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히야신스의 화경 기내배양시 자구의 재생과 생장에 미치는 생장조절제의 영향
정용모,김진희,이용문,이영병,이경순 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4
히야신스 3품종(Jan Bos, Pink Pearl, Carnegie)의 화경절편들을 조직배양을 통해 자구와 뿌리의 형성능력을 조사해 본 결과 품종에 따라 Murashige & Skoog의 기본 고체배지 또는 IAA 3 ?? + BA 0.1 ??, IAA 3 ?? + BA 1 mg ·L-1, NAA 0.1 ?? + BA 0.1 ??, 그리고 NAA 0.1 ?? + BA 1 mg· L-1의 혼용배지들에서 발근이 된 자구들을 바로 획득할 수 있었는데, 화경의 착화부 조직은 비착화부 조직에 비해 자구의 재생율이 높고 재생기간도 빨랐다. 반면에, 후자의 조직은 전자의 조직에 비해 발근율이 높고 발근기간도 훨씬 빠른 경향을 보였다. 그리고 발근이 된 자구들을 분리시켜 계대배양하는 과정에서 IAA 1 ?? + BA 0.1 ?? 또는 NAA 0.5 ?? + BA 0.1??의 혼용처리는 자구들의 생장을 대체로 촉진시켰지만, 뿌리 생장과 신초의 발육은 억제했다. 그리고 부정근의 형성이 용이치 않은 분리된 자구들은 무처리구에서 발근이 양호하였다. Tissue culture was applied to investigate the regeneration of bulblets on inflorescence stalk segments of Hyacinthus orientalis cvs. Jan Bos, Pink Pearl, and Carnegie. Bulblets with roots were obtained directly on Murashige and Skoog's medium or on basal medium supplemented with 3 ?? IAA + 0.1 ?? BA or 3 ?? IAA+1 ?? BA or 0.1 ?? NAA + 0.1 ?? BA or 0.1 ?? NAA + 1 ?? BA according to cultivars. And the bulblets regeneration ability of inflorescence stalk tissue with inflorescence was higher and the days required for the formation of bulblets was shorter than that of inflorescence satlk tissue without inflorescence, on the contrary, the root formation ability of the latter was higher and the days required for the formation of roots of the latter was much faster than the former. During the subcultures of seperate bulblets with roots, the bulblet growth was generatreatments of 1 ?? IAA + 0.1 ?? BA or 0.05 NAA + 0.1 ?? BA. And the root formation of seperate bulblets without adventitious roots was good on basal medium without growth substances.
金鍾喆,李慶順,鄭大守 東亞大學校 大學院 1997 大學院論文集 Vol.22 No.-
This study was carried out to estimate the effects of planting density on the yield and seed components of soybean (Glycine max (L.) Merrill), Bokwangkong and Muhankong. According to the results, there were no significant differences of planting density on days to flowering and maturity, and 100 grain weight, respectively. Increasing the planting density, stem length, the number of branches and yields were respectively increased, although stem diameter was decreased. The composition rate of protein showed no regular tendency in the two soybean varieties, although oil components was higher in the dense plot. In the analysis of amino acid, both tested soybeans had a high level of glutamic acid and aspartic acid, and the composition rate of valine, arginine and methionine were respectively getting higher according as the increment of planting density. On the other hand, histidine and lysine was lower as the increment of planting density. For the variation of minerals, calcium content was lower, however, the content of phosphoric acid was preferably increased according to the dense planting.