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Determination of Sesamin in Sesame Dregs by High Performance Liquid Chromatography
Hye Min Kim(김혜민),Jeong Min Lee(이정민),Jun Yeon Park(박준언),Sullim Lee(이설림),Sanghyun P. Lee(이상현) 한국원예학회 2008 원예과학기술지 Vol.26 No.2
고성능액체크로마토그래피를 이용하여 참깨의 주요 항산화물질인 sesamin의 깻묵 중 함량을 정량 분석하였다. 분석조건으로 메탄올과 탈이온화수의 동일농도 용매조성(70:30)으로 역상 칼럼 시스템을 사용하였으며, UV 290㎚ 파장을 이용하여 확인하였다. 분석결과 깻묵 중 sesame의 함량은 한국산의 경우는 1.45㎎·g<SUP>-1</SUP>이었고, 중국산의 경우는 1.60㎎·g<SUP>-1</SUP>이었다. 이는 깻묵에 참깨의 유효성분인 sesamin이 다량 함유하였음을 입증하며, 새로운 천연자원소재의 재활용 가능성을 제시해준다. High performance liquid chromatography (HPLC) was used for the analysis of sesamin, the main antioxidant compound of sesame (Sesamum indicum L.), in sesame dregs. Reverse-phase system using a binary isocratic of methanol and deionized water (70:30) and UV detection at 290 ㎚ were performed. The contents of sesamin in the sesame dregs from Korea and China were 1.45 and 1.60 ㎎·g<SUP>-1</SUP>, respectively. The assay was successfully applied to quantify sesamin in the sesame dregs and to present the recycling possibilities of inefficiently used resources as agricultural by-products.
대추나무, 초피나무, 치자나무, 잣나무의 정유성분 GC/MS 분석
이정민(Jeong Min Lee),한샘(Saem Han),이설림(Sullim Lee),박준언(Jun Yeon Park),김혜민(Hye Min Kim),이상현(Sanghyun P. Lee) 한국원예학회 2008 원예과학기술지 Vol.26 No.3
유실수로부터 산업적 용도를 찾고자 대추나무, 초피나무, 치자나무, 잣나무 등으로부터 정유성분을 GC와 GC/MS의 분석법에 따라 분석하였다. 유실수 중 대추나무의 열매에서는 13-methyloxacyclotetradecane-2,11-dione(27.84%), 초피나무의 잎에서는 α-pinene(33.68%), 치자나무의 꽃에서는 2,5,5-trimethyl-1,3,6-heptatriene(32.00%), 잣나무의 잎에서는 α-pinene(20.52%)의 성분함량이 높았다. 이 분석결과는 유실수로부터 향수 및 음료 등 산업화의 가능성을 제시해준다. To search for the industrial application of fruit trees, chemical analysis of volatile constituents from Zizyphus jujuba var. inermis, Zanthoxylum piperitum, Gardenia jasminoides for. grandiflora, and Pinus koraiensis was conducted by GC and GC/MS spectrometric analysis. The chemical composition of the volatile constituents from the fruit trees was confirmed by the aid of Wiley/NBS Library and RI indice searches. The major constituents were 13-methyloxacyclotetradecane-2,11-dione (27.84%) from the fruits of Z. jujuba var. inermis, α-pinene (33.68%) from the leaves of Z. piperitum, 2,5,5-trimethyl-1,3,6-heptatriene (32.00%) from the flowers of G. jasminoides for. grandiflora, and α-pinene (20.52%) from the leaves of P. koraiensis. These major constituents were successfully applied to present the possibilities of industrial applications for the developments of perfumes and beverages from fruit trees.
강활(羌活)과 위령선(威靈仙)의 항염증 상승작용에 관한 연구
김승주 ( Seung Ju Kim ),김호경 ( Ho Kyoung Kim ),천진미 ( Jin Mi Chun ),양원경 ( Won Kyung Yang ),전명숙 ( Myeong Sook Cheon ),성윤영 ( Yoon Young Sung ),박준언 ( Jun Yeon Park ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.4
Objective: Oriental medicines have been combined oriental medical theory which based on the seven modes of emotions. Notopterygium incisum (N. incisum) and Clematis manshurica (C. manshurica) have been used as an anti-rheumatic and analgesic medicine for the treatment of rheumatism, headache, cold, etc. In this study, we evaluate the synergistic anti-inflammatory effect of N. incisum and C. manshurica. Method: To evaluate the synergistic anti-inflammatory effect of a herbal mixture N. incisum and C. manshurica, we examined the changed ear thickness in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema model after topical application of herbal mixture. In addition, the levels of markers for inflammation, such as tumor necrosis factor (TNF)-a, interleukin (IL)-1b, prostaglandin G2 (PGE2), and nitric oxide (NO) were measured by ELISA assay and Griess reagent in lipopolysaccharide-stimulated Raw 264.7 cells. Results: Our results showed that aqueous extracts of N. incisum and C. manshurica combination significantly inhibited the mouse ear edema induced by TPA. Moreover, the aqueous extracts of N. incisum and C. manshurica combination exhibited synergistic effects in down-regulating TNF-a, IL-1b, PGE2 level, but not NO. Conclusions : This study suggested that combined treatment of N. incisum and C. manshurica, based on seven methods in prescription compatibility, has a synergistic effect in down-regulating inflammatory response both in vitro and in vivo models.