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- 저자 : ( Shu Ping Mo ) (검색결과 3 건)
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Synthesis and Curing of AUT-PNIMMO with Three Functional Groups
Xiaochuan Wang,Ping Li,Xianming Lu,Hongchang Mo,Minghui Xu,Ning Liu,Yuanjie Shu 한국고분자학회 2019 폴리머 Vol.43 No.4
T-PNIMMO with three functional groups was synthesized by cationic ring opening polymerization of NIMMO (3-nitratomethyl-3-methyloxetane) in the presence of TMP (trimethylolpropane) catalyzed by BF3·OEt2. Then AUTPNIMMO was synthesized by T-PNIMMO and allyl isocyanate. The polymers were characterized by FTIR, 1H NMR, 13C NMR, and their thermal stability was estimated by DSC. Moreover, tensile testing was used to evaluate the mechanical properties of polyurethane elastomer based on T-PNIMMO and toluene-2,4-diisocyanate (TDI), and isoxazoline elastomer based on AUT-PNIMMO and tetramethyl-terephthalobisnitrile oxide (TTNO). This showed an increase of tensile strength from 2.0 to 4.0 MPa and elongation at break from 150% to 500%, respectively. These results indicated that AUTPNIMMO exhibited a good thermal stability, had satisfactory mechanical properties and was expected to be used in the composite solid propellant and polymer bonded explosives.
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( Sheng Rong Liu ),( Qing Ping Wu ),( Ju Mei Zhang ),( Shu Ping Mo ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3
ε-Poly-L-lysine (ε-PL) is a homopolymer of L-lysine molecules connected between the epsilon amino and alpha carboxyl groups. This polymer is currently used as a natural preservative in food. Insufficient biomass is a major problem in ε-PL fermentation. Here, to improve cell growth and ε-PL productivity, various nitrogen-rich nutrients were supplemented into flask cultures after 16 h cultivation, marking the onset of ε-PL biosynthesis. Yeast extract, soybean powder, corn powder, and beef extract significantly improved cell growth. In terms of ε-PL productivity, yeast extract at 0.5% (w/v) gave the maximum yield (2.24 g/l), 115.4% higher than the control (1.04 g/l), followed by soybean powder (1.86 g/l) at 1% (w/v) and corn powder (1.72 g/l) at 1% (w/v). However, supplementation with beef extract inhibited ε-PL production. The optimal time for supplementation for all nutrients examined was at 16 h cultivation. The kinetics of yeast-extract-supplemented cultures showed enhanced cell growth and production duration. Thus, the most commonly used two-stage pH control fed-batch fermentation method was modified by omitting the pH 5.0-controlled period, and coupling the procedure with nutrient feeding in the pH 3.9-controlled phase. Using this process, by continuously feeding 0.5 g/h of yeast extract, soybean powder, or corn powder into cultures in a 30 L fermenter, the final ε-PL titer reached 28.2 g/l, 23.7 g/l, and 21.4 g/l, respectively, 91.8%, 61.2%, and 45.6% higher than that of the control (14.7 g/l). This describes a promising option for the mass production of ε-PL.
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( Liu Sheng Rong ),( Qing Ping Wu ),( Ju Mei Zhang ),( Shu Ping Mo ),( Xiao Juan Yang ),( Chun Xiao ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
ε-Poly-L-lysine (ε-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of Llysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce ε-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more ε-PL than those immobilized on synthetic sponge, and sugarcane bagasse. Moreover, loofah sponge supported the maximum biomass. Hence, loofah sponge was chosen for cell immobilization. Meanwhile, the ion-exchange resin D152 was employed for ISA. The loofah sponge-immobilized cells produced 0.54 ± 0.1 g/l ε-PL, which significantly increased to 3.64 ± 0.32 g/l after combining with ISA through the addition of resin bags. The free cells with ISA using the dispersed resin yielded 2.73 ± 0.26 g/l of ε-PL, an increase from 0.82 ± 0.08 g/l. These data illustrate that the proposed combination method improved production most significantly compared with either immobilization or ISA only. Moreover, the immobilized cells could be repeatedly used and an ε-PL total amount of 8.05 ± 0.84 g/l was obtained. The proposed combination method offers promising perspectives for ε-PL production.
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