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치아의 치수 줄기 세포와 치주 인대 줄기 세포의 석회화와 분화에 PRP가 미치는 영향
Ka-young Lee,Ji-young Park,Sang-won Moon,Jong-Tae Park,Dong-seol Lee,Heung-Joong Kim,JOO-CHEOL PARK 대한구강해부학회 2007 대한구강해부학회지 Vol.31 No.1
1 . INTRODUCTION Tissue engineering using stem cell has been studied extensively in attempt to regenerate the lost tooth ancVor damaged periodontal tissue. Although Platelet rich plasma(PRP) isolated from the blood has been applied to the implant surgery, the studies about the direct role of the PRP on the cell are not sufficient. To use PRP in clinic successfully, evaluation about effect of PRP on the cytological lev하 is req버red. n‘ PURPOSE The purpose of this study was to investigate the roles of PRP during the differentiation of dental pulp stem ceJls (DPSCs) and periodontal ligament stem cells (PDLSCs) at cytol맹ic and histologic level. Ill. MATERIALS AND METHOD 1. Cell isolation & primary c띠ture Dental p비,p cells and periodontal ligament cells were isolated from the exf,미 iated third molar of human who dose not have any systernic disease, respectively. The cells were divided into two groups ; PRP was added in one group experimental and was not added in the other group as a control. And the cells were cultured for 15 days. 2. PRP preparation. After taking blood from the 5 rats (male, 6 weeks old), PRP was isolated from the blood with thrombin by the centrifugation method. 3. Immunofluorescence Axioscope multi fluorescence rnicroscopic observation was performed using STRO-1 antibody which is mesenchymal stem ceJl marker to iden디 fy the stem cells in the culture 4. Alizarin-Red S staining At the time of 0, 5, 10 때d 15 days after the cell culture, the Alizarin Red-S stai미 ng was performed to evaluate the rruner때alization. 5. RT-PCR Analysis At the time of 0, 5, 10 없d 15 days after the cell c비ture, total RNA was isolated using Trizol reagent and the first strand cDNA was synthesized. And PCR was performed with the specific primers of DSPP, BSP, ALP, type 1 collagen, osteocalcin, osteonectin and GAPDH to evaluate the differentiation. IV. RESULT 1. Analysis of Immunofluorescence Sever외 stem cells specific to STRO-1 antibody were observed through the FITC in DPSC and PDLSC. 2. Analysis of Alizarin Red-S staining No rnineralization was obseπed before the cultur어 15 days in both control-DPSC group and PRP-DPSCs group. However, at the c띠tured 15 days, mineralization was obseπed in the PRP-PDLSCs group. 3. RT-PCR 1) In PRP-DPSCs group, expression of DSPP were increased compared to those of control group‘ 2) In PRP-PDLSCs group, expression of DSPP, ALP, osteocalcin were almost same cornpared to those of control group V. Conclusion The result may suggests the functional role of PRP in the differentiation 뻐d mineralization of DPSCs by the increase of DSPP.
Ka-young Lee,Hyun-Joo Kim,최용석,Dong-seol Lee,Heung-Joong Kim,Chang-Seop Lee,JOO-CHEOL PARK 대한구강해부학회 2005 대한구강해부학회지 Vol.29 No.2
The dental epithelium produces the first inductive sign외s for od이ltogenesis such as FGF8, BMP4 and other unidentified factors These signals induce the expression of homeobox genes such as Lhx6, Lhx7, Pax9 and Msx1 which trigger off a cascade of events that res비t in the formation of toα,h. The aim of this study was identified the basic possibility of biotooth formation through interaction of odontogenic epithelium and bone marrow stem cell instead of ectomesenchymal cell on the basis of these tissue interaction in 않rly tooth development Bone marrow stem cell extracted from ICR mouse 없ld odontogenic epithelium and skin epithelium isolated from homologous embryo 암e recombinated by tissue recombination technique. Induction of early development marker such as Lhx6, Lhx7, Msx1, Pax9 and Axin2 by recombinated population is ar때yzed by RT-PCR All the genes (Lhx6, Lhx7, Pax9, Msx1, and Axin2l used in our experiment were expressed by recombinated population of dental epithelium and bone marrow stem cell. In recombinated population of skin epithelium and bone marrow stem cell, the expression of genes except for Lhx6 was identified. In cultured bone marrow stem cell, Pax9 and Msx1 were expressed. These results suggest that bone marrow stem cell can be used to rutificial tooth formation. Also as for the role of epithelial factor in the tooth formation, understanding of precise interaction of epithelium and stem cell will be important in the success of biotooth formation
Kim, Ka Young,Lee, Hui Su,Seol, Geun Hee Pharmaceutical Society of Great Britain 2015 Journal of pharmacy and pharmacology Vol.67 No.8
<P>The acute lung injury (ALI) model is characterised by a severe acute inflammatory response in the lungs that represents the pathogenesis of acute respiratory distress syndrome (ARDS). In this study, we sought to elucidate the anti-inflammatory mechanism of eucalyptol in relation to tissue remodelling in acute lung inflammation.</P>
Chemosensitivity is controlled by p63 modification with ubiquitin-like protein ISG15.
Jeon, Young Joo,Jo, Mi Gyeong,Yoo, Hee Min,Hong, Se-Hoon,Park, Jung-Mi,Ka, Seung Hyeun,Oh, Kyu Hee,Seol, Jae Hong,Jung, Yong Keun,Chung, Chin Ha American Society for Clinical Investigation 2012 The Journal of clinical investigation Vol.122 No.7
<P>Identification of the cellular mechanisms that mediate cancer cell chemosensitivity is important for developing new cancer treatment strategies. Several chemotherapeutic drugs increase levels of the posttranslational modifier ISG15, which suggests that ISGylation could suppress oncogenesis. However, how ISGylation of specific target proteins controls tumorigenesis is unknown. Here, we identified proteins that are ISGylated in response to chemotherapy. Treatment of a human mammary epithelial cell line with doxorubicin resulted in ISGylation of the p53 family protein p63. An alternative splice variant of p63, δNp63α, suppressed the transactivity of other p53 family members, and its expression was abnormally elevated in various human epithelial tumors, suggestive of an oncogenic role for this variant. We showed that ISGylation played an essential role in the downregulation of δNp63α. Anticancer drugs, including doxorubicin, induced δNp63α ISGylation and caspase-2 activation, leading to cleavage of ISGylated δNp63α in the nucleus and subsequent release of its inhibitory domain to the cytoplasm. ISGylation ablated the ability of δNp63α to promote anchorage-independent cell growth and tumor formation in vivo as well to suppress the transactivities of proapoptotic p53 family members. These findings establish ISG15 as a tumor suppressor via its conjugation to δNp63α and provide a molecular rationale for therapeutic use of doxorubicin against δNp63α-mediated cancers.</P>
Hui Su Lee,Purum Kang,Ka Young Kim,Geun Hee Seol 대한생리학회-대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.2
<i>Foeniculum vulgare</i> Mill. (fennel) is used to flavor food, in cosmetics, as an antioxidant, and to treat microbial, diabetic and common inflammation. No study to date, however, has assessed the anti-inflam-matory effects of fennel in experimental models of inflammation. The aims of this study were to investigate the anti-inflammatory effects of fennel in model of lipopolysaccharide (LPS)-induced acute lung injury. Mice were randomly assigned to seven groups (n=7∼10). In five groups, the mice were intraperitoneally injected with 1% Tween 80-saline (vehicle), fennel (125, 250, 500<i>μ</i>l/kg), or dexame-thasone (1 mg/kg), followed 1 h later by intratracheal instillation of LPS (1.5 mg/kg). In two groups, the mice were intraperitoneally injected with vehicle or fennel (250<i>μ</i>l/kg), followed 1 h later by intra-tracheal instillation of sterile saline. Mice were sacrificed 4 h later, and bronchoalveolar lavage fluid (BALF) and lung tissues were obtained. Fennel significantly and dose-dependently reduced LDH activity and immune cell numbers in LPS treated mice. In addition fennel effectively suppressed the LPS-induced increases in the production of the inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha, with 500<i>μ</i>l/kg fennel showing maximal reduction. Fennel also significantly and dose-dependently reduced the activity of the proinflammatory mediator matrix metalloproteinase 9 and the immune modulator nitric oxide (NO). Assessments of the involvement of the MAPK signaling pathway showed that fennel significantly decreased the LPS-induced phosphorylation of ERK. Fennel effectively blocked the inflammatory processes induced by LPS, by regulating pro-inflammatory cytokine production, transcription factors, and NO.
Lee, Hui Su,Kang, Purum,Kim, Ka Young,Seol, Geun Hee The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.2
Foeniculum vulgare Mill. (fennel) is used to flavor food, in cosmetics, as an antioxidant, and to treat microbial, diabetic and common inflammation. No study to date, however, has assessed the anti-inflammatory effects of fennel in experimental models of inflammation. The aims of this study were to investigate the anti-inflammatory effects of fennel in model of lipopolysaccharide (LPS)-induced acute lung injury. Mice were randomly assigned to seven groups (n=7~10). In five groups, the mice were intraperitoneally injected with 1% Tween 80-saline (vehicle), fennel (125, 250, $500{\mu}l/kg$), or dexamethasone (1 mg/kg), followed 1 h later by intratracheal instillation of LPS (1.5 mg/kg). In two groups, the mice were intraperitoneally injected with vehicle or fennel ($250{\mu}l/kg$), followed 1 h later by intratracheal instillation of sterile saline. Mice were sacrificed 4 h later, and bronchoalveolar lavage fluid (BALF) and lung tissues were obtained. Fennel significantly and dose-dependently reduced LDH activity and immune cell numbers in LPS treated mice. In addition fennel effectively suppressed the LPS-induced increases in the production of the inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha, with $500{\mu}l/kg$ fennel showing maximal reduction. Fennel also significantly and dose-dependently reduced the activity of the proinflammatory mediator matrix metalloproteinase 9 and the immune modulator nitric oxide (NO). Assessments of the involvement of the MAPK signaling pathway showed that fennel significantly decreased the LPS-induced phosphorylation of ERK. Fennel effectively blocked the inflammatory processes induced by LPS, by regulating pro-inflammatory cytokine production, transcription factors, and NO.