The generalized Fermat type difference equations

Kai Liu,Lei Ma,Xiaoyang Zhai 대한수학회 2018 대한수학회보 Vol.55 No.6

This paper is to consider the generalized Fermat difference equations with different types which ever considered by Li \cite{li}, Ishizaki and Korhonen \cite{IK}, Zhang \cite{zhangjie} and Liu \cite{liuk4,11,1177,liu}, respectively. Some new observations and results on these equations will be given.

Sesquiterpenoids with Various Carbocyclic Skeletons from the Flowers of <i>Chrysanthemum indicum</i>

Liu, Lei-Lei,Ha, Thi Kim Quy,Ha, Wei,Oh, Won Keun,Yang, Jun-Li,Shi, Yan-Ping American Society of Pharmacognosy 2017 Journal of natural products Vol.80 No.2

<P>A phytochemical investigation of the flowers of Chrysanthemum indicum yielded sesquiterpenoids 1-25 with various carbocyclic skeletons, including 10 new (1-10) and 15 known (11-25) analogues. The structures were elucidated via their physical data, while the absolute configuration of compounds 6, 8, and 10 was assessed via electronic circular dichroism analysis. The evaluation of the effect of sesquiterpenoids on porcine epidemic diarrhea virus (PEDV) replication showed that compounds 1-5, 12, 14, 16, 17, 19, and 21 increased cell viability against cell death in PEDV-injected cells. Compounds 2, 12, and 17 were selected and investigated for their inhibition of proteins required for PEDV replication. Compounds 2 and 17 significantly reduced PEDV nucleocapsid and spike protein synthesis compared with azauridin as a positive control.</P>

MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

吴昌硕篆书创作小考

刘磊磊(Lei-Lei Liu),袁暁鹏(Xiao-Peng Yuan) 한국서예학회 2023 서예학연구 Vol.42 No.-

오창석(吴昌硕)의 전서는 필법, 구성, 장법, 묵법, 필법 등의 기법이 선인과 판이하게 다른데, 전서의 새로운 모습을 만들어내는 데 성공하여 큰 성과를 거둔 배경에는 여러 가지 이유가 있을 것이다. 우선 비파(碑派)서법의 서학사상을 흡수하여 금석(金石)을 취하고 고풍스럽고 웅장한 예술양식을 추앙하였다. 둘째, 등석여(邓石如), 양기손(杨沂孙) 등의 명가를 충분히 학습하는 기초 위에서 ≪석고문(石鼓文)≫을 주요 법으로 삼는 동시에 다양한 서체를 참고하여 여러 예술을 융합하고 자신의 예술 실천을 지속적으로 풍부하게 한다. 특히, 개인 특색 있는 필기구를 사용하여 개인 스타일을 지속적으로 강화합니다. 수십 년의 노력을 통해 마침내 ‘이리(二李)’ 전서 외에 다른 필법을 개척하고 전서 예술의 또 다른 정점을 창조하여 후대의 서예 예술 발전에 심대한 영향을 미쳤습니다. Wu Changshuo's seal book is very different from its predecessors in the use of pen, structure, sealing, ink, writing rhythm and other techniques, successfully shaping the new face of seal writing, and achieving great achievements, the reasons behind it should be many, first of all, he absorbed the calligraphy ideas of the stele calligraphy, took the golden stone, and advocated the simple and strong artistic style. Secondly, on the basis of fully learning from famous artists such as Deng Shiru(邓石如) and Yang Yisun(杨沂孙), he took “Shiguwen”(石鼓文) as the main object, and at the same time drew on a variety of book styles, integrated multiple arts, and constantly enriched his artistic practice. Third, use a very personal writing instrument to constantly strengthen your personal style. Through decades of hard work, it finally opened up another way outside the “Er Li” seal book, creating another peak of seal book art, which had a far-reaching impact on the development of calligraphy art in later generations. 吴昌硕的篆书在用笔、结构、章法、墨法、书写节奏等技法上都迥异于前人,成功地塑造了篆书的新面貌,取得了巨大的成就,其背后的原因应该是多方面的,首先,他吸收了碑派书法的书学思想,取法金石,推崇古朴雄强的艺术风格。其次,在充分学习邓石如、杨沂孙等名家的基础上,以≪石鼓文≫为主要取法对象,同时借鉴多种书体,融合多门艺术,不断丰富自己的艺术实践。再次,采用极具个人特色的书写工具,不断强化个人风格。通过几十年的努力,最终于“二李”篆书之外另辟蹊径,创造了篆书艺术的又一座高峰,对后世书法艺术的发展产生了深远的影响。

Prognostic Role of MicroRNA-21 in Non-small Cell Lung Cancer: a Meta-analysis

Ma, Xue-Lei,Liu, Lei,Liu, Xiao-Xiao,Li, Yun,Deng, Lei,Xiao, Zhi-Lan,Liu, Yan-Tong,Shi, Hua-Shan,Wei, Yu-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Introduction: Many studies have reported that microRNA-21 (miR-21) mihght predict the survival outcome in non-small cell lung cancers (NSCLCs) but the opposite opinion has also been expressed. The aim of this study was to summarize the evidence for a prognostic role of miR-21. Materials and Methods: All the eligible studies was searched by Medline and EMBASE and patients' clinical characteristics and survival outcome were extracted. Then a meta-analysis was performed to clarify the prognostic role of the miR-21 expression in different subgroups. Results: A total of 8 eligible articles were yielded covering survival outcomes or clinical characteristics. The combined hazard ratio (HR) and 95% confidence interval (95% CI) for overall survival (OS) was 2.19 [0.76, 6.30], while the combined HR (95% CI) of Asian group for OS had a significant result, 5.49 [2.46, 12.27]. The combined HR (95% CI) for recurrence free survival or disease free survival (RFS/DFS) was 2.31 [1.52, 3.49]. Odds ratios (ORs) showed that the miR-21 expression was associated with lymph node status and histological type. Conclusion: miR-21 expression could predict the prognostic outcome of NSCLC in Asians, despite some deficiencies in the study data.

Auto-Covariance Analysis for Depth Map Coding

( Lei Liu ),( Yao Zhao ),( Chunyu Lin ),( Huihui Bai ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.9

Efficient depth map coding is very crucial to the multi-view plus depth (MVD) format of 3-D video representation, as the quality of the synthesized virtual views highly depends on the accuracy of the depth map. Depth map contains smooth area within an object but distinct boundary, and these boundary areas affect the visual quality of synthesized views significantly. In this paper, we characterize the depth map by an auto-covariance analysis to show the locally anisotropic features of depth map. According to the characterization analysis, we propose an efficient depth map coding scheme, in which the directional discrete cosine transforms (DDCT) is adopted to substitute the conventional 2-D DCT to preserve the boundary information and thereby increase the quality of synthesized view. Experimental results show that the proposed scheme achieves better performance than that of conventional DCT with respect to the bitrate savings and rendering quality.

A clip domain serine protease stimulates melanization activation and expression of antimicrobial peptides in the Chinese oak silkworm, Antheraea pernyi

Lei Wang,Liu Yang,Xiao-San Zhou,Tao-Hong Li,Chao-Liang Liu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

Clip domain serine proteases (Clips) mediate various biological processes, especially in insect immune responses such as prophenoloxidase (proPO) activation and Toll pathway initiation. In the present study, we cloned and studied the functions of a clip domain serine protease from Antheraea pernyi (ApSnake). ApSnake contains a typical clip domain and a trypsin-like serine protease domain. It shares high similarity with serine protease snake in Bombyx mori, and groups into the CLIPC family. ApSnake mRNA expressions were detected in all tested tissues, particularly high in the hemocytes. Challenged with four different microorganisms (Escherichia coli, Beauveria bassiana, Micrococcus luteus or Nuclear polyhedrosis virus), the expressions of ApSnake mRNA were induced significantly compared with control, particularly challenged by B. bassiana and M. luteus. Recombinant ApSnake protein stimulated melanization reaction and prophenoloxidase activation in A. pernyi hemolymph, but had no effect on phenoloxidase activity. Injection of recombinant ApSnake into A. pernyi larvae increased the transcript levels of proPO and antimicrobial peptides (AMPs) in hemocytes significantly. Our results suggested that ApSnake might be a modulating protein that both stimulate the melanization activation process and AMPs synthesis, and plays an important role in the innate immunity of A. pernyi.

Five Flavors of Life

Liu Chun lei,Wang Lan Chuan 한국콘텐츠학회 2017 ICCC International Digital Design Invitation Exhib Vol.2017 No.8

Preliminary Studies on the Physiological and Biochemical Characteristics of Onion Growth

Liu Lei,Liu Shiqi,Xu Li,Qi Liandong 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Reduced graphene oxide/polypyrrole composite: an advanced electrode for high-performance symmetric/asymmetric supercapacitor

Liu Guijing,Shi Yanying,Wang Lei,Song Yadong,Gao Shanmin,Liu Dong,Fan Leqing 한국탄소학회 2020 Carbon Letters Vol.30 No.4

Polypyrrole (PPy) decorated on reduced graphene oxide (rGO) flms is successfully prepared with pyrrole (Py) monomers and rGO through one-step combining oxidation with polymerization reaction. Compared with the pure individual compo�nents, rGO/PPy compound turns out better electrochemical characteristics owing to the introduction of rGO sheets, which improves the specifc surface area and the conductivity of composite material. When the amount of rGO is 10% of the total, the rGO/PPy compound delivers the best capacitance of 389.3 F g−1 at 1.0 A g−1 in a three-electrode system and 266.8 F g−1 at 0.25 A g−1 in the symmetric supercapacitor system. In addition, asymmetric device (rGO/PPy//AC) has been success�fully fabricated using optimized rGO/PPy compound as positive electrode, activated carbon as negative electrode (AC) and 1 M Na2SO4 aqueous solution as electrolyte. The device obtains long cycle stability under the high-voltage region from 0 to 1.6 V, meanwhile displaying the satisfed energy density of 19.7 Wh kg−1 at 478.1 W kg−1. Besides, the rGO/PPy//AC device presents satisfactory rate capability and long life time.