Preparation of Reduced Graphene Oxide and Copper Sulfide Nanoplates Composites as Efficient Photothermal Agents for Ablation of Cancer Cells

Chaofan Hu,Yingliang Liu,Jianhua Rong,Qingqing Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8

A novel strategy toward one-pot hydrothermal synthesis of copper sulfide nanoplates-decorated reduced graphene oxide (RGO–CuS) composites was developed. CuS nanoplates were successfully loaded onto the surface of RGO in the hydrothermal procedure, which was confirmed by transmission electron microscopy, UV-Vis–NIR and Raman spectroscopy. The as-synthesized RGO–CuS composites showed pronounced enhanced optical absorbance in near infrared (NR) region and higher photothermal conversion efficiency than noncomposite GO and CuS nanoplates. The RGO–CuS materials were used in photothermal ablation of cancer cells with a 980 nm laser irradiation and showed improved performance than CuS nanoplates.

Colorimetric H2O2 Detection Using Ag-Nanoparticle-Decorated Silica Microspheres

Zhikun Zhang,Qingqing Liu,Yumin Liu,Ran Qi,Lilong Zhou,Zhengjie Li,Jimmy Yun,Runjing Liu,Yongqi Hu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.02

In view of the importance of convenient and rapid H2O2 detection for biological analysis, we herein propose Ag nanoparticle (NP)-decorated silica microspheres as a probe for instant and non-enzymatic on-site colorimetric detection of H2O2. The surface hydroxyl groups of silica microspheres were reacted with (3-mercaptopropyl)trimethoxysilane to afford thiolated microspheres that subsequently bind Ag NPs. The oxidation of residual –SH groups on the silica surface to –S–S– moieties in the presence of H2O2 induces the aggregation of decorated microspheres and is accompanied by a color change. Sensor response is found to be proportional to H2O2 concentration in the range from 100 nM to 1 mM, with UV–Vis and colorimetric detection limits determined as 10 -8 M and 10 -5 M, respectively. The developed platform is successfully used to detect H2O2 in simulated human urine and is, therefore, concluded to be sufficiently stable and selective for practical applications.

Effect of Primary Holes Structure on Flow Characteristic and Combustion Performance of High Temperature Rise Combustor Based on RQL

Jian Chen,Jianzhong Li,Qingqing Dong,Jiutian Xue,Ge Hu 한국항공우주학회 2023 International Journal of Aeronautical and Space Sc Vol.24 No.3

To meet the qualification of temperature rise and reduce emissions, a high temperature rise combustor which is based on RQL (rich-burn/quick-quench/lean-burn) under different primary holes structure had been used to investigate the flow characteristic and combustion performance. The flow field was measured by PIV (particle imaging velocimetry) under different primary holes structure at normal temperature and pressure, and the combustion and emissions performances were simulated under the same conditions in this paper. The results show that the flow field, the airflow mixing effectiveness in the quench zone airflow, the distribution of temperature, the emission and the combustion efficiency change obviously with the primary holes structure. The maximum central recirculation zone height with staggered structure (Case-II) is about 23% higher than that with symmetrical structure. The central recirculation zone length is reduced by 36% and the velocity gradient in the rich zone is increased when the primary holes structure is symmetrical with three holes (Case-I). The insufficient penetration of primary jets has a negative impact to the efficient mixing of the quench zone airflow. Therefore, the symmetrical structure of primary holes is used, increasing the number of holes while reducing the area of single-hole will have a negative impact on the efficient mixing of the quench zone airflow. In addition, when the primary holes structure is symmetrical with four holes (Case-III), the combustion efficiency was reduced by 0.07% and 0.03%, and the maximum emission index of NO was increased by 14% and 19%, respectively, compared with the Case-I and Case-II. On the contrary, the maximum emission index of CO and soot were reduced in rich zone.

Bayesian-theory-based Fast CU Size and Mode Decision Algorithm for 3D-HEVC Depth Video Inter-coding

( Fen Chen ),( Sheng Liu ),( Zongju Peng ),( Qingqing Hu ),( Gangyi Jiang ),( Mei Yu ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.4

Multi-view video plus depth (MVD) is a mainstream format of 3D scene representation in free viewpoint video systems. The advanced 3D extension of the high efficiency video coding (3D-HEVC) standard introduces new prediction tools to improve the coding performance of depth video. However, the depth video in 3D-HEVC is time consuming. To reduce the complexity of the depth video inter coding, we propose a fast coding unit (CU) size and mode decision algorithm. First, an off-line trained Bayesian model is built which the feature vector contains the depth levels of the corresponding spatial, temporal, and inter-component (texture-depth) neighboring largest CUs (LCUs). Then, the model is used to predict the depth level of the current LCU, and terminate the CU recursive splitting process. Finally, the CU mode search process is early terminated by making use of the mode correlation of spatial, inter-component (texture-depth), and inter-view neighboring CUs. Compared to the 3D-HEVC reference software HTM-10.0, the proposed algorithm reduces the encoding time of depth video and the total encoding time by 65.03% and 41.04% on average, respectively, with negligible quality degradation of the synthesized virtual view.

Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis

Qian Liu,Bishao Sun,Jiang Zhao,Qingqing Wang,Fan An,Xiaoyan Hu,Zhenxing Yang,Jie Xu,Mingjia Tan,Longkun Li 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The Piezo1 channel is a mechanotransduction mediator, and Piezo1 abnormalities have been linked to several clinical disorders. However, the role of the Piezo1 channel in cystitis-associated bladder dysfunction has not been documented. The current study aimed to discover the functional role of this channel in regulating bladder activity during cyclophosphamide (CYP)-induced cystitis. One hundred four female rats were randomly assigned to the control, CYP-4h, CYP-48h and CYP-8d groups. CYP successfully induced acute or chronic cystitis in these rats. CYP treatment for 48h or 8d significantly increased Piezo1 channel expression in bladder interstitial Cajal-like cells (ICC-LCs), and the increase in CYP-8d rats was more prominent. In addition, 2.5 μM Grammostola spatulata mechanotoxin 4 (GsMTx4) significantly attenuated bladder hyperactivity in CYP-8d rats by inhibiting the Piezo1 channel in bladder ICCLCs. Furthermore, by using GsMTx4 and siRNA targeting the Piezo1 channel, we demonstrated that hypotonic stressinduced Piezo1 channel activation significantly triggered Ca2+ and Na+ influx into bladder ICC-LCs during CYPinduced chronic cystitis. In addition, the Piezo1 channel functionally interacted with the relatively activated reverse mode of Na+/Ca2+ exchanger 1 (NCX1) in bladder ICC-LCs from CYP-8d rats. In conclusion, we suggest that the functional role of the Piezo1 channel in CYP-induced chronic cystitis is based on its synergistic effects with NCX1, which can significantly enhance [Ca2+]i and result in Ca2+ overload in bladder ICC-LCs, indicating that the Piezo1 channel and NCX1 are potential novel therapeutic targets for chronic cystitis-associated bladder hyperactivity.

Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice

Qian Liu,Zhou Long,Xingyou Dong,Teng Zhang,Jiang Zhao,Bishao Sun,Jingzhen Zhu,Jia Li,Qingqing Wang,Zhenxing Yang,Xiaoyan Hu,Longkun Li 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are confirmed to be expressed in bladder interstitial Cajal-like cells (ICC-LCs), but little is known about their possible role in cystitis-associated bladder dysfunction. The present study aimed to determine the functional role of HCN channels in regulating bladder function under inflammatory conditions. Sixty female wild-type C57BL/6J mice and sixty female HCN1-knockout mice were randomly assigned to experimental and control groups, respectively. Cyclophosphamide (CYP)-induced cystitis models were successfully established in these mice. CYP treatment significantly enhanced HCN channel protein expression and Ih density and significantly altered bladder HCN1 channel regulatory proteins. Carbachol (CCH) and forskolin (FSK) exerted significant effects on bladder ICC-LC [Ca2+]i in CYP-treated wild-type (WT) mice, and HCN1 channel ablation significantly decreased the effects of CCH and FSK on bladder ICC-LC [Ca2+]i in both naive and CYP-treated mice. CYP treatment significantly potentiated the spontaneous contractions and CCH (0.001–10 μM)-induced phasic contractions of detrusor strips, and HCN1 channel deletion significantly abated such effects. Finally, we demonstrated that the development of CYP-induced bladder overactivity was reversed in HCN1 / mice. Taken together, our results suggest that CYP-induced enhancements of HCN1 channel expression and function in bladder ICC-LCs are essential for cystitis-associated bladder hyperactivity development, indicating that the HCN1 channel may be a novel therapeutic target for managing bladder hyperactivity.