Studies on the prevalence of HIV/AIDS in Bangladesh including other developing countries

Rahman Md. Mominur,Fahadul Islam,Rahaman Md. Saidur,Nazneen Ahmeda Sultana,Fyrose Fahim Nahian,Muniruddin Ahmed 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.3

The outbreak of HIV/AIDS is not yet high in Bangladesh but slowly increasing posing intimidation. The process of this review was carried out to explore the danger of HIV/AIDS to acquaint knowledge about prevention and transmission of HIV, attitudes unto HIV prevention and behaviors utter to HIV/AIDS. As an intervention strategy, provided training and awareness can ensure that the people of a society or country become a source of the correct information and well-educated persons with whom young generation can share sensitive and complicated issues about sexuality. Peer-reviewed articles were identified to use a systematic way to collect databases. A strategy was designed to identify the articles for publishing in the high ranked journals. All the search results from the prominent databases, named Elsevier, Scopus, Springer, PubMed, and Cochrane, were collected as well as all possible copied were abandoned. This article emphasis shed light to government policymakers, NGOs and other social institutions, also inspire them to take special attention to minimize sexual discrimination, sexual misuse and ferocity prior implementing any insurance for the prevention of HIV/AIDS in Bangladesh and in developing countries. This study analyzed how girls and women in Bangladesh including developing nations marginalized and victim to HIV/AIDS due to gender discrimination, lack of proper knowledge and awareness and other relevant issues.

Antioxidants Show a Protective Effects against Bisphenol A Induced Stress on Spermatozoa

Md Saidur Rahman,Kyu-Ho Kang,Sarder Arifuzzaman,Won-Ki Pang,Do-Yeal Ryu,Won-Hee Song,Myung-Geol Pang 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Bisphenol A (BPA), an endocrine-disrupting chemical, has received tremendous attention in the past few decades because of its detrimental health effects. Growing evidence supports that BPA is capable to alter the reproductive performance of the exposed individual. In spermatozoa, it has been reported that BPA increased oxidative stress by the overproduction of reactive oxygen species (ROS), subsequently affects the sperm function, biochemical properties, and fertility. Since antioxidants minimize cellular oxidative stress, therefore may have protective effects against BPA-induced stress. In the present study, we incubated mice spermatozoa for 6 h in a condition that support in vitro fertilization. The sperm incubation media was additionally supplemented with either BPA or BPA together with antioxidants, such as glutathione, vitamin C, and vitamin E. Our results showed that antioxidant significantly decreased the production of ROS that subsequently supports motility and acrosomal integrity of BPA-exposed spermatozoa. Particularly, glutathione and vitamin E inhibit protein kinase-A dependent phosphorylation of sperm proteins subsequently prevented precocious acrosome reaction. In addition, both antioxidants were found to restore fertilization and early embryo development potentiality of BPA-exposed spermatozoa. Therefore, we conclude that antioxidants minimize oxidative stress in spermatozoa in a BPA containing micro-environment, thus avoiding BPA-mediated harmful consequences. The current finding has both theoretical and clinical significance for developing potential remedies of the BPA toxicity.

Alterations of Male Fertility following Exposure to Bisphenol A

Md Saidur Rahman 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Bisphenol A (BPA) is a common industrial chemical that has been used extensively to make certain plastics and resins since the 1960s. As a potential endocrine disruptors, BPA has been investigated for its impact on fertility/reproduction in animals and humans. However, the molecular mechanisms of BPA action and standard method for detecting BPA-related health hazards are unclear. Considering in-vitro experimental model, we investigated the effects of BPA (0.0001 to 100 μM) exposure on mouse spermatozoa. We revealed that BPA affects several sperm functions by triggering the mitogen-activated protein kinase, phosphatidylinositol 3-kinase, and protein kinase-A (PKA) activity. High doses of this chemical was also likely for the activation of protein tyrosine phosphorylation in a PKA-dependent signaling consequently induced a precocious acrosome reaction. Simultaneously, BPA has been found to decrease the rate of fertilization and early embryonic development. In addition, BPA induced differential protein expression in spermatozoa were responsible for the pathogenesis of many diseases. Considering in vivo experimental model, we deliberate the effects of gestational BPA exposure (TDI, NOAEL, and LOAEL doses) on both ejaculated and capacitated spermatozoa in F1 adult mice. We confirmed that BPA affects several sperm function in F1 male. These effects appeared to be caused by reduced numbers of stage VIII seminiferous epithelial cells in testis and decreased PKA activity and tyrosine phosphorylation (non-capacitated) in spermatozoa. We also noticed that BPA decreased average litter size as well as compromise the rates of cleavage and blastocyst formation. Proteins differentially expressed in both capacitated/ejaculated spermatozoa play a critical role in energy metabolism, stress responses, and fertility, finally predispose to the development of several diseases. On the basis of these results, we suggest that BPA alter spermatozoa function and the proteomic profile, ultimately affecting their fertility potential. Therefore, it is of critical public health significance to reevaluate the levels of BPA exposure that are currently deemed to be acceptable.

Bioinformatics Annotation of Human X and Y Chromosome-Encoded Proteins associated to Immune Functions

Md Saidur Rahman,Woo-Sung Kwon,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

Even human genome has been sequenced completely, we have very limited idea of all characterized genes at the protein level. Therefore, several attempts have been taken to map proteome of human chromosome. Since sex chromosomes determined the sex of individual, it important to study sex chromosome-encoded proteins. Here, we studied human sex chromosome- encoded proteins of the immune system, protein pathways, protein-protein interactions, and diseases association using several bioinformatics tools. We retrieved 30 proteins (X chromosome 28; Y chromosome 7; both 5) from the recent NCBI human genome annotation, based on their association with immune systems (immunological and inflammation pathways) by Pathway studio program. Searching of these proteins in the Human Proteome Project, including neXtProt, PeptideAtlas, and the Human Protein Atlas showed that all proteins were also identified in several cells and tissues of body. Proteins were further investigated using Pathway Studio and STRING programs. Pathway Studio and STRING programs showed 15 and 25 X chromosome-encoded proteins were interacted. All Y chromosome-encoded proteins showed interactions using STRING program, however no interaction was found using Pathway studio. In addition, difference in human sex chromosome-encoded proteins of the immune system showed an indirect relationship with the occurrence of some diseases in a sex specific manner.

Duration of Preservation Affect the Quality of Chilled Black Bengal Buck Semen

Md Gulshan Anowar Pradhan,Md Saidur Rahman,Woo-Sung Kwon,Dipendra Mishra,Md Mostofa Kamal,Mohammad Musharraf Uddin Bhuiyan,Mohammed Shamsuddin 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.2

The study focuses on the quality assessment of Black Bengal buck semen preserved at chilled condition. In this in vitro trial, collected semen from Black Bengal bucks was preserved at chilling temperature (4▲5줛) in tris-glucosecitrate yolk medium of 1:5 ratios for four days. Artificial Vagina (AV) method was utilized to collect semen from buck. General evaluation of semen includes the color, mass activity and density were measured by direct visual examination. However, computer-assisted sperm analysis (CASA) and phase contrast microscopy were used to figure out the motility (%), hyper-activated (HYP) motility (%) and number of abnormal spermatozoa (%) initially, and at every 24 h intervals. The result revealed that spermatozoa preserved at chilling temperature showed significantly (P<0.05) lower motility and HYP motility with the progression of preservation. The number of phenotypically abnormal spermatozoa significantly (P<0.05) increased following preservation. Although significant positive correlation (r=0.945; P<0.05) was existed between % motile and % HYP motile spermatozoa however, the % of morphologically abnormal spermatozoa was negatively correlated with % motile (r=긏0.997; P<0.05) and % HYP motile spermatozoa (r=긏0.946; P<0.01). Therefore, we concluded that the quality of chilled semen progressively losses its viability and doesn…t remain useable after certain period of preservation with respect to its motility and morphology.

The Transgenerational Significance of Bisphenol‒A on Sperm Function and Fertility of CD-1 Male Mice

Md Saidur Rahman,Won-Ki Pang,Ki-Uk Kim,Ye-Ji Kim,Do-Yeal Ryu,Amena Khatun,Sung-Jae Yoon,Woo-Sung Kwon,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

Sodium Nitroprusside Induce Phosphorylation of Ferritin Light Chain Protein, Regulates the Acrosome Reaction and Fertility of Mice Spermatozoa

Md Saidur Rahman,Won-Ki Pang,Ye-Ji Kim,Do-Yeol Ryu,Amena Khatun,Sung-Jae Yoon,Woo-Sung Kwon,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2015 발생공학 국제심포지엄 및 학술대회 Vol.2015 No.10

Perinatal exposure of female mice to bisphenol-A affects the sperm functions and fertilization of male offspring

Md Saidur Rahman,Woo-Sung Kwon,Ye-Ji Kim,Do-Yeal Ryu,Hyun-Gu Kang,Amena Khatun,Sung-Jae Yoon,Myung-Geol Pang 한국발생생물학회 2015 한국발생생물학회 학술발표대회 Vol.2015 No.9

As an endocrine disruptor, bisphenol-A (BPA) causes several functional and behavioral abnormalities related to reproduction. The current study was design to evaluate the effect of perinatal exposure of female mice to BPA on sperm function of adult F(1) offspring. Pregnant female mice F(0) were gavaged with three different concentration of BPA, such as 50 μg/kg/day (tolerable daily intake value by the European Food Safety Authority), 5 mg/kg/day (no-observed-adverse-effect level; NOAEL), and 50 mg/kg/day (lowest-observed-adverse-effect level; LOAEL) and corn oil (7 mg/kg/day; vehicle control). The functional parameters of F(1) spermatozoa were studied both before and after capacitation, whereas the fertility assessment was evaluated by in vitro and in vivo assay using unexposed females. Our results showed that spermatozoa hyperactivated motility, capacitation, intracellular ATP, Ca2+, and ROS levels after capacitation were significantly affected using NOAEL and LOAEL concentration of BPA. However, the sperm motility was only affected by LOAEL dose after capacitation. All of the tested parameters were potentially unaffected by BPA before capacitation, except intracellular ATP that decreased by all concentrations. Although both NOAEL and LOAEL concentration were effectively reduced the rate of fertilization and embryonic development in vitro, however the average litter size was only affected by LOAEL dose. Our finding suggested that perinatal exposure of 50 μg/kg/day did not produce significant effects; however both NOAEL and LOAEL affects overall sperm function after capacitation, leading to impairments in the fertility of F(1) male offspring.

A novel approach to assessing bisphenol-A hazard using in vitro model system

Md Saidur Rahman,Woo-Sung Kwon,Ye-Ji Kim,Do-Yeal Ryu,Amena Khatun,Sung-Jae Yoon,Myung-Geol Pang 한국발생생물학회 2015 한국발생생물학회 학술발표대회 Vol.2015 No.9

Although the toxicological impacts of the xenoestrogen bisphenol-A (BPA) have been studied extensively, but its mechanism of action is poorly understood. Eventually, no standard method exists for evaluating the possible health hazard of BPA. Considering mice spermatozoa as a potential in vitro model, here we demonstrated the effects of BPA exposure (0.0001, 0.01, 1, and 100 μM for 6 h) on spermatozoa and the related mechanisms of action. Our results demonstrated that high concentrations of BPA negatively affect sperm motility, viability, intracellular ATP, and mitochondrial functions by activating the mitogen-activated protein kinase, phosphatidylinositol 3-kinase, and protein kinase-A pathways. The same doses were also employed to identify the differential expressed proteins of exposure and screen their functional affiliation to diseases using sperm proteomics and informatics, respectively. Our results demonstrated that a high concentration of BPA (100 μM) induced differential expression (> 2-fold) of 24 proteins in spermatozoa (16 down- and 9 up-regulated), that are putatively involved in the pathogenesis of several diseases. To the best of our knowledge, this is the first study to demonstrate the mechanisms of BPA action in spermatozoa and to identify the possible biomarkers of exposure. Moreover, we anticipated that current strategy might apply for the hazard assessment of other toxicological agents.

Application of Ultrasonography to Investigate Postpartum Anestrus in Water Buffaloes

Rahman, Md Saidur,Shohag, Abu Said,Kamal, Md. Mostafa,Parveen, Nasreen,Shamsuddin, Mohammed The Korean Society of Animal Reproduction 2012 Reproductive & developmental biology Vol.36 No.2

Anestrus is one of the most important production limiting disorders in dairy buffaloes and its underlying causes have been a current topic of studies. The objectives of this study were to explore the causes of anestrus in buffaloes with the application of ultrasonography. Two examinations were performed by transrectal ultrasonography at 12 days apart in buffalo cows that were not seen in oestrus at 60 or more days postpartum. As high as 54.5% buffaloes had silent ovulation and 45.5% suffered from the true anestrus with ovarian dysfunction. The duration of anestrus after calving was 60~90, 91~120, 121~180 and 181~365 days in 27%, 32%, 18% and 23% buffalo cows, respectively. Treatment with prostaglandin of cyclic buffalo cows with a corpus luteum (72.7%) resulted in higher estrous rate as compared with close observation of estrus (23.1%) by the farmer (p=0.021). Acyclic buffalo cows without any corpus luteum on ovaries were successfully treated with gonadotropin releasing hormone (70%), resulting in higher estrous detection rate than those treated with a vitamin-mineral mixture (20%) (p=0.035). In conclusion, poor heat detection due to silent ovulation is the most important cause of apparent anoestrus in buffaloes; however the percentage of the true anestrous is also quite high in postpartum buffaloes.