프로테오믹스 기술의 현황 : 정량 비교 프로테엄 분석툴

최종순,Choi, Jong-Sun 한국유전체학회 2007 유전체소식 Vol.7 No.3

Sensitivity of AutoPap Primary Screening System with Location-Guided Screening in Uterine Cervical Cytology

최종순,장회숙,김희숙,전이경,김혜선,박지영,박인서,홍성란,Choi, Jong-Sun,Jang, Hoi-Sook,Kim, Hy-Sook,Chun, Yi-Kyeong,Kim, Hye-Sun,Park, Ji-Young,Park, In-Sou,Hong, Sung-Ran The Korean Society for Cytopathology 2003 대한세포병리학회지 Vol.14 No.2

Objective: The sensitivity of the AutoPap Primary Screening System with Location-Guided Screening (AutoPap LGS) for Identifying atypical cells in cervicovaginal smears was evaluated. Methods: Two hundred forty one slides with atypical cervical cytology randomly sampled were rescreened both manually and by the AutoPap LGS. The AutoPap LGS localized the atypical cells as 15 fields of view(FOVs), which were reexamined by manual review. The sensitivity was also evaluated in accordance with the cellularity of the smears. Results: The AutoPap LGS successfully processed 232 out of 241 slides. The sensitivity of the AutoPap LGS identifying the atypical cells in successfully processed slides was 97.4%(226/232). The false negative rate was 2.6%(6/232). There was no false negative case on high grade squamous intraepithelial lesion (HSIL) or squamous cell carcinoma(SCC) smears in the AutoPap LGS. The FOVs localized the diagnostic-atypical cells in 97.8%(221/226). The number of diagnostic-atypical FOVs was increased in higher-degree of atypical cytology. The AutoPap LGS localized the atypical cells in 100% of adequately cellular smears and in 92.5% even in low cellular smears. Conclusion: The AutoPap LGS showed relatively good sensitivity to detect atypical cells. It can be a valuable system to localize atypical cells, especially in HSIL or cancer slides, even in smears with low cellularity.

DOE JGI 최신 연구 보고서 요약 (2002-2005)

최종순,Choi Jong-Sun 한국유전체학회 2006 유전체소식 Vol.6 No.1

Powder Blasting 에 의한 유리의 미세 홈 가공시 노즐 주사횟수의 영향

김광현,최종순,박동삼,Kim, Kwang-Hyun,Choi, Jong-Sun,Park, Dong-Sam 대한기계학회 2002 大韓機械學會論文集A Vol.26 No.7

The old technique of sandblasting which has been used for decoration of glass surface has recently been developed into a powder blasting technique for brittle materials such as glass, silicon and ceramics, capable of producing micro structures larger than $100{\mu}$ m. This paper describes the performance of powder blasting technique in micro-line grooving of glass and the effect of the number of nozzle scanning on the depth and width of line groove. Experimental results showed that increasing the no. of nozzle scanning resulted in the increase of depth and width in grooves. Increase of width which may cause several problems in the precision machining results from wear of mask film. Therefore, well-controlled masking process is the most important factor for micro machining of glass with accuracy.

난소의 원발 투명세포암종의 세포소견

박지영,김혜선,최종순,Park, Ji-Young,Kim, Hye-Sun,Choi, Jong-Sun 대한세포병리학회 2006 대한세포병리학회지 Vol.17 No.1

The objective of this study was to evaluate the cytomorphologic features of histologically confirmed clear cell carcinoma of the ovary and to evaluate the applicability of scrape or fine-needle aspiration cytology in making an intraoperative diagnosis. We reviewed scrape or fine-needle aspiration cytology findings in tissues taken from 6 patients with clear cell carcinoma of the ovary. The cytologic diagnosis was based primarily on findings in alcohol-fixed, hematoxylin-eosin (H-E) stained smears. The formation of material resembling a basement membrane was a characteristic finding in these smears. This extracellular hyaline material was stained light pink with H-E and was frequently found within tumor cell clusters as well as in the background material. Multinucleated giant cells were found occasionally. Each tumor cell had an abundant, clear, or granular cytoplasm with a distinct cellular membrane. Scrape cytology is a simple and rapid supportive method and could be helpful in diagnosing clear cell carcinoma of the ovary, especially when marked artifacts appear in the frozen section.

젊은 여성의 수지에 발생한 골격외 연골종(Extraskeletal Chondroma): 증례 보고

김진영,김민규,최종순,Kim, Jin Young,Kim, Min Kyu,Choi, Jong-Sun 대한근골격종양학회 2013 대한골관절종양학회지 Vol.19 No.2

골격외 연골종은 골이나 골막과의 연결이 없이 초자연골을 주성분으로 하는 연부조직의 연골종양을 이른다. 골격외 연골종은 매우 드물게 발병하며, 대부분 수부나 족부의 관절근처의 연부조직 및 건 활액막 근처에서 흔하게 관찰된다. 본 증례에서는 저자들이 경험한 젊은 여성의 수지에서 발생한 골격외 연골종 1예를 보고하고자 한다. Extraskeletal chondroma is a benign soft tissue tumor which is composed of hyaline cartilage but arises from the fibrous stroma rather than from mature cartilaginous or osseous tissue. Extraskeletal chondroma is relatively rare and occurs most frequently in the soft tissue around the joints of hands and feet. We present one case of extraskeletal chondroma in a finger of a young woman.

얇은 부분층 조갑상 이식을 통한 조갑하 외골종의 치료: 2예 보고

오인석,김창희,최종순,이상형,Oh, In Suk,Kim, Chang Hee,Choi, Jong-Sun,Lee, Sang Hyeong 대한근골격종양학회 2012 대한골관절종양학회지 Vol.18 No.2

조갑하 외골종으로 nail bed의 파괴와 손톱의 변형이 동반된 2명의 환자를 대상으로 수술 후 발생하는 발톱 변형에 초점을 두고 수술적 치료를 시행하였다. 조갑상을 손상시킨 병변에 대해 직접 절개를 통해 병변을 제거하였고 제거 후 조갑상의 결손 부위가 넓어 얇은 부분층 조갑상 이식을 시행하였고 만족할 만한 결과를 얻었다. 부위가 넓어 얇은 부분층 조갑상 이식을 통한 조갑상 결손부위의 치료는 공여부를 비교적 잘 보전하면서 표면이 매끄럽고 접촉성이 좋으며 비교적 정상에 가까운 형태의 발톱을 다시 얻는 데 효과적인 방법이다. 따라서 병변의 크기가 큰 조갑하 외골종의 절제 후 발생한 조갑상 결손의 치료에 좋은 치료방법으로 사료된다. We reviewed the clinical features of 2 patients who underwent surgery for subungal exostosis, focusing on postoperative deformity of the nail. The lesion destroyed the nail bed and was excised with a direct approach. then thin split-thickness sterile matrix graft was done after excision of the tumor because the defect of the nail bed was large. Good postoperative appearance of the nail was obtained by thin split-thickness sterile matrix graft. The use of thin split-thickness sterile matrix graft for the replacement of a nail bed defect can regain a smooth, adherent, and normal-looking nail and avoid donor-site morbidity. Thin split-thickness toe-nail bed graft is a good choice for the prevention of postoperative deformity.

자궁경부 세포검사에서 임신과 관련된 세포학적 변화

전이경,장회숙,김혜선,홍성란,최종순,박지영,조정숙,성석주,양재혁,김희숙,Chun, Yi-Kyeong,Jang, Hoi-Sook,Kim, Hye-Suu,Hong, Sung-Ran,Choi, Jong-Sun,Park, Ji-Young,Cho, Jung-Sook,Seong, Seok-Ju,Yang, Jae-Hyug,Kim, Hy-Sook 대한세포병리학회 2004 대한세포병리학회지 Vol.15 No.2

Due to insufficient clinical information, most cervicovaginal smears from pregnant or postpartum women have been screened without regard to pregnancy-related cytological changes. Here, we have reviewed 116 abnormal cervicovaginal smears from 103 pregnant and postpartum women. Initial cytological diagnoses revealed the following: 9 cases of high-grade squamous intraepithelial lesions (HSIL), 8 cases of low-grade squamous intraepithelial lesions (LSIL), 85 cases of atypical squamous cells of undetermined significance (ASCUS), and 14 cases involving atypical glandular cells of undetermined significance (AGUS). 31 cases upon review, involved pregnancy-related cytological changes, comprising 25 cases of decidua cells, 4 cases of Arias-Stella reaction, and 2 cases of cedidual cells coupled with Arias-Stella reaction. Interpretation errors were detected in 14 cases: 13 cases of decidual cells interpreted as either ASCUS favor reactive or ASCUS ruled out HSIL, and one case of Arias-Stella reaction was interpreted as ASCUS ruled out HSIL. Decidual cells and degenerated glandular cells with Arias-Stella reaction can result in diagnostic mistakes. In order to avoid misdiagnosis and unnecessary surgeries, both clinicians and pathologists must be aware of the pregnancy-related cytological changes. The clinician should also always inform the pathologist on the pregnancy status of the patient.

황색육아종으로 오인된 비전형섬유황색종

최윤석 ( Yun Seok Choi ),임원석 ( Won Suk Lim ),진상윤 ( Sang Yun Jin ),이준호 ( Joon Ho Lee ),이승호 ( Seung Ho Lee ),최종순 ( Jong Sun Choi ),김어진 ( Eo Jin Kim ),이애영 ( Ai Young Lee ) 대한피부과학회 2011 대한피부과학회지 Vol.49 No.4

Atypical fibroxanthoma is an uncommon spindle cell tumor that has a clinically benign course despite the presence of bizarre xanthoma cells. A 90-year-old female presented with a 2-month history of an ulcerative plaque on the right cheek. The initial punch biopsy revealed a benign histological appearance with prominent xanthomatous cells, but the specimen excised afterwards showed malignant features with a spindle and epithelioid cell proliferation with pleomorphism and atypia. The tumor cells stained positively for vimentin and CD68, they were partially positive for CD99 and CD10 and they were negative for CD34, S100, p63, desmin and actin. We report here on a case of atypical fibroxanthoma masquerading as xanthogranuloma. (Korean J Dermatol 2011;49(4):360∼364)

가토모델에서 Lipo-PGE1이 복합조직이식편의 미세혈관신생에 미치는 영향

박지웅,어수락,조상헌,최종순,김어진,Park, Ji-Ung,Eo, Su-Rak,Cho, Sang-Hun,Choi, Jong-Sun,Kim, Eo-Jin 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.6

Purpose: The survival of composite graft is dependent on three steps, (1) plasmatic imbibitions, (2) inosculation, and (3) neovascularization. Among the many trials to increase the survival rate of composite graft, prostaglandin E1 (PGE1) has beneficial effects on the microcirculatory level with vasodilating, antithrombotic, anti-inflammatory and neoangiogenic properties. Lipo-PGE1 which is lipid microspheres containing PGE1 had developed to compensate the systemic and local side effects of PGE1. This study was proposed to determine whether Lipo-PGE1 administration enhanced the survival of composite graft through neovascularization quantitatively in a rabbit ear model. Methods: Fourteen New Zealand White Rabbits each weighing 3~4 kg were divided in two groups: (1) intravenous Lipo-PGE1 injection group and (2) control group. A $2{\times}1\;cm$ sized, full-thickness rectangular composite graft was harvested in each auricle. Then, the graft was reaaproximated in situ using a 5-0 nylon suture. For the experimental group, $3{\mu}g$/kg/day of Lipo-PGE1 ($5{\mu}g$/mL) was administered intravenously through the marginal vein of the ear for 14 days. The control group was received no pharmacologic treatment. On the 14th postoperative day, composite graft of the ear was harvested and immunochemistry staining used Monoclonal mouse anti-CD 31 antibody was performed. Neoangiogenesis was quantified by counting the vessels that showed luminal structures surrounded by the brown color-stained epithelium and counted from 10 random high-power fields (400x) by independent blinded observer. Statistical analysis (Wilcoxon Signed Ranks test for nonparametric data) was performed using SPSS v12.0, with values of p<0.05 considered significant. Results: The mean number of the microvessels was $15.48{\pm}8.65$ in the experimental group and $9.82{\pm}7.25$ in the control group (p=0.028). Conclusion: The use of Lipo-PGE1 facilitated the neoangiogenesis, resulted in the improvement of the survival rate of graft. On the basis of this results, we could support wider application of Lipo-PGE1 for more effective therapeutic angiogenesis and successful survival in various cases of composite graft in the human.