pseudochlorella</I> sp. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Protective effect of Arthrospira platensis against liver injury induced by copper nanoparticles

Reham Ebaid,Abd El-Fatah Abomohra,Elhussainy Elhussainy,Safinaz El-Shourbagy,Sameh Ali 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.3

Although copper oxide nanoparticles (nano-CuO) are one of widely used engineered nanoparticles, their human and animal exposure led to particular structural changes in tissues and organs. The present study was undertaken to examine the protective effects of the blue-green microalga Arthrospira platensis against nano-CuO-induced hepatotoxicity in albino rats. Results showed that nano-CuO injection led to significant reduction in serum albumin level with a significant increase in alanine transaminases (ALT) and aspartate transaminases (AST) activities after 24 h of administration. In addition, nano-CuO administration resulted in significant increase in malondialdehyde (MDA) level and catalase (CAT) activity, with a significant reduction in reduced glutathione (GSH) level. Administration of A. platensis alone did not significantly alter the normal levels of aminotransferase enzymes, antioxidants or oxidative stress marker. However, pretreatment with A. platensis prior to nano-CuO intoxication attenuated the levels of ALT, AST (liver function markers) and MDA (lipid peroxidation marker). It also normalized the levels of both hepatic enzymatic and non-enzymatic antioxidants (CAT and GSH, respectively). In conclusion, the present study confirmed the hepatotoxicity of nano-CuO at 10 mg kg−1 administration dose. However, administration of A. platensis could represent a significant protective mechanism against hepatotoxicity.

Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba)

Amer F,Mahmoud,Bahaa E,S,Abd El-Fatah 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.1

Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes. The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza-2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible. The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.