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Beata Prozorow-Krol,Agnieszka Korolczuk,Grazyna Czechowska,Maria Slomka,Agnieszka Madro,Krzysztof Celinski 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.9
The role of adenosine A3 receptors and theirdistribution in the gastrointestinal tract have been widelyinvestigated. Most of the reports discuss their role inintestinal inflammations. However, the role of adenosineA3 receptor agonist in pancreatitis has not been wellestablished. The aim of this study is (Ed note: Purposestatements should be in present tense) to evaluate theeffects of the adenosine A3 receptor agonist on the courseof sodium taurocholate-induced experimental acute pancreatitis(EAP). The experiments were performed on 80male Wistar rats, 58 of which survived, subdivided into 3groups: C—control rats, I—EAP group, and II—EAPgroup treated with the adenosine A3 receptor agonistIB-MECA (1-deoxy-1-6[[(3-iodophenyl) methyl]amino]-9H-purin-9-yl)-N-methyl-B-D-ribofuronamide at a dose of0.75 mg/kg b.w. i.p. at 48, 24, 12 and 1 h before and 1 hafter the injection of 5 % sodium taurocholate solution intothe biliary–pancreatic duct. Serum for a-amylase and lipasedeterminations and tissue samples for morphologicalexaminations were collected at 2, 6, and 24 h of theexperiment. In the IB-MECA group, a-amylase activitywas decreased with statistically high significance comparedto group I. The activity of lipase was not significantlydifferent among the experimental groups but higher than inthe control group. The administration of IB-MECA attenuatedthe histological parameters of inflammation ascompared to untreated animals. The use of A3 receptoragonist IB-MECA attenuates EAP. Our findings suggestthat stimulation of adenosine A3 receptors plays a positiverole in the sodium taurocholate-induced EAP in rats.
Beata Bialek,이재일 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.6
An all electron ab-initio method was emlpoyed to study the electronic and the magnetic properties of the (001) surface of alkaline-earth metal silicides, CaSi, SrSi, and BaSi, in the rock-salt structure. The three compounds retain their ferromagnetic metallic properties at the surface. Due to the surface effects, the magnetism of the topmost layer is changed as compared with the bulk. This is a short-range effect. In CaSi, the magnetism of the surface layer is noticeably reduced, as compared with the bulk: magnetic moments (MMs) on both Ca and Si atoms are reduced. In SrSi (001), the polarization of electrons in the surface atoms is similar to that in the bulk atoms, and the values of MMs on the component atoms in the topmost layer do not change as much as in CaSi. In BaSi (001), the magnetic properties of Si surface atoms are enhanced slightly, and the magnetism of Ba atoms is not affected considerably by the surface effect. The calculated densities of states confirm the short-range effect of the surface on the electronic properties of the metal silicides.
( Beata Krawczyk ),( Justyna Leibner Ciszak ),( Karolina Stojowska ),( Jozef Kur ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.12
This study details and examines a novel ligation-mediated polymerase chain reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4-base, 5` overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5` overhangs of the adapter and a 4- base sequence of the 3` end of the primer(s) determine a subset of the genomic restriction fragments, which are amplified by PCR. The method permits the differentiation of bacterial species strains on the basis of the different DNA band patterns obtained after electrophoresis in polyacrylamide gels stained with ethidium bromide and visualized in UV light. The usefulness of the LM-PCR/ Shifter method for genotyping is analyzed by a comparison with the restriction endonuclease analysis of chromosomal DNA by the pulsed-field gel electrophoresis (REA-PFGE) and PCR melting profile (PCR MP) methods for isolates of clinical origin. The clustering of the LM-PCR/Shifter fingerprinting data matched those of the REA-PFGE and PCR MP methods. We found that the LM-PCR/Shifter is rapid, and offers good discriminatory power and excellent reproducibility, making it a method that may be effectively applied in epidemiological studies.
A Chemometric Approach to Distribution of Selenium in Medicinal Plants Cultivated in Poland
Beata Ulewicz-Magulska,Marek Wesolowski 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.5
The aim of this study was to evaluate the level of selenium (Se) in common raw plant materials (herbs, leaves, flowers, fruits, and roots) obtained from medicinal plants cultivated in Poland. Furthermore, the relationship between the morphological part of a plant and its species was examined, and the concentration of Se in it was measured. Spectrofluorimetric determination of Se in 148 samples of 44 plant species revealed that the majority of the plants contained Se at a level from several to several tens of μg/kg dry weight (d.w.). A relatively high Se concentration, in the order of 50 μg/kg d.w., was found in Majoranae herba, Crataegi fructus, and Lini semen. An especially high Se level, >100 μg/kg d.w., was found in only three plants—Equiseti herba, Farfarae folium, and Cichorii radix. Application of a nonparametric Kruskal–Wallis analysis of variance test indicates that the morphological variable influences the level of Se in the studied groups of raw plant materials. The mean concentration of Se in fruits differs significantly from that found in flowers and roots. However, there was no statistically significant difference between the Se content in herbs and leaves and its levels in fruits, flowers, and roots. Cluster analysis and principal component analysis calculations show that it is possible to relate the Se concentration in plant material to the plant species and botanical family of the medicinal plant in only a few cases. Analysis of average Se levels in the plant species showed that the plants belonging to the Apiaceae and Labiatae botanical families are more Se-rich than those belonging to Rosaceae.