FET형 반도체 마이크로센서 개발(Ⅲ)

손병기,이흥락,박이순,조진호,이성필,최평,서화일,박재윤,송경은,김창수 경북대학교 센서기술연구소 1995 연차보고서 Vol.1995 No.-

현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte : H^+, K^+, Ca^2+, Na^+)센서소자 및 분석 시스템, 용존가스(O_2, CO_2)센서의 개발을 중점적으로 추진하였으며, FET형 압력센서, 습도센서 둥의 개별 FET형 센서에 관한 기초연구도 병행하였다. The conventional sensors have many problems such as high cost, large dimension, long analysis time and troublesome handling to apply to the fields of medical diagnosis, monitoring of chemical process and environmental monitoring/control. The main objects of this research are to develope a new FET type electrolyte(H^+, K^+, Ca^2+, Na^+)sensors, analysis system, and dissoved gas(O_2, CO_2)senors that can overcome the problems of the conventional sensors, and parallel basic researches on FET. type sensors such as pressure and humidity are also in progress.

Tuberculosis and Respiratory Diseases : The expression of Notch1 during eosinophil differentiation

( Yun Hwa Hong ),( Hun Soo Chang ),( Jin Hyun Kang ),( Seung Ha Lee ),( Pyung Oh Yoon ),( Soo Ho Kim ),( Eun Young Kim ),( Hwan Man Shin ),( Tae Yun Lim ),( Il Yup Chung ),( Choon Sik Park ) 대한결핵 및 호흡기학회 2002 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.95 No.-

Biological Functions and Identification of Novel Biomarker Expressed on the Surface of Breast Cancer-Derived Cancer Stem Cells via Proteomic Analysis

Koh, Eun-Young,You, Ji-Eun,Jung, Se-Hwa,Kim, Pyung-Hwan Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.4

Breast cancer is one of the most common life-threatening malignancies and the top cause of cancer deaths in women. Although many conventional therapies exist for its treatment, breast cancer still has many handicaps to overcome. Cancer stem cells (CSCs) are a well-known cause of tumor recurrences due to the ability of CSCs for self-renewal and differentiation into cell subpopulations, similar to stem cells. To fully treat breast cancer, a strategy for the treatment of both cancer cells and CSCs is required. However, current strategies for the eradication of CSCs are non-specific and have low efficacy. Therefore, surface biomarkers to selectively treat CSCs need to be developed. Here, 34 out of 641 surface biomarkers on CSCs were identified by proteomic analysis between the human breast adenocarcinoma cell line MCF-7 and MCF-7-derived CSCs. Among them, carcinoembryonic antigen-related cell adhesion molecules 6 (CEACAM6 or CD66c), a member of the CEA family, was selected as a novel biomarker on the CSC surface. This biomarker was then experimentally validated and evaluated for use as a CSC-specific marker. Its biological effects were assessed by treating breast cancer stem cells (BCSCs) with short hairpin (sh)-RNA under oxidative cellular conditions. This study is the first to evaluate the biological function of CD66c as a novel biomarker on the surface of CSCs. This marker is available as a moiety for use in the development of targeted therapeutic agents against CSCs.

新生兒의 血淸 Alpha-Fetoprotein値에 對하여

李圭殷,崔平和 고려대학교 의과대학 1980 고려대 의대 잡지 Vol.17 No.1

In order to evaluate the role of AFP in the estimation of gestational age, AFP in cord sera was determined by radioimmunoassay (PEG method) from 117 newborn infants at various gestational age of whom pregnant women known their exact. LMP were delivered. Cord blood samples were collected from newborn infants immediately after delivery. The results obtained were as follows: 1. The majority of maternal age were in age group of 25-29 years, 64 cases (54.7%) 2. Among the total number of 117 cases 64 cases (54.7%) were male and 53 cases (45.3%) female. The gestational ages were distributed from 28 weeks to 44 weeks. The majority were in gestational age of 39-40 weeks, 37 cases (31.6%). Among 117 cases studies, 63 cases (53.9%) were fullterm infants, 43 cases (36.7%) premature infants and 11 cases (9.4%) postterm infants. 3. Birthweight of newborn infants was in a range of 1300 to 4100gm. The most frequent birth weight of newborn infants was ranged from 3001 to 3500gm, 42 cases (35.9%). 4. The maximum level of AFP in cord serum determined in the present study was 45.0㎎/㎗ and the minimum level 3.2㎎/㎗. The average maximal serum AFP levels were 35.75±13.06㎎/㎗ in the gestational age of less than 30 weeks, and thereafter AFP levels decreased with increasing gestational age. The average levels of serum AFP between 39 and 40 weeks of gestation were 5.84±2.68㎎/㎗. 5. There was a close negative correlation between AFP in cord serum and gestational age (r= -0.645, p<0.001) and the regression line can be derived from the formula y= -0.28x+88.47. 6. Also there was a negative correlation between AFP in cord serum and birth weight (r= -0.6085, p<0.001). This was less pronounced than for AFP in cord serum versus gestational age. The regression equation was y= -8.975x+36.441. 7. The AFP levels were significantly higher in preterm AGA infant than those in term AGA. However there were no significant differences between term SGA and term AGA. 8. The coefficient of correlation between AFP in cord serum and gestational age was slightly higher than that between AFP and birthweight. In this point of the above results, the further investigation should be carried out for exact assessment of gestational age by means of AFP in cord blood.

The Effect of Annexin A1 as a Potential New Therapeutic Target on Neuronal Damage by Activated Microglia

You, Ji-Eun,Jung, Se-Hwa,Kim, Pyung-Hwan Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.4

Brain disease is known to cause irrevocable and fatal loss of biological function once damaged. One of various causes of its development is damage to neuron cells caused by hyperactivated microglia, which function as immune cells in brain. Among the genes expressed in microglia stimulated by various antigens, annexin A1 (ANXA1) is expressed in the early phase of the inflammatory response and plays an important role in controlling the immune response. In this study, we assessed whether ANXA1 can be a therapeutic target gene for the initial reduction of the immune response induced by microglia to minimize neuronal damage. To address this, mouse-origin microglial cells were stimulated to mimic an immune response by lipopolysaccharide (LPS) treatment. The LPS treatment caused activation of ANXA1 gene and expression of inflammatory cytokines. To assess the biological function in microglia by the downregulation of ANXA1 gene, cells were treated with short hairpin RNA-ANXA1. Downregulated ANXA1 affected the function of mitochondria in the microglia and showed reduced neuronal damage when compared to the control group in the co-culture system. Taken together, our results showed that ANXA1 could be used as a potential therapeutic target for inflammation-related neurodegenerative diseases.

Do Severity Score and Skin Temperature Asymmetry Correlate with the Subjective Pain Score in the Patients with Complex Regional Pain Syndrome?

Jeon, Seung Gyu,Choi, Eun Joo,Lee, Pyung Bok,Lee, Young Jae,Kim, Min Soo,Seo, Joung Hwa,Nahm, Francis Sahngun The Korean Pain Society 2014 The Korean Journal of Pain Vol.27 No.4

Background: The diagnostic criteria of complex regional pain syndrome (CRPS) have mainly focused on dichotomous (yes/no) categorization, which makes it difficult to compare the inter-patient's condition and to evaluate the intra-patient's subtle severity over the course of time. To overcome this limitation, many efforts have been made to create laboratory methods or scoring systems to reflect the severity of CRPS; measurement of the skin temperature asymmetry is one of the former, and the CRPS severity score (CSS) is one of the latter. However, there has been no study on the correlations among the CSS, temperature asymmetry and subjective pain score. The purpose of this study was to evaluate whether there is any correlation between the CSS, skin temperature asymmetry and subjective pain score. Methods: Patients affected with CRPS in a unilateral limb were included in this study. After making a diagnosis of CRPS according to the Budapest criteria, the CSS and skin temperature difference between the affected and unaffected limb (${\Delta}T$) was measured in each patient. Finally, we conducted a correlation analysis among the CSS, ${\Delta}T$ and visual analogue scale (VAS) score of the patients. Results: A total of 42 patients were included in this study. There was no significant correlation between the ${\Delta}T$ and VAS score (Spearman's rho = 0.066, P = 0.677). Also, the CSS and VAS score showed no significant correlation (Spearman's rho = 0.163, P = 0.303). Conclusions: The ${\Delta}T$ and CSS do not seem to reflect the degree of subjective pain in CRPS patients.

Lactobacillus-derived extracellular vesicles counteract Aβ42-induced abnormal transcriptional changes through the upregulation of MeCP2 and Sirt1 and improve Aβ pathology in Tg-APP/PS1 mice

Kwon Hyejin,Lee Eun-Hwa,Park So Young,Park Jin-Young,Hong Jin-Hwan,Kim Eun-Kyung,Shin Tae-Seop,김윤근,Han Pyung-Lim 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Mounting evidence suggests that probiotics are beneficial for treating Alzheimer’s disease (AD). However, the mechanisms by which specific probiotics modify AD pathophysiology are not clearly understood. In this study, we investigated whether Lactobacillus paracasei-derived extracellular vesicles (Lpc-EV) can directly act on neuronal cells to modify amyloid-beta (Aβ)-induced transcriptional changes and Aβ pathology in the brains of Tg-APP/PS1 mice. Lpc-EV treatment in HT22 neuronal cells counteracts Aβ-induced downregulation of Brain-derived neurotrophic factor (Bdnf), Neurotrophin 3 (Nt3), Nt4/5, and TrkB receptor, and reverses Aβ-induced altered expression of diverse nuclear factors, including the downregulation of Methyl-CpG binding protein 2 (Mecp2) and Sirtuin 1 (Sirt1). Systematic siRNA-mediated knockdown experiments indicate that the upregulation of Bdnf, Nt3, Nt4/5, and TrkB by Lpc-EV is mediated via multiple epigenetic factors whose activation converges on Mecp2 and Sirt1. In addition, Lpc-EV reverses Aβ-induced downregulation of the Aβ-degrading proteases Matrix metalloproteinase 2 (Mmp-2), Mmp-9, and Neprilysin (Nep), whose upregulation is also controlled by MeCP2 and Sirt1. Lpc-EV treatment restores the downregulated expression of Bdnf, Nt4/5, TrkB, Mmp-2, Mmp-9, and Nep; induces the upregulation of MeCP2 and Sirt1 in the hippocampus; alleviates Aβ accumulation and neuroinflammatory responses in the brain; and mitigates cognitive decline in Tg-APP/PS1 mice. These results suggest that Lpc-EV cargo contains a neuroactive component that upregulates the expression of neurotrophic factors and Aβ-degrading proteases (Mmp-2, Mmp-9, and Nep) through the upregulation of MeCP2 and Sirt1, and ameliorates Aβ pathology and cognitive deficits in Tg-APP/PS1 mice.

Do Severity Score and Skin Temperature Asymmetry Correlate with the Subjective Pain Score in the Patients with Complex Regional Pain Syndrome?

( Seung Gyu Jeon ),( Eun Joo Choi ),( Pyung Bok Lee ),( Young Jae Lee ),( Min Soo Kim ),( Joung Hwa Seo ),( Francis Sahngun Nahm ) 대한통증학회 2014 The Korean Journal of Pain Vol.27 No.4

Background:The diagnostic criteria of complex regional pain syndrome (CRPS) have mainly focused on dichotomous (yes/no) categorization, which makes it difficult to compare the inter-patient`s condition and to evaluate the intra-patient``s subtle severity over the course of time. To overcome this limitation, many efforts have been made to create laboratory methods or scoring systems to reflect the severity of CRPS; measurement of the skin temperature asymmetry is one of the former, and the CRPS severity score (CSS) is one of the latter. However, there has been no study on the correlations among the CSS, temperature asymmetry and subjective pain score. The purpose of this study was to evaluate whether there is any correlation between the CSS, skin temperature asymmetry and subjective pain score.Methods:Patients affected with CRPS in a unilateral limb were included in this study. After making a diagnosis of CRPS according to the Budapest criteria, the CSS and skin temperature difference between the affected and unaffected limb (ΔT) was measured in each patient. Finally, we conducted a correlation analysis among the CSS, ΔT and visual analogue scale (VAS) score of the patients.Results:A total of 42 patients were included in this study. There was no significant correlation between the ΔT and VAS score (Spearman`s rho = 0.066, P = 0.677). Also, the CSS and VAS score showed no significant correlation (Spearman`s rho = 0.163, P = 0.303).Conclusions:The ΔT and CSS do not seem to reflect the degree of subjective pain in CRPS patients. (Korean J Pain 2014; 27: 339-344)

국내 주요 국가산업단지에서 중금속에 의한 토양오염

정태욱 ( Tae Uk Jeong ),조은정 ( Eun Jeong Cho ),정재은 ( Jae Eun Jeong ),지화성 ( Hwa Seong Ji ),이경심 ( Kyeong Sim Lee ),유평종 ( Pyung Jong Yoo ),김기곤 ( Gi Gon Kim ),최지연 ( Ji Yeon Choi ),박종환 ( Jong Hwan Park ),김성헌 ( 한국환경농학회 2015 한국환경농학회지 Vol.34 No.2

BACKGROUND: Contamination of soils by heavy metals is the serious environmental problem. In particular, industrial processing is one of the main sources of heavy metal contamination. The objective of this study was to investigate the distribution characteristics of heavy metals in soils collected from industrial complex. METHODS AND RESULTS: In this study, the soil contamination and enrichment factor (EF) of heavy metals were investigated in three national industrial complexes such as Yeosu, Ulsan and Sihwa·Banwal industrial complexes. The target heavy metals includes Cd, Cu, As, Hg, Pb, Cr, Zn, and Ni. The results showed that the contents of Cd, Hg, Pb, Zn and Ni in Yeosu and the contents of Cu, As and Cr in Sihwa·Banwal were higher than in any other industrial complex. The results of principal component analysis(PCA) in Yeosu, Ulsan and Sihwa·Banwal complex could be explained up to approximately 81.4, 69.1 and 70.9% by two factor, respectively. Enrichment factors of Cd, Pb and Zn in all the investigated industrial complexes were above 1.0 that was the value judged to be a high contamination. And EF of Cr was above 1.0 in Sihwa·Banwal complex. EF of Zn in all sites was generally high from the other heavy metals. CONCLUSION: Therefore, soils maybe significantly affected by heavy metals (especially, Cd, Pb and Zn) present in the emissions from industrial complexes.

CT sinogram‐consistency learning for metal‐induced beam hardening correction

Park, Hyoung Suk,Lee, Sung Min,Kim, Hwa Pyung,Seo, Jin Keun,Chung, Yong Eun Published for the American Association of Physicis 2018 Medical physics Vol.45 No.12