Anti-Oxidative and Anti-Inflammation Activities of Pork Extracts

Juae Gil,Dongwook Kim,Seok Ki Yoon,Jun Sang Ham,Aera Jang 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.2

This study was conducted to evaluate the antioxidative and anti-inflammatory effects of boiled pork powder (BPP) and hot water extract powder (HWEP) from 4 cuts of meat from Landrace × Yorkshire × Duroc (LYD). The highest DPPH radical scavenging activities determined were from BPP of Boston butt (13.65 M TE) and HWEP of loin (19.40 M TE) and ham (21.45 M TE). The 2,2-azino-bis-3-ethylbenzothiazoline- 6-sulfonic acid (ABTS) radical scavenging activities of BPP from shoulder ham (39.28 M TE) and ham (39.43 M TE) were higher than those of other meat cuts, while HWEP of ham exhibited the highest ABTS radical scavenging activity. A higher oxygen radical absorbance capacity was determined for BPP from ham (198.35 M TE) and in HWEP from loin (204.07 M TE), Boston butt (192.85 M TE), and ham (201.36 M TE). Carnosine content of BPP and HWEP from loin and were determined to be 106.68 and 117.77 mg/g on a dry basis, respectively. The anserine content of BPP (5.26 mg/g, dry basis) and HWEP (6.79 mg/g, dry basis) of shoulder ham exhibited the highest value as compared to the extracts from the other meat cuts. The viability of RAW 264.7 cells was increased with increasing HWEP from loin and ham treatment. In addition, the expression of IL-6 and TNF-α was significantly reduced by HWEP from loin and ham, in a dose dependent manner. These results suggested that boiled pork and hot water extract of pork have antioxidative and cytokine inhibitory effects.

Anti-Oxidative and Anti-Inflammation Activities of Pork Extracts

Gil, Juae,Kim, Dongwook,Yoon, Seok-Ki,Ham, Jun-Sang,Jang, Aera Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.2

This study was conducted to evaluate the antioxidative and anti-inflammatory effects of boiled pork powder (BPP) and hot water extract powder (HWEP) from 4 cuts of meat from Landrace × Yorkshire × Duroc (LYD). The highest DPPH radical scavenging activities determined were from BPP of Boston butt (13.65 M TE) and HWEP of loin (19.40 M TE) and ham (21.45 M TE). The 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of BPP from shoulder ham (39.28 M TE) and ham (39.43 M TE) were higher than those of other meat cuts, while HWEP of ham exhibited the highest ABTS radical scavenging activity. A higher oxygen radical absorbance capacity was determined for BPP from ham (198.35 M TE) and in HWEP from loin (204.07 M TE), Boston butt (192.85 M TE), and ham (201.36 M TE). Carnosine content of BPP and HWEP from loin and were determined to be 106.68 and 117.77 mg/g on a dry basis, respectively. The anserine content of BPP (5.26 mg/g, dry basis) and HWEP (6.79 mg/g, dry basis) of shoulder ham exhibited the highest value as compared to the extracts from the other meat cuts. The viability of RAW 264.7 cells was increased with increasing HWEP from loin and ham treatment. In addition, the expression of IL-6 and TNF-α was significantly reduced by HWEP from loin and ham, in a dose dependent manner. These results suggested that boiled pork and hot water extract of pork have antioxidative and cytokine inhibitory effects.

Cyclic Voltammetry Stripping Analysis to Determine Iodide Ion Concentration in Cu Plating Bath

Yoon, Young,Ham, Yu Seok,Kim, Tae Young,Choe, Seunghoe,Kim, Jae Jeong The Electrochemical Society 2018 Journal of the Electrochemical Society Vol.165 No.5

<P>Accurate monitoring of an electroplating bath's chemical balance is a key factor for maintaining its performance during a long-term plating operation. In this paper, a method is suggested to measure the concentration of iodide ion (I-), an inorganic leveler for through-silicon via (TSV) filling, based on its electrochemical response. During the operation of plating, I-was consumed via the reaction with Cu+ (Cu+ + I -> CuI), an oxidation reaction (2I(-) -> I-2 + 2e), as well as a physical incorporation. The I- concentration decrease resulted in a degradation of the bath, while the major byproducts (CuI and I-2) rarely influenced on bath performance. In order to monitor the I(-)concentration by cyclic voltammetry stripping (CVS) analysis, the electrochemical response of I(-)was examined at various conditions. I- suppressed the Cu electrodeposition rate; this response was dependent on the mass transport of I- and the applied potential of cathode. A subsequent effective coverage analysis revealed that not only I- but also Cu(I) iodide (CuI) was a key inhibitor, demonstrating that the inhibition of I- becomes weaker at a negative potential. With a responsive curve (RC)-CVS analysis conducted at an optimized condition, a linear relationship between the real and measured concentrations could be found, irrespective of other additives' concentrations. The method suggested in this paper enabled the direct monitoring of the I- concentration in a Cu plating bath. (C) 2018 The Electrochemical Society.</P>

담석의 병인 및 담즙검사의 의의에 관한 연구

윤석부,이옥찬,함준수 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.1

Two major type gallstones have been described which have different characteristics and etiologies, cholesterol and pigment gallstone. Fromation of cholestrol gallstones depend upon 2 factors, cholesterol superstaturation and nucleation, and it has been proposed that cholesterol gallstones originates from microcrystals of cholesterol. Pigment stones, esp. calcium bilirubinate stones occur most commonly in association with chronic infectious cholangitis that is almost unique to Orient. The pigment is present entirely as unconjugated bilirubte to the formation of pigment stones by producign β-glucuronidase and thus deconjugating bilirubin diglucuronide to unconjugated bilirubin which in turn combines with calcium, leading to stone formation. Bile samples were obtained by Entero-Test, centrifuged and a single drop was immediately examined microscopically, and bacteria isolated from bile were cultured and the β-glucurondase activity were determined. The results were as follows: 1. Total 51 patients were studies; 18 GB stones, 18 CBD stones, and 15 controls. 2. Of the 18 GB stones, 15 (83%) had CMC (Cholesterol Monohydrate Crystal) and 4(22%) of the 18 CBD stones had CMC. (p<0.01 3(17%) of 18 GB stones and 11(61%) of 18 CBD stones had CBG (Calcium Bilirubinate Granule). (p<0.01) 3. No significant difference was found in bacterial incidence between GB and CBD stones. Most frequently isolated bacteria was Escherichia coli and it was more common in CBD stones than in GB stones. (p<0.05) 4. β-glucuronidase activity was found only in CBD stones and was 100% in Escherichia coli. These result suggest that crystalization and β-glucuronidase activity secondary to bacterial infection have an important role in the formation of cholesterol and pigment gallstone, and microscopic bile examination may be helpful in predicting the presence of gallstones.

Off-Time Control of <i>LLC</i> Resonant Half-Bridge Converter to Prevent Audible Noise Generation Under a Light-Load Condition

Yoon, Ho-Young,Lee, Hyeon-Seok,Ham, Seok-Hyeong,Choe, Hyung-Jin,Kang, Bongkoo Institute of Electrical and Electronics Engineers 2018 IEEE transactions on power electronics Vol.33 No.10

<P>This paper presents a control method that can prevent audible noise generation in an <I>LLC</I> resonant half-bridge dc–dc converter under a light-load condition, while achieving the same input voltage range and power-conversion efficiency <I>η<SUB>e</SUB></I> as the burst-control method that generates an audible noise. The proposed method reduces switching and conduction losses at light load by skipping several pairs of switch-control pulses, while varying the switching frequency less than the normal-control method does. This skip-control method enables the <I>LLC</I> resonant converter to have a large magnetizing inductance, and to have high <I>η<SUB>e</SUB></I> over a wide range of load variation. At an input voltage of 385 V, an output voltage of 24 V, and a resonant frequency of 180 kHz, the proposed method achieved <I>η <SUB>e</SUB></I> ≥ 85.46% for an output power range of 7.2–360 W; the highest <I>η<SUB>e </SUB></I> was 96.08% at <TEX>$P_o$</TEX> = 336 W.</P>

Dual Bias Modulator for Envelope Tracking and Average Power Tracking Modes for CMOS Power Amplifier

Ham, Junghyun,Jung, Haeryun,Bae, Jongsuk,Lim, Wonseob,Hwang, Keum Cheol,Lee, Kang-Yoon,Park, Cheon-Seok,Yang, Youngoo The Institute of Electronics and Information Engin 2014 Journal of semiconductor technology and science Vol.14 No.6

This paper presents a dual-mode bias modulator (BM) for complementary metal oxide semiconductor (CMOS) power amplifiers (PAs). The BM includes a hybrid buck converter and a normal buck converter for an envelope tracking (ET) mode for high output power and for an average power tracking (APT) mode for low output power, respectively. The dual-mode BM and CMOS PA are designed using a $0.18-{\mu}m$ CMOS process for the 1.75 GHz band. For the 16-QAM LTE signal with a peak-to-average power ratio of 7.3 dB and a bandwidth of 5 MHz, the PA with the ET mode exhibited a poweradded efficiency (PAE) of 39.2%, an EVM of 4.8%, a gain of 19.0 dB, and an adjacent channel leakage power ratio of -30 dBc at an average output power of 22 dBm, while the stand-alone PA has a PAE of 8% lower at the same condition. The PA with APT mode has a PAE of 21.3%, which is an improvement of 13.4% from that of the stand-alone PA at an output power of 13 dBm.

Luteolin induces intrinsic apoptosis via inhibition of E6/E7 oncogenes and activation of extrinsic and intrinsic signaling pathways in HPV-18-associated cells

HAM, SUNYOUNG,KIM, KI HONG,KWON, TAE HO,BAK, YESOL,LEE, DONG HUN,SONG, YONG SEOK,PARK, SU-HO,PARK, YUN SUN,KIM, MAN SUB,KANG, JEONG WOO,HONG, JIN TAE,YOON, DO-YOUNG Spandidos Publications 2014 ONCOLOGY REPORTS Vol.31 No.6

Impact of Fatty Liver on Acute Pancreatitis Severity

Yoon, Seung Bae,Lee, In Seok,Choi, Moon Hyung,Lee, Kyungjin,Ham, Hyoju,Oh, Hyun Jin,Park, Se Hwan,Lim, Chul-Hyun,Choi, Myung-Gyu Hindawi 2017 Gastroenterology Research and Practice Vol.2017 No.-

<P><I>Aim</I>. Acute pancreatitis is typically a mild disease, but some patients develop severe courses. Fatty liver changes are seen in patients with acute pancreatitis, but its clinical significance has not been well-studied. We aimed to investigate the relationship between fatty liver and the severity of acute pancreatitis. <I>Methods</I>. Unenhanced CT images of patients with acute pancreatitis were retrospectively reviewed by a radiologist, and mean hepatic and splenic attenuation was measured in Hounsfield units (HU). Fatty liver was defined as mean hepatic/splenic HU < 1. <I>Results</I>. Among 200 patients, fatty liver was found in 67 (33.5%) and nonfatty liver in 133 (66.5%). Compared with patients without fatty liver, the severity of pancreatitis and levels of serum C-reactive protein were higher in fatty liver patients. The prevalence of local complications, persistent organ failure, and mortality were also higher in patients with fatty liver. Even after adjusting for age, sex, body mass index, and cause of pancreatitis, fatty liver was significantly associated with moderately severe or severe acute pancreatitis. <I>Conclusions.</I> Fatty liver may play a prognostic role in acute pancreatitis. Fatty liver could be incorporated into future predictive scoring models.</P>

Inhibitory Activity of Garlic Fermented by Pediococcus pentosaceus KACC 91419 against Antibiotic-resistant Pathogens

Ham, Jun-Sang,Lee, Seung-Gyu,Kim, Min-Kyung,Oh, Mi-Hwa,Jeong, Seok-Geun,Kim, Dong-Hun,Lee, Se-Hyung,Chae, Jong-Pyo,Lee, Ji-Yoon,Kang, Dae-Kyung Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9

The aim of this study was to screen lactic acid bacteria for the fermentation of garlic and to assess the increase in inhibitory activity of garlic fermented against antibiotic-resistant pathogens for use as an animal feed supplement. We screened 45 strains of lactobacillus for the fermentation of garlic. Of these strains, 23 showed similar growth rates with or without allicin. Cultures of the 23 strains were mixed with an equivalent amount of garlic juice and incubated overnight at $37^{\circ}C$. The three strains with the lowest pH values were Lactobacillus paracasei KCTC 3169, L5 strain, and L. reuteri SW. Garlic juice fermented by the L5 strain more strongly inhibited antibiotic-resistant pathogenic bacteria than L. paracasei KCTC 3169, L. reuteri SW, or garlic juice itself. By examining carbohydrate utilization, morphologic properties and 16S rRNA gene sequences, we identified the L5 strain as Pediococcus pentosaceus and deposited it in the name of P. pentosaceus KACC 91419 into the Korea Agricultural Culture Collection. To identify the antimicrobial compound from the garlic filtrate fermented by P. pentosaceus KACC 91419, we fractionated P. pentosaceus KACC 91419 culture on a C18 column and checked the antimicrobial activity of fractions A6 to A10. Only fraction A9 showed inhibitory activity on Staphylococcus aureus. Comparing the mass spectra of the fractions with and without antimicrobial activity, we observed a single dominant product ion (m/z 157.99) from the fraction showing antimicrobial activity. Its molecular mass (157.99) was 2 atomic mass units less than that of allicin (162.02). This suggests that allicin might be converted to its derivative, which has antimicrobial activity, during fermentation by P. pentosaceus KACC 91419.

Powerful Usage of Phylogenetically Diverse Staphylococcus aureus Control Strains for Detecting Multidrug Resistance Genes in Transcriptomics Studies

Ham, Jun-Sang,Lee, Seung-Gyu,Jeong, Seok-Geun,Oh, Mi-Hwa,Kim, Dong-Hun,Lee, Tae-Heon,Lee, Bo-Young,Yoon, Sook-Hee,Kim, Hee-Bal Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.1

Staphylococcus aureus is an important human pathogen responsible for life-threatening septicemia, endocarditis, and toxic shock syndrome. Although positive (MRSA; ATCC 33591) and negative (MSSA; ATCC 25923) control strains have been used for various pathogenesis or assay studies, little is known about the genomic structure of the strains, and there has been little genome-wide expression analysis. Phylogenetic analyses revealed that ATCC 33591 and ATCC 25923 are the most genetically diverse strains of the 15 S. aureus genomes studied. Microarray analysis showed that the most significantly upregulated group of MRSA genes was the transport group, which includes ATP-binding cassette (ABC) transporters, the two-component system, and the phosphotransferase system. Analysis of the KEGG pathway showed that ABC transporters and the two-component system were the most significantly altered in MRSA. Transcriptional profiling showed a clear difference in gene expression between MRSA and MSSA due to the great genetic distance between the two control strains. Therefore, we suggest that use of the two control strains in comparative genomics or transcriptomics studies would facilitate the identification of major genes for drug resistance in S. aureus.