Dopaminergic Regulation of Anxiety and Stress Coping Responses of Mice Lacking Type 5 Adenylyl Cyclase

Han, Pyung-Lim The Korean Society for Integrative Biology 2004 Korean journal of biological sciences Vol.8 No.S

Dopaminergic modulation of stress coping responses of type 5 adenylyl cyclase knockout mice

Pyung-Lim Han(한평림) 한국실험동물학회 2004 한국실험동물학회 학술발표대회 논문집 Vol.2004 No.-

Alzheimer`s disease animal models and their use in AD drug-candidate screen

( Pyung Lim Han ) 한국응용약물학회 2007 학술대회 Vol.2007 No.1

공정시스템 이동현상 화학공정안정 : 바이온 계면활성제를 이용한 원유 중의 황화합물 가용화에 간한 연구

한지원,임종주,김병홍,신평균,이석규,박상권 한국화학공학회 2000 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.38 No.2

본 연구에서는 가용화제로 polyoxyethylene(POE)계 비이온 계면활성제를 사용하여 원유 중에 포함되어 있는 황화합물의 가용화도를 X-ray spectrometer를 이용하여 측정하였다. 실험에서 사용한 비이온 계면활성제들 중에서는 가장 소수성 계면활성제의 가용화력이 가장 우수하였으며, 온도 증가에 따라 가용화도가 증가함을 알 수 있었고 1 wt% 이상의 계면활성제 농도 조건에서 가용차도가 급격히 증가함을 알 수 있었다. 특히 동일한 조건에서 2차 알코올 결합을 가진 Tergitol series 계면활성제는 1차 알코올 결합을 가진 Neodol series 계면활성제와 비교하여 가용화도가 우수하였으며, cosurfactant로서 알코올을 첨가하거나 전해질을 첨가할 경우 모두 황화합물의 가용화도가 증가함을 알 수 있었다. 원유 양을 증가시켜 가용화 실험을 수행한 결과, 계면활성제의 오일 상으로의 분배(partitioning)현상은 계면활성제가 소수성일수록 또는 온도가 증가함에 따라 더욱 두드러지게 나타났으며, 탈황 미생물 성장 영향 실험에서 계면활성제 첨가가 탈황 미생물의 성장에 큰 영향을 끼치지 않음을 확인할 수 있었다. In this study, polyoxyethylene(POE) nonionic surfactants were used in order to enhance the solubilization of sulfur compounds contained in the crude oil and the solubilized sulfur contents in the aqueous surfactant solutions were measured by X-ray sulfur spectrometer. The most hydrophobic surfactant among used during this study showed the maximum solubilization capacity for the sulfur compounds in the crude oil and the solubilization of sulfur compounds was found to be increased with temperature and to be abruptly increased at above 1 wt% surfactant solutions. It was found that Tergitol series surfactants showed higher solubilizing capacity than Neodol series surfactants presumably due to the disruption of the regular packing in the hydrocarbon region of the surfactant aggregates. The addition of a cosurfactant such as alcohol and/or an electrolyte increased the solubilization of sulfur compounds in the crude oil. It was found that partitioning phenomena were shown to be significant with an hydrophobic surfactant at high temperature and the growth of sulfur reducing microorganisms was not greatly affected by the addition of nonionic surfactants.

포인트 클라우드 데이터 기반 군집형 건물 솔리드 모델 자동 생성 기법과 모델 편집 기능 평가

김한결 ( Han-gyeol Kim ),임평채 ( Pyung-chae Lim ),황윤혁 ( Yunhyuk Hwang ),김동하 ( Dong Ha Kim ),김태정 ( Taejung Kim ),이수암 ( Sooahm Rhee ) 대한원격탐사학회 2021 大韓遠隔探査學會誌 Vol.37 No.6

본 논문에서는 포인트 클라우드 데이터를 기반으로 자동으로 군집형 솔리드 건물 모델을 생성하는 기술을 다양한 데이터에 적용, 실험을 수행하여 적용 가능성과 효용성을 탐색한다. 또한 자동 건물 모델링 기술의 한계로 인해 부족한 모델의 품질을 향상시키기 위하여 건물의 형상 편집 및 텍스처 조정 기술을 개발, 실험을 통해 결과를 확인하였다. 자동 건물 모델 생성 기술의 적용 가능성 탐색을 위하여 무인항공기 영상 기반으로 생성된 포인트 클라우드와 LiDAR(Light Detection and Ranging) 자료를 사용해 실험하였으며, 자동으로 생성된 건물 모델에 건물 형상 편집 및 텍스처 조정 기술을 적용하여 모델의 품질의 향상 실험을 수행하였다. 이를 통해 포인트 클라우드 데이터 기반의 자동 군집형 솔리드 건물 모델링 기술의 적용 가능성과 모델의 품질 향상 기술의 효용성을 확인하였다. 개발된 기술은 기존의 건물 모델링 기술과 비교하여 처리시간의 비용이 크게 감소하며, 잦은 모델 갱신이 필요한 지역에 대한 관리 측면에서도 강점이 있을 것으로 기대된다. In this paper, we explore the applicability and utility of a technology that generating clustered solid building models based on point cloud automatically by applying it to various data. In order to improve the quality of the model of insufficient quality due to the limitations of the automatic building modeling technology, we develop the building shape modification and texture correction technology and confirmed the results through experiments. In order to explore the applicability of automatic building model generation technology, we experimented using point cloud and LiDAR (Light Detection and Ranging) data generated based on UAV, and applied building shape modification and texture correction technology to the automatically generated building model. Then, experiments were performed to improve the quality of the model. Through this, the applicability of the point cloud data-based automatic clustered solid building model generation technology and the effectiveness of the model quality improvement technology were confirmed. Compared to the existing building modeling technology, our technology greatly reduces costs such as manpower and time and is expected to have strengths in the management of modeling results.

무인항공기 정밀 센서모델링을 통한 대축척 수치도화 가능성 평가

임평채 ( Pyung-chae Lim ),김한결 ( Han-gyeol Kim ),박지민 ( Jimin Park ),이수암 ( Sooahm Rhee ) 대한원격탐사학회 2020 大韓遠隔探査學會誌 Vol.36 No.6

무인항공기(UAV: Unmanned Aerial Vehicle)는 저고도 비행으로 인해 고해상도 영상을 취득할 수 있으며, 수시촬영이 가능하여 지도제작에 있어 수시갱신이 가능하다. 이러한 이점으로 인해 무인항공기 영상을 이용한 대축척 수치지도 제작 가능성에 대한 연구가 활발히 진행되고 있다. 정밀한 수치지도는 디지털트윈이나 스마트시티의 기반 데이터로 활용될 수 있다. 정밀한 수치지도를 제작하기 위해서는 지상기준점을 이용한 정밀 센서모델링이 반드시 선행되어야 한다. 본 연구에서는, 자체 개발한 정밀 센서모델링 알고리즘을 통해 무인항공기 영상의 기하모델을 수립하였다. 그리고 수치지도를 제작하여 대축척 수치도화 가능성을 평가하였다. 연구 데이터는 인천 간석동과 서울 여의도를 대상으로 영상 및 지상기준점을 취득하였다. 정밀 센서모델링 정확도 분석 결과, 두 지역에 대해서 체크 점 평균오차 3 픽셀 이내, RMSE 4 픽셀 이내의 높은 정확도를 확인하였다. 수치도화 정확도 분석 결과, 국토지리정보원에서 고시한 1:1,000 세부도화 수평오차(0.4 m) 및 표고오차(0.4 m)를 만족하는 범위의 정확도를 확인하였다. 따라서 본 연구에서 자체개발한 정밀 센서모델링 기술은 무인항공기 영상의 1:1,000 대축척 수치도화 제작 가능성을 시사한다. UAV (Unmanned Aerial Vehicle) can acquire high-resolution images due to low-altitude flight, and it can be photographed at any time. Therefore, the UAV images can be updated at any time in map production. Due to these advantages, studies on the possibility of producing large-scale digital maps using UAV images are actively being conducted. Precise digital maps can be used as base data for digital twins or smart cites. For producing a precise digital map, precise sensor modeling using GCPs (Ground Control Points) must be preceded. In this study, geometric models of UAV images were established through a precision sensor modeling algorithm developed in house. Then, a digital map by stereo plotting was produced to evaluate the possibility of large-scale digital map. For this study, images and GCPs were acquired for Ganseok-dong, Incheon and Yeouido, Seoul. As a result of precision sensor modeling accuracy analysis, high accuracy was confirmed within 3 pixels of the average error of the checkpoints and 4 pixels of the RMSE was confirmed for the two study regions. As a result of the mapping accuracy analysis, it satisfied the 1:1,000 mapping accuracy announced by the NGII (National Geographic information Institute). Therefore, the precision sensor modeling technology suggested the possibility of producing a 1:1,000 large-scale digital map by UAV images.

The Adenylyl Cyclase Encoded by rutabaga Is Required for Larval Molting in Drosophila

Son, Wonseok,Han, Pyung-Lim,Chung, Yun Doo Korean Society of Genetics 2008 Genes & Genomics Vol.30 No.2

Hormonal control of insect growth and metamorphosis by neuroendocrine system is well established, yet the cellular mechanism underlying the synthesis and release of insect hormones is not clearly understood. Here we report that a novel enhancer detection line, rut<SUP>lethal-1096</SUP>, failed to molt from second to third instar larvae, thus showing a lethal phenotype. Molecular cloning indicated that the enhancer detector was inserted in the proximal promoter region of the rut-encoded adenylyl cyclase (AC) gene. Similarly, the known rut alleles, rut<SUP>1</SUP> and rut<SUP>1084</SUP>, were also found to be affected mildly in their larval and pupal development. The enriched expression of the reporter gene lacZ was detected in the ring gland that is an insect larval glandular organ secreting various hormones involved in ecdysis. Taken together, our data suggest the rut-encoded AC plays a vital role in larval molting, probably by regulating the function of ring glands.

Activation of Autophagy Pathway Suppresses the Expression of iNOS, IL6 and Cell Death of LPS-Stimulated Microglia Cells

( Hye Eun Han ),( Tae Kyung Kim ),( Hyung Jin Son ),( Woo Jin Park ),( Pyung Lim Han ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.1

Microglia play a role in maintaining and resolving brain tissue homeostasis. In pathological conditions, microglia release pro-inflammatory cytokines and cytotoxic factors, which aggravate the progression of neurodegenerative diseases. Autophagy pathway might be involved in the production of pro-inflammatory cytokines and cytotoxic factors in microglia, though details of the mechanism remain largely unknown. In the present study, we examined the role of the autophagy pathway in activated BV2 microglia cells. In BV2 cells, rapamycin treatment activated the formation of anti-LC3-labeled autophagosomes, whereas the ATG5 depletion using siRNA-ATG5 prevented the formation of LC3-labeled autophagosomes, indicating that BV2 cells exhibit an active classical autophagy system. When treated with LPS, BV2 cells expressed an increase of anti-LC3-labeled dots. The levels of LC3-labeled dots were not suppressed, instead tended to be enhanced, by the inhibition of the autophagy pathway with siRNA-ATG5 or wortmannin, suggesting that LPS-induced LC3-labeled dots in nature were distinct from the typical autophagosomes. The levels of LPS-induced expression of iNOS and IL6 were suppressed by treatment with rapamycin, and conversely, their expressions were enhanced by siRNA-ATG5 treatment. Moreover, the activation of the autophagy pathway using rapamycin inhibited cell death of LPS-stimulated microglia. These results suggest that although microglia possess a typical autophagy pathway, the glial cells express a non-typical autophagy pathway in response to LPS, and the activation of the autophagy pathway suppresses the expression of iNOS and IL6, and the cell death of LPS-stimulated microglia.

Mice lacking adenylyl cyclase-5 cope badly with repeated restraint stress

Kim, Kyoung-Shim,Han, Pyung-Lim Wiley Subscription Services, Inc., A Wiley Company 2009 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.87 No.13

<P>Physiological responses to acute stress proceed with the activation of the hypothalamus-pituitary-adrenal gland (HPA) system. Many brain regions are known to modulate the HPA axis activation in stress responses, but the detailed neural circuits and signaling system in the upstream of the HPA axis have to be explored further. Type 5 adenylyl cyclase (AC5) is highly concentrated in the dorsal striatum and nucleus accumbens, which are implicated in reward and stress-related behavior. AC5<SUP>–/–</SUP> mice exposed to daily 2-hr restraint stress for only 3–5 days showed poor stress-coping responses, including severe body weight loss, poor coat condition, respiratory difficulties, and freezing behavior. Plasma corticosterone levels during 2-hr stress sessions increased in AC5<SUP>–/–</SUP> mice compared with those of AC5<SUP>+/+</SUP> mice. However, neither the corticosterone receptor antagonist RU486 nor the CRH receptor antagonist NBI27914 blocked their poor stress coping, whereas the administration of the GABA<SUB>A</SUB> receptor allosteric modulator diazepam or the D1 dopamine receptor antagonist SCH23390 prior to restraint stress sessions changed their stress-coping response to the stressed AC5<SUP>+/+</SUP> mouse level. Stress-triggered c-Fos expression was completely blunted in the dorsal striatum of AC5<SUP>–/–</SUP>. These results suggest that the AC5-associated signal system and neural network are involved in the regulation of anxiety and stress-coping response. © 2009 Wiley-Liss, Inc.</P>

하드웨어를 이용한 3D 실시간 카툰렌더링 설계

한득수 ( Deuk-su Han ),김광민 ( Kwang-min Kim ),임평종 ( Pyung-jong Lim ),곽훈성 ( Hoon-sung Kwak ) 한국정보처리학회 2006 한국정보처리학회 학술대회논문집 Vol.13 No.2

본 연구는 디지털 문화의 출현이라는 맥락에서 디지털 시대의 환경 변화를 맞이한 애니메이션 제작 기법의 변화 속에서 셀 애니메이션 작품과 혼합하여 사용한 3D 컴퓨터 그래픽스(3D Computer Graphics) 기법 중 비사실적 이미지 기법(Non Photo Realistic Rendering)의 범주에 속한 카툰 렌더링(Cartoon Rendering) 기법을 분석하고 그것이 갖는 PC기반 실시간으로 수행되는 비사실적 렌더링을 위한 실시간 카툰렌더링 기법을 제시하고 있다. 종래의 카툰렌더링에 대해 연구하고 기존의 비사실적 이미지 기법의 적용에 대해 알아본다.