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배양 Caco-2 세포 단층막 실험계에서 트란스페린과 옥시다아제효소 포합체의 세포막투과
김동출,김지혜 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-
Transport study of horseradish peroxidase and transferrin-horseradish peroxidase conjugate was performed using an in vivo Caco-2 cell cultured monolayer grown on a polycarbonate membrane of Transwell^ⓡ. Horseradish peroxidase was not transported across Caco-2 cell monolayer. Transferrin-horseradish peroxidase conjugate was transported through Caco-2 monolayer. The apparent membrane permeability coefficient(P_app) of transferrin horseradish peroxidase conjugate was 6.54×10^-7cm/sec in the presence of 50μg/ml brefeldin-A. These results suggest the transferrin receptor mediated transcytosis of transferrin-horseradish peroxidase conjugate across Caco-2 cell monolayer.
Dong Yi Jie,Liu Zhen Hua,Zhou Jian Qiao,Zhan Wei Wei 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.4
Objective: To prospectively evaluate the efficacy of lauromacrogol injection for ablation (LIA) of benign predominantly cystic thyroid nodules and its related factors. Materials and Methods: A total of 142 benign predominantly cystic thyroid nodules (median volume, 12.5 mL; range, 0.4– 156 mL) in 137 patients (male:female sex ratio, 36:101; mean age ± standard deviation [SD], 49 ± 13 years) were treated with LIA after being confirmed as benign via cytology. The volume reduction rate (VRR) of the nodules and cosmetic score were evaluated during follow-up at 1, 3, and 6 months after treatment and every 6 months thereafter. A VRR of ≥ 50% at the 12-month follow-up was considered to indicate effective treatment. The associations between the clinical factors and nodular ultrasound features, including the initial nodule volume, proportion of solid components, vascularity grade and ineffective treatment (VRR of < 50% at the 12-month follow-up), and regrowth were analyzed. Results: All patients completed follow-up for at least 12 months. The average ± SD follow-up period was 32 ± 11 months (range, 12–54 months). The effective treatment rate was 73.2% (104/142), while the regrowth rate was 12.0% (17/142) at the last follow-up. Grade 2–3 intranodular vascularity in the solid components of the nodules was the only independent factor associated with ineffective treatment, with an odds ratio (reference category, grade 0–1) of 3.054 (95% confidence interval, 1.148–8.127) (p = 0.025). Conclusion: LIA is an effective treatment for predominantly cystic thyroid nodules. Grade 2–3 intranodular vascularity in the solid components of nodules is the only independent risk factor for ineffective LIA.
Alterations of Mast Cells and TGF-β1 on the Silymarin Treatment for CCl4-Induced Hepatic Fibrosis
( Dong Hwan Kim ),( Myung Hee Sohn ),( Hai Jie Yang ),( Dong Wei Yuan ),( Tae Hwan Kim ),( Kyu Shik Jeong ),( Da Hee Jeong ),( Gi Ppeum Lee ),( Won Il Jeong ),( Sun Hee Do ) 한국수의병리학회 2004 학술대회 Vol.8 No.-
Jie Dong,Rui Guo,Minjie Huang,Deqian Wang,Jiaxing Huang 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1
Chinese sacbrood virus (CSBV) is a disastrous virus that fatally threatens honeybees (Apis cerana) in China. Here, we identified the complete genome of CSBV using the rapid amplification of cDNA ends (RACE) method and compared the obtained sequences with those of other SBVs through multiple sequence alignment. The results showed that the full-length of CSBV genome from Zhejiang Province (CSBV-ZJ) is 8824 bp and contains a single, large open reading frame (ORF) that encodes 2842 amino acids flanked by 190 nucleotides (nts) of the 5′-untranslated region (UTR) and 108 nts of the 3′-UTR. The multiple genome comparison showed that SBVs have five conserved domains and that CSBV-ZJ belong to SBV type II. The phylogenetic analysis demonstrated that the CSBV-ZJ strain was similar to the CSBV from Fuzhou (CSBV-FZ) strain. Colonies collected from most of the sampling sites in the area surrounding Qiandao Lake were carriers of CSBV. The haplotypes of the VP1 gene formed a network with radiated and reticular structures. In conclusion, this characterization of the CSBV-ZJ genome will help us understand the SBV prevalence mechanism and aid the protection of honeybees.
생물학적 처리공정 내 AI(Ⅲ)계와 Fe(Ⅲ)계의 화학적 응집제 투입에 따른 인제거
전동걸 ( Dong Jie Tian ),임현숙 ( Hyun Sook Lim ),이준상 ( Joon Shang Lee ),전항배 ( Hang Bae Jun ) 충북대학교 산업과학기술연구소 2014 산업과학기술연구 논문집 Vol.28 No.1
This study compared the coagulation characteristics by coagulant through inputting A1(Ⅲ) and Fe(Ⅲ) to each unit process of biological sewage treatment processes. IN addition, this study attempted to derive the optimal operating conditions in consideration of economic feasibility. The input dose of coagulant for removal effective of phosphorus was found to be ranked in a descending order as follow: anaerobic > influent > anoxic > aerobic > effluent. In effluent with few or no suspended solids, the molar ratios for phosphors removal of A1(Ⅲ) and Fe(Ⅲ) were 1.2 and 1.24 respectively. These ratios were 15 or 20 times lower than those of anoxic and aerobic processes. The amount of sludge production was found to do ranked in a descending order as follows: anaerobic > anoxic > aerobic > influent > effluent, and this could be considered attributable to concentration of both suspended solids and phosphorus of each reactor. Thus, sludge was produced the most in anaerobic.
동시 질산화-탈질(SND) 반응을 적용한 MBR 반응조에서 질소 및 인 제거 특성
전동걸(Dong Jie Tian),임현숙(Hyun Suk Lim),안찬현(Chan Hyun An),이봉규(Bong Gyu Lee),전항배(Hang Bae Jun),박찬일(Chan Il Park) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.10
동시 질산화 탈질은 미세 용존 산소하에 한 반응조내에서 일어난다. 따라서, 본 연구에서는 인 방출을 위해 공기가 공급되는 MBR 전단에 혐기성 존을 만들어주었으며, 높은 DO 농도에서 탈질효율을 향상시켜 주기 위해서는 MBR 내에 배플을 설치하여 무산소 존이 이루어지게 하였다. 그리고 인 제거를 위한 테스트는 MBR 전단의 혐기성 반응조에 알럼 응집제를투입하여 수행하였다. 질소 제거를 위한 SND의 최적 DO 농도 도출은 MBR 내 DO 농도를 2.0, 1.5, 1.0, 0.75 mg/L의 다양한조건에서의 운전을 통해 수행하였다. 심지어 높은 알칼리성 하수라 알럼 응집제를 투입하였을 때 알칼리 용액 첨가 없이도 pH 는 7.0~8.0로 유지되었다. TCODcr와 NH₄+-N의 제거 효율은 모든 DO 농도에서 90% 이상이었다. DO 농도 2.0, 1.5, 1.0, 0.75mg/L에서의 TN 제거효율은 각각 50, 51, 54, 66%이었다. DO 농도 0.75 mg/L 조건에서 알럼을 첨가한 결과 TN 제거효율은 54%로 감소하였다. 혐기성 반응조에 알럼을 투입한 결과 TP 제거효율은 29%에서 95%로 향상되었다. 그리고 알럼 투입 후 분리막모듈의 화학적 세정 주기는 15~20일부터 40~50일으로 늘어났다. Simultaneous nitrification and denitrification (SND) occurs concurrently in the same reactor under micro dissolved oxygen (DO) conditions. Anaerobic zone was applied for phosphorus release prior to an aerated membrane bio-reactor (MBR), and anoxic zone was installed by placing a baffle in the MBR for enhancing denitrification even in high DO concentration in the MBR. Phosphorus removal was tested by alum coagulation in the anaerobic reactor preceding to MBR. DO concentration were 2.0,1.5, 1.0, 0.75 mg/L in the MBR at different operating stages for finding optimum DO concentration in MBR for nitrogen removal by SND. pH was maintained at 7.0~8.0 without addition of alkaline solution even with alum addition due to high alkalinity in the raw sewage. Both TCODcr and NH₄+-N removal efficiency were over 90% at all DO concentration. TN removal efficiencies were 50, 51, 54, 66% at DO concentration of 2.0, 1.5, 1.0, 0.75 mg/L, respectively. At DO concentration of 0.75 mg/L with addition of alum, TN removal efficiency decreased to 54%. TP removal efficiency increased from 29% to 95% by adding alum to anaerobic reactor. The period of chemical backwashing of the membrane module increased from 15~20 days to 40~50 days after addition of alum.