Porosity and pore size distribution in high-viscosity and conventional glass ionomer cements: a micro-computed tomography study

Neves Aline Borburema,Lopes Laísa Inara Gracindo,Bergstrom Tamiris Gomes,Silva Aline Saddock Sá da,Lopes Ricardo Tadeu,Neves Aline de Almeida 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4

Objectives This study aimed to compare and evaluate the porosity and pore size distribution of high-viscosity glass ionomer cements (HVGICs) and conventional glass ionomer cements (GICs) using micro-computed tomography (micro-CT). Materials and Methods Forty cylindrical specimens (n = 10) were produced in standardized molds using HVGICs and conventional GICs (Ketac Molar Easymix, Vitro Molar, MaxxionR, and Riva Self-Cure). The specimens were prepared according to ISO 9917-1 standards, scanned in a high-energy micro-CT device, and reconstructed using specific parameters. After reconstruction, segmentation procedures, and image analysis, total porosity and pore size distribution were obtained for specimens in each group. After checking the normality of the data distribution, the Kruskal-Wallis test followed by the Student-Newman-Keuls test was used to detect differences in porosity among the experimental groups with a 5% significance level. Results Ketac Molar Easymix showed statistically significantly lower total porosity (0.15%) than MaxxionR (0.62%), Riva (0.42%), and Vitro Molar (0.57%). The pore size in all experimental cements was within the small-size range (< 0.01 mm3), but Vitro Molar showed statistically significantly more pores/defects with a larger size (> 0.01 mm3). Conclusions Major differences in porosity and pore size were identified among the evaluated GICs. Among these, the Ketac Molar Easymix HVGIC showed the lowest porosity and void size.

Edema and hematoma after local anesthesia via posterior superior alveolar nerve block: a case report

Aline Louise Nascimento Marques,Sidney R. Figueroba,Marco Antonio Tridapalli Mafra,Francisco Carlos Groppo 대한치과마취과학회 2022 Journal of Dental Anesthesia and Pain Medicine Vol.22 No.3

Although rare, complications can occur with anesthetic procedures. The posterior superior alveolar nerve (PSAN) block anesthetic technique has a high success rate, but positive aspiration can cause bruising, transient diplopia, blurred vision, and temporary blindness in approximately 3% cases. When edema occurs, it is occasionally massive, especially in the infratemporal fossa, and the resulting hematoma is usually unsightly. A 20-year-old woman presented with massive edema followed by hematoma in the upper right jaw immediately after PSAN block administration, which subsequently spread to the oral mucosa. The patient did not report any complications during the anesthetic procedure. However, after the injection was administered, the patient experienced anesthetic sensations, which rapidly evolved to facial edema. There was mild pain, but without intraoral or extraoral bleeding. The patient was prescribed medicines and instructed to perform contrast therapy. Although hematomas and edema are rare, they are difficult to prevent. The choice of local anesthetic and appropriate application of the anesthetic technique can minimize their occurrence.

Green carbon dots synthesized from Chlorella Sorokiniana microalgae biochar for chrome detection

Aline C.C. Pena,Lucas Manique Raymundo,Luciane F. Trierweiler,Mariliz Gutterres 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

In the quest to find an economical and accurate method of detecting toxic metals as well as finding anapplication for biochar as a fuel, this work used microalgae biochar to sinter Carbon Dots (CDs) thatcan be used as pathways for metallic ions detection. Carbon Dots from microalgae biochar (generatedin pyrolysis) were synthesized through thermochemical depolymerization with KMnO4 at different concentrations. After this step, the purification of the nanoparticles was performed. The characterization ofCDs was performed through fluorescence intensity, Zeta Potential, Fourier Transform InfraredSpectroscopy (FTIR), and atomic force microscopy (AFM). The results showed that all samples exhibitedfluorescence, samples synthesized with KMnO4 at concentrations of 8.54 % and 10.0 % (CD-MBK06 andCD-MBK07, respectively) showed the highest fluorescence intensity and good stability. When addingCr (VI) and Cr (III) to the CD-MBK06 and CD-MBK07 samples, it is possible to notice that there was fluorescencequenching as the metal ion concentration increased, except for the CD-MBK07 sample for Cr(III), at a length of 350 nm, indicating that this sample shows selectivity for extinction only for Cr (VI).

Fingerprint and authenticity roasted coffees by 1H-NMR: the Brazilian coffee case

Aline Theodoro Toci,Marcos Vinı´cius de Moura Ribeiro,Paulo Roberto Aparecido Bueno de Toledo,Nivaldo Boralle,Helena Redigolo Pezza,Leonardo Pezza 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1

With globalization, it has become necessary to adopt policies to regulate the coffee market, addressing problems including the authenticity and traceability of products. It is therefore important to establish methodologies that can help to safeguard the interests of producer countries and add value to products. For this purpose, the use of NMR combined with multivariate statistical procedures can be an attractive option. The aim of this study was to develop a fast and effective technique, using 1H NMR coupled with multivariate statistics, to create a fingerprint of roasted coffees, distinguishing them according to the main Brazilian producer regions. Several compounds suitable for differentiating roasted coffees were identified in the fingerprint. Discriminant analysis revealed good distinction among the samples. The compounds catechol, trigonelline, caffeine, and n-methylpyridine were most important for the differentiation. The findings should assist coffee-producing countries in adopting measures to protect their markets and to add value to coffee products.

Dietary Polydextrose Prevents Inflammatory Bowel Disease in Trinitrobenzenesulfonic Acid Model of Rat Colitis

Aline Witaicenis,Andre´a C. Fruet,Leticia Salem,Luiz C. Di Stasi 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.6

Inflammatory bowel disease (IBD) is a multifactorial intestinal disorder that involves interactions among the immune system, genetic susceptibility, and environmental factors, especially the bacterial flora. Polydextrose, a polysaccharide constituted by 90% nondigestible and nonabsorbable soluble fibers, has several physiological effects consistent with those of dietary fibers, including proliferation of colon microflora. Because sulfasalazine presents serious side effects through long-term use at high doses, the aim of the present study was to evaluate the preventative effect of polydextrose on trinitrobenzenesulfonic acid-induced intestinal inflammation and its effects on the intestinal anti-inflammatory activity of sulfasalazine. Results indicated that polydextrose and its association with sulfasalazine present an anti-inflammatory effect that reduces myeloperoxidase activity, counteracts glutathione content, and promotes reductions in lesion extension and colonic weight/length ratio.

Genipa americana (Rubiaceae) Fruit Extract Affects Mitogen-Activated Protein Kinase Cell Pathways in Human Trophoblast–Derived BeWo Cells: Implications for Placental Development

Aline Oliveira da Conceição,Maria Helena Rossi,Fernando Faustino de Oliveira,Larissa Takser,Julie Lafond 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.5

Genipa americana L. (Rubiaceae) is a fruit tree and a traditional medicine used to treat anemia, icterus, asthma, and liver and spleen problems. The aim of the present study was to verify the effect of G. americana fruit ethanolic extract on the mechanism for proliferation and differentiation of trophoblast-like cells. Qualitative analysis of G. americana fruit extract was performed, and BeWo cells, a well-established placental choriocarcinoma cell line that can undergo differentiation, were used to analyze cell viability and proliferation. Methods consisted of cytotoxic and proliferation measurement, detection of release of human chorionic gonadotrophins, cell fusion observation, and evaluation of cell-signaling pathways (production of cyclic adenosine monophosphate and phosphorylation of mitogen-activated protein kinases [MAPKs]). A stock solution of the extract was diluted in Ham's F-12 medium with 10% fetal bovine serum at concentrations ranging from 50 to 1000 μg/mL. Cells treated with dimethylsulfoxide, forskoline, and MAPK inhibitors (PD98059 or SB203580) were used as a control. Forskoline was used to induce the differentiation state in BeWo cells. Phytoanalysis indicated the presence of steroids only. Results showed that the G. americana fruit extract did not cause any cytotoxicity or interference in cell differentiation. However, a significant antiproliferative state related to inhibition and reactivation of ERK1/2 and p38 MAPK in BeWo cells was seen. These results suggest that steroids from G. americana may affect placental cell regulation.

유럽의 노동이사제 : 법제 및 실제 운영

Aline Conchon 한국노동연구원 2022 국제노동브리프 Vol.20 No.5

Effect of St. John's Wort Standardized Extract and Hypericin on In Vitro Placental Calcium Transport

Aline Oliveira da Conceição,Larissa Takser,Julie Lafond 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4

Hypericum perforatum L., known as St. John's wort (SJW), is widely used in human therapeutics for wound healing and to treat depression; however, its recommendation during pregnancy is controversial. Hypericin, a polycyclic quinone isolated from this plant, has been studied and used to standardize the plant extracts. The objective of the present study was to examine the effect of SJW and hypericin on in vitro placental Ca2+ transport. Cell viability, human chorionic gonadotrophin (hCG) production, Ca2+ uptake, and Ca2+ transport proteins expression analysis were conducted using the JEG-3cell line. Toxicity of SJW was seen at high concentrations (≥150μg/mL), but no effect on hCG production was observed using SJW (25μg/mL) or hypericin (7.5 and 75ng/mL). The results showed that cells treated with both SJW and hypericin exhibited increased intracellular Ca2+ concentration after long-term (24-hour) but not short-term (10-minute) period incubation. A significant decrease in translationally controlled tumor protein Ca2+ handling protein was seen only with SJW-treated cells. Hypericin increased the protein expression of the transient receptor potential vanilloid 6 Ca2+ channel and 28-kDa calcium-binding protein and decreased that of plasma membrane Ca2+-ATPase 1/4. In conclusion, SJW and hypericin can increase the trophoblast internal Ca2+ concentration through regulating the protein expression of the Ca2+ transport system, and their intake during pregnancy is still a point of concern.

Intraoperative discomfort associated with the use of a rotary or reciprocating system: a prospective randomized clinical trial

Aline Cristine Gomes,Adriana Jesus Soares,Erick M Souza,Alexandre Augusto Zaia,Emmanuel João Nogueira Leal Silva 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: The aim of this randomized, controlled, prospective clinical study was to evaluate patients’ intraoperative discomfort during root canal preparations in which either multi-file rotary (Mtwo) or single-file reciprocating (Reciproc) systems were used. Materials and Methods: Fifty-five adult patients, aged between 25 and 69 years old, with irreversible pulpitis or pulp necrosis participated in this study. Either the mesiobuccal or the distobuccal canals for maxillary molars and either the mesiobuccal or the mesiolingual canals for mandibular molars were randomly chosen to be instrumented with Mtwo multi-file rotary or Reciproc single-file reciprocating systems. Immediately after each canal instrumentation under anesthesia, patient discomfort was assessed using a 1 - 10 visual analog scale (VAS), ranging from ‘least possible discomfort’ (1) to ‘greatest possible discomfort’ (10). The Wilcoxon signed-rank test was used to determine significant differences at p < 0.05. Results: Little intraoperative discomfort was found in all cases. No statistically significant differences in intraoperative discomfort between the 2 systems were found (p = 0.660). Conclusions: Root canal preparation with multi-file rotary or single-file reciprocating systems had similar and minimal effects on patients’ intraoperative discomfort.

근로자의 기업 이사회 참여에 관한 유럽의 제도

Aline Conchon 한국노동연구원 2013 국제노동브리프 Vol.11 No.3