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진재광,신태균,Jin, Jae-kwang,Shin, Tae-kyun 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1
To investigate the involvement of protein kinase C(PKC) isoenzyme in the testes which control spermatogenesis and hormone secretion, we examined cellular distribution of four types of PKC $\alpha$, ${\beta}I$, ${\delta}$ and ${\theta}$ in the horse testes using PKC antisera by western blot analysis and immunohistochemistry. By the western blot analysis, PKC $\alpha$ and ${\beta}I$ were detected at 82KD, while PKC ${\delta}$ and ${\theta}$ were detected at 80KD in the testes of both juvenile and adult horses. In juvenile horse, PKC $\alpha$, ${\delta}$ and ${\theta}$ except ${\beta}I$ were not detected in the cells of the testes, whereas PKC ${\beta}I$ was immunoreacted with only in spermatocytes. In adult, PKC $\alpha$, ${\beta}I$, ${\delta}$ and ${\theta}$isoenzymes were localized in interstitial cells of the testes. In the seminiferous tubules, PKC ${\beta}I$ is localized in spermatocyte, spermatid and spermatozoa, while PKC ${\delta}$ is localized only in spermatids. We suggest that this is a first report to localize PKC in the testes of horse and PKC isoenzymes are upregulated in the cells of horse testes depending on ages. These findings also suggest that certain PKC isoenzyme plays an important role in the signal transduction of spermatogenic cells and interstitial cells in horse testes.
제주 김녕리 궤내기 동굴 유적에서 출토된 뼈유물의 고고학적 연구
신태균,진재광,이차수,Shin, Tae-kyun,Jin, Jae-kwang,Lee, Cha-soo 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
The animal bone-remains excavated with earthwares at Cheju Kimnyungri cave site were investigated morphologically and osteometrically. The number of bone remains were 1706 pieces by morphological criteria. Based on the classification of bony pieces excavated in the cave site, the important animal species in Cheju island during the early Tamra period (presumably between A.D.0 - A.D. 500) was as follows; wild boar(75%), deer(17%), cattle(6%), and horse in small percentage. The excavated bone remains imply that the major fauna of animal species are composed of three species, including Sus scrofa, Cervus nippon and Bos taurus. These data suggests that the archaeological remains such as bone pieces are good indicators of the fauna animals, and of zoological entity in the island.
추출된 이종 크롬 친화성 세포의 이식에 의한 마우스에서의 통증 완화 효과
한영민,이종필,황형식,송준호,박상구,박석주,진재광,최은경,김용선,안명수,Han, Young-Min,Lee, Jong-Phil,Hwang, Hyung-Sik,Song, Joon-Ho,Park, Sang-Koo,Park, Suk-Ju,Jin, Jae-Kwang,Choi, Eun-Kyoung,Kim, Yong-Sun,Ahn, Myung-Soo 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.4
Objective : Adrenal medullary chromaffin cells are known to release analgesic substances such as opioides and catecholamines. Transplantation of them is a novel method that challenges current approaches in treating chronic pain. The transplantation of xenogeneic chromaffin cells into the central nervous system(CNS) supply antinociception in animals. In this study, we investigated the analgesic effects of rat adrenal medullary chromaffin cells transplanted into the CNS of the mouse. To study the antinociceptive efficacy of transplanted chromaffin cells, the survival of rat adrenal medullary chromaffin cells transplanted into the CNS of mouse was determined. Methods : The adrenal medullary chromaffin cells isolated from rat were transplanted into the striatum of mouse. These cells were confirmed of the release of Met-enkephalin and Leu-enkephalin by HPLC, and immunoblots for tyrosine hydroxylase(TH). Two weeks after transplantation, we performed immunohistochemistry for TH to determine the survival of implanted cells and assessed pain sensitivity at the same time. Results : The isolated rat adrenal medullary chromaffin cells were positive for anti-TH antibody and released Met-enkephalin and Leu-enkephalin more than rat endothelial cells. Transplanted rat chromaffin cells were stained with anti-TH antibody in striatum of mouse after 2 weeks. Pain sensitivity was reduced on the chromaffin cell-transplanted mouse compared to endothelial cell-transplanted mouse by the hot plate test. Conclusion : These results suggest that the rat chromaffin cells were suitably transplanted into the CNS of mouse. This approach could be used as a therapy for reducing of chronic pain induced by cancer or neuronal injury.
유산망아지에서 말herpesvirus 의 면역조직학적 및 immunogold 기법에 의한 전자현미경적 진단
신태균,이두식,진재광,임윤규,조길재 濟州大學校 農科大學 動物科學硏究所 1997 動物科學論叢 Vol.12 No.1
To investgate the causative Virus in aborbon of horse in Cheju, we examined the spleen of affected foal by immunohtochemistq and immunogold electron microscopy using EHV - specific antisera. Immunohistochemical staining revealed that a large number of EHV immunoreadive cells were deteded in the red pulp as well as in the sinudoidal cells in the affected spleen. By the immunogold electron microxopy(EM) of crude preparation of EHV culture, electron dense gold paricles were labelled along the envelope of amorphous virus parhcles with vanng sizes of 50-200nm. We suppose that immunohistochemical method is good for the diagnosis of tissues in EHV infections, and immunogold EM is recommended for the study of virus dia gnosis of fluid specimen as well as the ultrastustural study.