Introducing Soil and Plant Science: Undergraduates Learning through Experiences

Cristine Morgan,Damien J Field 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

HSP27 is Commonly Expressed in Cervical Intraepithelial Lesions of Brazilian Women

Dobo, Cristine,Stavale, Joao Norberto,Lima, Flavio De Oliveira,Ribeiro, Daniel Araki,Arias, Vitor,Gomes, Thiago Simao,Oshima, Celina Tizuko Fujiyama Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Heat shock proteins are molecular chaperones that may be constitutively present in cells protecting them from various stresses, such as extreme temperature, anoxia or chemical agents. Cervical cancer is the second most prevalent malignancy of women. In this study, we analyzed the expression of Hsp27 by immunohistochemistry in cervical intraepithelial lesions of Brazilian women, along with samples from non neoplasic lesions (NN). Cervical intraepithelial neoplasia I (CIN I), II (CIN II) and III (CIN III)/in situ carcinoma and squamous cell carcinoma (SCC) were included. Immunostaining was observed in 30 (100%) samples of NN, 46 (92%) in CIN I, 50 (100%) in CIN II, 52 (98.11%) in CIN III/CIS, and 46 (98.11%) in SCC. In group NN Hsp27 immunostaining was heterogeneous, more intense in basal and parabasal layers of the epithelium and less or absent in the intermediate and superficial layer. The majority of the samples of CIS and SCC presented strong staining in all epithelial layers. Metaplasic cells, when present, were strongly stained. In this study, Hsp27 protein was found to be commonly expressed in cervical epithelial cells.

Intraobserver and interobserver reproducibility in linear measurements on axial images obtained by cone-beam computed tomography

Nathália Cristine da Silva,Maurício Barriviera,José Luiz Cintra Junqueira,Francine Kühl Panzarella,Ricardo Raitz 대한영상치의학회 2017 Imaging Science in Dentistry Vol.47 No.1

Purpose: This study was performed to investigate the intra- and inter-observer variability in linear measurements with axial images obtained by PreXion (PreXion Inc., San Mateo, USA) and i-CAT (Imaging Sciences International, Xoran Technologies Inc., Hatfield, USA) CBCT scanners, with different voxel sizes. Materials and Methods: A cylindrical object made from nylon with radiopaque markers (phantom) was scanned by i-CAT and PreXion 3D devices. For each axial image, measurements were taken twice in the horizontal (distance A-B) and vertical (distance C-D) directions, randomly, with a one-week interval between measurements, by four oral radiologists with five years or more experience in the use of these measuring tools. Results: All of the obtained linear measurements had lower values than those of the phantom. The statistical analysis showed high intra- and inter-observer reliability (p=0.297). Compared to the real measurements, the measurements obtained using the i-CAT device and PreXion tomography, on average, revealed absolute errors ranging from 0.22 to 0.59 mm and from 0.23 to 0.63 mm, respectively. Conclusion: It can be concluded that both scanners are accurate, although the linear measurements are underestimations, with no significant differences between the evaluators.

Intraoperative discomfort associated with the use of a rotary or reciprocating system: a prospective randomized clinical trial

Aline Cristine Gomes,Adriana Jesus Soares,Erick M Souza,Alexandre Augusto Zaia,Emmanuel João Nogueira Leal Silva 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: The aim of this randomized, controlled, prospective clinical study was to evaluate patients’ intraoperative discomfort during root canal preparations in which either multi-file rotary (Mtwo) or single-file reciprocating (Reciproc) systems were used. Materials and Methods: Fifty-five adult patients, aged between 25 and 69 years old, with irreversible pulpitis or pulp necrosis participated in this study. Either the mesiobuccal or the distobuccal canals for maxillary molars and either the mesiobuccal or the mesiolingual canals for mandibular molars were randomly chosen to be instrumented with Mtwo multi-file rotary or Reciproc single-file reciprocating systems. Immediately after each canal instrumentation under anesthesia, patient discomfort was assessed using a 1 - 10 visual analog scale (VAS), ranging from ‘least possible discomfort’ (1) to ‘greatest possible discomfort’ (10). The Wilcoxon signed-rank test was used to determine significant differences at p < 0.05. Results: Little intraoperative discomfort was found in all cases. No statistically significant differences in intraoperative discomfort between the 2 systems were found (p = 0.660). Conclusions: Root canal preparation with multi-file rotary or single-file reciprocating systems had similar and minimal effects on patients’ intraoperative discomfort.

Salt stress alters the morphoanatomy and primary and secondary metabolites of the leaves and rhizomes of Curcuma longa L.

Bonacina Cristine,Nascimento Andressa Bezerra,Barbosa Letícia Neris,de Souza Silvia Graciele Hülse,Magalhães Hélida Mara 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2

During cultivation, environmental factors can cause changes in the metabolism of Curcuma longa L. that limit its development. This study evaluated the efects of diferent sodium chloride (NaCl) concentrations on the leaf anatomy, stomatal features, and biochemical activity of C. longa L. plants. The experiment was conducted in a greenhouse, using rhizomes of C. longa L. planted in plastic pots containing soil, sand, and organic matter (2:1:1). After 180 days of planting, salt stress using NaCl (50, 100, and 150 mM) was induced and a control treatment was made with water. At 150 days post treatment, the morphoanatomical variables of the leaves were evaluated, and biochemical analyses of the leaves and rhizomes were performed. The leaves exposed to intermediate salt concentrations (50 and 100 mM) had fewer hypodermic cells and larger spaces between the cells in the spongy parenchyma. In the 150 mM NaCl group, there was an increase in the total leaf thickness caused by an increase in hypodermic cells. The percentage of closed stomata increased in the 50 mM (85%) and 100 mM (80%) NaCl treatments, and the polar diameter of the cells (1.22%) was reduced in the presence of 150 mM NaCl. Biochemical analyses revealed diferent responses for rhizomes and leaves. The total sugars in the rhizomes increased in the presence of ≥50 mM NaCl, probably because of the need to generate more energy for use in secondary metabolite synthesis pathways. The antioxidative activity and phenolic compounds also increased at the highest NaCl concentrations. In conclusion, we have classifed C. longa L. as a plant that is tolerant to saline stress, owing to the metabolic balance and water-loss barrier provided by the rhizome to mitigate stress damage. Our results showed that C. longa L. plants were tolerant to the high salt concentrations tested in this study (100 and 150 mM).

Alchornea glandulosa Ethyl Acetate Fraction Exhibits Antiangiogenic Activity: Preliminary Findings from In Vitro Assays Using Human Umbilical Vein Endothelial Cells

Flávia Cristine Mascia Lopes,Ana Rocha,Ana Pirraco,Luis O. Regasini,Janaina R. Siqueira,Dulce H.S. Silva,Vanderlan S. Bolzani,Iracilda Z. Carlos,Raquel Soares 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.10

Alchornea glandulosa has traditionally been used in Brazilian folk medicine for the treatment of immune-inflammatory diseases and as an antiulcer agent to heal gastric ulcer and gastritis. Angiogenesis is a complex multistep process that consists of proliferation, migration, and anastomosis of endothelial cells and has a major role in the development of pathologic conditions, such as inflammatory diseases. To investigate a possible link between the anti-inflammatory activities and antiangiogenic effects of A. glandulosa ethyl acetate fraction (AGF), this study examined which features of the angiogenic process could be disturbed by this fraction. The antiangiogenic activity of AGF was determined in vitro by using human umbilical vein endothelial cells (HUVEC), and cell viability, proliferation, apoptosis, invasion, and capillary-like structures formation were addressed. To elucidate the mechanism of action, nuclear factor κB (NFκB), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with AGF. A significant decrease in proliferation, a relevant increase in apoptosis, and a strong reduction in invasion capacity (as assessed by bromodeoxyuridine, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling, and double-chamber assays, respectively) were observed. AGF also led to a drastic reduction in the number of capillary-like structures formed when HUVEC were cultured on growth factor–reduced Matrigel–coated plates. In addition, incubation of HUVEC with AGF resulted in reduced NFκB activity. These findings emphasize the antiangiogenic potential of AGF and support its therapeutic use for disorders that involve excessive angiogenesis, such as chronic inflammation and tumor growth.

Intraoperative discomfort associated with the use of a rotary or reciprocating system: a prospective randomized clinical trial

Gomes, Aline Cristine,Soares, Adriana Jesus,Souza, Erick M,Zaia, Alexandre Augusto,Silva, Emmanuel Joao Nogueira Leal The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: The aim of this randomized, controlled, prospective clinical study was to evaluate patients' intraoperative discomfort during root canal preparations in which either multi-file rotary (Mtwo) or single-file reciprocating (Reciproc) systems were used. Materials and Methods: Fifty-five adult patients, aged between 25 and 69 years old, with irreversible pulpitis or pulp necrosis participated in this study. Either the mesiobuccal or the distobuccal canals for maxillary molars and either the mesiobuccal or the mesiolingual canals for mandibular molars were randomly chosen to be instrumented with Mtwo multi-file rotary or Reciproc single-file reciprocating systems. Immediately after each canal instrumentation under anesthesia, patient discomfort was assessed using a 1 - 10 visual analog scale (VAS), ranging from 'least possible discomfort' (1) to 'greatest possible discomfort' (10). The Wilcoxon signed-rank test was used to determine significant differences at p < 0.05. Results: Little intraoperative discomfort was found in all cases. No statistically significant differences in intraoperative discomfort between the 2 systems were found (p = 0.660). Conclusions: Root canal preparation with multi-file rotary or single-file reciprocating systems had similar and minimal effects on patients' intraoperative discomfort.

Intraobserver and interobserver reproducibility in linear measurements on axial images obtained by cone-beam computed tomography

Silva, Nathalia Cristine da,Barriviera, Mauricio,Junqueira, Jose Luiz Cintra,Panzarella, Francine Kuhl,Raitz, Ricardo Korean Academy of Oral and Maxillofacial Radiology 2017 Imaging Science in Dentistry Vol.47 No.1

Purpose: This study was performed to investigate the intra- and inter-observer variability in linear measurements with axial images obtained by PreXion (PreXion Inc., San Mateo, USA) and i-CAT(Imaging Sciences International, Xoran Technologies Inc., Hatfield, USA) CBCT scanners, with different voxel sizes. Materials and Methods: A cylindrical object made from nylon with radiopaque markers (phantom) was scanned by i-CAT and PreXion 3D devices. For each axial image, measurements were taken twice in the horizontal(distance A-B) and vertical (distance C-D) directions, randomly, with a one-week interval between measurements, by four oral radiologists with five years or more experience in the use of these measuring tools. Results: All of the obtained linear measurements had lower values than those of the phantom. The statistical analysis showed high intra- and inter-observer reliability (p=0.297). Compared to the real measurements, the measurements obtained using the i-CAT device and PreXion tomography, on average, revealed absolute errors ranging from 0.22 to 0.59 mm and from 0.23 to 0.63 mm, respectively. Conclusion: It can be concluded that both scanners are accurate, although the linear measurements are underestimations, with no significant differences between the evaluators.

IS THIS AN AUTHENTIC PLACE? THE INFLUENCE OF AUTHENTICITY IN THE TOURIST´S DESTINATION SELECTION

Jeanne Rosalina Botelho Maciel,Eliane Cristine Francisco Maffezzolli 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

The search for authenticity is a subject that has become very common in different areas of social knowledge in recent years. The key point is to recognize what makes something authentic and which factors could influence this perception. Although the academia not yet been able to reach a consensus on the definition of what makes something authentic, some authors propose this concept in the marketing of goods and services (Beverland 2005, 2006; Beverland, Lindgreen & Vink, 2008; Alexander, 2009; Molleda, 2009; Kadirov, 2010). We propose to extend this concept to the place branding context. Destinations can be authentic when they offer tourists unique experiences. These could be interesting to be branded in order to increase tourism demand. Tourism is a sector that, despite of the uncertainties of the global economic scenario, continues to grow. According to the World Tourism Organization, in 2014 there was an increase of 4.3% in the number of tourists who traveled the world, compared to the previous year. This represents 46 million more people that revisited a destination or traveled to new places. In financial terms, the global move rose to US$ 1.5 trillion in 2014 (UNWTO, 2015). The economy of many countries depends on tourism, both in terms of GDP and employment. For instance, Seychelles, Cape Verde and Malta have respectively 21%, 16% and 13% of their GDPs coming from tourism. The same source also reveals the list of the most competitive countries in the world in terms of tourism. The top four countries are Spain, France, USA and England (WEF, 2015). Currently, considering the competitiveness in the tourism sector, it is increasingly critical the need for destinations which present a singular identity and positioning. Offering excellent options of accommodation, good attractions and historic buildings are no longer differentials. Thus, place branding can be a powerful tool to face the competitive tourism scenario (Gilmore & Pine II, 2007). This study aims to promote a measurement scale for place authenticity and verify the influence of this concept for the tourist’s destination selection. In modern societies, individuals search for their uniqueness. Taylor (2003) points out that one of the decisive aspects to be an authentic positioning is the definition of their own identity, that is, the set of attributes such as personality, physical characteristics and perception of themselves. The dilemma of authenticity and inauthenticity is already considered one of the key points of western society (Grayson & Martinec, 2004). Considering the universe of brands, an authentic brand is associated to the consistency between brand identity and brand personality. It is related to the elements and/or characteristics, which are unique. Previous published studies (Beverland, 2006; Barreto, 2008; Molleda, 2009; Kadirov, 2010; Napoli et al. 2014) proposed some dimensions to define the composing elements for authenticity perception; however, they were more focused on product authenticity. Some dimensions include product tradition, manufacturing process, product design, certifications and guarantees, price positioning, product originality, cultural symbolism, product quality, nostalgic perception and brand credibility, among others. These dimensions are not directly applicable to touristic destinations. Considering this literature gap, we decided to propose a new measurement scale specifically for places (touristic destinations). In the case of tourists, Gilmore and Pine II (2007) state that this kind of consumers search to live unique and authentic experiences. These authors emphasize that the choice of a place to visit can be considered personal reaffirmation, in the sense of having a certain personality or belonging to a particular group. The proposed model for this study states that place authenticity is guided by place tradition and place legitimacy and plays a mediating effect from place image to destination selection. For place image, we considered the natural characteristics of a touristic destination, the quality of services and the original manufacturing production of that place. These dimensions also came from the qualitative steps of this research. Previous studies (Selby & Morgan, 1996; Govers & Go, 2009) emphasize the positive impact of place image to increase consumer’s visiting desire. Before access destination selection, place image plays an important role for place authenticity perception, being that the core evaluation about a place. The first three hypothesis are: H1: The greater the perception about natural characteristics of a place, the greater the perception about place authenticity. H2: The greater the perception about the quality of services, the greater the perception about place authenticity. H3: The greater the perception about original manufacturing production, the greater the perception about place authenticity. Destination selection for vacation is related to consumer’s choice about the next place to go during his/her free time. Literature reveals that consumers’ perception about a place can be decisive for choosing or not a place for vacation (Gilmore & Pine II, 2007). Hypothesis 4 assumes that: H4: Place authenticity has a positive and significant impact on consumer’s destination selection. This study is organized in two main research stages. The first was dedicated to the developing a place authenticity measurement scale. We followed Netemeyer, Bearden and Sharma (2003) steps that include literature review, qualitative and quantitative steps. After the literature review we ran two qualitative studies with four in-depth interviews with tourism managers and eight in-depth interviews with consumers. Managers’ criteria selection was strategic public and private functions. Consumers’ criteria considered: age, gender and personal finance responsibility. The main objective of these steps was to collect more information about “what makes a place authentic” and “how we can define place image”. Initially 32 items were proposed, being 12 for place authenticity and 20 for place image. The first survey with 152 respondents was used to test the proposed scales. The second stage was defined by another survey with 152 valid respondents. We adapted from Napoli, Dickinson, Beverland, and Farrelly (2014) a 3-item scale for destination selection. The main objective of this last step was to retest the measurement scale with a new sample and test the proposed model using structural modeling equation. We used a 10-point agreement scale for all constructs. Qualtrics solutions to collect data were used in both surveys. The qualitative approach and the literature review were relevant to propose some items to measure place image and place authenticity. Previous studies emphasized the power of place image for tourism managers in order to increase destination desire for potential consumers (Selby & Morgan, 1996; Govers & Go, 2009). Echtner and Ritchie (1993) written one of the first manuscripts to promote a measurement scale to place image. It was a very robust proposition that included attribute-based images, holistic impressions, and functional, psychological, unique and common characteristics, considering a combination of structured and unstructured methodologies. Although it is a very complex scale. We proposed a new scale by listening to managers and potential tourists (consumers) about what defines the main aspects of place image. Considering place authenticity, the main characteristics that define this concept are related to place tradition and history, which are related to culture, socio-economic history and how people recognize the uniqueness of its tradition. Place legitimacy is related to the originality of place characteristics and what makes this place unique. In the first survey group, we had 152 valid responses, 64% women. Age range was from 26 to 37 years old. Main income rate (61%) was US$1600. Respondents should be the main responsible for the last destination choice. After a descriptive analysis, we ran an exploratory factor analysis (EFA) with varimax rotation and principal components as extraction method. Results showed the adequacy of the proposed scale (KMO=0.886, Bartlett's 4807.951, p<0.000). Four items were deleted considering the communalities verification (under 0.50). Three dimensions were defined: place image, which means the natural characteristics of a place (6 items, α=0.938), the quality of services (7 items, α=0.914) like infrastructure and the original manufacturing production of that place (4 items, α=0.807). Two dimensions defined place authenticity: place tradition (7 items, α=0.938) and place legitimacy (5 items, α=0.934). In the second survey group, we had 152 valid responses, 62% women. Age range was from 26 to 37 years old. Main income rate (61%) was between US$1600 and US$2500. Respondents should be the main responsible for the last destination choice. The second stage included a new survey to confirm the measurement scales and test the proposed model. After descriptive analysis, we ran a confirmatory factor analysis (CFA). Results demonstrated the adequacy of the measurement model with tolerable goodness of fit (CMIN 403.114, DF = 142, CMIN/DF= 2.839, IFI=0.916, CFI=0.915, TLI=0.886, NFI=0.876, NFI=0.835, RMSEA=0.110). Probably sample size influenced the goodness of fit. We refined the measurement scale holding the essential items for each scale, considering CFA analysis (appendix 1). The final measurement proposed is defined for the natural characteristics of a place (4 items, AVE=0.80, CONF=0.93), the quality of services (4 items, AVE=0.84, CONF=0.95), the original manufacturing production of that place (3 items, AVE=0.76, CONF=0.90). Place authenticity: place tradition (4 items, AVE=0.77, CONF=0.93) and place legitimacy (4 items, α= AVE=076, CONF=0.93). The proposed model was tested using the structural modeling equation. Results demonstrated to be suitable, with an acceptable goodness of fit (CMIN 310.219, DF = 97; CMIN/DF= 3.198; IFI=0.907, CFI=0.905, TLI=0.867, NFI=0.876, RFI=0.817, RMSEA=0.121). All hypotheses were confirmed. There is a positive impact from natural characteristics (β=0.371, p<0.000), quality of services (β=0.236, p<0.000) and original manufacturing production (β=0.597, p<0.000) on place authenticity (R²=92%). There is also a positive impact from place authenticity to destination selection (β=0.427, p<0.000, R²=18.2%). Three major results emphasize the literature contribution of this research. First, the two-dimension place authenticity measurement scale. Place tradition and place legitimacy are the core concepts to measure place authenticity. Second, the influence of place image on place authenticity. This is useful for tourism managers since place image can be treated by marketing campaigns. The better the place image the greater the place authenticity perception. This relation explains 92% of the place authenticity construct. Thirdly, the relevance of place authenticity on destination selection. Public and private investments for some touristic place can be reinforced by marketing efforts in order to increase positive aspects for place image and place authenticity. Therefore, promoting increase in the percentage of destination selection. People are looking for unique experiences in unique places.

Alpha Beta Gamma of Radiometrics for Enhancing Digital Soil Mapping

Gregory Rouze,Cristine Morgan,Alex Mcbratney 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6