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Choi, Jene,Moon, Soo Young,Hong, Joon Pio,Song, Ji-young,Oh, Kyung Taek,Lee, Sang-wook Informa UK Ltd 2010 Cancer investigation Vol.28 No.5
<P><B>Epidermal growth factor (EGF) stimulates apoptosis in tumor cells depending on the expression levels of EGF receptor and Her2. We examined the protein levels in 22 different cancer cell lines and assessed the responses to EGF. EGF-induced cytotoxicity was not correlated with the levels of either EGFR or Her2. Fourteen cell lines exhibited decreased cell proliferation, whereas 293T cells did not display any noticeable changes and degraded transiently expressed EGFR following EGF treatment. EGF treatment resulted in significant tumor growth inhibition in some xenografts. Our data indicate that exogenous EGF treatment leads to growth inhibition rather than inducing tumor cell proliferation.</B></P>
Alteration of The Quaternary Structure of Human UDP-Glucose Dehydrogenase by a Double Mutation
Huh, Jae-Wan,Yang, Seung-Ju,Hwang, Eun-Young,Choi, Myung-Min,Lee, Hyun-Ju,Kim, Eun-A,Choi, Soo-Young,Choi, Jene,Hong, Hea-Nam,Cho, Sung-Woo Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
There are conflicting views for the polymerization process of human UDP-glucose dehydrogenase (UGDH) and no clear evidence has been reported yet. Based on crystal coordinates for Streptococcus pyogenes UGDH, we made double mutant A222Q/S233G. The double mutagenesis had no effects on expression, stability, and secondary structure. Interestingly, A222Q/S233G was a dimeric form and showed an UGDH activity, although it showed increased $K_m$ values for substrates. These results suggest that Ala222 and Ser233 play an important role in maintaining the hexameric structure and the reduced binding affinities for substrates are attributable to its altered subunit communication although quaternary structure may not be critical for catalysis.
Alteration of The Quaternary Structure of Human UDP-Glucose Dehydrogenase by a Double Mutation
( Jae Wan Huh ),( Seung Ju Yang ),( Eun Young Hwang ),( Myung Min Choi ),( Hyun Ju Lee ),( Eun A Kim ),( Soo Young Choi ),( Jene Choi ),( Hea Nam Hong ),( Sung Woo Cho ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5
Kim, Kyu-Rae,Choi, Jene,Hwang, Jeong-Eun,Baik, Young-Ae,Shim, Jeong Yeon,Kim, Yong Man,Robboy, Stanley J Blackwell Publishing Ltd 2010 Histopathology Vol.57 No.4
<P>Kim K-R, Choi J, Hwang J-E, Baik Y-A, Shim J Y, Kim Y M & Robboy S J(2010) <I>Histopathology</I><B>57</B>, 587–596<B>Endocervical-like (Müllerian) mucinous borderline tumours of the ovary are frequently associated with the <I>KRAS</I> mutation</B></P><P>Aims: </P><P>Clinicopathological aspects of the endocervical-like mucinous borderline tumour of the ovary (EMBT), including higher frequencies of bilaterality, endometriosis and hormone receptor reactivity, and often admixtures of various Müllerian-type epithelia, closely resembles endometrioid tumour more than mucinous borderline tumour of the intestinal type (IMBT). Thus, the aims of this study were to determine whether EMBT is really a subtype of mucinous borderline tumours, as shown in the current classification system, and to determine the best classification for EMBT.</P><P>Methods and results: </P><P>The clinicopathological and immunohistochemical features of 17 EMBTs were analysed, including oestrogen receptor (ER), progesterone receptor (PR), <I>PTEN</I>, cytokeratins (CK) 7 and 20, and <I>&bgr;-catenin</I>. Additionally, mutational analyses of the <I>KRAS</I> (exon 1) and <I>PTEN</I> genes (all nine exons) were performed in all cases, and the results were compared with literature findings for IMBT and endometrioid tumours. Twelve patients (71%) were confirmed histologically to have endometriosis in one or both ovaries. In seven cases, gradual transitions from endometriotic foci to the EMBT were identified. Immunohistochemically, all cases were reactive for ER and PR, with no nuclear expression of &bgr;-catenin. CK7 positivity was strong in all patients, whereas there was no reactivity for CK20. <I>PTEN</I> reactivity was diffuse in the nuclei of epithelial and underlying stromal cells. Sixty-nine per cent showed <I>KRAS</I> mutations in exon 1 and codon 12, but no <I>PTEN</I> mutation was identified in any of the nine exons.</P><P>Conclusion: </P><P>Our study suggests that EMBT has features of both mucinous and endometrioid tumours and is an additional tumour type arising in endometriosis. While clinicopathological features of EMBTs are closer to endometrioid tumours, they still have molecular characteristics closer to IMBTs.</P>
Lee, Hee Jin,Choi, Jene,Hwang, Tae Sook,Shong, Young Kee,Hong, Suck Joon,Gong, Gyungyub Williams Wilkins Co 2010 American journal of clinical pathology Vol.133 No.5
<P>With various methods, BRAF mutations have been detected in 73.4% to 87.1% of papillary thyroid carcinomas (PTCs) in Korea. We assessed the ability of allele-specific polymerase chain reaction using a dual priming oligonucleotide system, compared with direct sequencing and pyrosequencing, to detect BRAF mutations in fine-needle aspiration specimens from 85 patients undergoing thyroidectomy. Final pathologic diagnoses were 55 malignant lesions and 30 benign lesions. We detected BRAF mutations in 47 (90%) of 52 PTCs by at least 1 method. The sensitivity of the dual priming oligonucleotide system (88.5%) was slightly higher than that of direct sequencing (82.7%) and pyrosequencing (86.5%). The specificity and positive predictive value of all 3 methods were 100%. The dual priming oligonucleotide system is a simple, rapid, and reliable method for detecting BRAF mutations. This method may be a useful adjunct tool to improve the preoperative diagnostic accuracy of PTC in fine-needle aspiration biopsy specimens.</P>
Noh Jin Hee,Ahn Ji Yong,Choi Jene,Park Young Soo,Na Hee Kyong,Lee Jeong Hoon,Jung Kee Wook,Kim Do Hoon,Choi Kee Don,Song Ho June,Lee Gin Hyug,Jung Hwoon-Yong,Kim Jung Mogg 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.3
Background/Aims: Real-time polymerase chain reaction (RT-PCR) is a fast and simple method for the simultaneous detection of clarithromycin (CLR) resistance and Helicobacter pylori. We evaluated the effectiveness of RT-PCR compared to that of the rapid urease test (RUT) and assessed its value in verifying CLR resistance. Methods: A total of 70 specimens with confirmed H. pylori infection in culture were enrolled and analyzed in this prospective study. All specimens were subjected to RT-PCR assay using fluorescence melting peak signals to detect H. pylori infection and CLR resistances caused by either A2142G or A2143G mutations in the 23S ribosomal RNA gene (23S rRNA). The results were compared to those of RUT and antimicrobial susceptibility culturing tests to investigate the efficacy of RT-PCR. Results: Among the 70 specimens analyzed, the positivity rate was 97.1% (68/70) with RT-PCR and 82.9% (58/70) with RUT. CLR resistance (minimum inhibitory concentration >1.0 μg/mL) was confirmed in 18.6% (13/70), and fluorescence melting curve analysis showed that 84.6% (11/13) had point mutations in 23S rRNA. Ten specimens had only A2143G mutation, and one specimen contained both A2142G and A2143G mutations. Conclusions: RT-PCR assay was found to be more efficient than RUT in detecting H. pylori infection and could effectively verify CLR resistance compared to the antimicrobial susceptibility culturing test. Considering the high sensitivity and accessibility of RT-PCR method, it could be used to easily detect CLR-resistant H. pylori, thus helping clinicians select suitable treatment regimen and improve the eradication rate.