카드뮴 결합 고분자량 단백질의 생화학적 특성

천기정,김봉희 충남대학교 약학대학 의약품개발연구소 1995 藥學論文集 Vol.11 No.-

The isoelelectric points of Cd-BP(Ⅰ) and Cd-BP(Ⅱ), cadmium-binding proteins, were 6.01 and 5.35, respectively. Both of them contained zinc. As for the amino acid composition. Cd-BP(Ⅰ) contained a lot of glycine and lysine but none of such aromatic amino acids as tyrosine and phenylalanine. On the other hand, Cd-BP(Ⅱ) contained leucine, histidine, asparti cacid and alanine but no aromatic amino acids.

쇠비름 수침액이 방사선 조사에 의한 생쥐의 생존율과 뇨중 아미노산 조성에 미치는 영향

천기정,김진규,이영근,김봉희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

A study has been conducted to investigate the radioprotective effects of purslane extracts (P) when I.p. injected before irradiation (R). For studying the amino acid composition , three groups of ICR mice (7-week-old) were tested. The first group I.p. injected with purslane extract for 5 days were irradiated with 6 Gy of γ-radiation (P+R). The second group was irradiated with 6 Gy without any pretreatment (R). The third group was non-irradiated control (CT). Each group divided into two groups for the survival rate study, one injected with saline (S) and the other injected with purslane extract (P) for five days and then irradiated with 8 Gy. The amino acid composition of urine samples were analyzed with HPLC for 19 days after irradiation. A few kinds of amino acids such as lysine and methionine in urine from P+R group increased in comparison with those from CT or R group. The survival rate of P group maintained much higher than that of S group during experimental period. The results obtained may support that this plant has radioprotective substances by means of life-lengthening, not of urine amino acid components.

정신보건시설 간 비교연구 -네 가지 변수를 중심으로-

전석균, 박봉길 그리스도신학대학교 출판부 2010 교수논문집 Vol.10 No.-

A Comparative Study among Mental Health Settings -Based on four variables - The purpose of this study is to compare four variables among mental health settings. In order to perform the purpose, the researcher performed questionnaire via post mail to mental health social workers employed in mental health settings(mental hospitals, community mental health centers, social rehabilitation agency) in Seoul. The result of this study showed that there were more role ambiguity, lower organizational commitment, lower supervision function, and more reduced personal accomplishment in community mental health centers, and were more depersonalization of client in social rehabilitation. And there were more role conflicts, lower organizational commitment, more depersonalization of client, and more reduced personal accomplishment when supervision was not practiced. Based on the results, implications for mental health practice were discussed.

만성 간염과 간경변증의 고해상 초음파 소견

정덕환,구봉식,최종철,박병호,남경진 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.2

흰쥐 조직에서의 카드뮴 결합 고분자량 단백질의 유도

천기정,김봉희 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

The study was carried out on the biochemical characters of Cd-BP(Ⅰ) after isolation and purification of the protein from the liver of rat injected intraperitoneally with Cd. A continued study has been doing whether Cd-BP(Ⅰ) could be induced by Cd or by other metals such as Zn and Cu. Antisera were made against the Cd-BP(Ⅰ) from NewZealand white rabbits. Carried out were γ-globulin purification, then Ouchterlony test and gel immunodiffusion test,. Cd-BP(Ⅰ) was also found in normal tissues of rat. It was induced up to a considerable level by Cd, whose induced level was higher than that of Cu or Zn treatment. The level of induction by Cu or Zn pretreatment plus Cd treatment was lower than that by simple treatment of Cu or Zn. Such a result was presumable related to the Cd toxicity.

팔로3징의 외과적 치료

천종록,전상훈,장봉현,이종태,김규태 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 비교적 드물게 보는 선천성 심질환중의 하나인 팔로3징에 대한 본원에서의 진단 및 치료 경험을 임상적으로 관찰하여 보고함으로써 향후 이 질환의 개심술 성적을 향상시키는데 기여하고자 하였다. 대상 및 방법 : 경북대학교병원 흉부외과에서 1980년 3월부터 1997년 2월 사이에 개심술을 받았던 환자들 중에서 심도자검사 및 수술소견에 의해 팔로3징으로 진단되었던 8례를 대상으로 술전증상과 진찰소견, 술전의 심초음파검사, 심도자검사 및 심혈관조영술의 소견들을 검토하였고, 수술소견으로는 폐동맥협착과 심방중격결손의 해부학적 형태, 체외순환시간과 대동맥차단시간, 수술방법, 술후 합병증 및 수술사망률 등을 조사하였다. 그리고 평균 4.8년에 걸친 술후 추적관찰을 하였다. 결과 : 술전 진단에 있어서는 2례에서 오진되어 있었고, 술후 조기의 병원사망례는 없었으나 1례에서 류마치스성으로 추정되는 다발성 심장판막질환으로 8년후 인공판막치환술을 받았는데 패혈증으로 사망하였다. 나머지 환자들은 모두 양호한 경과를 취하였다. 결론 : 이 질환의 술전 진단에 있어서 과거에는 상당한 오진이 있었음을 알 수 있었고, 술전 증상이 심한 환자가 많았던 사실을 감안하면 개심술 성적과 술후 경과는 양호한 편이었다. Eight parients with trilogy of Fallot, characterized by the combination of pulmonary stenosism, atrial septal defect(ASD) and Rt-to-Lt shunt in the atrial level in the presence of an intact ventricular septum, underwent surgical correction between March 1980 and February 1997. Four patients were male and four were female. Their ages ranged from 2 months to 20 years with a mean age of 12.1 years. Cardiac catheterization and angiocardiogram were performed in all patients and 2D-Echocardiographic studies were obtained in 4 patients before operation. The preoperative diagnoses were tetralogy of Fallot(TOF) + ASD in 2 cases, trilogy of Fallot in 6 cases had combined pulmonary stenosis. The atrial septal defect was closed by primary suture in 6 cases and patch closure was done in 2 cases. The pulmonary stenosis was correctied by valvotomy in 2 cases, valvotomy + infundibulectomy in 2 cases, valvotomy + infundibulectomy + right venr tricular outflow tract reconstruction in one case, valvotomy + infundibulectomy + transannular patching in 3 cases. No operative death resulted, but one patient expired after undergoing mitral valve replacement and tricuspid annuloplasty for mitral insufficiency, tricuspid insufficiency and pulmonary valve insufficency 8 years later. All surviving patients have remained in good condition.

감마선에 조사된 생쥐에 있어서 방사선방어효과 평가를 위한 생물학적 파라메타

천기정,김봉희,이영근,김진규 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

This study deals with the biological changes in mice after γ-irradiation. Four weeks old BALB/c mice were irradiated with 6.5Gy of γ-ray on the fifth day after oral administration of radioprotectants such as ascorbic acid, tocopherol and cysteine. Control group was irradiated with 6.5Gy without pre-administration of radioprotectors. Blood cells and sperm cells were counted and body, testis and spleen were weighed 3 days after irradiation. And also liver antioxidant activity and range of spleen immune cells were measured. Differences in most biological parameters were not clearly distinguished between experimental groups. However, the relative spleen weight, the relative testis weight and the population size of spleen immune cells such as T helper cells, B cells and macrophages measured by means of FACS showed significant difference between irradiated and radioprotectant administered group. It is concluded that the relative spleen weight, the relative testis weight and the population size of spleen immune cells are easy and useful parameters for assessing the effect of radioprotective substances and for quantifying biological damage of radiation, as well.

흰쥐 간에서의 카드뮴 결합 고분자량 단백질의 분리 및 정제(Ⅰ)

천기정,김봉희 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

We isolated two Cd-binding high molecular weight proteins from intraperitoneally cadmium injected rat liver. Molecular weight of Cd-BP(Ⅰ) purified from Sephacryl S-100 and DEAE Sepharose column chromatography and Cd-BP(Ⅱ) purified from DEAE Sepharose column chromatography and Sulphonyl Sepharose column chromatography was 33,000 and 18,400, respectively. Alcohol dehydrogenase and alkaline phosphatase acitivities were not detected from two purified proteins.

새로운 방선균의 탐색과 신규 유용 물질의 탐색 및 개발에 관한 연구

전진근,조봉금 建國大學校 附設 自然科學硏究所 1993 建國自然科學硏究誌 Vol.4 No.-

토양으로부터 690종의 방선균을 분리 그중 α-amylase inhibitor의 활성이 큰 Streptomyces를 분리하였다. α-amylase inhibitor의 최적 생산 조건은 2% soluble starch, 2% trypton, 0.05% ZnSO₄초기 배양액 pH=7.0, 배양시간 60시간, 배양온도 30℃였다. 세포벽 성분을 분석한 결과 LL-DAP형이고, 단당류 분석결과 Arabinose, Galactose, Xylose등이 존재하지 않았다 따라서 Bergey's mannual of determinative bacteriology, I.S.P strain Key, Shirling과 Gottlieb, Bergey's mannual of sysmatic bateriology에 의해 cell wall type Ⅰ에 해당되고 생리, 생화학적 특징 및 형태적 특징으로 볼 때 α-amylase inhibitor 생성균주는 Streptomyces 에 속하므로 Streptomyces sp. GG 330-2로 명명하고 Streptomyces albulus와 유사한 균주로 동정했다. 최적온도, pH 및 배지에서 배양한 여액을 FPLC로 정제하였으며 α-amylase inhibitor의 안전성은 열처리 및 pH 변화 등에서 활성이 그대로 유지되는 안정성을 보였다. Various methods for selective isolation are being used in the ecological study of Actinomycetes and the search for useful strains of these organisms. Because of the difficulties of selective isolation of Actinomycetes, we focus on new methods being improved at our laboratory, which include pretreatment of soil suspension with spore activating agents and germicides. These treatments greatly increased the counts of Actinomycetes cfu(colony forming unit) from soil, decreased bacterial counts on the isolation plate of HY agar. Our treatment(dry heat, wet heat, phenol, SDS and Yeast extract) and HY agar were useful for the highly selectable isolation of Micromonospora and Actinomycetes. 690 strains of soil microorganism were isolated from Korean soil and compost samples. α-amylase inhibitor producing micro-organisms were isolated from blue zone separated Actinomycetes Agar piece on the starch agar plate contained α-amylase. The morphological, physiochemical characteristics of each strains and their culture condition were evaluated at 30℃ with the best medium containing 2% soluble starch, 2% trypton and 0.05% ZnSO₄and the activity was obtained after 60 hours culture. The purification of inhibitors was analyzed in order to know stability of temperature and pH. At 100℃ for 60 min and the range of pH 2∼8 the activity of inhibitor were stabilized. Strains producing α-amylase inhibitors were identified to be morphologically similar to Streptomyces albulus, each of which were analyzed by means of SEM, cell wall and monosaccharides.

단세포 겔 전기영동법을 이용한 생쥐 비장 림프구 DNA 손상에 대한 비타민 C 및 시스테인의 방사선 방어효과

천기정,김진규,김봉희 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.1

The alkaline comet assay, employing a single-cell gel electrophoresis (SCGE), is a rapid, simple and sensitive technique for visualizing and measuring DNA damage leading to strand breakage in individual mammalian cells. The protecting effect of pretreatment with vitamin C and cysteine on the DNA damage of gamma ray was investigated in mice splenic lymphocytes. Vitamin C and cysteine were administered orally for five consecutive days before irradiation. Four week old ICR male mice were irradiated with 3.5Gγ of y-radiation and were sacrificed 3 days later. Spleens were taken for DNA damage examination by Comet assay and the tail moments of DNA single-strand breaks in the splenic lymphocytes were evaluated. The results show that pretreatment with vitamin C and cysteine were effective in protecting against DNA damage by gamma ray. Administration of antioxidants like vitamin C and cysteine to mice before irradiation was effective in reducing the tail moment of splenic lymphocytes DNA.