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The normal electroretinogram in adult healthy Shih Tzu dogs using the HMsERG
June-sub Lee,Kyung-hee Kim,장하영,Bora Lee,Joon Young Kim,정순욱 대한수의학회 2009 Journal of Veterinary Science Vol.10 No.3
Electroretinography (ERG) is a reliable diagnostic tool for the diagnosis of retinal disease. It measures electric potentials occurring in the retina in response to light stimulation. In this study, we examined the normal electroretinogram using the Handheld Multispecies ERG (HMsERG) in Shih Tzu dogs. ERG recordings were performed in twelve eyes of six healthy Shih Tzu dogs. Dogs were anesthetized with a combination of medetomidine and ketamine. Proparacaine eye drops were also applied as a topical anesthetic. Tropicamide eye drops were applied for mydriasis. After 20 min of dark adaptation, we recorded the amplitudes and implicit times of the b-waves of the rod, standard rod and cone (Std R&C), highintensity rod and cone (Hi-int R&C), and cone systems, and responses of the cones and inner retina by flicker light stimulation (cone flicker). Results showed that mean the amplitudes of a-waves of Std R&C, Hi-int R&C, and the cone responses were 141.25 μV, 173.00 μV, and 12.92 μV, respectively. The b-waves of the rod responses ranged from 141.58 to 155.25 μV; the Std R&C was 314.75 μV, the Hi-int R&C was 329.42 μV, the cones were 37.75 μV, and the flicker responses were 64.08 μV. The b/a ratios for the Std R&C, Hi-int R&C, and the cone response were 2.29, 1.94, and 3.71, respectively. Mean implicit time of the a-wave of the Std R&C was 15.12 ms, of Hi-int R&C was 13.42 ms, and of the cone response was 7.22 ms. The b-wave of the rod responses ranged from 68.12 to 72.68 ms, of Std R&C were 37.28 ms, of Hi-int R&C were 41.90, of the cone responses were 38.12 ms, and of the cone flicker responses were 22.80 ms. We believe that these parameters can be used as reference “normal” ERGs ranges for Shih Tzu dogs using the HMsERG under medetomidine and ketamine anesthesia.
Effect of Arp2/3 Complex on Sperm Motility and Membrane Structure in Bovine
Lee, June-Sub,Park, Yoo-Jin,Kim, Jin,Rahman, Md. Saidur,Kwon, Woo-Sung,Yoon, Sung-Jae,You, Young-Ah,Pang, Myung-Geol The Korean Society of Animal Reproduction 2013 Reproductive & developmental biology Vol.37 No.4
Sperm capacitation refers to polymerization of filamentous (F)-actin from globular (G)-actin. While the role of actin-related protein 2/3 (Arp2/3) complex in actin polymerization is well appreciated, the underlying mechanism(s) and its relationship with capacitation are poorly understood. Therefore, to evaluate the potential role of Arp2/3 complex on capacitation, bovine spermatozoa were incubated with multiple doses (1, 10 and $100{\mu}M$) of CK-636, an inhibitor of Arp2/3 complex with heparin. The cellular localization of the Arp2/3 complex in spermatozoa was identified by immunohistochemistry, whereas western blot was also applied to detect the protein tyrosine phosphorylation of sperm proteins. Additionally, sperm motility and kinematic parameters were evaluated using a computer-assisted sperm analysis system. CK-636 resulted in significant changes in the ratio of Arp2/3 complex localization between acrosome and equatorial region of the spermatozoa. Short-term exposure of spermatozoa to $100{\mu}M$ of CK-636 significantly decreased sperm motility, however a non-detectable effect on protein tyrosine phosphorylation was observed during capacitation. On the basis of these results, we propose that Arp2/3 complex is associated with morphological changes during capacitation and compromised sperm motility.
자발적 균열치유작용을 위한 미생물군 분리 및 균열치유작용 검증
이윤 ( Lee Yun ),유연준 ( Yu Yeon-june ),이병재 ( Lee Byung-jae ),이효섭 ( Lee Hyo-sub ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.1
최근 자발적 균열 치유 콘크리트 구조물 개념이 선진국 중심으로 연구가 활발히 진행되고 있다. 이는 초기 건설비용은 높지만 생애주기비용(Life Cycle Cost, LCC) 관점에서 구조물의 초기성능 유지 및 보수 · 보강 비용 감소의 경제적 장점과 시공에 따른 공해 발생, 에너지 소비 및 이산화탄소 발생 등을 줄일 수 있는 친환경적인 효과의 부수적 장점이 있다. 본 연구는 자발적 균열 치유 콘크리트에 적용할 수 있는 미생물 자원 확보에 대한 기초 연구이며, 자연 시료에서 분리한 미생물의 시멘트 내부 생존력, 탄산칼슘 석출량 그리고 균열 치유 능력을 비교하여 자발적 균열 치유 콘크리트에 안정적으로 적용할 수 있는 미생물 자원 확보가 목표이다.
선택적 환원 촉매(SCR)에서 배출가스 특성에 따른 NO<SUB>x</SUB> 변환효율 및 환원제(NH₃) 분사량 최적화에 관한 실험적 연구
오준석(June-Seok Oh),윤여빈(Yu-Bin Yoon),송춘섭(Chun-Sub Song),박영준(Young-Joon Park),이성욱(Seang-Wock Lee),조용석(Yong-Seok Cho) 한국자동차공학회 2009 한국자동차공학회 부문종합 학술대회 Vol.2009 No.4
The selective catalytic reduction(SCR) system is potentially a highly-effective means of NOx reduction for diesel engine. Generally, SCR makes use of a reductant added to the exhaust gas that can be used for reacting NO and NO₂ to N₂. The reductant, ammonia(NH₃) generated from a liquid urea-water solution. De-NOx process in the SCR, the ideal ratio of NH₃ molecules to NOx molecules is 1:1 for high efficiency of NOx reduction. The SCR system has good NOx reduction performance in the temperature window of about 250 to 400℃, low space velocity also leads an increase of NOx reduction efficiency, due to long residence time in the catalyst. This paper reviews a laboratory study to evaluate the effects of NOx-NH₃ concentrations, gas temperature and space velocity on the NOx reduction efficiency of the SCR. The maximum reduction efficiency is indicated when the NH₃ to NOx ratio is 1.2 and the space velocity is 30,000 h?¹ in 300℃. However, under various exhaust conditions, NH₃ injection amount need to be controlled for a high NOx conversion efficiency because of reducing unnecessary NH₃ consumption. Based on results of the rig-test, this study suggests the control strategy of NH₃ injection for optimizing NOx reduction efficiency.
Choi, Yong-June,Kang, Kyung-Mun,Lee, Hong-Sub,Park, Hyung-Ho Elsevier 2015 THIN SOLID FILMS - Vol.583 No.-
<P><B>Abstract</B></P> <P>The structural, electrical, and optical properties of undoped ZnO, F-doped ZnO (ZnO:F), and Al-doped ZnO (ZnO:Al) thin films with two different thicknesses deposited by atomic layer deposition (ALD) were investigated to evaluate the electromagnetic interference shielding effectiveness (EMI-SE). A diluted fluoride hydroxide was used as a single reactant source for F doping in a ZnO matrix, and the F doping concentration was about 1at.% in the ZnO:F films. The fabrication of the ZnO:Al films was followed by the typical ALD method, and the Al doping concentration of about 2at.% was adjusted by the dopant deposition intervals of the ZnO:Al<SUB>2</SUB>O<SUB>3</SUB> precursor pulse cycle ratios, which were fixed at 19:1. The film thickness variations were controlled with 600 and 1600 total ALD cycles of approximately 100nm and 300nm, respectively. The carrier concentration of the films is monotonically increased in order of the undoped ZnO, ZnO:F, and ZnO:Al films. The EMI-SE values of the undoped ZnO, ZnO:F, and ZnO:Al films at 1GHz were 0.9dB, 2.6dB, and 6.0dB for ~100nm, and were 2.1dB, 9.7dB, and 13.1dB for ~300nm, respectively. In our work, the EMI-SE value was increased by the enhancement of both the carrier concentration and film thickness due to reflection via the free carrier scattering effect.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fluorine or aluminum doped ZnO thin films prepared by atomic layer deposition </LI> <LI> Electromagnetic interference shielding effectiveness (EMI-SE) of ZnO thin films </LI> <LI> Carrier concentration and film thickness enhanced the EMI-SE. </LI> <LI> The enhancement of EMI-SE was due to reflection via free carrier scattering effect. </LI> </UL> </P>
콘크리트 균열치유물질의 건조방식에 따른 균열치유성능 비교
유연준 ( Yu¸ Yeon-june ),이윤 ( Lee¸ Yun ),이병재 ( Lee¸ Byung-jae ),오일표 ( Oh¸ Il-pyo ),이효섭 ( Lee¸ Hyo-sub ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.2
균열을 저감 시키거나 스스로 균열을 치유할 수 있는 자발적 균열치유 콘크리트 구조물의 개념이 대두되면서 실제 건설 현장에서의 적용을 위한 연구가 미국, 유럽, 일본 등의 선진국을 중심으로 활발히 진행되고 있다. 자발적 균열 치유 콘크리트 기술은 미국, 유럽, 일본 등의 선진국을 중심으로 약 20여 년 전에 개념이 도입되면서 시작되었으며, 국내에는 2004년 이 후 그 개념이 소개되어 현재는 일부 기초연구가 진행되고 있으며, 현재까지 콘크리트 분야의 응용연구는 미약한 실정이다. 이 연구에서는 이러한 자발적 균열치유 미생물을 적용한 콘크리트 균열치유물질의 건조방식 차이에 의한 균열치유성능을 파악하여 실제 사용환경에서의 적용성을 검토하고자 하였다.
유연준 ( Yu¸ Yeon-june ),이윤 ( Lee¸ Yun ),이병재 ( Lee¸ Byung-jae ),오일표 ( Oh¸ Il-pyo ),이효섭 ( Lee¸ Hyo-sub ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.2
최근 들어 균열을 저감 시키거나 스스로 균열을 치유할 수 있는 자발적 균열치유 콘크리트 구조물의 개념이 대두되면서 실제 건설 현장에서의 적용을 위한 연구가 미국, 유럽, 일본 등의 선진국을 중심으로 활발히 진행되고 있다. 1969년 자발적 균열 치유 성능이 처음 소개되었으며, 1990년대에 자발적 균열 치유 콘크리트에 대한 연구를 시작으로 2000년대에 자기치유 재료 및 이를 이용한 콘크리트 분야에 상당히 많은 논문이 발표되고 있다. 자발적 균열 치유 콘크리트 기술은 미국, 유럽, 일본 등의 선진국을 중심으로 약 20여 년 전에 개념이 도입되면서 시작되었으며, 국내에는 2004년 이후 그 개념이 소개되어 현재는 일부 기초연구가 진행되고 있으며, 현재까지 콘크리트 분야의 응용연구는 미약한 실정이다. 이 연구에서는 이러한 자발적 균열치유 미생물의 온도의존성을 파악하여 실제 사용환경에서의 적용성을 검토하고자 하였다..