http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
흰쥐에게 내독소의 비경구 투여가 혈청 및 간의 Alkaline Phosphatase 및 5'-Nucleotidase 활성도 변동에 미치는 영향
문교철,곽춘식,주일 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3
The activities of the cytosolic, microsomal and mitochondrial alkaline phosphatase(ALP) and 5'-nucleotidase(5'-NT) in the liver were measured in order to evaluate the cholestatic changes, one of the hepatic damages under the endotoxin administration. And the activities of these enzymes in the serum were also measured. For administration of endotoxin, a dose of 5mg of endotoxin(lipopolysaccharide E. coli 026: B6, from Sigma chemical company, USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3,8 and 24 hours of injection with endotoxin to measure the activities of the above enzymes in serum and their livers. Cyrtosolic and microsomal ALP and 5'-NT activities showed a significant increase between 3 and 8 hours after endotoxin administration. The activity of the mitochondrial ALP and 5'-NT in the liver showed no significant changes throughout the experiment. Serum ALP activity showed a increase without significace. But serum 5'-NT showed a significant increase at 8 hours after endotoxin administration. According to the results, it is suggested that cytosolic and microsomal ALP and 5'-NT activity is increased caused by increased biosynthesis and impairment of hepatic excretory function for these enzymes. And it is also suggested that increased activity of the serum ALP and 5'-NT is due to the reflux of these enzymes from the liver and due to leak into the blood through the damaged membrane of hepatocyte.
흰쥐 재생간의 Leucine Aminopeptidase의 활성치
문교철,곽춘식,김여희 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2
Changes in the activities of the followings have been studied over a period of 6 days after partial hepatectomy in rats: Plasma membrane, mitochondrial, nuclear and cytosolic leucine aminopeptidase and microsomal-particle bound aminopeptidase of the regenerating liver and serum leucine aminopeptidase. The activities of alkaliine phosphatase in the subcellular fractions were also measured. The activities of leucine aminopeptidase and alkaline phosphatase in serum markedly elevated from 12 hours to three days after partial hepatectomy in rats. The activity of plasma memberane-bound leucine aminopeptidase in the regenerating rat liver drastically increased during the first and the third days of the operation. And the activity of microsomal particle-bound aminopeptidase in the regenerating liver significantly increased between the second and the third days after operation. The activities of nuclear and cytosolic leucine aminopeptidase in the regenerating liver showed a substantial increase at the second day and from first to sixth day respectively after operation. However, no significant change in hepatic mitochondrial leucine aminopeptidase was noted throughout the experiments. The activities of plasma membrane, microsomal, mitochondrial, nuclear and cytosolic alkaline phosphatase in the regenerating liver markedly increased throughout the experiments.
곽춘식,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1
The possible mechanisms of increased aryl sulfotransferase (AST) isozymes activities in cholestatic rat liver were studied. Hepatic AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ activities were determined from the experimental rats with common bile duct ligation (CBDL). The Michaelis-Menten constants in these hepatic enzymes were also measured. The activities of mitochondrial AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ, and microsomal AST-Ⅲ, Ⅳ as well as their Vmax values were found to be increased significantly in CBDL plus taurocholic acid (TCA) injected group than in the control group, such as CBDL alone groups. However, their Km values in the experimental groups did not vary. The results suggest that TCA stimulates biosynthesis of the AST in the liver.
곽춘식,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2
Liver and serum rhodanese activities were determined in acute ethanol intoxicated rats with extrahepatic cholestasis induced by common bile duct ligation (CBD) to manifest the biochemical background of alcohol drinking hazard under the hepatobiliary disease. Liver cytosolic and microsomal rhodanese activities and these Vmax values in CBD ligated rats with acute ethanol intoxication were found to be decreased much more than that in CBD ligation alone. However, the difference of Km value on above hepatic enzyme was not found between the experimental groups. On the other hand, serum rhodanese activity in CBD ligated rats with acute ethanol intoxication was greater increased more than that in CBD ligation alone. These results indicate that the biosynthesis of the hepatic rhodanese decreases and the serum rhodanese activity increases in cholestasis combined with acute ethanol intoxication. reflecting damage of aggravated hapatocytic membrane. Accordingly, the resulting data supported the fact that alcoholic drinks were enzymologically harmful to the hepatobiliary disease.
흰쥐 재생간의 Malate Dehydrogenase의 활성치
김여희,곽춘식,문교철 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2
A study was made on the changes in the activities of the following during 6 post-perative days: Cytosolic and mitochondrial malate dehydrogenase of regenerating rat livers, and rat serum malate dehydrogenase after 70% (median and left lateral lobes) partial hepatectomy. The activities of lactate dehydrogenase and alanine aminotransferase in both serum and cytosol of regenerating rat liver were also measured. After parial hepatectomy in the rats, activity of serum malate dehydrogenase trmendously increased in in the span between the 12 hours and the second day. The activity of cytoslic malate dehydrogenase in the regenerating liver significantly increased from the first to the second day after partial hepatectomy. However, no significant changes in hepatic mitochondrial malate dehydrogenase was noted throughout the experiments. The activities of serum lactate dehydrogenase and alanine aminotransferase markedly elevated after partial hepatectomy. And activity of alanine aminotransferase significantly decreased in the hepatic cytosol after partial hepatectomy but hepatic cytosolic lactate dehydrogenase showed no change.
흰쥐 재생간의 Monoamine Oxidase 의 활성치
박은미,곽춘식,문교철,김여희 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2
This study was intended to investigate the changes of hepatic mitochondrial and microsomal monoamine oxidase(MAO) A and B activities after 70%(median and left lateral lobes) partial hepatectomy in rats. The activities of mitochondrial MAO A and B in the regenerating liver significantly increased between the second and the third days of the operation. However, the microsomal MAO A activity in regenerating liver showed a marked decrease from two days after operation.
Malondialdehyde Levels in Middle Ear Fluid from Patients of Otitis Media with Effusion
Mun, Kyo-Cheol,Kim, Deok-Jun Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.1
Otitis media with effusion (OME) is an inflammatory disease of the middle ear cleft. Oxygen free radicals have been implicated in a variety of inflammatory disorders. Oxygen free radicals may also be involved in the pathogenesis of OME. To evaluate the involvement of oxygen free radicals in the pathogenesis of OME, the level of malondialdehyde, which gives an index of lipid peroxidation by oxygen free radicals, was measured by the reaction with thiobarbituric acid. Malondialdehyde level in the middle ear fluid from the OME group was higher than that in the normal control group. Malondialdehyde level in the middle ear fluid from a mucoid subgroup was higher than that in the serous subgroup. Malondialdehyde levels in the middle ear fluid from the serous subgroup was significantly correlated with symptom duration. The Pearson correlation coefficient between malondialdehyde levels in the middle ear fluid from the serous subgroup and symptom duration was 0.842 (P<0.05). These results indicate that lipid peroxidation by oxygen free radicals may be involved in the pathogenesis of human OME.